BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,407,017	C→T	6.7%	I83I (ATC→ATT)	yjfP →	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,407,017	0	C	T	6.7%	1014.4 / 38.3	284	I83I (ATC→ATT)	yjfP	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base C (129/136); new base T (11/8); total (140/144)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

ACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAT  >  CP009273/4406869‑4407165

aCGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                                                                                                                                      <  1:1523371/149‑1 (MQ=255)

aCGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                                                                                                                                      >  2:191246/1‑149 (MQ=255)

 cGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCt                                                                                                                                                     <  1:818548/149‑1 (MQ=255)

  ggCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCGTGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCtt                                                                                                                                                    >  1:1833370/1‑149 (MQ=255)

  ggCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCtt                                                                                                                                                    >  1:1061984/1‑149 (MQ=255)

     tttACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCt                                                                                                                                                 >  1:2274125/1‑149 (MQ=255)

      ttACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTa                                                                                                                                                >  2:1180834/1‑149 (MQ=255)

         cTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGGTTTAGTGGTGACGCAGCGCGGCGGTTAAGTCAATTCTGGCAAATCTTGCTACaa                                                                                                                                             >  2:594513/1‑149 (MQ=255)

          ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                                                                                                                            <  1:1079055/149‑1 (MQ=255)

            cATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGt                                                                                                                                          >  1:1641576/1‑149 (MQ=255)

              tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTAt                                                                                                                                        >  1:2510310/1‑149 (MQ=255)

              tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTAt                                                                                                                                        >  1:4567598/1‑149 (MQ=255)

               ccAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATg                                                                                                                                       <  1:1894070/149‑1 (MQ=255)

                cAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGc                                                                                                                                      <  2:1728878/149‑1 (MQ=255)

                     tGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGaa                                                                                                                                 <  2:1244158/149‑1 (MQ=255)

                     tGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGaa                                                                                                                                 <  1:985169/149‑1 (MQ=255)

                     tGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGaa                                                                                                                                 >  1:698213/1‑149 (MQ=255)

                        tgtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTc                                                                                                                              <  2:4283927/149‑1 (MQ=255)

                         gtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                                                                                                                             >  2:424833/1‑149 (MQ=255)

                         gtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                                                                                                                             >  2:1157365/1‑149 (MQ=255)

                          tataGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCac                                                                                                                            >  1:553679/1‑149 (MQ=255)

                          tataGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCac                                                                                                                            <  1:1499987/149‑1 (MQ=255)

                            taGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCacta                                                                                                                          >  1:1470674/1‑149 (MQ=255)

                             aGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCactac                                                                                                                         >  1:4449754/1‑149 (MQ=255)

                             aGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCactac                                                                                                                         <  2:3206703/149‑1 (MQ=255)

                              gCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCactact                                                                                                                        >  1:1652566/1‑149 (MQ=255)

                              gCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCactact                                                                                                                        <  1:394637/149‑1 (MQ=255)

                               cTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACtt                                                                                                                       >  1:45540/1‑149 (MQ=255)

                                 aTTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTa                                                                                                                     <  2:3906489/149‑1 (MQ=255)

                                 aTTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTa                                                                                                                     <  1:2674895/149‑1 (MQ=255)

                                 aTTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTa                                                                                                                     <  2:4620005/149‑1 (MQ=255)

                                  ttttGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTAc                                                                                                                    >  1:1933974/1‑149 (MQ=255)

                                  ttttGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTAc                                                                                                                    >  1:3679712/1‑149 (MQ=255)

                                  ttttGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTAc                                                                                                                    >  2:4134648/1‑149 (MQ=255)

                                     tGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTg                                                                                                                 <  1:3773028/149‑1 (MQ=255)

                                      gCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGc                                                                                                                <  1:2041972/149‑1 (MQ=255)

                                          ttGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                            >  1:3401727/1‑149 (MQ=255)

                                            gcgcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAAt                                                                                                          <  2:2803603/149‑1 (MQ=255)

                                                    gcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                  <  2:4202539/149‑1 (MQ=255)

                                                      aGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaagaa                                                                                                >  2:2908126/1‑149 (MQ=255)

                                                       ggCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGaaa                                                                                               <  2:698213/149‑1 (MQ=255)

                                                           ggTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTg                                                                                           <  2:2274125/149‑1 (MQ=255)

                                                           ggTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTg                                                                                           >  1:657445/1‑149 (MQ=255)

                                                             tttGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgc                                                                                         >  1:3585665/1‑149 (MQ=255)

                                                             tttGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgc                                                                                         >  2:3437194/1‑149 (MQ=255)

                                                                 cGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctgct                                                                                     >  2:3782753/1‑149 (MQ=255)

                                                                    gTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtga                                                                                  >  2:2512855/1‑149 (MQ=255)

                                                                    gTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtga                                                                                  >  2:3629439/1‑149 (MQ=255)

