Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,406,553 C→A 35.4% R12L (CGG→CTG)  bsmA ← bioflm peroxide resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,406,5530CA35.4% 440.0 / 254.4 283R12L (CGG→CTG) bsmAbioflm peroxide resistance protein
Reads supporting (aligned to +/- strand):  ref base C (73/109);  new base A (42/59);  total (115/168)
Fisher's exact test for biased strand distribution p-value = 9.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

GTGCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAA  >  CP009273/4406406‑4406700
                                                                                                                                                   |                                                                                                                                                   
gTGCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCg                                                                                                                                                    >  2:2252507/1‑149 (MQ=255)
  gCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGt                                                                                                                                                  >  1:524891/1‑149 (MQ=255)
   cTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTa                                                                                                                                                 <  2:490836/149‑1 (MQ=255)
     tACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTaaa                                                                                                                                               >  1:2544612/1‑149 (MQ=255)
      aCGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAAt                                                                                                                                              >  2:4161582/1‑149 (MQ=255)
       cGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAGATa                                                                                                                                             >  2:511606/1‑149 (MQ=255)
        gCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATaa                                                                                                                                            <  1:4231371/149‑1 (MQ=255)
        gCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATaa                                                                                                                                            <  2:4707090/149‑1 (MQ=255)
         cTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAAc                                                                                                                                           <  1:3596999/149‑1 (MQ=255)
         cTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAAc                                                                                                                                           <  1:3925129/149‑1 (MQ=255)
             cTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTa                                                                                                                                       <  1:4008589/149‑1 (MQ=255)
             cTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTa                                                                                                                                       >  2:2418875/1‑149 (MQ=255)
              tATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTAt                                                                                                                                      <  1:1488976/149‑1 (MQ=255)
              tATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTAt                                                                                                                                      >  2:4663138/1‑149 (MQ=255)
               aTTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTAtt                                                                                                                                     <  2:104804/149‑1 (MQ=255)
               aTTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTAtt                                                                                                                                     >  2:2900559/1‑149 (MQ=255)
                ttCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCACACCGGTAAATAACGCTATTa                                                                                                                                    >  1:3467312/1‑149 (MQ=255)
                 tCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTAc                                                                                                                                   >  2:2315027/1‑149 (MQ=255)
                 tCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTAc                                                                                                                                   >  1:4669416/1‑149 (MQ=255)
                 tCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTAc                                                                                                                                   <  2:2673986/149‑1 (MQ=255)
                    cTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGtt                                                                                                                                <  2:1364889/149‑1 (MQ=255)
                    cTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGtt                                                                                                                                <  2:4358064/149‑1 (MQ=255)
                      gTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTc                                                                                                                              >  1:2211606/1‑149 (MQ=255)
                        aaCCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCt                                                                                                                            >  2:3973299/1‑149 (MQ=255)
                        aaCCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCt                                                                                                                            >  2:3589833/1‑149 (MQ=255)
                         aCCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTg                                                                                                                           <  1:1583944/149‑1 (MQ=255)
                          cccTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGNTAAATAACGCTATTACGTTTCCTGc                                                                                                                          >  1:1650114/1‑149 (MQ=255)
                            cgtGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTa                                                                                                                        <  2:1559438/147‑1 (MQ=255)
                               gCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAAcc                                                                                                                     <  2:4696929/149‑1 (MQ=255)
                                 gTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAt                                                                                                                   >  2:752525/1‑149 (MQ=255)
                                  tCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAta                                                                                                                  <  2:411221/149‑1 (MQ=255)
                                    tGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt                                                                                                                <  2:4041424/149‑1 (MQ=255)
                                     ggTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtg                                                                                                               >  1:1706865/1‑149 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgt                                                                                                              <  1:3113917/149‑1 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgt                                                                                                              >  1:1534509/1‑149 (MQ=255)
                                       tCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                             <  2:1877606/149‑1 (MQ=255)
                                       tCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                             <  2:4267807/149‑1 (MQ=255)
                                         gcggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagt                                                                                                           <  1:758792/149‑1 (MQ=255)
                                         gcggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagt                                                                                                           <  1:3657512/149‑1 (MQ=255)
                                         gcggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagt                                                                                                           <  1:4555287/149‑1 (MQ=255)
                                          cggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagtg                                                                                                          <  2:3641537/149‑1 (MQ=255)
                                               aaTCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta                                                                                                     >  2:304598/1‑149 (MQ=255)
                                                 tctcTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTACAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCttatt                                                                                                   >  2:4442609/1‑149 (MQ=255)
                                                         ggATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAAc                                                                                           <  1:3806385/149‑1 (MQ=255)
                                                         ggATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAAc                                                                                           <  1:156718/149‑1 (MQ=255)
                                                           aTGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACtt                                                                                         <  2:2459162/149‑1 (MQ=255)
                                                              gTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTgg                                                                                      <  1:2311722/149‑1 (MQ=255)
                                                              gTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTgg                                                                                      <  1:234900/149‑1 (MQ=255)
                                                               tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                     >  2:3189790/1‑149 (MQ=255)
