Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,411,145 T→G 9.2% L450R (CTG→CGG)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,411,1450TG 9.2% 548.2 / 8.0 282L450R (CTG→CGG) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base T (83/160);  new base G (29/5);  total (116/166)
Fisher's exact test for biased strand distribution p-value = 1.78e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAC  >  CP009273/4410997‑4411292
                                                                                                                                                    |                                                                                                                                                   
cTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCt                                                                                                                                                     >  2:4667331/1‑149 (MQ=255)
  cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                   >  2:852581/1‑149 (MQ=255)
  cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                   >  2:127172/1‑149 (MQ=255)
  cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                   <  1:3224338/149‑1 (MQ=255)
  cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTCGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                   <  1:808625/149‑1 (MQ=255)
          tGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaa                                                                                                                                           >  2:728185/1‑149 (MQ=255)
          tGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaa                                                                                                                                           >  1:1424209/1‑149 (MQ=255)
          tGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCAGCGCGCagaa                                                                                                                                           >  1:657986/1‑149 (MQ=255)
               gCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaaga                                                                                                                                      <  1:2832282/149‑1 (MQ=255)
                ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGAt                                                                                                                                     >  2:68326/1‑149 (MQ=255)
                      tGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGaa                                                                                                                               >  2:3791985/1‑149 (MQ=255)
                             ggCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                                                                                        <  2:1073174/149‑1 (MQ=255)
                             ggCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                                                                                        <  1:1205199/149‑1 (MQ=255)
                              gCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACt                                                                                                                       <  2:3957183/149‑1 (MQ=255)
                                ggACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                                                                                     <  2:3470795/149‑1 (MQ=255)
                                ggACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                                                                                     <  2:3678488/149‑1 (MQ=255)
                                ggACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACTgg                                                                                                                     >  2:585760/1‑149 (MQ=255)
                                 gACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGc                                                                                                                    <  1:2669047/149‑1 (MQ=255)
                                 gACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGc                                                                                                                    <  1:909432/149‑1 (MQ=255)
                                 gACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGc                                                                                                                    <  2:3520974/149‑1 (MQ=255)
                                      gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAAc                                                                                                               <  1:2534261/149‑1 (MQ=255)
                                      gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAAc                                                                                                               <  2:2012202/149‑1 (MQ=255)
                                      gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCGTATATGTTCTTCGCTGGCCGCGCGCAGCGCGCCCACCAAGTAGGACAAAAAAAACAGGCAGaaa                                                                                                               >  1:2163412/1‑148 (MQ=255)
                                         gATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                                                                            >  2:4319644/1‑149 (MQ=255)
                                         gATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                                                                            <  1:3433168/149‑1 (MQ=255)
                                             cTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGc                                                                                                        <  2:2275852/149‑1 (MQ=255)
                                              tGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCt                                                                                                       <  1:4608759/149‑1 (MQ=255)
                                              tGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCt                                                                                                       <  2:1431016/149‑1 (MQ=255)
                                               ggCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCtt                                                                                                      <  1:2240427/149‑1 (MQ=255)
                                               ggCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCtt                                                                                                      <  1:3140681/149‑1 (MQ=255)
                                                 cACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTtaa                                                                                                    <  1:4506696/149‑1 (MQ=255)
                                                  aCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTtaat                                                                                                   <  1:526467/149‑1 (MQ=255)
                                                         atgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGt                                                                                            <  2:3736006/149‑1 (MQ=255)
                                                            atgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtttt                                                                                         <  2:336924/149‑1 (MQ=255)
                                                            atgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtttt                                                                                         <  1:724078/149‑1 (MQ=255)
