Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,381,504 T→A 35.6% I28I (ATT→ATA)  orn → oligoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,381,5040TA35.6% 379.5 / inf 331I28I (ATT→ATA) ornoligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (142/70);  new base A (65/53);  total (207/124)
Fisher's exact test for biased strand distribution p-value = 4.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 337 total aligned reads displayed.

AAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGA  >  CP009273/4381358‑4381652
                                                                                                                                                  |                                                                                                                                                    
aaaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGc                                                                                                                                                    >  2:2038299/1‑149 (MQ=255)
 aaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGcc                                                                                                                                                   >  2:1613652/1‑149 (MQ=255)
 aaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGcc                                                                                                                                                   >  2:11027/1‑149 (MQ=255)
 aaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                                   >  1:3854217/1‑149 (MQ=255)
  aaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCa                                                                                                                                                  >  2:729661/1‑149 (MQ=255)
  aaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCa                                                                                                                                                  >  2:2409279/1‑149 (MQ=255)
  aaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCa                                                                                                                                                  >  2:4185538/1‑149 (MQ=255)
   aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCAc                                                                                                                                                 >  1:67595/1‑149 (MQ=255)
   aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCAc                                                                                                                                                 >  1:2733640/1‑149 (MQ=255)
     aTCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGc                                                                                                                                               >  1:2277681/1‑149 (MQ=255)
     aTCTATGAGACACGCAAGTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAAGAGCAGGAGTGCCAATGAAAACAACCTGATGTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGc                                                                                                                                               <  1:3003311/149‑1 (MQ=255)
      tCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCt                                                                                                                                              <  2:1205734/149‑1 (MQ=255)
        tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTgg                                                                                                                                            >  1:4525980/1‑149 (MQ=255)
        tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTgg                                                                                                                                            <  1:1791802/149‑1 (MQ=255)
         aTGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGt                                                                                                                                           >  2:4619034/1‑149 (MQ=255)
             tACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATTACCGGTCTGGATCCCGAGCGCGATCTCAATATTGAGATTGACACGCTGGTGAcc                                                                                                                                       >  1:4110868/1‑149 (MQ=255)
             tACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGAcc                                                                                                                                       >  2:2094144/1‑149 (MQ=255)
                acGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGAt                                                                                                                                    >  2:580458/1‑149 (MQ=255)
                      ttGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAAc                                                                                                                              >  1:237042/1‑149 (MQ=255)
                       tgtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAAcc                                                                                                                             <  1:223981/149‑1 (MQ=255)
                       tgtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAAcc                                                                                                                             >  2:3410211/1‑149 (MQ=255)
                         tgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTg                                                                                                                           >  2:432794/1‑149 (MQ=255)
                         tgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTg                                                                                                                           >  1:1709131/1‑149 (MQ=255)
                           gATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGaa                                                                                                                         <  1:1994066/149‑1 (MQ=255)
                           gATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCGGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGaa                                                                                                                         <  2:395020/149‑1 (MQ=255)
                               aaTATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                     >  1:4023336/1‑149 (MQ=255)
                                atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGACAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                                    <  2:753274/149‑1 (MQ=255)
                                atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                                    >  2:551019/1‑149 (MQ=255)
                                   taGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                                 >  1:4629320/1‑149 (MQ=255)
                                    aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGc                                                                                                                <  2:1889196/149‑1 (MQ=255)
                                     gTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCa                                                                                                               >  1:4558813/1‑149 (MQ=255)
                                      tCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAg                                                                                                              >  1:919914/1‑149 (MQ=255)
                                        aCTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCGGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGaa                                                                                                            <  2:1712862/149‑1 (MQ=255)
                                         cTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                           >  2:2649828/1‑149 (MQ=255)
                                         cTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                           >  1:4283513/1‑149 (MQ=255)
                                          tgtgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAgg                                                                                                          >  2:3783047/1‑149 (MQ=255)
                                           gtgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAggg                                                                                                         >  1:3069718/1‑149 (MQ=255)
                                            tgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                        <  1:3340027/149‑1 (MQ=255)
                                             gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGcc                                                                                                       <  2:866024/149‑1 (MQ=255)
                                              aaTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCg                                                                                                      <  1:572317/149‑1 (MQ=255)
                                              aaTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCg                                                                                                      <  1:2793185/149‑1 (MQ=255)
                                               aTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGa                                                                                                     <  1:4330920/149‑1 (MQ=255)
                                               aTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGa                                                                                                     >  1:1441636/1‑149 (MQ=255)
                                                   gTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                                 <  2:4376997/149‑1 (MQ=255)
                                                   gTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                                 <  1:4178659/149‑1 (MQ=255)
                                                       aaaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                             >  1:1172716/1‑149 (MQ=255)
                                                       aaaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                             >  1:3561638/1‑149 (MQ=255)
                                                        aaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAg                                                                                            >  1:1321151/1‑149 (MQ=255)
                                                         aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGt                                                                                           >  2:3602006/1‑149 (MQ=255)
                                                                 gAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGt                                                                                   >  2:4031854/1‑149 (MQ=255)
