Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,376,178 C→A 100% E1004* (GAA→TAA)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,376,1780CA99.7% 1205.6 / ‑4.5 355E1004* (GAA→TAA) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (180/173);  total (180/175)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 363 total aligned reads displayed.

CGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCG  >  CP009273/4376033‑4376323
                                                                                                                                                 |                                                                                                                                                 
cGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCt                                                                                                                                                <  2:573393/149‑1 (MQ=255)
 ggTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa                                                                                                                                               <  2:4776876/149‑1 (MQ=255)
    gACCCATCTCAGCTGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTg                                                                                                                                            <  1:3251945/149‑1 (MQ=255)
    gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTg                                                                                                                                            >  1:137596/1‑149 (MQ=255)
    gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTg                                                                                                                                            >  1:4094914/1‑149 (MQ=255)
    gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTg                                                                                                                                            >  1:2529832/1‑149 (MQ=255)
        cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCAt                                                                                                                                        <  1:3551675/149‑1 (MQ=255)
        cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCAt                                                                                                                                        >  2:1064187/1‑149 (MQ=255)
          tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATGTCCGTCACTTCTTAGCTATTGGCATCg                                                                                                                                      <  2:1309811/149‑1 (MQ=255)
              aGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCAACAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAg                                                                                                                                  <  1:4103439/149‑1 (MQ=255)
                    gTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCg                                                                                                                            >  1:3783127/1‑149 (MQ=255)
                     tAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                           <  1:4611415/149‑1 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATc                                                                                                                       >  1:2876947/1‑149 (MQ=255)
                          aCGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACGTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCg                                                                                                                      >  2:100614/1‑149 (MQ=255)
                            gCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTc                                                                                                                    >  2:3365016/1‑149 (MQ=255)
                              aGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCaa                                                                                                                  >  1:279043/1‑149 (MQ=255)
                               gCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAac                                                                                                                 >  2:3738313/1‑149 (MQ=255)
                                 tCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac                                                                                                               <  1:3830171/149‑1 (MQ=255)
                                    aaaaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcaccac                                                                                                            <  1:1909068/149‑1 (MQ=255)
                                    aaaaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcaccac                                                                                                            >  1:4768509/1‑149 (MQ=255)
                                    aaaaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcaccac                                                                                                            >  1:4601726/1‑149 (MQ=255)
                                      aaTCTGAATCCCCTGTTGCAGATCCACCCGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc                                                                                                          >  2:1747774/1‑149 (MQ=255)
                                      aaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc                                                                                                          >  1:934267/1‑149 (MQ=255)
                                        tCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcgc                                                                                                        >  2:3546139/1‑149 (MQ=255)
                                        tCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcgc                                                                                                        <  2:506099/149‑1 (MQ=255)
                                        tCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcgc                                                                                                        >  2:2816041/1‑149 (MQ=255)
                                           gAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTg                                                                                                     >  2:86251/1‑149 (MQ=255)
                                             aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAc                                                                                                   >  2:1802124/1‑149 (MQ=255)
                                               ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                                 >  1:2373686/1‑149 (MQ=255)
                                               ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                                 >  1:437979/1‑149 (MQ=255)
                                                 ccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCATGACCGAg                                                                                               >  2:1991797/1‑149 (MQ=255)
                                                   tGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTc                                                                                             >  2:202958/1‑149 (MQ=255)
                                                    gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCa                                                                                            <  2:1497981/149‑1 (MQ=255)
                                                      tGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCaga                                                                                          <  1:4426403/149‑1 (MQ=255)
                                                        cAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagaga                                                                                        <  1:1222541/149‑1 (MQ=255)
                                                         aGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                       <  1:1338778/149‑1 (MQ=255)
                                                          gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGc                                                                                      >  1:1152429/1‑149 (MQ=255)
                                                            tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGa                                                                                    >  2:4252969/1‑149 (MQ=255)
                                                             ccacgaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc                                                                                   <  2:311227/149‑1 (MQ=255)
                                                             ccaccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc                                                                                   >  2:4353637/1‑149 (MQ=255)
                                                              caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAcc                                                                                  >  2:2667824/1‑149 (MQ=255)
                                                                 caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGt                                                                               <  2:3047157/149‑1 (MQ=255)
                                                                 caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGt                                                                               <  2:276975/149‑1 (MQ=255)
                                                                  aGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTCTCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                              >  1:4175321/1‑149 (MQ=255)
                                                                  aGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                              >  1:2030069/1‑149 (MQ=255)
                                                                   ggAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                             >  2:3304123/1‑149 (MQ=255)
                                                                    gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTCTTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                            >  2:3637351/1‑149 (MQ=255)
                                                                    gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                            <  2:2586472/149‑1 (MQ=255)
                                                                     aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGAt                                                                           <  1:2966520/149‑1 (MQ=255)
                                                                       gACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATaa                                                                         >  1:4246715/1‑149 (MQ=255)
