Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,388,902 G→T 100% A558A (GCG→GCT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,388,9020GT100.0% 1116.4 / NA 325A558A (GCG→GCT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (168/157);  total (168/157)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 325 total aligned reads displayed.

TTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACA  >  CP009273/4388756‑4389045
                                                                                                                                                  |                                                                                                                                               
    gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTgg                                                                                                                                           >  1:2893121/1‑149 (MQ=255)
      aTTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAt                                                                                                                                         <  2:1826799/149‑1 (MQ=255)
       ttGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAtt                                                                                                                                        <  1:760508/149‑1 (MQ=255)
       ttGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAtt                                                                                                                                        <  1:3141293/149‑1 (MQ=255)
        tGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTg                                                                                                                                       >  2:21990/1‑149 (MQ=255)
         gATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGc                                                                                                                                      <  1:1459402/149‑1 (MQ=255)
           tttCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCAc                                                                                                                                    >  2:3498356/1‑149 (MQ=255)
              ccAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGaa                                                                                                                                 >  1:418285/1‑149 (MQ=255)
                 gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                              >  1:15206/1‑149 (MQ=255)
                 gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                              >  2:3287688/1‑149 (MQ=255)
                 gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                              <  2:512532/149‑1 (MQ=255)
                  tCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCt                                                                                                                             >  2:218591/1‑149 (MQ=255)
                      aTGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtgatg                                                                                                                         >  1:1599172/1‑149 (MQ=255)
                            aGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGaa                                                                                                                   <  1:3412706/149‑1 (MQ=255)
                             gCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAAc                                                                                                                  >  1:1701268/1‑149 (MQ=255)
                                  tgACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATGTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATgcg                                                                                                             <  2:1695003/149‑1 (MQ=255)
                                   gACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATgcgc                                                                                                            >  2:4535856/1‑149 (MQ=255)
                                         cAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGt                                                                                                      <  2:4772289/149‑1 (MQ=255)
                                          aGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTc                                                                                                     <  2:402089/149‑1 (MQ=255)
                                           gggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCa                                                                                                    >  2:2689176/1‑149 (MQ=255)
                                             gCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAAt                                                                                                  <  2:2056897/149‑1 (MQ=255)
                                              cAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATg                                                                                                 <  2:2513112/149‑1 (MQ=255)
                                                gTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                               >  1:615722/1‑149 (MQ=255)
                                                gTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                               >  1:1800445/1‑149 (MQ=255)
                                                   ccTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcaca                                                                                            >  1:443700/1‑149 (MQ=255)
                                                     tttACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAgg                                                                                          >  1:4303607/1‑149 (MQ=255)
                                                      ttACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGc                                                                                         <  1:2133983/149‑1 (MQ=255)
                                                          ccTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATa                                                                                     >  2:600610/1‑149 (MQ=255)
                                                          ccTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATa                                                                                     >  1:3605368/1‑149 (MQ=255)
                                                          ccTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATa                                                                                     >  2:600837/1‑149 (MQ=255)
                                                           cTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATaa                                                                                    >  1:504489/1‑149 (MQ=255)
                                                               cGCCAACAAAATTTACAAATATTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCct                                                                                <  2:1447891/149‑1 (MQ=255)
                                                                gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATGGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctg                                                                               >  1:1620314/1‑149 (MQ=255)
                                                                gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctg                                                                               >  1:383614/1‑149 (MQ=255)
                                                                gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctg                                                                               >  1:1013669/1‑149 (MQ=255)
                                                                 ccAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                              <  1:4063125/149‑1 (MQ=255)
                                                                 ccAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                              <  1:3546719/149‑1 (MQ=255)
                                                                 ccAACAAAATTTACAAATCTTGAGTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                              <  1:4615424/149‑1 (MQ=255)
                                                                  caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgct                                                                             <  2:3112134/149‑1 (MQ=255)
                                                                  caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgct                                                                             <  2:4636674/149‑1 (MQ=255)
                                                                    acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTgg                                                                           >  1:946354/1‑149 (MQ=255)
                                                                     caaAATTTACAACTCTTGATTCCTGAACTGATAGGCGACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGc                                                                          <  1:3906015/149‑1 (MQ=255)
                                                                     caaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGc                                                                          >  1:111455/1‑149 (MQ=255)
                                                                      aaaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCg                                                                         >  1:2056892/1‑149 (MQ=255)
                                                                       aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCgg                                                                        <  1:3766704/149‑1 (MQ=255)
                                                                          tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                     >  2:4213545/1‑149 (MQ=255)
                                                                          tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                     >  2:210352/1‑149 (MQ=255)
                                                                          tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                     <  2:1868698/149‑1 (MQ=255)
                                                                           ttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGt                                                                    <  1:486941/149‑1 (MQ=255)
                                                                             aCAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTgg                                                                  >  1:2930985/1‑149 (MQ=255)
