Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,609 G→T 100% D390Y (GAT→TAT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,6090GT99.6% 892.6 / ‑4.1 261D390Y (GAT→TAT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (134/124);  total (136/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

TGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCA  >  CP009273/4458463‑4458754
                                                                                                                                                  |                                                                                                                                                 
tGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTat                                                                                                                                                 >  1:1288083/1‑149 (MQ=255)
 ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                >  1:635941/1‑149 (MQ=255)
 ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                >  2:4140915/1‑149 (MQ=255)
 ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                >  2:1795383/1‑149 (MQ=255)
       cggcgCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCg                                                                                                                                          <  1:2265691/149‑1 (MQ=255)
          cgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTa                                                                                                                                       <  1:3545951/149‑1 (MQ=255)
           gcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaa                                                                                                                                      <  2:1825753/149‑1 (MQ=255)
            cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaa                                                                                                                                     <  2:4281076/149‑1 (MQ=255)
             aGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaaa                                                                                                                                    >  1:3513598/1‑149 (MQ=255)
              gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAc                                                                                                                                   <  2:564888/149‑1 (MQ=255)
              gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAc                                                                                                                                   >  2:2618871/1‑149 (MQ=255)
              gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAc                                                                                                                                   <  1:4688295/149‑1 (MQ=255)
               tAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAAcc                                                                                                                                  <  2:2434312/149‑1 (MQ=255)
               tAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAAcc                                                                                                                                  >  1:2381765/1‑149 (MQ=255)
                aaaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCa                                                                                                                                 >  2:3441961/1‑149 (MQ=255)
                  aGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGc                                                                                                                               >  2:1103228/1‑149 (MQ=255)
                     tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGaa                                                                                                                            <  2:4403444/149‑1 (MQ=255)
                      gTCTAAAAAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGAAATGGATATTCTGAGCAATGATAAAACCTGCACCCTGACGCAGGATAAAATAGTGCTGGAGAATAATACCTATAACTCCGGTAAAACCAGCGaaa                                                                                                                           >  2:2373156/1‑148 (MQ=255)
                      gTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAc                                                                                                                           >  1:1026507/1‑149 (MQ=255)
                      gTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAc                                                                                                                           >  2:3486165/1‑149 (MQ=255)
                            aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                     <  2:1453704/149‑1 (MQ=255)
                            aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                     <  2:2983659/149‑1 (MQ=255)
                            aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                     <  1:3686014/149‑1 (MQ=255)
                               gAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctgc                                                                                                                  >  1:2500195/1‑149 (MQ=255)
                               gAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctgc                                                                                                                  >  1:1060416/1‑149 (MQ=255)
                                 aaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCAt                                                                                                                <  1:3994351/149‑1 (MQ=255)
                                      aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAgcgc                                                                                                           >  2:3758815/1‑149 (MQ=255)
                                         gTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTg                                                                                                        <  1:979202/149‑1 (MQ=255)
                                          tCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTgg                                                                                                       >  1:3941533/1‑149 (MQ=255)
                                           cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGt                                                                                                      >  1:2828286/1‑149 (MQ=255)
                                            aaaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                     >  1:3339597/1‑149 (MQ=255)
                                            aaaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                     <  1:1056713/149‑1 (MQ=255)
                                             aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                    >  1:3825628/1‑149 (MQ=255)
                                             aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                    >  1:4126437/1‑149 (MQ=255)
                                             aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                    >  2:677462/1‑149 (MQ=255)
                                                 tCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACa                                                                                                >  1:2936820/1‑149 (MQ=255)
                                                  cTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAg                                                                                               <  2:817845/149‑1 (MQ=255)
                                                    ggATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTc                                                                                             >  1:3214394/1‑149 (MQ=255)
                                                    ggATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTc                                                                                             <  1:1411421/149‑1 (MQ=255)
                                                    ggATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTc                                                                                             >  2:1655580/1‑149 (MQ=255)
                                                    ggATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTc                                                                                             <  1:3800504/149‑1 (MQ=255)
                                                     gATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCa                                                                                            >  2:848764/1‑149 (MQ=255)
                                                      aTGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAt                                                                                           <  2:1951063/149‑1 (MQ=255)
                                                      aTGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAt                                                                                           <  2:4340624/149‑1 (MQ=255)
                                                       tGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAtt                                                                                          >  1:4439890/1‑149 (MQ=255)
                                                       tGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAtt                                                                                          >  1:49890/1‑149 (MQ=255)
                                                         cTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTAt                                                                                        >  1:1597604/1‑149 (MQ=255)
                                                              cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGAc                                                                                   <  2:1520161/149‑1 (MQ=255)
                                                                  aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                               >  2:912089/1‑149 (MQ=255)
                                                                  aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                               <  1:3759727/149‑1 (MQ=255)
                                                                  aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                               <  1:4325310/149‑1 (MQ=255)
