Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,358,920 G→T 100% R147L (CGC→CTC)  fxsA → suppressor of F exclusion of phage T7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,358,9200GT97.6% 825.2 / 9.0 253R147L (CGC→CTC) fxsAsuppressor of F exclusion of phage T7
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (74/173);  total (77/176)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

CTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGC  >  CP009273/4358772‑4359064
                                                                                                                                                    |                                                                                                                                                
  aCTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGctcg                                                                                                                                                >  1:2971499/1‑149 (MQ=255)
   cTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGa                                                                                                                                               >  1:2454801/1‑149 (MQ=255)
    ttttATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGTTGCCGCATGTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGAc                                                                                                                                              <  2:3040047/149‑1 (MQ=255)
        aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGcc                                                                                                                                          <  1:4131080/149‑1 (MQ=255)
        aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGcc                                                                                                                                          >  1:2702248/1‑149 (MQ=255)
        aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGcc                                                                                                                                          >  1:336914/1‑149 (MQ=255)
        aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGcc                                                                                                                                          <  1:3752329/149‑1 (MQ=255)
          tGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCtt                                                                                                                                        <  2:1085861/149‑1 (MQ=255)
           gccgccGGTGCACAAGCATCTGACAGTGAAGTTGATGCCGCATGTGCGCTTGTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTg                                                                                                                                       <  2:3828729/149‑1 (MQ=255)
               ccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCCGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCa                                                                                                                                   <  1:1557112/149‑1 (MQ=255)
                cgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                  <  1:4511692/149‑1 (MQ=255)
                 ggTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATa                                                                                                                                 <  2:4742780/149‑1 (MQ=255)
                   tGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATaaa                                                                                                                               <  2:2711543/149‑1 (MQ=255)
                      aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            <  2:2147654/149‑1 (MQ=255)
                      aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            <  2:506121/149‑1 (MQ=255)
                      aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            >  1:2878500/1‑149 (MQ=255)
                      aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            <  1:2786041/149‑1 (MQ=255)
                      aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            <  2:2855097/149‑1 (MQ=255)
                       aaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACg                                                                                                                           <  1:3193134/149‑1 (MQ=255)
                        aaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGa                                                                                                                          <  1:4266992/149‑1 (MQ=255)
                        aaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGa                                                                                                                          <  2:265529/149‑1 (MQ=255)
                        aaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGa                                                                                                                          >  2:3298783/1‑149 (MQ=255)
                         aGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGAt                                                                                                                         <  1:2910523/149‑1 (MQ=255)
                          gCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATc                                                                                                                        <  1:1239583/149‑1 (MQ=255)
                           cATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCg                                                                                                                       <  2:4557765/149‑1 (MQ=255)
                             tCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGcc                                                                                                                     <  1:2860660/149‑1 (MQ=255)
                             tCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGcc                                                                                                                     <  2:4609833/149‑1 (MQ=255)
                             tCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGcc                                                                                                                     <  2:2842229/149‑1 (MQ=255)
                              cTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCa                                                                                                                    <  2:2310936/149‑1 (MQ=255)
                              cTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCa                                                                                                                    <  1:2825575/149‑1 (MQ=255)
                               tGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAg                                                                                                                   >  1:3484341/1‑149 (MQ=255)
                                gACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAgg                                                                                                                  <  1:996571/149‑1 (MQ=255)
                                 aCAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCTACCGCCTTGATCATAAAGACGATCGCCAGGa                                                                                                                 >  2:1968290/1‑149 (MQ=255)
                                  cAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGAt                                                                                                                <  2:3596779/149‑1 (MQ=255)
                                   aGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGAtt                                                                                                               <  2:486380/149‑1 (MQ=255)
                                        aGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGt                                                                                                          >  1:2899698/1‑149 (MQ=255)
                                        aGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGt                                                                                                          <  1:2297575/149‑1 (MQ=255)
                                             aTGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGaaa                                                                                                     <  2:3434128/149‑1 (MQ=255)
                                             aTGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGaaa                                                                                                     <  2:1382780/149‑1 (MQ=255)
                                             aTGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGATAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGaaa                                                                                                     <  2:3671613/149‑1 (MQ=255)
                                                ccGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGc                                                                                                  >  2:797839/1‑149 (MQ=255)
                                                ccGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGc                                                                                                  <  2:3123267/149‑1 (MQ=255)
                                                 cGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGcc                                                                                                 >  2:754748/1‑149 (MQ=255)
                                                    aTTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGa                                                                                              <  1:3064978/149‑1 (MQ=255)
                                                     tttGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGAt                                                                                             <  2:3400913/149‑1 (MQ=255)
                                                     tttGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGAACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGAt                                                                                             >  2:4704032/1‑149 (MQ=255)
                                                      ttGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGAtt                                                                                            <  1:784660/149‑1 (MQ=255)
                                                      tggcgcTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATAATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGAtt                                                                                            <  1:864473/147‑1 (MQ=255)
                                                       tGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTa                                                                                           <  2:1753873/149‑1 (MQ=255)
                                                           cTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTAtgtg                                                                                       >  1:4758455/1‑149 (MQ=255)
