BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,466,947	A→T	7.9%	Q565L (CAA→CTA)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,466,947	0	A	T	7.9%	731.3 / 41.3	229	Q565L (CAA→CTA)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (122/89); new base T (15/3); total (137/92)
Fisher's exact test for biased strand distribution p-value = 4.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTA  >  CP009273/4466800‑4467095

tttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCaa                                                                                                                                                     <  1:4195000/149‑1 (MQ=255)

       tGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGagag                                                                                                                                              >  1:2332100/1‑149 (MQ=255)

           aaTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCTATCGAGAGTTaa                                                                                                                                          >  1:414242/1‑149 (MQ=255)

           aaTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTaa                                                                                                                                          >  1:3708836/1‑149 (MQ=255)

            aTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAAt                                                                                                                                         <  1:3650737/149‑1 (MQ=255)

              ttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                                       <  2:1978185/149‑1 (MQ=255)

                aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTAt                                                                                                                                     >  1:698655/1‑149 (MQ=255)

                    tatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAcc                                                                                                                                 >  2:4368115/1‑149 (MQ=255)

                        aTTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                             <  2:2780115/149‑1 (MQ=255)

                         tttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatata                                                                                                                            <  2:4025048/149‑1 (MQ=255)

                             aatgataatgatAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTaa                                                                                                                        >  1:4049902/1‑149 (MQ=255)

                             aatgataatgatAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTaa                                                                                                                        >  2:4213263/1‑149 (MQ=255)

                                  taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAAc                                                                                                                   >  1:1120016/1‑149 (MQ=255)

                                     tgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGc                                                                                                                >  2:461206/1‑149 (MQ=255)

                                       ataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGt                                                                                                              >  2:289481/1‑149 (MQ=255)

                                         aaGGAGTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGttt                                                                                                            >  1:737918/1‑149 (MQ=255)

                                          aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTta                                                                                                           >  1:1884866/1‑149 (MQ=255)

                                          aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTta                                                                                                           <  1:2164158/149‑1 (MQ=255)

                                            gAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaa                                                                                                         >  1:2716731/1‑149 (MQ=255)

                                             aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaat                                                                                                        >  1:4488291/1‑149 (MQ=255)

                                              aTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaata                                                                                                       >  1:3659731/1‑149 (MQ=255)

                                                 taataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAAc                                                                                                    >  2:1892494/1‑149 (MQ=255)

                                                 taataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAAc                                                                                                    >  1:842992/1‑149 (MQ=255)

                                                    taaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaa                                                                                                 <  1:2432324/149‑1 (MQ=255)

                                                       aGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATc                                                                                              <  1:1058344/149‑1 (MQ=255)

                                                        gAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATcc                                                                                             <  1:1828492/149‑1 (MQ=255)

                                                           aTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                          >  1:3111180/1‑149 (MQ=255)

                                                           aTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                          >  2:4088698/1‑149 (MQ=255)

                                                           aTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                          <  1:4340621/149‑1 (MQ=255)

                                                           aTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                          >  2:3606418/1‑149 (MQ=255)

                                                                gaagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGaaa                                                                                     >  1:1259466/1‑149 (MQ=255)

                                                                 aagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGaaaa                                                                                    >  1:541096/1‑149 (MQ=255)

                                                                  agaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAt                                                                                   >  1:3396868/1‑149 (MQ=255)

                                                                   gaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAtt                                                                                  <  1:3235416/149‑1 (MQ=255)

                                                                    aaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAttt                                                                                 <  2:3371535/149‑1 (MQ=255)

                                                                     aTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGACTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTa                                                                                <  2:3964172/149‑1 (MQ=255)

                                                                     aTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTa                                                                                <  2:1212147/149‑1 (MQ=255)

                                                                     aTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTa                                                                                <  1:207796/149‑1 (MQ=255)

                                                                      tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTAc                                                                               >  2:3341267/1‑149 (MQ=255)

                                                                       cAACCAGATAATCTAATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACg                                                                              <  2:4688819/149‑1 (MQ=255)

                                                                         aCCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGta                                                                            >  2:33804/1‑149 (MQ=255)

                                                                         aCCAGATAATGTTATCAAAGAAAAACTGAAGAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGta                                                                            <  2:2511418/149‑1 (MQ=255)

                                                                          ccAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtat                                                                           <  2:1591901/149‑1 (MQ=255)

                                                                            aGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAg                                                                         <  2:3166325/149‑1 (MQ=255)

                                                                             gATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGc                                                                        >  2:3791932/1‑149 (MQ=255)

                                                                             gATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGc                                                                        >  2:624512/1‑149 (MQ=255)

                                                                             gATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGc                                                                        <  1:2242529/149‑1 (MQ=255)

                                                                              aTAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCa                                                                       >  1:3193228/1‑149 (MQ=255)

                                                                               tAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCaa                                                                      <  1:3854835/149‑1 (MQ=255)

                                                                                aaTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                                     <  2:1997495/149‑1 (MQ=255)