                                                                              cAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGa                                                                 >  1:3689853/1‑149 (MQ=255)
                                                                                aaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGTAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAAc                                                               <  2:1981466/149‑1 (MQ=255)
                                                                                aaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAAc                                                               <  2:124787/149‑1 (MQ=255)
                                                                                aaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAAc                                                               >  2:2023126/1‑149 (MQ=255)
                                                                                    ttGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGtt                                                           >  2:3227530/1‑149 (MQ=255)
                                                                                     tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                          <  1:2607514/149‑1 (MQ=255)
                                                                                     tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                          <  1:2428150/149‑1 (MQ=255)
                                                                                     tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                          <  1:214812/149‑1 (MQ=255)
                                                                                     tGAGTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                          <  2:3439078/149‑1 (MQ=255)
                                                                                       aTTCCTGAACTGATAGGCTTCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATg                                                        <  1:3380921/149‑1 (MQ=255)
                                                                                         tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTc                                                      <  1:2197410/149‑1 (MQ=255)
                                                                                           cTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCg                                                    >  2:4686383/1‑149 (MQ=255)
                                                                                           cTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCg                                                    >  2:696531/1‑149 (MQ=255)
                                                                                             gAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCa                                                  <  1:4693587/149‑1 (MQ=255)
                                                                                              aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCaa                                                 >  1:1938353/1‑149 (MQ=255)
                                                                                                 tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                              >  2:3454234/1‑149 (MQ=255)
                                                                                                 tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                              >  2:298992/1‑149 (MQ=255)
                                                                                                 tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                              >  2:1065265/1‑149 (MQ=255)
                                                                                                  gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                             <  2:3914419/149‑1 (MQ=255)
                                                                                                  gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATAGTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                             <  2:3914374/149‑1 (MQ=255)
                                                                                                      ggCTACCTGGCGAAGCAGTCCGTATTCGAACCGGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaa                                         <  2:4349081/149‑1 (MQ=255)
                                                                                                         tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAc                                      <  2:2370718/149‑1 (MQ=255)
                                                                                                         tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAc                                      <  1:466925/149‑1 (MQ=255)
                                                                                                            cTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                   >  1:4526117/1‑149 (MQ=255)
                                                                                                             tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                  >  2:3004787/1‑149 (MQ=255)
                                                                                                             tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                  >  2:2465348/1‑149 (MQ=255)
                                                                                                             tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACACGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                  >  1:1374198/1‑149 (MQ=255)
                                                                                                              ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGCTCAATGGCACAGGCCATAACACTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccgc                                 >  1:4094274/1‑149 (MQ=255)
                                                                                                                  aaGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCggg                             >  2:3205501/1‑149 (MQ=255)
                                                                                                                   aGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                            >  2:3384403/1‑149 (MQ=255)
                                                                                                                    gCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtg                           <  1:190644/149‑1 (MQ=255)
                                                                                                                     cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtgg                          <  1:1978566/149‑1 (MQ=255)
                                                                                                                         ccGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTg                      <  1:4633606/149‑1 (MQ=255)
                                                                                                                          cGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                     <  2:1924420/149‑1 (MQ=255)
                                                                                                                          cGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                     <  2:3799892/149‑1 (MQ=255)
                                                                                                                                cGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAAt               <  2:15206/149‑1 (MQ=255)
                                                                                                                                 gAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATc              <  2:2634807/149‑1 (MQ=255)
                                                                                                                                  aaCCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt             <  2:686557/149‑1 (MQ=255)
                                                                                                                                  aaCCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt             >  1:2322302/1‑149 (MQ=255)
                                                                                                                                   aNCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTGGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg            >  2:4114683/1‑149 (MQ=255)
                                                                                                                                    ccTGGCAATATTGCTCAGTGGATTGCACGAAATCCGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGt           <  2:1599478/149‑1 (MQ=255)
                                                                                                                                      tGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTg         >  2:3078408/1‑149 (MQ=255)
                                                                                                                                       ggCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGa        >  1:2722578/1‑149 (MQ=255)
                                                                                                                                       ggCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGa        >  2:2813085/1‑149 (MQ=255)
                                                                                                                                        gCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAt       <  2:4744314/149‑1 (MQ=255)
                                                                                                                                          aaTATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACTGCCCATAACCCTGGTGGCGGACGTGGAACGGTTATGTCCGAAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt     <  1:4024847/149‑1 (MQ=255)
                                                                                                                                           atatTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCGATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa    >  1:3363216/1‑149 (MQ=255)
                                                                                                                                            tatTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTAc   >  1:997932/1‑149 (MQ=255)
                                                                                                                                             atTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa  <  2:1188034/149‑1 (MQ=255)
                                                                                                                                             atTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa  >  1:1290315/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACA  >  CP009273/4388756‑4389045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