                                                                        tCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAc                                                                              <  2:3816535/149‑1 (MQ=255)

                                                                           tGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGt                                                                           <  2:4200559/149‑1 (MQ=255)

                                                                            gCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTc                                                                          <  2:4050/149‑1 (MQ=255)

                                                                                 aTGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCa                                                                     >  1:100887/1‑149 (MQ=255)

                                                                                  tGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAg                                                                    <  2:4469269/149‑1 (MQ=255)

                                                                                    cgcCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTc                                                                  <  2:537461/149‑1 (MQ=255)

                                                                                        cGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTg                                                              <  1:2705441/149‑1 (MQ=255)

                                                                                         gATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTgg                                                             >  2:2945780/1‑149 (MQ=255)

                                                                                          aTCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                            >  2:3351276/1‑149 (MQ=255)

                                                                                          aTCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                            >  1:2637166/1‑149 (MQ=255)

                                                                                          aTCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGAAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                            >  1:3392176/1‑149 (MQ=255)

                                                                                           tCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgcg                                                           >  1:871827/1‑149 (MQ=255)

                                                                                           tCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgcg                                                           >  1:3019527/1‑149 (MQ=255)

                                                                                           tCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgcg                                                           >  2:3060016/1‑149 (MQ=255)

                                                                                           tCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgcg                                                           >  2:668938/1‑149 (MQ=255)

                                                                                              cGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAACTCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTGTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGt                                                        <  2:2555851/149‑1 (MQ=255)

                                                                                              cGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAGGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGt                                                        <  1:2387773/149‑1 (MQ=255)

                                                                                              cGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGt                                                        <  1:4030147/149‑1 (MQ=255)

                                                                                                     cGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATggg                                                 >  2:91643/1‑149 (MQ=255)

                                                                                                      gTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                                >  2:1563927/1‑149 (MQ=255)

                                                                                                       ttttAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgcg                                               >  2:353237/1‑149 (MQ=255)

                                                                                                          tAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGa                                            >  1:3200383/1‑149 (MQ=255)

                                                                                                           aGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGAt                                           <  1:2399330/149‑1 (MQ=255)

                                                                                                             tggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                         <  2:767417/149‑1 (MQ=255)

                                                                                                             tggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                         <  1:2089406/149‑1 (MQ=255)

                                                                                                             tggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                         <  1:2908126/149‑1 (MQ=255)

                                                                                                              ggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGAc                                        <  2:396549/149‑1 (MQ=255)

                                                                                                                     cAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTg                                 <  2:3726304/149‑1 (MQ=255)

                                                                                                                      aGCGCGGCGGTTAAATCAATTCTGGCAAATCTTTCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTgg                                >  1:2141204/1‑149 (MQ=255)

                                                                                                                          cggcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGAt                            >  2:2094796/1‑149 (MQ=255)

                                                                                                                           ggcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGAtt                           <  2:1033087/149‑1 (MQ=255)

                                                                                                                            gcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTa                          >  1:492971/1‑149 (MQ=255)

                                                                                                                             cggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTAc                         <  2:4549740/149‑1 (MQ=255)

                                                                                                                             cggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTAc                         <  2:1806244/149‑1 (MQ=255)

                                                                                                                               gTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCg                       >  1:4752344/1‑149 (MQ=255)

                                                                                                                               gTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCg                       >  1:1369759/1‑149 (MQ=255)

                                                                                                                                ttAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGc                      <  1:1157747/149‑1 (MQ=255)

                                                                                                                                 tAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCt                     <  2:1316493/149‑1 (MQ=255)

                                                                                                                                 tAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCt                     <  2:2457604/149‑1 (MQ=255)

                                                                                                                                     tCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGcc                 >  1:3830096/1‑149 (MQ=255)

                                                                                                                                         ttCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAccc             >  2:1421090/1‑149 (MQ=255)

                                                                                                                                         ttCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAccc             <  1:2630544/149‑1 (MQ=255)

                                                                                                                                          tCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAcccc            >  1:4411487/1‑149 (MQ=255)

                                                                                                                                           cTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCa           >  1:1015238/1‑149 (MQ=255)

                                                                                                                                               cAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGt       <  1:2423713/149‑1 (MQ=255)

                                                                                                                                               cAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGt       >  2:4699047/1‑149 (MQ=255)

                                                                                                                                               cAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGt       <  1:2804622/149‑1 (MQ=255)

                                                                                                                                                  aTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    >  1:3482683/1‑149 (MQ=255)

                                                                                                                                                  aTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    <  1:1177041/149‑1 (MQ=255)

                                                                                                                                                  aTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    >  1:789768/1‑149 (MQ=255)

                                                                                                                                                    cTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAt  <  2:158856/149‑1 (MQ=255)

                                                                                                                                                    cTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAt  <  2:2405035/149‑1 (MQ=255)

ACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAT  >  CP009273/4406869‑4407165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`