BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,468,165	A→G	100%	intergenic (‑37/‑125)	argI ← / → rraB	ornithine carbamoyltransferase 1/protein inhibitor of RNase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,468,165	0	A	G	99.7%	1248.8 / ‑4.9	306	intergenic (‑37/‑125)	argI/rraB	ornithine carbamoyltransferase 1/protein inhibitor of RNase E
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (134/170); total (135/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 311 total aligned reads displayed.

TACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGAACAACTG  >  CP009273/4468017‑4468310

tACCGCTTTTCGTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTaaaa                                                                                                                                                   <  1:3150830/149‑1 (MQ=255)

    gCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCa                                                                                                                                               <  2:2749768/149‑1 (MQ=255)

    gCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCa                                                                                                                                               <  2:4638682/149‑1 (MQ=255)

     cTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCaa                                                                                                                                              <  2:1595277/149‑1 (MQ=255)

     cTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCaa                                                                                                                                              <  1:4059536/149‑1 (MQ=255)

      ttttCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAAt                                                                                                                                             <  1:3156215/149‑1 (MQ=255)

      ttttCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAAt                                                                                                                                             <  2:4592883/149‑1 (MQ=255)

       tttCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAAtt                                                                                                                                            <  2:4432402/149‑1 (MQ=255)

         tCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTta                                                                                                                                          >  2:3273020/1‑149 (MQ=255)

         tCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTta                                                                                                                                          >  1:4643502/1‑149 (MQ=255)

           ttATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTtata                                                                                                                                        >  1:970178/1‑149 (MQ=255)

               cGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGga                                                                                                                                    <  2:2273194/149‑1 (MQ=255)

               cGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGga                                                                                                                                    <  1:2865317/149‑1 (MQ=255)

                ggCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgat                                                                                                                                   <  2:1830495/149‑1 (MQ=255)

                 gCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgatg                                                                                                                                  >  2:3479330/1‑149 (MQ=255)

                 gCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgatg                                                                                                                                  <  1:3618354/149‑1 (MQ=255)

                 gCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgatg                                                                                                                                  <  1:1464110/149‑1 (MQ=255)

                  cTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgatga                                                                                                                                 <  2:1021674/149‑1 (MQ=255)

                  cTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGgatga                                                                                                                                 <  2:630617/149‑1 (MQ=255)

                     tCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGatta                                                                                                                              >  2:853842/1‑149 (MQ=255)

                      cAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGattat                                                                                                                             <  1:2197548/149‑1 (MQ=255)

                        gCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTc                                                                                                                           <  2:3234726/149‑1 (MQ=255)

                           tCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCAtt                                                                                                                        <  1:470482/149‑1 (MQ=255)

                            cgcgGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCAttt                                                                                                                       >  2:111172/1‑149 (MQ=255)

                                gCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCaa                                                                                                                   >  2:4766418/1‑149 (MQ=255)

                                gCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCaa                                                                                                                   <  2:1851857/149‑1 (MQ=255)

                                   aaCTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTc                                                                                                                >  2:3848133/1‑149 (MQ=255)

                                   aaCTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTc                                                                                                                <  1:1411518/149‑1 (MQ=255)

                                   aaCTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTc                                                                                                                >  2:1409596/1‑149 (MQ=255)

                                    aCTGCAGCAGGCTGTTGAGTTCAGCTGGAGTGAAATCGAGTAATTTCAGGAATTGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCt                                                                                                               <  1:4469322/149‑1 (MQ=255)

                                      tGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTaa                                                                                                             <  2:1837883/149‑1 (MQ=255)

                                           cagGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCAt                                                                                                        <  2:1912125/149‑1 (MQ=255)

                                             ggCTGTTGAGTTCAGCTCGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATaa                                                                                                      <  2:2344293/149‑1 (MQ=255)

                                                 gTTGAGTTCAGCTGGCGTGAAAGCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGTCTAACGCCTCAATGAAAAAAATTTCAATTTATATTGATGATTATTCATTTGCAAGACTAAAGAATAAATCt                                                                                                  >  2:1784577/1‑149 (MQ=255)

                                                     aGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGt                                                                                              <  2:4127846/149‑1 (MQ=255)

                                                      gTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTc                                                                                             >  1:2771810/1‑149 (MQ=255)

                                                      gTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTc                                                                                             <  2:1888894/149‑1 (MQ=255)

                                                        tCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTcac                                                                                           <  2:4692064/149‑1 (MQ=255)

                                                         cAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTcacc                                                                                          >  2:591327/1‑148 (MQ=255)

                                                             tGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg                                                                                      <  1:2579712/149‑1 (MQ=255)

                                                             tGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg                                                                                      >  1:2538544/1‑149 (MQ=255)

                                                              ggCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGt                                                                                     <  1:829139/149‑1 (MQ=255)

                                                               gCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTg                                                                                    <  1:230577/149‑1 (MQ=255)

                                                                cGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTgg                                                                                   <  1:3932273/149‑1 (MQ=255)

                                                                   gAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAgg                                                                                >  1:4067555/1‑149 (MQ=255)

                                                                      aTCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa                                                                             >  1:4252004/1‑149 (MQ=255)

                                                                      aTCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa                                                                             >  1:2788390/1‑149 (MQ=255)

                                                                      aTCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa                                                                             >  1:1726460/1‑149 (MQ=255)

                                                                         gAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGt                                                                          >  1:543546/1‑149 (MQ=255)

