Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,413,724 C→A 5.5% R70S (CGC→AGC)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,413,7240CA 5.5% 1048.4 / 22.1 270R70S (CGC→AGC) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (133/122);  new base A (12/3);  total (145/125)
Fisher's exact test for biased strand distribution p-value = 5.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 272 total aligned reads displayed.

GACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTA  >  CP009273/4413579‑4413872
                                                                                                                                                 |                                                                                                                                                    
  ccTTTACCTGGGGCAACGTCCGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCACGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCa                                                                                                                                                 <  1:4571098/149‑1 (MQ=255)
  ccTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCa                                                                                                                                                 >  2:2645531/1‑149 (MQ=255)
    tttACCTGGGGAAACGTCAGCGCTATCGACCGCGAACGGGGGCTGGTGGTGATCAAGCCCAGCTGCGTTGCCTACTAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATc                                                                                                                                               <  1:1258371/149‑1 (MQ=255)
            gggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAACGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGaca                                                                                                                                       <  2:2680751/149‑1 (MQ=255)
            gggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGaca                                                                                                                                       <  2:1882385/149‑1 (MQ=255)
              gCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACt                                                                                                                                     >  2:1133104/1‑149 (MQ=255)
               cAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAACCCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTg                                                                                                                                    <  2:3911171/149‑1 (MQ=255)
               cAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTg                                                                                                                                    >  1:1102301/1‑149 (MQ=255)
               cAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTg                                                                                                                                    >  2:1060015/1‑149 (MQ=255)
               cAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTg                                                                                                                                    >  2:2004798/1‑149 (MQ=255)
               cAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTg                                                                                                                                    >  2:3448507/1‑149 (MQ=255)
                aaCGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGc                                                                                                                                   <  1:1906845/149‑1 (MQ=255)
                 aCGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCg                                                                                                                                  >  2:643726/1‑149 (MQ=255)
                 aCGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCg                                                                                                                                  >  2:2556926/1‑149 (MQ=255)
                        cgcTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCAtc                                                                                                                           <  2:3311369/149‑1 (MQ=255)
                          cTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAACCCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCAtctc                                                                                                                         <  2:599581/149‑1 (MQ=255)
                          cTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCAtctc                                                                                                                         >  1:3170298/1‑149 (MQ=255)
                           tATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCg                                                                                                                        >  2:3810418/1‑149 (MQ=255)
                            aTCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGa                                                                                                                       >  1:1994210/1‑149 (MQ=255)
                             tCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGaa                                                                                                                      >  2:3062773/1‑149 (MQ=255)
                                aCCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAActc                                                                                                                   <  1:2593395/149‑1 (MQ=255)
                                     gAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACcg                                                                                                              >  1:2279942/1‑149 (MQ=255)
                                            gggtggtggTGATCACGCCCTGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAc                                                                                                       <  1:819932/146‑1 (MQ=255)
                                            ggCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAc                                                                                                       <  1:2001033/149‑1 (MQ=255)
                                             gCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAcc                                                                                                      <  2:2420468/149‑1 (MQ=255)
                                              cTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCCTTAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAccc                                                                                                     <  1:4413060/149‑1 (MQ=255)
                                                 gtggtgATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTc                                                                                                  >  1:3942193/1‑149 (MQ=255)
                                                 gtggtgATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTAACCGTc                                                                                                  >  2:1520374/1‑149 (MQ=255)
                                                   ggtgATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGc                                                                                                <  2:356147/149‑1 (MQ=255)
                                                   ggtgATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGc                                                                                                <  2:1483565/149‑1 (MQ=255)
                                                        tCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTtggt                                                                                           <  1:4331593/149‑1 (MQ=255)
                                                          aaGCCCAGGGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTtggtgg                                                                                         <  1:1255847/149‑1 (MQ=255)
