Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,290,080 G→T 100% I256I (ATC→ATA)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,290,0800GT99.3% 1072.0 / ‑2.3 315I256I (ATC→ATA) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (132/179);  total (134/181)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 315 total aligned reads displayed.

TGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTT  >  CP009273/4289934‑4290225
                                                                                                                                                  |                                                                                                                                                 
 gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCTTTTCGGTTAtt                                                                                                                                                <  1:25841/149‑1 (MQ=255)
  cAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAttt                                                                                                                                               <  1:1508488/149‑1 (MQ=255)
      tCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGccc                                                                                                                                           <  2:1106062/149‑1 (MQ=255)
       ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCt                                                                                                                                          <  1:1386119/149‑1 (MQ=255)
         gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                        <  2:4154040/149‑1 (MQ=255)
             gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACg                                                                                                                                    <  2:1030125/149‑1 (MQ=255)
               cTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGc                                                                                                                                  <  1:896158/149‑1 (MQ=255)
                tGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGca                                                                                                                                 >  2:4421860/1‑149 (MQ=255)
                 ggCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                >  2:4400625/1‑149 (MQ=255)
                 ggCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                <  1:2307496/149‑1 (MQ=255)
                 ggCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                >  1:3333461/1‑149 (MQ=255)
                 ggCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                >  2:3605397/1‑149 (MQ=255)
                 ggCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaa                                                                                                                                <  1:4371017/149‑1 (MQ=255)
                    gAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaacaa                                                                                                                             >  1:442211/1‑149 (MQ=255)
                    gAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaacaa                                                                                                                             >  1:441867/1‑149 (MQ=255)
                     aGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAt                                                                                                                            <  1:1678724/149‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAttt                                                                                                                          <  1:4752210/149‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAttt                                                                                                                          <  1:3506001/149‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAttt                                                                                                                          <  2:3123875/149‑1 (MQ=255)
                        aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                         >  1:4269196/1‑149 (MQ=255)
                        aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                         <  2:1840352/149‑1 (MQ=255)
                        aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                         <  1:1278968/149‑1 (MQ=255)
                        aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                         >  1:1568922/1‑149 (MQ=255)
                         aCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGt                                                                                                                        >  1:75282/1‑149 (MQ=255)
                           tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                      <  1:2055977/149‑1 (MQ=255)
                           tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                      >  1:1763303/1‑149 (MQ=255)
                            cATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                                                     <  1:1940487/149‑1 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                  >  1:3196914/1‑149 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                  >  1:411837/1‑149 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                  <  1:666563/149‑1 (MQ=255)
                                 gagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                >  2:1081758/1‑149 (MQ=255)
                                 gagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                >  1:1253412/1‑149 (MQ=255)
                                     gtgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGcc                                                                                                            >  2:377941/1‑149 (MQ=255)
                                     gtgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGcc                                                                                                            >  1:2739751/1‑149 (MQ=255)
                                       gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAg                                                                                                          >  1:3685547/1‑149 (MQ=255)
                                         cGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGaa                                                                                                        <  1:222284/149‑1 (MQ=255)
                                          gCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAAt                                                                                                       <  2:3718507/149‑1 (MQ=255)
                                            cGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCt                                                                                                     <  1:1695974/149‑1 (MQ=255)
                                             ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                                                    >  1:325492/1‑149 (MQ=255)
                                                 tGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                                >  1:2511991/1‑149 (MQ=255)
                                                         tGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGcc                                                                                        <  1:2105815/149‑1 (MQ=255)
                                                          ggACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCg                                                                                       >  1:1336185/1‑149 (MQ=255)
                                                           gACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGt                                                                                      <  2:1721080/149‑1 (MQ=255)
                                                              ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGa                                                                                   >  2:2443278/1‑149 (MQ=255)
                                                                gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaaa                                                                                 <  2:3364105/149‑1 (MQ=255)
                                                                  ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAgg                                                                               >  1:4572648/1‑149 (MQ=255)
                                                                     aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCAc                                                                            <  1:2803147/149‑1 (MQ=255)
                                                                           aCCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcgc                                                                      <  2:2234430/149‑1 (MQ=255)
                                                                           aCCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcgc                                                                      >  2:2954242/1‑149 (MQ=255)
                                                                            ccGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCt                                                                     >  1:3704314/1‑149 (MQ=255)
                                                                            ccGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCt                                                                     <  1:1971461/149‑1 (MQ=255)
                                                                             cGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCtt                                                                    >  1:4449414/1‑149 (MQ=255)
                                                                              ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTc                                                                   <  1:3557920/149‑1 (MQ=255)