                                                              gCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                                                                                       <  1:2962572/149‑1 (MQ=255)
                                                                   gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGGGCGGCGCGCCGAAGAAGATGCAGAAAAAAAACAGGCAGAACAGTCTGCTTTATAAGGtgttttgtttttt                                                                                  >  1:3993382/1‑146 (MQ=255)
                                                                   gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                                                                                  <  2:3831250/149‑1 (MQ=255)
                                                                    cttcttCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATgt                                                                                 >  2:2202650/1‑148 (MQ=255)
                                                                    cttcttCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGa                                                                                 <  1:2117327/149‑1 (MQ=255)
                                                                    cttcttCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAACAGTCTGCTTAATAATGAGTTTTTATTATGa                                                                                 >  1:3274132/1‑149 (MQ=255)
                                                                     ttcttcTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                                                                <  2:1151098/149‑1 (MQ=255)
                                                                         tctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                                                                            <  1:4458511/149‑1 (MQ=255)
                                                                         tctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                                                                            <  1:2091189/149‑1 (MQ=255)
                                                                          ctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtac                                                                           <  1:1374067/149‑1 (MQ=255)
                                                                           tcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGAAAAAAAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGTCcgtaag                                                                          >  1:1505660/1‑147 (MQ=255)
                                                                           tcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacg                                                                          >  2:4353413/1‑149 (MQ=255)
                                                                            ccATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacgt                                                                         <  2:3856127/149‑1 (MQ=255)
                                                                               tCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCACAACAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTTATTATGACCGTACGTAtt                                                                      >  2:4589854/1‑149 (MQ=255)
                                                                                cGGTATCGCCTTTTTGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTc                                                                     <  2:1618057/149‑1 (MQ=255)
                                                                                cGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCTCTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTc                                                                     <  1:3085737/149‑1 (MQ=255)
                                                                                    aTCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGc                                                                 <  1:19859/149‑1 (MQ=255)
                                                                                     tCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGAAAAACAACTGGCAGTACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCt                                                                >  2:2590263/1‑149 (MQ=255)
                                                                                     tCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCt                                                                <  2:2855489/149‑1 (MQ=255)
                                                                                       gCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgt                                                              <  2:1387644/149‑1 (MQ=255)
                                                                                       gCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgt                                                              <  2:1673556/149‑1 (MQ=255)
                                                                                        ccTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtg                                                             <  2:4121906/149‑1 (MQ=255)
                                                                                          tttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtg                                                           <  2:3635381/149‑1 (MQ=255)
                                                                                           ttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtgt                                                          >  2:3551821/1‑149 (MQ=255)
                                                                                            tATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCAGCGCCCAGAATAAGATTCACAAAAAAAAAATGCATAACAAACAGCATAATAATGACTTTTGATTATTAACGTAAGAATTATGGCtgtgtgtt                                                         >  1:1826533/1‑148 (MQ=255)
                                                                                             aTGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTAGTCTGGCTGTGTGTgg                                                        <  1:3599370/149‑1 (MQ=255)
                                                                                              tGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCCGAAGAATATTCAGAAAAAAAAATGGCAGAAAAGTCTGCTTAATAATGAGTTTTGATTATGACCGTACGTATTATGGCTGTGTGTGGc                                                       >  1:1073081/1‑149 (MQ=255)
                                                                                               ggCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTTATTATGAACGTACGTATTCTGGCTGTTTGTGGCa                                                      >  2:1490075/1‑149 (MQ=255)
                                                                                                gCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCaa                                                     <  2:2373502/149‑1 (MQ=255)
                                                                                                 ccGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAACAGTCTGGTTTATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAAc                                                    >  2:822562/1‑149 (MQ=255)
                                                                                                   gTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACgg                                                  <  2:4222219/149‑1 (MQ=255)
                                                                                                    tcatcaTTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGAAAAAAAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATTACCGTACGTATTATGGCTGTTTGTGGCAACCGa                                                 >  1:2306692/1‑149 (MQ=255)
                                                                                                       tcatTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACaa                                              <  2:3291373/149‑1 (MQ=255)