                                                                       ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaa                                                                          >  1:4217947/1‑149 (MQ=255)
                                                                       ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaa                                                                          >  2:780075/1‑149 (MQ=255)
                                                                          gcAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaaaaa                                                                       <  2:1297780/149‑1 (MQ=255)
                                                                           cAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAc                                                                      >  2:3986846/1‑149 (MQ=255)
                                                                           cAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAc                                                                      >  2:3527669/1‑149 (MQ=255)
                                                                            aaTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATCGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAcc                                                                     >  2:3291171/1‑149 (MQ=255)
                                                                             aTGGATATTCTGTGCACTGGTAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                    <  1:2719668/149‑1 (MQ=255)
                                                                             aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                    >  2:3202143/1‑149 (MQ=255)
                                                                             aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                    >  2:2733154/1‑149 (MQ=255)
                                                                             aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                    <  1:462013/149‑1 (MQ=255)
                                                                             aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                    >  1:303630/1‑149 (MQ=255)
                                                                               ggATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGc                                                                  <  2:3378023/149‑1 (MQ=255)
                                                                                 atatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTc                                                                >  2:2389145/1‑149 (MQ=255)
                                                                                  tatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCg                                                               >  2:4589283/1‑149 (MQ=255)
                                                                                    ttCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGAt                                                             >  1:2984169/1‑149 (MQ=255)
                                                                                      cTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTTGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATAc                                                           <  1:173586/149‑1 (MQ=255)
                                                                                      cTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATAc                                                           >  1:2531663/1‑149 (MQ=255)
                                                                                       tgtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACa                                                          >  2:2044025/1‑149 (MQ=255)
                                                                                        gtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                         >  2:4067654/1‑149 (MQ=255)
                                                                                         ttcACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATAACTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGc                                                        >  2:2235181/3‑149 (MQ=255)
                                                                                         tgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGc                                                        <  2:3645760/149‑1 (MQ=255)
                                                                                           cACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCgg                                                      >  1:1773799/1‑149 (MQ=255)
                                                                                              tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                   >  1:1114585/1‑149 (MQ=255)
                                                                                               gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                  <  1:371553/149‑1 (MQ=255)
                                                                                               gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                  >  2:2135315/1‑149 (MQ=255)
                                                                                               gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                  >  2:1225827/1‑149 (MQ=255)
                                                                                                 tAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCg                                                >  2:4449283/1‑149 (MQ=255)
                                                                                                 tAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCg                                                >  1:313068/1‑149 (MQ=255)
                                                                                                  aaaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGa                                               >  2:2290550/1‑149 (MQ=255)
                                                                                                    aaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAg                                             >  1:5340/1‑149 (MQ=255)
                                                                                                      ccGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGt                                           >  1:2457859/1‑149 (MQ=255)
                                                                                                          cACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATACCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGACGGTACgg                                       <  1:4790941/149‑1 (MQ=255)
                                                                                                           aCCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGa                                      <  2:2910501/149‑1 (MQ=255)
                                                                                                           aCCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGa                                      <  2:1026772/149‑1 (MQ=255)
                                                                                                              cTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGa                                   >  1:1481385/1‑149 (MQ=255)
                                                                                                                     aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                            >  1:1891969/1‑149 (MQ=255)
                                                                                                                       gATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                          >  1:2900856/1‑149 (MQ=255)
                                                                                                                       gATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                          <  2:711452/149‑1 (MQ=255)
                                                                                                                          aaaaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcg                       >  1:4531604/1‑149 (MQ=255)
                                                                                                                            aaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                     >  2:3054838/1‑149 (MQ=255)
                                                                                                                            aaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                     <  2:2112023/149‑1 (MQ=255)
                                                                                                                            aaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                     <  1:4726969/149‑1 (MQ=255)
                                                                                                                              ttGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcg                   >  2:3930041/1‑149 (MQ=255)
                                                                                                                                  gCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTgg               >  2:4670001/1‑149 (MQ=255)
                                                                                                                                    tGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGcc             <  2:1060416/149‑1 (MQ=255)
                                                                                                                                      gagaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAg           <  1:862313/149‑1 (MQ=255)
                                                                                                                                        gaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTc         >  1:2649344/1‑149 (MQ=255)
                                                                                                                                          aTCATACCTATATCTCCTGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGt       <  2:2839577/149‑1 (MQ=255)
                                                                                                                                               aCCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCa  >  1:1088978/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCA  >  CP009273/4458463‑4458754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