BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,417,122	G→T	86.7%	R40S (CGT→AGT)	yjfZ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,417,122	0	G	T	86.7%	622.2 / 102.4	271	R40S (CGT→AGT)	yjfZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (15/21); new base T (99/134); total (116/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 272 total aligned reads displayed.

TTCCATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTT  >  CP009273/4416974‑4417265

ttCCATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTTAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGTTCTCTTCCACg                                                                                                                                                 >  1:2950919/1‑149 (MQ=255)

    atccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                             >  1:388283/1‑149 (MQ=255)

    atccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                             <  1:2409229/149‑1 (MQ=255)

    atccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGAttt                                                                                                                                             >  2:1241004/1‑149 (MQ=255)

     tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTg                                                                                                                                            >  2:3112820/1‑149 (MQ=255)

        aaaaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGaaa                                                                                                                                         >  1:1330869/1‑149 (MQ=255)

          aaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGt                                                                                                                                       >  2:744754/1‑149 (MQ=255)

          aaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGt                                                                                                                                       >  2:258586/1‑149 (MQ=255)

              aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATg                                                                                                                                   >  2:604673/1‑149 (MQ=255)

              aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATg                                                                                                                                   >  2:604213/1‑149 (MQ=255)

              aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATAGCAATTATGATCTCTTCCACTATTTGAAAGTAATg                                                                                                                                   >  1:954123/1‑149 (MQ=255)

                tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAGTTGAAAGTAATGTa                                                                                                                                 >  2:1800691/1‑149 (MQ=255)

                 aTCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAg                                                                                                                                <  1:4093217/149‑1 (MQ=255)

                 aTCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAg                                                                                                                                <  1:1847680/149‑1 (MQ=255)

                  tCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAgc                                                                                                                               <  1:121347/149‑1 (MQ=255)

                    aCCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAgcgc                                                                                                                             <  1:657830/149‑1 (MQ=255)

                     ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                            >  2:2064084/1‑149 (MQ=255)

                     ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                            <  1:3790916/149‑1 (MQ=255)

                     ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATATCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                            >  2:3891370/1‑149 (MQ=255)

                       attcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtg                                                                                                                          >  2:3150942/1‑149 (MQ=255)

                            ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTAt                                                                                                                     <  1:2213472/149‑1 (MQ=255)

                            ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTAt                                                                                                                     <  1:2561439/149‑1 (MQ=255)

                                 aTTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATTCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCAACGCAAATATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtgatg                                                                                                                >  1:196995/1‑149 (MQ=255)

                                 aTTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAAGGTAGCGCGTGCGTATtgatg                                                                                                                >  2:3172542/1‑149 (MQ=255)

                                     cATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaat                                                                                                            <  2:3692980/149‑1 (MQ=255)

                                          tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaa                                                                                                       >  1:2787623/1‑149 (MQ=255)

                                               aTTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAattttaatttta                                                                                                  <  1:3999171/149‑1 (MQ=255)

                                                ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTAc                                                                                                 <  2:1503977/149‑1 (MQ=255)

                                                 tACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACt                                                                                                >  2:2648826/1‑149 (MQ=255)

                                                 tACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACt                                                                                                <  2:2382552/149‑1 (MQ=255)

                                                 tACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACt                                                                                                <  2:3252039/149‑1 (MQ=255)

                                                 tACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACt                                                                                                <  2:87800/149‑1 (MQ=255)

                                                      cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGttt                                                                                           <  1:1537101/149‑1 (MQ=255)

                                                        cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                         <  1:1084810/149‑1 (MQ=255)

                                                        cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGt                                                                                         <  1:3714820/149‑1 (MQ=255)

                                                         gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTc                                                                                        >  1:3260678/1‑149 (MQ=255)

                                                         gtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTc                                                                                        <  2:2831986/149‑1 (MQ=255)

                                                          tcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCg                                                                                       >  2:2126149/1‑149 (MQ=255)

                                                             cTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTg                                                                                    <  2:1983448/149‑1 (MQ=255)

                                                              tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                   >  2:3337754/1‑149 (MQ=255)

                                                              tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                   >  1:303886/1‑149 (MQ=255)

                                                                  ttATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGa                                                                               <  1:4457239/149‑1 (MQ=255)

                                                                    aTTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGaaa                                                                             <  2:4674029/149‑1 (MQ=255)

                                                                      tGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTATCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGaaaaa                                                                           <  2:4414108/149‑1 (MQ=255)

                                                                        aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACg                                                                         >  1:349999/1‑149 (MQ=255)

                                                                         aTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATTAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACga                                                                        <  1:105817/149‑1 (MQ=255)

                                                                          tGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                       <  2:4236791/149‑1 (MQ=255)

                                                                           gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                      <  1:744754/149‑1 (MQ=255)