                                                               tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                     <  2:2771967/149‑1 (MQ=255)
                                                               tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                     >  2:797942/1‑149 (MQ=255)
                                                               tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                     <  1:1053072/149‑1 (MQ=255)
                                                                   ttACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGaa                                                                                 >  2:3927045/1‑149 (MQ=255)
                                                                    tACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAAt                                                                                >  1:1870657/1‑149 (MQ=255)
                                                                     aCTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATg                                                                               <  1:902899/149‑1 (MQ=255)
                                                                      cTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGt                                                                              <  1:3393369/149‑1 (MQ=255)
                                                                      cTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGt                                                                              >  2:3511041/1‑149 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTCACCATAGTGTAGTGCCTGATTACTTTAACTTTGGGGGAATGTTcc                                                                           >  1:2157944/1‑149 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTcc                                                                           >  1:1760667/1‑149 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTcc                                                                           <  1:1157393/149‑1 (MQ=255)
                                                                            gTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGa                                                                        >  1:2100367/1‑149 (MQ=255)
                                                                            gTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACAACAACACCAATAATAAACTGGCAAACCGGTAAATAACGATATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGTGGAATGTTCCCGa                                                                        >  1:4499691/1‑149 (MQ=255)
                                                                             tGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGaa                                                                       <  1:3090629/149‑1 (MQ=255)
                                                                             tGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGaa                                                                       <  1:3090422/149‑1 (MQ=255)
                                                                                tggctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTg                                                                    <  2:2689249/149‑1 (MQ=255)
                                                                                tggctgTGGCGTACCTTGCAGTGCGCTACAGGCACATAACATCAACACCAATAATAAACTGGCACACCGGTAAATAACGATATAACGTTACCTGCTAACCAAACAGTAGTTCCATCTTAATATAACTATGGGGGACTGTTCCAGAAttt                                                                    >  1:4336411/1‑148 (MQ=255)
                                                                                 ggctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGt                                                                   <  2:880060/149‑1 (MQ=255)
                                                                                     gtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAcc                                                               <  2:2501421/149‑1 (MQ=255)
                                                                                        gCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTgg                                                            <  1:2403090/149‑1 (MQ=255)
                                                                                          gTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTa                                                          >  1:1028689/1‑149 (MQ=255)
                                                                                               ttGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCACACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGa                                                     >  2:4123630/1‑149 (MQ=255)
                                                                                                   aGTGCGCTACAGGCACTTAACATCTACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGAtttt                                                 <  2:1306713/149‑1 (MQ=255)
                                                                                                        gcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAAt                                            <  2:2737695/149‑1 (MQ=255)
                                                                                                         cTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATg                                           >  1:4739715/1‑149 (MQ=255)
                                                                                                             aGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGaaaa                                       >  1:2371054/1‑149 (MQ=255)
                                                                                                                  cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGa                                  >  2:1893534/1‑149 (MQ=255)
                                                                                                                  cTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGa                                  >  2:3726299/1‑149 (MQ=255)
                                                                                                                   ttAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGAc                                 <  1:3873555/149‑1 (MQ=255)
                                                                                                                     aaCATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGa                               >  1:1993344/1‑149 (MQ=255)
                                                                                                                        aTCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAg                            <  1:1398375/149‑1 (MQ=255)
                                                                                                                         tCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGc                           <  2:625231/149‑1 (MQ=255)
                                                                                                                           aaCACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGt                         <  1:4075125/149‑1 (MQ=255)
                                                                                                                            acacCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTa                        >  1:332937/1‑149 (MQ=255)
                                                                                                                            acacCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTa                        <  1:3981171/149‑1 (MQ=255)
                                                                                                                             cacCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTaa                       <  1:4388536/149‑1 (MQ=255)
                                                                                                                              acCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAc                      <  2:1823483/149‑1 (MQ=255)
                                                                                                                              acCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAc                      >  1:449659/1‑149 (MQ=255)
                                                                                                                                 aataatAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCca                   <  1:1832686/149‑1 (MQ=255)
                                                                                                                                   taataaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCcaca                 >  2:3081200/1‑149 (MQ=255)
                                                                                                                                    aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGAAGAAAGCGTAACCCACAt                >  2:1499975/1‑149 (MQ=255)
                                                                                                                                     ataaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtc               >  2:2539225/1‑149 (MQ=255)
                                                                                                                                       aaaCTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtctc             >  1:4591251/1‑149 (MQ=255)
                                                                                                                                        aaCTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCa            >  1:367829/1‑149 (MQ=255)
                                                                                                                                         aCTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCaa           <  1:109169/149‑1 (MQ=255)
                                                                                                                                         aCTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCaa           <  1:2961800/149‑1 (MQ=255)
                                                                                                                                          cTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAAc          <  2:934596/149‑1 (MQ=255)
                                                                                                                                             gCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAtt       <  2:3374072/149‑1 (MQ=255)
                                                                                                                                                aaCCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATg    <  1:820542/149‑1 (MQ=255)
                                                                                                                                                 aCCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGa   >  2:2268165/1‑149 (MQ=255)
                                                                                                                                                  ccGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGaa  <  1:4130938/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTGCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAA  >  CP009273/4406406‑4406700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