                                                           aGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCCTCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGGGtggtggg                                                                                        >  1:3317032/1‑148 (MQ=255)
                                                            gCCCAGCGGCGTTGCCTACGAAACCATGACAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCa                                                                                       <  2:2085157/149‑1 (MQ=255)
                                                             cccAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAt                                                                                      <  2:94347/149‑1 (MQ=255)
                                                             cccAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAt                                                                                      <  1:2227700/149‑1 (MQ=255)
                                                                tgcggcgTTGCCTACGAACCCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGt                                                                                   <  1:1724788/148‑1 (MQ=255)
                                                                 gcggcgTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCCTTGTc                                                                                  >  1:4539937/1‑149 (MQ=255)
                                                                  cggcgTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTcc                                                                                 <  2:3465072/149‑1 (MQ=255)
                                                                   ggcgTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCa                                                                                <  2:4088875/149‑1 (MQ=255)
                                                                    gcgTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCAt                                                                               >  1:382438/1‑149 (MQ=255)
                                                                          ccTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccac                                                                         <  1:4661543/149‑1 (MQ=255)
                                                                             ccGAAACATTTAACGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccactcc                                                                      <  1:2186690/148‑1 (MQ=255)
                                                                             aCGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccactcc                                                                      <  2:2896564/149‑1 (MQ=255)
                                                                               gAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACccactccac                                                                    >  2:4730696/1‑149 (MQ=255)
                                                                               gAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTACATACccactccac                                                                    >  2:869186/1‑149 (MQ=255)
                                                                               gAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTGGTGGCATTGTCCATACccactccac                                                                    >  2:3547847/1‑149 (MQ=255)
                                                                                aaaCCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCAAACccactccact                                                                   >  2:4588834/1‑149 (MQ=255)
                                                                                  aCCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCa                                                                 <  1:403434/149‑1 (MQ=255)
                                                                                    cATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACACATg                                                               >  2:2482977/1‑149 (MQ=255)
                                                                                     aTGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGc                                                              >  2:3575791/1‑149 (MQ=255)
                                                                                     aTGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGc                                                              <  2:1784621/149‑1 (MQ=255)
                                                                                        accgCGGCCGATATGGGGGTGGGCGCTCTGAGCGGCAAGGTGGGGGAAGGGGAGTCTCGCCCATCTTCCGACACTGCGACGCATCTCGCACTCTCCCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCAc                                                           <  2:2724203/146‑1 (MQ=255)
                                                                                        aaaGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCAc                                                           >  2:4380694/1‑149 (MQ=255)
                                                                                         aaGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCAcc                                                          >  2:1367285/1‑149 (MQ=255)
                                                                                         aaGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCAcc                                                          >  2:3973662/1‑149 (MQ=255)
                                                                                         aaGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGATGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAATTTCCAAAACCACTCCACTCAAGCCAcc                                                          >  1:482670/1‑149 (MQ=255)
                                                                                             ggCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACACCACAAAAGCCACCGCAt                                                      >  1:3975470/1‑149 (MQ=255)
                                                                                                 gATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATTCCACCGCATGGgc                                                  >  1:3906678/1‑149 (MQ=255)
                                                                                                    atGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCa                                               <  1:3550942/149‑1 (MQ=255)
                                                                                                     tggtggtggTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTTTACAAAACCAAACCACACAATCCACCCCAAGGGCGCAg                                              >  1:3653353/1‑149 (MQ=255)
                                                                                                     tggtggtggTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACACATGCCACCGCATGGGCGCAg                                              >  2:4443331/1‑149 (MQ=255)
                                                                                                     tggtggtggTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAg                                              >  2:4287317/1‑149 (MQ=255)
                                                                                                       gtggtggtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGc                                            <  1:2376802/149‑1 (MQ=255)
                                                                                                       gtggtggtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGc                                            <  1:380291/149‑1 (MQ=255)
                                                                                                           tggtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCgggg                                        <  2:1299626/149‑1 (MQ=255)
                                                                                                             gtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCt                                      <  2:510282/149‑1 (MQ=255)
