Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,276,940 G→T 8.8% F120L (TTC→TTA)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,276,9400GT 8.8% 1098.6 / 51.5 305F120L (TTC→TTA) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (123/155);  new base T (13/14);  total (136/169)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 309 total aligned reads displayed.

TTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCT  >  CP009273/4276793‑4277088
                                                                                                                                                   |                                                                                                                                                    
  ccGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAg                                                                                                                                                   <  2:872595/149‑1 (MQ=255)
   cGGCGAGAAGCCGCCGAAAATCACCGACTGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGc                                                                                                                                                  <  2:3329100/149‑1 (MQ=255)
    ggCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTCTTGGCGAAGCg                                                                                                                                                 >  1:797835/1‑149 (MQ=255)
       gagaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCa                                                                                                                                              <  2:4530296/149‑1 (MQ=255)
        agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGACGCGGCAg                                                                                                                                             >  1:1887775/1‑149 (MQ=255)
        agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAg                                                                                                                                             >  1:145573/1‑149 (MQ=255)
        agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAg                                                                                                                                             >  1:2194877/1‑149 (MQ=255)
        agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAg                                                                                                                                             <  2:3622631/149‑1 (MQ=255)
            gccgccGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGt                                                                                                                                         >  1:2827300/1‑149 (MQ=255)
            gccgccGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGt                                                                                                                                         <  2:4779937/149‑1 (MQ=255)
               gccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTcgc                                                                                                                                      <  1:3306644/149‑1 (MQ=255)
               gccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTcgc                                                                                                                                      >  2:792041/1‑149 (MQ=255)
               gccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTcgc                                                                                                                                      >  1:2013773/1‑149 (MQ=255)
                  gAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGt                                                                                                                                   >  2:892063/1‑149 (MQ=255)
                  gAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGt                                                                                                                                   >  2:3817636/1‑149 (MQ=255)
                    ataTCACCGAATGCACCGAGCCAATGCGGGCGCAGGCCAGCTTCTCAACCGCGGCTTCCGGCACCATCGGCATATAACTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGACGCGGCAGACGTCTCGGTGc                                                                                                                                 <  2:376526/147‑1 (MQ=255)
                    aaaTCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGc                                                                                                                                 <  1:2337592/149‑1 (MQ=255)
                        cACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGct                                                                                                                             >  1:4638171/1‑149 (MQ=255)
                        cACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGct                                                                                                                             <  1:1182211/149‑1 (MQ=255)
                           cGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCtt                                                                                                                          <  1:2934267/149‑1 (MQ=255)
                           cGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCtt                                                                                                                          <  2:3118504/149‑1 (MQ=255)
                            gAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCttt                                                                                                                         <  2:2154782/149‑1 (MQ=255)
                                   ccGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCCGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTCTAGCTg                                                                                                                  >  2:1259716/1‑149 (MQ=255)
                                   ccGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTg                                                                                                                  >  2:3171622/1‑149 (MQ=255)
                                      cgcCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGata                                                                                                               <  1:4284375/149‑1 (MQ=255)
                                       gcCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGatat                                                                                                              <  2:4585270/149‑1 (MQ=255)
                                        ccAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATg                                                                                                             >  1:1498463/1‑149 (MQ=255)
                                          aaTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGtt                                                                                                           <  1:47919/149‑1 (MQ=255)
                                          aaTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGtt                                                                                                           <  1:312235/149‑1 (MQ=255)
                                          aaTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGtt                                                                                                           <  1:1767481/149‑1 (MQ=255)
                                           aTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGttt                                                                                                          <  1:1367784/149‑1 (MQ=255)
                                           aTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGttt                                                                                                          >  2:4127761/1‑149 (MQ=255)
                                           aTGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGttt                                                                                                          >  2:3628149/1‑149 (MQ=255)
                                            tGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTg                                                                                                         <  2:740366/149‑1 (MQ=255)
                                            tGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTGAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTg                                                                                                         <  2:3217685/149‑1 (MQ=255)
                                                   gcAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctggc                                                                                                  >  1:2681290/1‑149 (MQ=255)
                                                      ggCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctggctgg                                                                                               <  2:2377145/149‑1 (MQ=255)
                                                        ccAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                             >  2:2534535/1‑149 (MQ=255)
                                                             aTCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCgtcgtc                                                                                        >  1:3969221/1‑149 (MQ=255)
                                                              tCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGtcgtcg                                                                                       >  1:2975097/1‑149 (MQ=255)
                                                                 cAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCt                                                                                    <  2:2102372/149‑1 (MQ=255)
                                                                   aCCGAGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTAGTCGCCTTc                                                                                  >  2:2803100/1‑149 (MQ=255)
                                                                     cgcgGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTccc                                                                                <  1:129080/149‑1 (MQ=255)
                                                                      gcgGCTTCCGGCACCATCGGCATATAAATCGCCACCACCTCACCTTGTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCa                                                                               <  2:1346661/149‑1 (MQ=255)
                                                                        ggCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAga                                                                             >  2:191283/1‑149 (MQ=255)
                                                                        ggCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAga                                                                             <  1:337858/149‑1 (MQ=255)
                                                                        ggCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAga                                                                             <  1:2284940/149‑1 (MQ=255)
                                                                        ggCTTCAGTCACCATCGGCATATACATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAga                                                                             <  1:2576269/149‑1 (MQ=255)