                                                                           gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                      <  1:2501344/149‑1 (MQ=255)

                                                                                ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                 >  2:1447161/1‑149 (MQ=255)

                                                                                ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                 <  2:1036626/149‑1 (MQ=255)

                                                                                 cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                                <  1:1815649/149‑1 (MQ=255)

                                                                                 cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                                <  1:4206743/149‑1 (MQ=255)

                                                                                  aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAatat                                                               <  1:360370/149‑1 (MQ=255)

                                                                                   gTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATg                                                              >  1:2606358/1‑149 (MQ=255)

                                                                                      tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAg                                                           <  2:4582158/149‑1 (MQ=255)

                                                                                       gagagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAgg                                                          >  2:827291/1‑149 (MQ=255)

                                                                                       gagagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAgg                                                          <  1:2847884/149‑1 (MQ=255)

                                                                                           gaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAAt                                                      <  1:817807/149‑1 (MQ=255)

                                                                                            aTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATg                                                     >  1:1190832/1‑149 (MQ=255)

                                                                                              cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaa                                                   >  1:706905/1‑149 (MQ=255)

                                                                                               tAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaat                                                  >  2:4120131/1‑149 (MQ=255)

                                                                                                    tgtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGactaaata                                             >  2:4322511/1‑149 (MQ=255)

                                                                                                    tgtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                             >  2:1931641/1‑149 (MQ=255)

                                                                                                    tgtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                             >  2:2456381/1‑149 (MQ=255)

                                                                                                        gATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCg                                         >  1:4284036/1‑149 (MQ=255)

                                                                                                        gATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCg                                         <  1:4604348/149‑1 (MQ=255)

                                                                                                          tctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGtt                                       <  1:4340264/149‑1 (MQ=255)

                                                                                                           ctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTg                                      >  2:664829/1‑149 (MQ=255)

                                                                                                            tcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTgg                                     <  1:868109/149‑1 (MQ=255)

                                                                                                              aaCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                   <  1:542156/149‑1 (MQ=255)

                                                                                                              aaCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                   <  1:3337754/149‑1 (MQ=255)

                                                                                                               aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTaa                                  <  1:2897852/149‑1 (MQ=255)

                                                                                                                 aGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATg                                >  2:1625134/1‑149 (MQ=255)

                                                                                                                  gTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGt                               >  1:4668341/1‑149 (MQ=255)

                                                                                                                  gTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGt                               >  1:3262065/1‑149 (MQ=255)

                                                                                                                      gTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCAtt                           <  1:2426829/149‑1 (MQ=255)

                                                                                                                       tCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATTAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTg                          >  2:375088/1‑149 (MQ=255)

                                                                                                                       tCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTg                          <  2:1184916/149‑1 (MQ=255)

                                                                                                                         tGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTa                        >  1:2522647/1‑149 (MQ=255)

                                                                                                                          gCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTaa                       <  2:3788272/149‑1 (MQ=255)

                                                                                                                            cATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                     <  2:783042/149‑1 (MQ=255)

                                                                                                                             atagCAATTATGATCTATTCCACTATATTAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGt                    <  2:2275263/146‑1 (MQ=255)

                                                                                                                              tCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                   <  1:3435213/149‑1 (MQ=255)

                                                                                                                               cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                  <  2:2917963/149‑1 (MQ=255)

                                                                                                                               cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                  <  2:118014/149‑1 (MQ=255)

                                                                                                                                gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                 <  1:1490021/149‑1 (MQ=255)

                                                                                                                                gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                 >  2:1567650/1‑149 (MQ=255)

                                                                                                                                   aTTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTg              >  1:1557749/1‑149 (MQ=255)

                                                                                                                                    ttATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt             <  2:4054893/149‑1 (MQ=255)

                                                                                                                                    ttATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt             <  1:2516931/149‑1 (MQ=255)

                                                                                                                                    ttATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt             >  1:1374379/1‑149 (MQ=255)

                                                                                                                                    ttATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt             <  2:1203283/149‑1 (MQ=255)

                                                                                                                                      aTGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGttt           <  1:2134285/149‑1 (MQ=255)

                                                                                                                                       tGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTa          >  2:268943/1‑149 (MQ=255)

                                                                                                                                        gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTaa         <  1:3111628/149‑1 (MQ=255)

                                                                                                                                        gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTaa         <  1:3242146/149‑1 (MQ=255)

                                                                                                                                          tctcTTCCACTTTTTGAAAGTTTTGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGTTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTTATg       <  2:4780855/149‑1 (MQ=255)

                                                                                                                                          tctcTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATg       <  2:2216209/149‑1 (MQ=255)

                                                                                                                                             ctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGttt    >  2:3464675/1‑149 (MQ=255)

TTCCATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTT  >  CP009273/4416974‑4417265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`