                                                                               gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTcc                                                                  >  1:4524029/1‑149 (MQ=255)
                                                                               gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTcc                                                                  >  2:62077/1‑149 (MQ=255)
                                                                               gTCTGATCTCCGGCAGATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTcc                                                                  <  2:2806480/149‑1 (MQ=255)
                                                                                tCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                 <  1:2727151/149‑1 (MQ=255)
                                                                                  tGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                                                               <  1:3155930/149‑1 (MQ=255)
                                                                                  tGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                                                               >  1:241762/1‑149 (MQ=255)
                                                                                   gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAc                                                              >  1:4699694/1‑149 (MQ=255)
                                                                                   gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAc                                                              >  1:3626271/1‑149 (MQ=255)
                                                                                      ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGt                                                           <  1:611500/149‑1 (MQ=255)
                                                                                           gCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                      >  2:668932/1‑149 (MQ=255)
                                                                                           gCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                      >  2:775104/1‑149 (MQ=255)
                                                                                            cATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCa                                                     <  2:3933760/149‑1 (MQ=255)
                                                                                               atCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACtt                                                  <  1:3863299/149‑1 (MQ=255)
                                                                                                 cGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTa                                                <  2:3051564/149‑1 (MQ=255)
                                                                                                     ggCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                            <  2:237053/149‑1 (MQ=255)
                                                                                                      gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGTCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTg                                           >  1:1120516/1‑149 (MQ=255)
                                                                                                      gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTg                                           <  1:2915098/149‑1 (MQ=255)
                                                                                                       cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg                                          >  1:1245091/1‑149 (MQ=255)
                                                                                                        tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                         <  2:2052750/149‑1 (MQ=255)
                                                                                                        tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                         <  2:3136120/149‑1 (MQ=255)
                                                                                                         cccGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTg                                        <  2:12682/149‑1 (MQ=255)
                                                                                                            gcgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgcgc                                     <  2:3634129/149‑1 (MQ=255)
                                                                                                              gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCtt                                   >  2:3283891/1‑149 (MQ=255)
                                                                                                                caatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTc                                 >  1:1595245/1‑149 (MQ=255)
                                                                                                                     aatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgc                            >  2:4778013/1‑149 (MQ=255)
                                                                                                                     aatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgc                            >  1:4047351/1‑149 (MQ=255)
                                                                                                                     aatGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgc                            <  2:1587637/149‑1 (MQ=255)
                                                                                                                         cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaa                        <  2:1954071/149‑1 (MQ=255)
                                                                                                                         cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaa                        <  2:3977977/149‑1 (MQ=255)
                                                                                                                             cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaa                    <  2:289448/149‑1 (MQ=255)
                                                                                                                               gagaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaatc                  >  1:3000305/1‑149 (MQ=255)
                                                                                                                                agaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                 <  1:130172/149‑1 (MQ=255)
                                                                                                                                agaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                 <  1:537081/149‑1 (MQ=255)
                                                                                                                                   ttCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt              <  2:33775/149‑1 (MQ=255)
                                                                                                                                    tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATc             <  2:1177940/149‑1 (MQ=255)
                                                                                                                                     ctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg            <  2:2132802/149‑1 (MQ=255)
                                                                                                                                     ctcGCGTTGCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg            >  1:1085469/1‑149 (MQ=255)
                                                                                                                                      tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCATGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGt           <  2:232368/149‑1 (MQ=255)
                                                                                                                                      tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATAGACCGCCAGAATCTTTGACCGCGCGCCGTTGAAATGCAGTTGCGCTTCCAGACCGTTCGCAACTTTACTCTGTTGCGATTTCACCTCGTAATAAATCACATCGt           <  2:3266151/149‑1 (MQ=255)
                                                                                                                                       cgcgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTg          <  2:3169957/149‑1 (MQ=255)
                                                                                                                                        gcgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGa         <  2:2584480/149‑1 (MQ=255)
                                                                                                                                         cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAg        <  2:4291281/149‑1 (MQ=255)
                                                                                                                                         cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTGTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAg        <  2:1503844/149‑1 (MQ=255)
                                                                                                                                          gTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt       <  1:1801811/149‑1 (MQ=255)
                                                                                                                                            ttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGttt     <  1:3744033/149‑1 (MQ=255)
                                                                                                                                               ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGtt  >  2:4061625/1‑149 (MQ=255)
                                                                                                                                               ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGtt  <  2:1205787/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTT  >  CP009273/4289934‑4290225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