                                                                              cGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATgg                                                                       <  1:2929639/149‑1 (MQ=255)
                                                                                gCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCg                                                                     <  2:108526/149‑1 (MQ=255)
                                                                                  aCCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGt                                                                   >  1:1212242/1‑149 (MQ=255)
                                                                                  aCCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGt                                                                   <  2:4016220/149‑1 (MQ=255)
                                                                                  aCCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCAAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGt                                                                   >  1:2094415/1‑149 (MQ=255)
                                                                                   ccATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTa                                                                  >  1:4136809/1‑149 (MQ=255)
                                                                                    cATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTAc                                                                 <  2:1838002/149‑1 (MQ=255)
                                                                                       cGGCATATAAATCGCCACCACATCACCTTTTTTACTGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCTGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGAt                                                              <  1:3135298/149‑1 (MQ=255)
                                                                                       cGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGAt                                                              <  1:2049581/149‑1 (MQ=255)
                                                                                       cGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGAt                                                              <  1:919131/149‑1 (MQ=255)
                                                                                         gCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCg                                                            >  2:2209552/1‑149 (MQ=255)
                                                                                          cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGc                                                           <  2:1870863/149‑1 (MQ=255)
                                                                                          cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGc                                                           <  1:1461295/149‑1 (MQ=255)
                                                                                          cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGc                                                           <  1:3173051/149‑1 (MQ=255)
                                                                                          cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGc                                                           >  1:4713801/1‑149 (MQ=255)
                                                                                           atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                          >  1:4063954/1‑149 (MQ=255)
                                                                                           atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                          >  2:4053554/1‑149 (MQ=255)
                                                                                           atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                          >  2:1543879/1‑149 (MQ=255)
                                                                                           atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGAGATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                          >  1:3248623/1‑149 (MQ=255)
                                                                                              taAATCGCCACCGCATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtt                                                       >  1:4374360/1‑149 (MQ=255)
                                                                                              taAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtt                                                       >  2:4303464/1‑149 (MQ=255)
                                                                                               aaaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                      >  2:1744301/1‑149 (MQ=255)
                                                                                               aaaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                      <  1:102435/149‑1 (MQ=255)
                                                                                               aaaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                      <  2:59791/149‑1 (MQ=255)
                                                                                               aaaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                      <  2:3288960/149‑1 (MQ=255)
                                                                                                aaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtttt                                                     <  2:1153157/149‑1 (MQ=255)
                                                                                                aaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtttt                                                     >  1:3086857/1‑149 (MQ=255)
                                                                                                aaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtttt                                                     >  1:1558982/1‑149 (MQ=255)
                                                                                                  tCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCt                                                   <  2:1671473/149‑1 (MQ=255)
                                                                                                     ccacaaCATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGc                                                >  2:4062000/1‑149 (MQ=255)
                                                                                                       accacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAg                                              >  2:1165970/1‑149 (MQ=255)
                                                                                                       accacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAg                                              >  2:1408682/1‑149 (MQ=255)
                                                                                                             tCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                        >  1:3743010/1‑149 (MQ=255)
                                                                                                             tCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                        >  1:2121462/1‑149 (MQ=255)
                                                                                                                  tttgttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCaa                                   <  2:64027/149‑1 (MQ=255)
                                                                                                                    ttttAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAgg                                 >  1:647451/1‑149 (MQ=255)
                                                                                                                      ttAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCTGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCa                               <  2:2985683/149‑1 (MQ=255)
                                                                                                                        aaTGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGTGGTCAAGGCAGt                             <  1:780195/149‑1 (MQ=255)
                                                                                                                          tGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGttt                           <  1:3935789/149‑1 (MQ=255)
                                                                                                                           gCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTg                          >  1:2869166/1‑149 (MQ=255)
                                                                                                                              cAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgccg                       >  1:1803247/1‑149 (MQ=255)
                                                                                                                              cAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgccg                       >  2:2876598/1‑149 (MQ=255)
                                                                                                                                 cTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCa                    >  2:2671868/1‑149 (MQ=255)
                                                                                                                                   cGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGa                  <  2:3503591/149‑1 (MQ=255)
                                                                                                                                   cGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTGAGAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGa                  <  2:2478648/149‑1 (MQ=255)
                                                                                                                                       cAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCa              <  2:4164830/149‑1 (MQ=255)
                                                                                                                                       cAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCa              <  2:3903170/149‑1 (MQ=255)
                                                                                                                                        tgggTATTGGCGAAGCGGCAGTCGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAg             <  1:3535841/148‑1 (MQ=255)
                                                                                                                                         gggTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGc            >  2:4553825/1‑149 (MQ=255)
                                                                                                                                         gggTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGc            >  2:3973828/1‑149 (MQ=255)
                                                                                                                                            tATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTg         <  2:2681293/149‑1 (MQ=255)
                                                                                                                                              ttGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGcc       >  2:3607479/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCT  >  CP009273/4276793‑4277088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