Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 361,692 G→T 35.9% Q18K (CAG→AAG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273361,6920GT35.9% 8307.0 / inf 5443Q18K (CAG→AAG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1501/1981);  new base T (894/1064);  total (2398/3045)
Fisher's exact test for biased strand distribution p-value = 7.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5502 total aligned reads displayed.

GCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCT  >  CP009273/361544‑361840
                                                                                                                                                    |                                                                                                                                                    
  aaCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTAt                                                                                                                                                    >  1:2435090/1‑149 (MQ=255)
  aaCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGAt                                                                                                                                                    <  2:4314628/149‑1 (MQ=255)
  aaCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGGTGTGCCACGCGGTTGGGACTGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGACACGGTCTTAt                                                                                                                                                    >  2:1131276/1‑149 (MQ=255)
   aCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTtata                                                                                                                                                   >  2:4455888/1‑149 (MQ=255)
    cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATaa                                                                                                                                                  <  2:4547794/149‑1 (MQ=255)
     gCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAag                                                                                                                                                 <  1:978736/149‑1 (MQ=255)
     gCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAag                                                                                                                                                 >  2:3712944/1‑149 (MQ=255)
       cAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAagag                                                                                                                                               >  1:4419784/1‑149 (MQ=255)
       cAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAagag                                                                                                                                               >  1:2892011/1‑149 (MQ=255)
       cAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTACACTATTTCCCGCGTTGTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAACCGGTCTGATAagag                                                                                                                                               >  1:4349274/1‑149 (MQ=255)
        aaTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAgaga                                                                                                                                              <  2:1333721/149‑1 (MQ=255)
        aaTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAgaga                                                                                                                                              <  2:2821535/149‑1 (MQ=255)
         aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGac                                                                                                                                             <  2:2097989/149‑1 (MQ=255)
         aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGac                                                                                                                                             >  1:4176743/1‑149 (MQ=255)
           cAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGacac                                                                                                                                           <  1:3997616/149‑1 (MQ=255)
            aGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACAcc                                                                                                                                          >  2:481825/1‑149 (MQ=255)
            aGCAACGACTGTGTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACAcc                                                                                                                                          <  2:3232018/149‑1 (MQ=255)
                aCGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTACCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCAACACGCGGGAAACGGTCTGATAAGAGACACCGGCa                                                                                                                                      >  1:35774/1‑149 (MQ=255)
                  gACTTTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGTGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAATCGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATa                                                                                                                                    <  2:1512713/149‑1 (MQ=255)
                  gACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATa                                                                                                                                    <  2:3378463/149‑1 (MQ=255)
                   aCTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATAc                                                                                                                                   >  1:678138/1‑149 (MQ=255)
                   aCTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATAc                                                                                                                                   >  2:2955761/1‑149 (MQ=255)
                     tGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTTATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATActc                                                                                                                                 <  2:1050415/149‑1 (MQ=255)
                      gTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATActct                                                                                                                                >  1:3406321/1‑149 (MQ=255)
                      gTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATActct                                                                                                                                <  1:1838285/149‑1 (MQ=255)
                            ccGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACa                                                                                                                          <  2:2183573/149‑1 (MQ=255)
                             cGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACAt                                                                                                                         <  2:3288260/149‑1 (MQ=255)
                             cGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACAt                                                                                                                         <  1:4674775/149‑1 (MQ=255)
                              gCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATc                                                                                                                        >  1:3227300/1‑149 (MQ=255)
                              gCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATc                                                                                                                        <  1:448423/149‑1 (MQ=255)
                                cAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                                      >  2:3582624/1‑149 (MQ=255)
                                 aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGta                                                                                                                     <  1:3081992/149‑1 (MQ=255)
                                  gttgttGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGtat                                                                                                                    >  2:3471255/1‑149 (MQ=255)
                                      ttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTACACTTTTTACCGCGTTTTAGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAAGGTCTTATAAGAGACACAGGCATACACTGCGACATAGTATAACg                                                                                                                >  1:3726102/1‑149 (MQ=255)
                                       tgtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGt                                                                                                               <  1:1364009/149‑1 (MQ=255)
                                        gtgcgtcgcgGTTGGTTATGTAATTCAGCTCCGCCATCGCCGCTTCCAATTTTTCCCGCGTTTTCGCAGATACGTGGCTGGCCTGGTTCACCACGCGGGTAACGGTCTTATAAGAGACACCGGAATACTCTGCGACATCGTATAACGtt                                                                                                              <  1:2808434/143‑1 (MQ=255)
                                        gtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCTTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGtt                                                                                                              >  1:2622082/1‑149 (MQ=255)
                                        gtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGtt                                                                                                              <  1:272376/149‑1 (MQ=255)
                                             aCGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATTAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTgg                                                                                                         <  1:2005584/149‑1 (MQ=255)
                                               gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGtt                                                                                                       <  1:2178059/149‑1 (MQ=255)
                                                cgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCTTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGttt                                                                                                      <  1:2249735/149‑1 (MQ=255)
                                                  gTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTca                                                                                                    >  2:3051606/1‑149 (MQ=255)
                                                   ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcac                                                                                                   <  1:2971604/149‑1 (MQ=255)
                                                   ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcac                                                                                                   <  2:563496/149‑1 (MQ=255)
                                                      ggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAtt                                                                                                >  1:1000827/1‑149 (MQ=255)
                                                       gAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTc                                                                                               <  2:4617261/149‑1 (MQ=255)
                                                       gAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTc                                                                                               <  1:2324021/149‑1 (MQ=255)
                                                        aaTGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTca                                                                                              <  2:4391305/149‑1 (MQ=255)
                                                         aTGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCTTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcac                                                                                             >  1:3916509/1‑149 (MQ=255)
                                                          tGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacc                                                                                            >  2:2650587/1‑149 (MQ=255)
                                                           gTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacca                                                                                           >  1:3494193/1‑149 (MQ=255)
                                                           gTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacca                                                                                           <  1:3827121/149‑1 (MQ=255)
                                                            ttaTTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcaccac                                                                                          >  2:1773196/3‑149 (MQ=255)
                                                            tAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcaccac                                                                                          >  2:314041/1‑149 (MQ=255)
                                                                 cAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCtta                                                                                     <  1:864864/149‑3 (MQ=255)
                                                                        gCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGAct                                                                              <  2:1689587/149‑1 (MQ=255)
                                                                        gCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGAct                                                                              <  1:1612434/149‑1 (MQ=255)
                                                                         ccATCGCCGCTTCCACTTTTTCCCGCTTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActc                                                                             >  2:1594048/1‑149 (MQ=255)
                                                                          cATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActct                                                                            <  1:1679821/149‑1 (MQ=255)
                                                                           aTCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctc                                                                           <  2:3940660/149‑1 (MQ=255)
                                                                           aTCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGActctc                                                                           >  1:3149024/1‑149 (MQ=255)
                                                                              gccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCGTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACGGGTTTCACATTCACCACCCTGAATTGACTCTCTTc                                                                        <  2:2560697/149‑1 (MQ=255)
                                                                                cgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCg                                                                      >  1:1147676/1‑149 (MQ=255)
                                                                                   ttCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGgc                                                                   >  2:2364881/1‑149 (MQ=255)
                                                                                   ttCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgc                                                                   <  2:1415045/149‑1 (MQ=255)
                                                                                   ttCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgc                                                                   >  1:774386/1‑149 (MQ=255)
                                                                                      cACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCt                                                                <  2:2266024/149‑1 (MQ=255)
                                                                                        cTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAt                                                              <  1:1453254/149‑1 (MQ=255)
                                                                                         tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTCTAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCtctc                                                             >  1:3648474/1‑149 (MQ=255)
                                                                                         tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                                             >  2:2446050/1‑149 (MQ=255)
                                                                                          ttttCCCGCTTGTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCa                                                            <  2:1246193/149‑1 (MQ=255)
                                                                                              cccGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGcc                                                        <  2:272674/149‑1 (MQ=255)
                                                                                                cgcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCAt                                                      <  2:4027175/149‑1 (MQ=255)
                                                                                                   gTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATAcc                                                   >  1:1254723/1‑149 (MQ=255)
                                                                                                    ttttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcg                                                  >  1:60469/1‑149 (MQ=255)
                                                                                                         gCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGaaa                                             <  2:655187/149‑1 (MQ=255)
                                                                                                          cAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAg                                            <  2:802429/149‑1 (MQ=255)
                                                                                                           aGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAgg                                           <  1:520004/149‑1 (MQ=255)
                                                                                                            gAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                          >  2:4229199/1‑149 (MQ=255)
                                                                                                              aaCGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGttt                                        <  2:3648881/149‑1 (MQ=255)
                                                                                                              aaCGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGttt                                        <  1:2537876/149‑1 (MQ=255)
                                                                                                               aCGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGtttt                                       >  1:4102254/1‑149 (MQ=255)
                                                                                                                cGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTg                                      <  1:1534161/149‑1 (MQ=255)
                                                                                                                       ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt                               >  1:2102004/1‑149 (MQ=255)
                                                                                                                           tGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt                           >  1:3315968/1‑149 (MQ=255)
                                                                                                                           tGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt                           >  2:1989232/1‑149 (MQ=255)
                                                                                                                            ggTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATg                          <  2:3870716/149‑1 (MQ=255)
                                                                                                                                 accacGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc                     <  2:898342/149‑1 (MQ=255)
                                                                                                                                 accacGCGGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc                     <  2:412906/149‑1 (MQ=255)
                                                                                                                                    aCGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt                  >  1:1075481‑M2/1‑148 (MQ=255)
                                                                                                                                     cgcgGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACGGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtt                 >  2:468845‑M2/1‑147 (MQ=255)
                                                                                                                                       cgGGAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa               >  2:3796950‑M2/1‑145 (MQ=255)
                                                                                                                                       cgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa               >  1:475471‑M2/1‑145 (MQ=255)
                                                                                                                                        gggAAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat              >  1:4232974‑M2/1‑144 (MQ=255)
                                                                                                                                         ggAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatt             >  2:1893521‑M2/1‑143 (MQ=255)
                                                                                                                                          gAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta            >  1:1928820‑M2/1‑142 (MQ=255)
                                                                                                                                          gAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta            >  1:175762‑M2/1‑142 (MQ=255)
                                                                                                                                            aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttcattaaa          <  2:2887482‑M2/149‑10 (MQ=255)
                                                                                                                                             aCGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag         >  1:4564125‑M2/1‑139 (MQ=255)
                                                                                                                                              cGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaagg        >  2:727853‑M2/1‑138 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCT  >  CP009273/361544‑361840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