                                                                       gACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATaa                                                                         >  1:1740434/1‑149 (MQ=255)
                                                                         cTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAAc                                                                       >  1:2091123/1‑149 (MQ=255)
                                                                          ttCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACt                                                                      <  1:4232966/149‑1 (MQ=255)
                                                                          ttCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATGTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACt                                                                      <  2:2275357/149‑1 (MQ=255)
                                                                             cGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCt                                                                   >  1:3876097/1‑149 (MQ=255)
                                                                                tGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCgg                                                                >  2:1359307/1‑149 (MQ=255)
                                                                                 gCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGt                                                               <  2:3475756/149‑1 (MQ=255)
                                                                                  ccGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGta                                                              <  1:2190211/149‑1 (MQ=255)
                                                                                   cGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaa                                                             <  2:3421147/149‑1 (MQ=255)
                                                                                      gTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaataa                                                          <  2:3960973/149‑1 (MQ=255)
                                                                                      gTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaataa                                                          <  2:1197089/149‑1 (MQ=255)
                                                                                       ttGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATaaa                                                         >  1:4671927/1‑149 (MQ=255)
                                                                                        tGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAAc                                                        <  2:4579105/149‑1 (MQ=255)
                                                                                            gATCACCAGCTAGCAGCGACGCGCTGCGTTGTGTAGTTTTTCCTTAACTTATTAGATATTGGCATCTGCTGGGGCCGGTATCGTCAACACCCGGCGAGTGACCGAGTATGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt                                                    <  1:74889/149‑1 (MQ=255)
                                                                                            gATCACCAGCGAGCTGCGTCGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt                                                    <  1:222831/149‑1 (MQ=255)
                                                                                            gATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt                                                    <  1:3416368/149‑1 (MQ=255)
                                                                                                aCCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGtt                                                <  2:3213955/149‑1 (MQ=255)
                                                                                                aCCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGtt                                                <  1:2448153/149‑1 (MQ=255)
                                                                                                aCCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGtt                                                >  2:745177/1‑149 (MQ=255)
                                                                                                   aGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCgg                                             >  1:4690/1‑149 (MQ=255)
                                                                                                    gCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGc                                            <  2:2523025/149‑1 (MQ=255)
                                                                                                     cGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCa                                           <  1:1818511/149‑1 (MQ=255)
                                                                                                        gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGa                                        <  1:3773032/149‑1 (MQ=255)
                                                                                                          agcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAtt                                      >  1:3634115/1‑149 (MQ=255)
                                                                                                           gcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                     <  1:1063299/149‑1 (MQ=255)
                                                                                                           gcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                     <  1:3324039/149‑1 (MQ=255)
                                                                                                           gcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                     <  2:3189138/149‑1 (MQ=255)
                                                                                                           gcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                     <  2:2876947/149‑1 (MQ=255)
                                                                                                           gcGACGCGCTGCGGTGAGCAGGATGTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                     <  1:67328/149‑1 (MQ=255)
                                                                                                             gACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc                                   >  1:544581/1‑149 (MQ=255)
                                                                                                               cgcgCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTAAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtctc                                 <  2:687533/149‑1 (MQ=255)
                                                                                                                gcgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGTTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                                <  1:1858906/149‑1 (MQ=255)
                                                                                                                     gCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACg                           >  2:1643115/1‑149 (MQ=255)
                                                                                                                      cGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACgg                          <  2:3863961/149‑1 (MQ=255)
                                                                                                                       ggTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                         <  1:1574703/149‑1 (MQ=255)
                                                                                                                        gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTc                        >  1:2661663/1‑149 (MQ=255)
                                                                                                                         tGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTcc                       <  1:4333927/149‑1 (MQ=255)
                                                                                                                          gAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTccc                      <  1:4043511/149‑1 (MQ=255)
                                                                                                                               ggATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCg                 >  2:2113518/1‑149 (MQ=255)
                                                                                                                                  tttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg              <  2:1418212/149‑1 (MQ=255)
                                                                                                                                   ttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGa             >  2:1864332/1‑149 (MQ=255)
                                                                                                                                    tCCGTCACTTCTGAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATACACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAt            <  2:2173181/149‑1 (MQ=255)
                                                                                                                                       gTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt         <  2:4094914/149‑1 (MQ=255)
                                                                                                                                          aCTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggt      >  1:1507592/1‑149 (MQ=255)
                                                                                                                                          aCTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggt      >  2:2362563/1‑149 (MQ=255)
                                                                                                                                          aCTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggt      >  2:2605255/1‑149 (MQ=255)
                                                                                                                                           cttcttAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggtg     >  1:2326205/1‑149 (MQ=255)
                                                                                                                                            ttcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggtgg    >  2:4312098/1‑149 (MQ=255)
                                                                                                                                             tcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgc   <  1:1459742/149‑1 (MQ=255)
                                                                                                                                              cttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGGTGATACACTGCTCGGTAATAAACGCCTTGTTCGGCACGAGTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgcg  >  2:2975819/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCG  >  CP009273/4376033‑4376323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