                                                                  aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTc                                                                                  <  2:4738303/149‑1 (MQ=255)
                                                                  aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATGGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTc                                                                                  <  2:4000696/149‑1 (MQ=255)
                                                                          tGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAAc                                                                          >  1:3311468/1‑149 (MQ=255)
                                                                           gAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACa                                                                         <  1:2445688/149‑1 (MQ=255)
                                                                              aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                      >  2:2952148/1‑149 (MQ=255)
                                                                              aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                      >  2:135643/1‑149 (MQ=255)
                                                                              aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                      >  2:2529035/1‑149 (MQ=255)
                                                                                    cTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCt                                                                <  2:3764568/149‑1 (MQ=255)
                                                                                     tGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTg                                                               >  2:3011932/1‑149 (MQ=255)
                                                                                      gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTga                                                              >  1:899275/1‑149 (MQ=255)
                                                                                      gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTga                                                              >  2:4559573/1‑149 (MQ=255)
                                                                                      gATTTGGATCGATCTTGAAATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTga                                                              <  2:2633435/149‑1 (MQ=255)
                                                                                       aTTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgat                                                             >  2:4223600/1‑149 (MQ=255)
                                                                                        tttGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatg                                                            >  1:2189031/1‑149 (MQ=255)
                                                                                          tGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgga                                                          <  2:2150537/149‑1 (MQ=255)
                                                                                          tGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGAGTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgga                                                          <  1:2598274/149‑1 (MQ=255)
                                                                                            gatcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggatg                                                        >  2:1013042/1‑149 (MQ=255)
                                                                                            gatcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggatg                                                        >  1:268226/1‑149 (MQ=255)
                                                                                             atcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGa                                                       >  1:3640433/1‑149 (MQ=255)
                                                                                               cgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACt                                                     <  2:3888901/149‑1 (MQ=255)
                                                                                                 atcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                   <  2:3626851/149‑1 (MQ=255)
                                                                                                 atcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                   >  1:80364/1‑149 (MQ=255)
                                                                                                 atcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                   >  1:2473642/1‑149 (MQ=255)
                                                                                                  tcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGTATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGa                                                  >  2:2140972/1‑149 (MQ=255)
                                                                                                   cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGaa                                                 >  2:3203903/1‑149 (MQ=255)
                                                                                                   cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGaa                                                 <  1:359648/149‑1 (MQ=255)
                                                                                                      gagaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGt                                              >  1:3208824/1‑149 (MQ=255)
                                                                                                       agaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                             >  1:4450630/1‑149 (MQ=255)
                                                                                                        gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgc                                            >  2:2769631/1‑149 (MQ=255)
                                                                                                        aaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgc                                            >  2:928716/2‑149 (MQ=255)
                                                                                                         aTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcg                                           <  1:1000875/149‑1 (MQ=255)
                                                                                                           gACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCa                                         >  2:4444411/1‑149 (MQ=255)
                                                                                                                 tCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATa                                   >  1:2235544/1‑149 (MQ=255)
                                                                                                                   tGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                                 >  2:458486/1‑149 (MQ=255)
                                                                                                                    ggATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCg                                >  2:65033/1‑149 (MQ=255)
                                                                                                                      aTCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGcc                              <  2:3270992/149‑1 (MQ=255)
                                                                                                                      aTCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGcc                              <  1:2150215/149‑1 (MQ=255)
                                                                                                                       tCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCa                             >  2:4635729/1‑149 (MQ=255)
                                                                                                                        cccGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAg                            <  2:1937536/149‑1 (MQ=255)
                                                                                                                          cGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCg                          <  1:3696583/149‑1 (MQ=255)
                                                                                                                          cGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCg                          <  1:746063/149‑1 (MQ=255)
                                                                                                                           gAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCgg                         >  2:2330057/1‑149 (MQ=255)
                                                                                                                             gcgcGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGACCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGc                       <  1:3933018/149‑1 (MQ=255)
                                                                                                                                 gATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGt                   <  1:1613652/149‑1 (MQ=255)
                                                                                                                                 gATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGt                   <  2:4525980/149‑1 (MQ=255)
                                                                                                                                  aTCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa                  >  1:651884/1‑149 (MQ=255)
                                                                                                                                       attattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgcg             >  1:3783050/1‑149 (MQ=255)
                                                                                                                                               gaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAg     <  1:1062783/149‑1 (MQ=255)
                                                                                                                                               gaTAGCCACGCTGGTGACCGATGCCAACCTGAATAGTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAg     <  1:3450818/149‑1 (MQ=255)
                                                                                                                                                 ttGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCg   <  1:3152087/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGA  >  CP009273/4381358‑4381652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