                                                                                                          ttGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAACACTCTGCTTAATAATGAGTTTTTTTTATTACCGTACGGATTATGGCTGTGTGTGGCCACGGACAAAGc                                           >  1:4124229/1‑149 (MQ=255)
                                                                                                               aTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCCGAAGAAGATGCAGAAAAAAAACTGGCAGAAAAGTCTGCTTAATAAGGAGTTTTGTTTATGACCGTACGTATTATGGCTGTGTGTGGCAACGGGCAAGGCAGTTc                                      >  2:10436/1‑149 (MQ=255)
                                                                                                                ttGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTcc                                     >  2:3153902/1‑149 (MQ=255)
                                                                                                                 tGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCa                                    <  1:590816/149‑1 (MQ=255)
                                                                                                                  gCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGGTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCAt                                   >  2:1330890/1‑149 (MQ=255)
                                                                                                                  gCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCATAATAATAATCATAAAAAAAACATGCAGAACATTCATCTTAAAAATGAGTTTTGATTATTACCGTACGTATTATTGCTGTGTGTTGCAACGGACAAGGCAGTTCCaa                                   >  1:1589947/1‑148 (MQ=255)
                                                                                                                    aCTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTATGGCTGTGTGTGGCAACGGACAAGGCAGTTccctta                                 >  1:1119472/1‑145 (MQ=255)
                                                                                                                    aCTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTATGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGa                                 >  2:3933611/1‑149 (MQ=255)
                                                                                                                    aCTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCCCGCAGAAGAAGATTCAGAAAAACAACAGGCAGAACAGTCTGCGTAATAAGGAGTTTTTATTATGACCCGACGTATTATGGCTGTGTGTGGCAACCGACACGGCCGATCCAAGa                                 >  1:1129994/1‑149 (MQ=255)
                                                                                                                      tGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATc                               <  1:3476406/149‑1 (MQ=255)
                                                                                                                      tGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATc                               <  2:4191669/149‑1 (MQ=255)
                                                                                                                          ttATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGa                           <  1:4670002/149‑1 (MQ=255)
                                                                                                                           tataTGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGaa                          <  2:4416067/149‑1 (MQ=255)
                                                                                                                             tatGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGa                        <  1:1997043/149‑1 (MQ=255)
                                                                                                                             tatGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGa                        <  1:754299/149‑1 (MQ=255)
                                                                                                                             tatGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGa                        <  1:1763637/149‑1 (MQ=255)
                                                                                                                             tatGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAAAAGTCAGCTTAATAAGGAGTTTTGATTATGAACGTAAGTATTCTGGCTGTTTGTGGCAACCGACAAGGCAGTTCCATTATCATGAAGa                        >  1:4734379/1‑149 (MQ=255)
                                                                                                                              atGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGAt                       >  2:2978733/1‑149 (MQ=255)
                                                                                                                               tGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCACAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGAGCATGAAGATg                      >  2:3184626/1‑149 (MQ=255)
                                                                                                                                   cttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAg                  <  1:3846844/149‑1 (MQ=255)
                                                                                                                                     tcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTg                <  1:4269496/149‑1 (MQ=255)
                                                                                                                                        cTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGAc             <  1:3970050/149‑1 (MQ=255)
                                                                                                                                         tGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGAcc            <  2:471961/149‑1 (MQ=255)
                                                                                                                                           gCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAg          <  1:852581/149‑1 (MQ=255)
                                                                                                                                           gCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAg          <  1:127172/149‑1 (MQ=255)
                                                                                                                                            ccGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGt         <  1:4719592/149‑1 (MQ=255)
                                                                                                                                             cgcgcgCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGtt        <  1:1027270/149‑1 (MQ=255)
                                                                                                                                                  gcTGCGCGCAGAAGAAGATGTAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTaa   >  1:4313992/1‑149 (MQ=255)
                                                                                                                                                  gcTGCGCGCAGAAGAAGATGTAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTaa   >  1:1924412/1‑149 (MQ=255)
                                                                                                                                                   cTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAc  <  1:4638408/149‑1 (MQ=255)
                                                                                                                                                   cTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAc  <  2:1098803/149‑1 (MQ=255)
                                                                                                                                                   cTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAc  <  1:2901624/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAC  >  CP009273/4410997‑4411292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