                                                                                                             gtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAATGTACATACCCACAACCCGCATGCCCCCCCCAGGGGGCAGGCGGGGcg                                      >  1:928099/1‑148 (MQ=255)
                                                                                                              ttGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGGATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTg                                     >  2:2249174/1‑149 (MQ=255)
                                                                                                               tGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTgg                                    <  1:4632231/149‑1 (MQ=255)
                                                                                                                 atatGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACACCACACATGCCACCGCATGGGCGCAGGCGGGGCTGGCg                                  >  1:4480955/1‑149 (MQ=255)
                                                                                                                  tatGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTGGTGGGATTTTCCATAACCACAACCCACAAGCCACCCCATGGGCGCACGCGGggcgggcgc                                 >  1:837626/1‑148 (MQ=255)
                                                                                                                  tatGAGCGGCAAGGTGGTGGAAGGGGAGTATAGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAATGTACATACCCACACCACTCATTCCACCCCATGGGCGCAGGCGGGGCTGGCGa                                 >  1:3519209/1‑149 (MQ=255)
                                                                                                                     gAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTTTCCATACCCACACCACACATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATcc                              >  1:4742640/1‑149 (MQ=255)
                                                                                                                     gAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATcc                              <  2:2097375/149‑1 (MQ=255)
                                                                                                                     gAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTGGTGGCATTTTCCATACCCACACCACTCATTCCACCGCAAGGGCGCAGGCGGGGCTGGCGATcc                              >  1:276064/1‑149 (MQ=255)
                                                                                                                       gCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCg                            <  2:742089/149‑1 (MQ=255)
                                                                                                                       gCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCg                            <  2:1270864/149‑1 (MQ=255)
                                                                                                                       gCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCg                            <  2:4130765/149‑1 (MQ=255)
                                                                                                                       gCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACACATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATcccc                            >  2:2423696/1‑148 (MQ=255)
                                                                                                                         ggCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGc                          <  2:1213798/149‑1 (MQ=255)
                                                                                                                          gCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCg                         >  2:3862404/1‑149 (MQ=255)
                                                                                                                          gCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCg                         >  2:215883/1‑149 (MQ=255)
                                                                                                                           cAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCGTCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGt                        <  1:3103298/149‑1 (MQ=255)
                                                                                                                            aaGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGtt                       <  1:2474037/149‑1 (MQ=255)
                                                                                                                            aaGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGtt                       <  2:4653827/149‑1 (MQ=255)
                                                                                                                             aGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTTGTGGCATTGTCCAAAACCACAACACACATTCCACCCCAAGGGGGCAGGCGGGGCTGGCGATACCGGCGTTa                      >  1:4179882/1‑149 (MQ=255)
                                                                                                                             aGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCCCCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTa                      >  2:4090050/1‑149 (MQ=255)
                                                                                                                             aGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTa                      <  1:1480840/149‑1 (MQ=255)
                                                                                                                             aGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCAATTTGCATAACCACTACACGCATGCCACCGCCTGGGCGCAGGCGGGGCGGGCGATACCGGCGTTa                      >  1:2135341/1‑149 (MQ=255)
                                                                                                                              ggtggtGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTTGTGGCATTGTCCAAACCCACACCACACAATCCACCCCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGGTAg                     >  1:4708716/1‑149 (MQ=255)
                                                                                                                                     gAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGcaccac              <  1:2690357/149‑1 (MQ=255)
                                                                                                                                       aGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgc            <  2:2991433/149‑1 (MQ=255)
                                                                                                                                        ggggAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgca           <  1:2839982/149‑1 (MQ=255)
                                                                                                                                        ggggAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgca           <  2:211895/149‑1 (MQ=255)
                                                                                                                                        ggggAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgca           <  2:3379730/149‑1 (MQ=255)
                                                                                                                                        ggggAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTTGTTGCATTGTCCAAACCCACACCACACATTCCCCCGCATGGGCGCCGGCGGGGCTGGCGATCCCGGCGGTAGGCACcacgca           >  1:2884606/1‑149 (MQ=255)
                                                                                                                                               aTCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGGGGCATTGTCCATACCCACTCCACACATTCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGac    >  1:1005146/1‑149 (MQ=255)
                                                                                                                                                tCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGact   <  1:2658718/149‑1 (MQ=255)
                                                                                                                                                 cGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTTGTGGCATTGTCCATACCCACACCACTCATTCCACCGCATGGGCGCAGGCGGGGGTGGCGATCCCGGCGGTAGGCACCACGCACGCCGacta  >  1:1195662/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTA  >  CP009273/4413579‑4413872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