                                                                           gTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCa                                                                        >  2:2465736/1‑149 (MQ=255)

                                                                            tAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAg                                                                       <  2:439946/149‑1 (MQ=255)

                                                                            tAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAg                                                                       >  2:4510073/1‑149 (MQ=255)

                                                                             actttCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCAAAAAGGGGGAGGCACTGTCAgt                                                                      >  2:485836/3‑149 (MQ=255)

                                                                              aTTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtg                                                                     >  2:1351307/1‑149 (MQ=255)

                                                                              aTTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtg                                                                     <  1:1641085/149‑1 (MQ=255)

                                                                               tttCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtgg                                                                    <  2:787843/149‑1 (MQ=255)

                                                                                  cAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGgtgt                                                                 >  2:808348/1‑149 (MQ=255)

                                                                                    ggAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGgtgtgt                                                               >  2:3689752/1‑149 (MQ=255)

                                                                                     gAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGtgtgtg                                                              >  1:738705/1‑149 (MQ=255)

                                                                                      aaaTGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa                                                             >  2:419866/1‑149 (MQ=255)

                                                                                       aaTACTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGAc                                                            <  2:2837383/149‑1 (MQ=255)

                                                                                            ttATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATaa                                                       <  1:1199453/149‑1 (MQ=255)

                                                                                             tATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAag                                                      >  1:2688509/1‑149 (MQ=255)

                                                                                             tATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAag                                                      <  2:2053267/149‑1 (MQ=255)

                                                                                               tGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag                                                    <  1:1691952/149‑1 (MQ=255)

                                                                                               tGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag                                                    >  2:2227197/1‑149 (MQ=255)

                                                                                                gATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGt                                                   <  1:2429009/149‑1 (MQ=255)

                                                                                                 aTAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTa                                                  >  1:3943940/1‑149 (MQ=255)

                                                                                                   aaaaCCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATc                                                >  1:2832115/1‑149 (MQ=255)

                                                                                                    aaaCCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACACTAAGAGTATCg                                               >  1:2008603/1‑149 (MQ=255)

                                                                                                      aCCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGc                                             >  1:1316935/1‑149 (MQ=255)

                                                                                                        ccGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAg                                           <  2:2379288/149‑1 (MQ=255)

                                                                                                        ccGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAg                                           >  1:2259743/1‑149 (MQ=255)

                                                                                                         cGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAgg                                          >  2:1490604/1‑149 (MQ=255)

                                                                                                          ggACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGCGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa                                         >  2:3621231/1‑149 (MQ=255)

                                                                                                          ggACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa                                         <  1:48375/149‑1 (MQ=255)

                                                                                                           gACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCACTGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGAc                                        >  2:4032612/1‑149 (MQ=255)

                                                                                                             cATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACAt                                      <  1:2511790/149‑1 (MQ=255)

                                                                                                              aTAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACAtt                                     <  1:2651010/149‑1 (MQ=255)

                                                                                                                 gATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAag                                  <  2:1969608/149‑1 (MQ=255)

                                                                                                                 gATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAag                                  >  1:3723986/1‑149 (MQ=255)

                                                                                                                  aTCCCTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAaga                                 >  2:2785760/1‑149 (MQ=255)

                                                                                                                  aTCACTCCTGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGAATATTCATATGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGAGGAGTGTGACAATAAGAGTATCGGCAGGACATTAaga                                 >  1:4322515/1‑149 (MQ=255)

                                                                                                                      ctcctGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGaa                             <  1:1004228/149‑1 (MQ=255)

                                                                                                                      ctcctGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGaa                             >  2:4000277/1‑149 (MQ=255)

                                                                                                                      ctcctGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGaa                             <  2:693578/149‑1 (MQ=255)

                                                                                                                        cctGTGGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATg                           <  2:4181098/149‑1 (MQ=255)

                                                                                                                           gtgGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGc                        <  1:4558995/149‑1 (MQ=255)

                                                                                                                           gtgGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGc                        <  1:2493262/149‑1 (MQ=255)

                                                                                                                           gtgGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGc                        <  1:3531439/149‑1 (MQ=255)

                                                                                                                            tgGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGcc                       <  2:4094576/149‑1 (MQ=255)

                                                                                                                            tgGCTAACGCCTCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGcc                       <  2:2673798/149‑1 (MQ=255)

                                                                                                                                  aCGCCTCAATGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCa                 <  1:3014055/149‑1 (MQ=255)

                                                                                                                                       tCAATGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAAccc            >  1:3325475/1‑149 (MQ=255)

                                                                                                                                         aaTGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCgg          >  2:30675/1‑149 (MQ=255)

                                                                                                                                          aTGAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGa         >  1:3762356/1‑149 (MQ=255)

                                                                                                                                            gAATTAAAGTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGaac       <  2:3018678/149‑1 (MQ=255)

                                                                                                                                              aTTAAAGTTCAATTTATATGGATGATTGTTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGaacaa     >  2:1547202/1‑149 (MQ=255)

                                                                                                                                                 aaagTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGAACAACTg  <  1:4446596/149‑1 (MQ=255)

                                                                                                                                                 aaagTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGAACAACTg  <  1:1128427/149‑1 (MQ=255)

TACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAATGCTTATGATAAAACCCGGACATAGATCCCTCCTGTGGCTAACGCCTCAATGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGCCATGGCAAACCCGGAACAACTG  >  CP009273/4468017‑4468310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`