                                                                                aaTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                                     <  2:3429440/149‑1 (MQ=255)

                                                                                aaTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                                     <  1:1326988/149‑1 (MQ=255)

                                                                                 aTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATa                                                                    >  2:574536/1‑149 (MQ=255)

                                                                                 aTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATa                                                                    >  2:2266762/1‑149 (MQ=255)

                                                                                   gTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGa                                                                  <  1:3660096/149‑1 (MQ=255)

                                                                                       tCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAacac                                                              <  1:3494398/149‑1 (MQ=255)

                                                                                        cAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACt                                                             <  1:3502148/149‑1 (MQ=255)

                                                                                         aaaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGACAATTTACGTATAGCAATAGAACACtt                                                            >  2:2195537/1‑149 (MQ=255)

                                                                                           aGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTg                                                          <  2:2624358/149‑1 (MQ=255)

                                                                                           aGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTg                                                          >  2:27134/1‑149 (MQ=255)

                                                                                                aaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTgg                                                     >  1:1464798/1‑149 (MQ=255)

                                                                                                 aCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGga                                                    <  2:2647961/149‑1 (MQ=255)

                                                                                                  cTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaa                                                   >  2:1685754/1‑149 (MQ=255)

                                                                                                  cTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaa                                                   <  1:2788605/149‑1 (MQ=255)

                                                                                                  cTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaa                                                   >  2:3031028/1‑149 (MQ=255)

                                                                                                   tGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCACTAGAACACTTTGGGTGgaag                                                  >  2:2967702/1‑149 (MQ=255)

                                                                                                    gAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaaga                                                 >  2:1401572/1‑149 (MQ=255)

                                                                                                    gAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaaga                                                 >  1:2052284/1‑149 (MQ=255)

                                                                                                    gAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaaga                                                 >  1:2563939/1‑149 (MQ=255)

                                                                                                        aatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAg                                             >  1:341216/1‑149 (MQ=255)

                                                                                                        aatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAg                                             >  1:1450903/1‑149 (MQ=255)

                                                                                                        aatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAg                                             <  2:564416/149‑1 (MQ=255)

                                                                                                          tGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGAc                                           >  1:1440804/1‑149 (MQ=255)

                                                                                                            aaTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCt                                         >  2:1325488/1‑149 (MQ=255)

                                                                                                              tACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTAt                                       >  1:1608451/1‑149 (MQ=255)

                                                                                                                 aaaCTTAGATTTAGAATGATGCAAACTATCTTGCTATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCAc                                    >  2:2954029/1‑149 (MQ=255)

                                                                                                                  aaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACt                                   >  1:1991244/1‑149 (MQ=255)

                                                                                                                   aCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTg                                  >  2:1206812/1‑149 (MQ=255)

                                                                                                                      tAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAt                               <  2:1156806/149‑1 (MQ=255)

                                                                                                                        gATTTAGAATGATGCAAACTATCTTGCTATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaa                             >  2:3612363/1‑149 (MQ=255)

                                                                                                                        gATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaa                             >  2:4337460/1‑149 (MQ=255)

                                                                                                                         aTTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaaa                            >  1:2703022/1‑149 (MQ=255)

                                                                                                                         aTTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaaa                            >  2:3145313/1‑149 (MQ=255)

                                                                                                                           ttAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaat                          >  1:3811562/1‑149 (MQ=255)

                                                                                                                             aGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTATGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaataa                        >  2:1314881/1‑149 (MQ=255)

                                                                                                                               aaTGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGACAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                      >  2:3948313/1‑149 (MQ=255)

                                                                                                                                atgatgCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                     >  1:438550/1‑149 (MQ=255)

                                                                                                                                    tgCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                 >  2:4707564/1‑149 (MQ=255)

                                                                                                                                     gCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGc                <  1:2392749/149‑1 (MQ=255)

                                                                                                                                       aaaCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg              >  1:1777839/1‑149 (MQ=255)

                                                                                                                                        aaCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCgg             >  2:1501152/1‑149 (MQ=255)

                                                                                                                                          cTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa           <  2:722578/149‑1 (MQ=255)

                                                                                                                                          cTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa           <  2:862637/149‑1 (MQ=255)

                                                                                                                                           tATCTTGCTATCGAGAGTTAATTTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAt          <  2:435901/149‑1 (MQ=255)

                                                                                                                                            aTCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATg         <  2:1415893/149‑1 (MQ=255)

                                                                                                                                               ttGCAATCTATATTTAATGTATTACCATATATTAATGAACAGAGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGtt      <  2:3719223/149‑1 (MQ=255)

                                                                                                                                               gtGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGtt      <  2:3688622/148‑1 (MQ=255)

                                                                                                                                                tGCTATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt     >  1:2419711/1‑149 (MQ=255)

                                                                                                                                                  cAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGt   <  1:1732261/149‑1 (MQ=255)

                                                                                                                                                  cAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGt   <  2:698655/149‑1 (MQ=255)

TTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTA  >  CP009273/4466800‑4467095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`