                                                             tttCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGCATTATGTGGt                                                                                     >  1:3562559/1‑149 (MQ=255)
                                                               tctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTa                                                                                   >  1:4746197/1‑149 (MQ=255)
                                                                 tcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATg                                                                                 >  2:4573201/1‑149 (MQ=255)
                                                                   gCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGcc                                                                               <  2:3942799/149‑1 (MQ=255)
                                                                   gCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGcc                                                                               >  1:2502795/1‑149 (MQ=255)
                                                                     aTGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACTATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAt                                                                             <  2:2003551/149‑1 (MQ=255)
                                                                     aTGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAt                                                                             >  2:1803210/1‑149 (MQ=255)
                                                                      tGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAtt                                                                            <  1:2470051/149‑1 (MQ=255)
                                                                      tGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAtt                                                                            >  1:2600323/1‑149 (MQ=255)
                                                                          tGGCGGCGGTTGTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGAGGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTcc                                                                        <  1:2092689/149‑1 (MQ=255)
                                                                              ggcggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTGGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCg                                                                    <  1:118882/149‑1 (MQ=255)
                                                                               gcggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGt                                                                   <  2:2971499/149‑1 (MQ=255)
                                                                                cggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCTACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtt                                                                  <  1:1968290/149‑1 (MQ=255)
                                                                                 ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttt                                                                 <  2:4050645/149‑1 (MQ=255)
                                                                                 ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttt                                                                 <  1:62966/149‑1 (MQ=255)
                                                                                 ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttt                                                                 <  1:4049410/149‑1 (MQ=255)
                                                                                  gTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                <  2:2648295/149‑1 (MQ=255)
                                                                                   ttttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGCCCGCCTTGATCATAACGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttttt                                                               <  1:1283046/149‑1 (MQ=255)
                                                                                       aGCGCCGGGACCGGTGGCGTTAATAATTTTGATGGTGAGTACCAGCGAAAGGATGATTAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGtt                                                           <  1:2395071/149‑1 (MQ=255)
                                                                                       aGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGtt                                                           <  1:2064434/149‑1 (MQ=255)
                                                                                        gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          >  2:596941/1‑149 (MQ=255)
                                                                                        gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          <  1:4253691/149‑1 (MQ=255)
                                                                                        gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          <  2:4348779/149‑1 (MQ=255)
                                                                                        gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          <  1:954919/149‑1 (MQ=255)
                                                                                          gcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTg                                                        <  1:2930128/149‑1 (MQ=255)
                                                                                          gcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTg                                                        <  1:1981485/149‑1 (MQ=255)
                                                                                            cGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgc                                                      >  2:2081779/1‑149 (MQ=255)
                                                                                             gggACCGTTGGCGGTAATACTTTTGATGGTGTGTACGAGCGAAAGGATGATGAGCTCGACCGCCTTTATCATAAAGACTATCGCCAGGATTAATGTCGAAACGCCGGATTATGTTGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgca                                                     <  1:39096/149‑1 (MQ=255)
                                                                                             gggACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACTATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgca                                                     >  1:3662078/1‑149 (MQ=255)
                                                                                             gggACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgca                                                     >  2:3158960/1‑149 (MQ=255)
                                                                                             gggACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgca                                                     >  2:1551072/1‑149 (MQ=255)
                                                                                               gACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgcagc                                                   >  2:1184844/1‑149 (MQ=255)
                                                                                                     tGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                             >  1:1727109/1‑149 (MQ=255)
                                                                                                     tGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                             <  2:3864613/149‑1 (MQ=255)
                                                                                                       gCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCa                                           <  1:3854032/149‑1 (MQ=255)
                                                                                                        cGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCAt                                          <  1:2760460/149‑1 (MQ=255)
                                                                                                              tACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAcccc                                    >  2:2425847/1‑149 (MQ=255)
                                                                                                                        ggTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGa                          >  2:372032/1‑149 (MQ=255)
                                                                                                                          tGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACTATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATc                        <  2:1480809/149‑1 (MQ=255)
                                                                                                                              tACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAgcg                    <  1:1475877/149‑1 (MQ=255)
                                                                                                                                ccAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCa                  >  1:3124877/1‑149 (MQ=255)
                                                                                                                                   gCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATag               >  1:1085108/1‑149 (MQ=255)
                                                                                                                                    cGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATaga              <  1:2630436/149‑1 (MQ=255)
                                                                                                                                    cGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATaga              <  1:2699956/149‑1 (MQ=255)
                                                                                                                                      aaaGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAgaga            >  1:4302705/1‑149 (MQ=255)
                                                                                                                                       aaGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGAc           <  1:4588173/149‑1 (MQ=255)
                                                                                                                                         ggATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACtt         <  2:296701/149‑1 (MQ=255)
                                                                                                                                          gatgatGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACttt        >  2:3213318/1‑149 (MQ=255)
                                                                                                                                             gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTcc     <  1:1309723/149‑1 (MQ=255)
                                                                                                                                                gaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGc  <  1:2345987/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGC  >  CP009273/4358772‑4359064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