Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,471,930 T→A 87.7% P575P (CCA→CCT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,471,9300TA87.7% 883.7 / 91.9 293P575P (CCA→CCT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (19/17);  new base A (136/121);  total (155/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

GGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGA  >  CP009273/4471783‑4472075
                                                                                                                                                   |                                                                                                                                                 
 gTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAgg                                                                                                                                                 >  1:301846/1‑149 (MQ=255)
   aGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCa                                                                                                                                               <  1:1155929/149‑1 (MQ=255)
   aGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCa                                                                                                                                               >  2:2068330/1‑149 (MQ=255)
    gACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                              >  2:3773842/1‑149 (MQ=255)
         cAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGaaa                                                                                                                                         <  1:216498/149‑1 (MQ=255)
          aGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAAt                                                                                                                                        >  2:862296/1‑149 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                       >  1:3904297/1‑149 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                       >  1:4762354/1‑149 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                       >  1:4762364/1‑149 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                       >  1:2178767/1‑149 (MQ=255)
           gcgcCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                       <  2:2849258/149‑1 (MQ=255)
              ccgccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCg                                                                                                                                    >  1:1833244/1‑149 (MQ=255)
               cgccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGt                                                                                                                                   <  2:2793327/149‑1 (MQ=255)
                gccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCAGCCAGCTGCGGCTGCATCATATTGCCGGTACGATTTTCCAGCAGTTCAGGCAGCGAAATACCGTc                                                                                                                                  >  2:406266/1‑149 (MQ=255)
                  cAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCaa                                                                                                                                >  1:3875169/1‑149 (MQ=255)
                    gggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAcc                                                                                                                              <  2:503644/149‑1 (MQ=255)
                      gTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCat                                                                                                                            >  2:1964865/1‑149 (MQ=255)
                      gTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCat                                                                                                                            <  2:318580/149‑1 (MQ=255)
                       tGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCata                                                                                                                           <  1:3957802/149‑1 (MQ=255)
                        gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCatat                                                                                                                          >  1:1443514/1‑149 (MQ=255)
                          agcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATcc                                                                                                                        <  2:1867397/149‑1 (MQ=255)
                          agcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATcc                                                                                                                        <  1:4243427/149‑1 (MQ=255)
                             gcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGt                                                                                                                     <  2:4750853/149‑1 (MQ=255)
                             gcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGt                                                                                                                     >  1:26939/1‑149 (MQ=255)
                             gcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGt                                                                                                                     <  1:8327/149‑1 (MQ=255)
                                gcgcGTCAGTACCGTGNGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                  >  2:2717525/1‑149 (MQ=255)
                                  gcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTc                                                                                                                <  1:857456/149‑1 (MQ=255)
                                   cgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCg                                                                                                               <  1:2214209/149‑1 (MQ=255)
                                    gTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGa                                                                                                              >  1:3887315/1‑149 (MQ=255)
                                     tCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGAt                                                                                                             >  1:774612/1‑149 (MQ=255)
                                     tCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGAt                                                                                                             >  1:3779797/1‑149 (MQ=255)
                                       aGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATaa                                                                                                           <  2:3935152/149‑1 (MQ=255)
                                         tACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACg                                                                                                         <  2:1906564/149‑1 (MQ=255)
                                          aCCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGt                                                                                                        <  2:3842236/149‑1 (MQ=255)
                                              tGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAcc                                                                                                    >  1:4600934/1‑149 (MQ=255)
                                                      aaTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAtt                                                                                            <  2:3527221/149‑1 (MQ=255)
                                                      aaTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAtt                                                                                            >  2:4345186/1‑149 (MQ=255)
                                                        tACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                          <  1:3567222/149‑1 (MQ=255)
                                                          ccGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGa                                                                                        <  2:3377232/149‑1 (MQ=255)
                                                          ccGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGa                                                                                        <  1:875414/149‑1 (MQ=255)
                                                           cGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGAc                                                                                       <  2:1074135/149‑1 (MQ=255)
                                                            gTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACa                                                                                      >  2:1090555/1‑149 (MQ=255)
                                                             ttCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAt                                                                                     >  2:1442786/1‑149 (MQ=255)
                                                                gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtctt                                                                                  >  1:3606210/1‑149 (MQ=255)
                                                                 ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttc                                                                                 >  2:1960775/1‑149 (MQ=255)
                                                                 ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttc                                                                                 >  1:1253682/1‑149 (MQ=255)
                                                                  gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                <  2:3319070/149‑1 (MQ=255)
                                                                  gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                <  1:551439/149‑1 (MQ=255)
                                                                  gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                <  1:2943278/149‑1 (MQ=255)
                                                                    aCTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTgg                                                                              >  2:1625114/1‑149 (MQ=255)
                                                                     cTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGc                                                                             <  1:2334457/149‑1 (MQ=255)
                                                                       gCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCt                                                                           >  1:1561232/1‑149 (MQ=255)
                                                                         ttCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTc                                                                         >  2:1294009/1‑149 (MQ=255)
                                                                         ttCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAACACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTc                                                                         >  2:660058/1‑149 (MQ=255)
                                                                          tctcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCg                                                                        >  1:1774725/1‑149 (MQ=255)
                                                                            tcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtc                                                                      <  2:2550541/149‑1 (MQ=255)
                                                                              ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcat                                                                    <  2:2238764/149‑1 (MQ=255)
                                                                              ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcat                                                                    >  2:3162011/1‑149 (MQ=255)
                                                                               gTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatc                                                                   >  2:4555271/1‑149 (MQ=255)
                                                                               gTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatc                                                                   >  1:3375620/1‑149 (MQ=255)
                                                                               gTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatc                                                                   >  2:1767788/1‑149 (MQ=255)
                                                                                tGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatca                                                                  <  2:1112368/149‑1 (MQ=255)
                                                                                 gcgcTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCAc                                                                 <  1:4696404/149‑1 (MQ=255)
                                                                                    cTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTCCGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGaa                                                              >  2:1706608/1‑149 (MQ=255)
                                                                                    cTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGaa                                                              >  1:3426069/1‑149 (MQ=255)
                                                                                      tACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATc                                                            >  2:3601490/1‑149 (MQ=255)
                                                                                      tACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATc                                                            <  2:4525921/149‑1 (MQ=255)
                                                                                         ggATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAgg                                                         >  1:3036584/1‑149 (MQ=255)
                                                                                           aTTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                       >  2:1760309/1‑149 (MQ=255)
                                                                                             tttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCgg                                                     <  1:3171579/149‑1 (MQ=255)
                                                                                               tGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTc                                                   <  1:1556783/149‑1 (MQ=255)
                                                                                               tGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTc                                                   <  1:4497263/149‑1 (MQ=255)
                                                                                                gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                  <  1:2739406/149‑1 (MQ=255)
                                                                                                  ccGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATg                                                <  2:1282739/149‑1 (MQ=255)
                                                                                                          tGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgt                                        >  2:2516531/1‑149 (MQ=255)
                                                                                                             ggCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGtgtg                                     >  2:500635/1‑149 (MQ=255)
                                                                                                              gCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTgg                                    >  2:1963583/1‑149 (MQ=255)
                                                                                                                tGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGaa                                  <  1:4141921/149‑1 (MQ=255)
                                                                                                                  catcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACg                                >  2:2390918/1‑149 (MQ=255)
                                                                                                                   atcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACgg                               >  2:1498863/1‑149 (MQ=255)
                                                                                                                    tcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGc                              >  1:1627905/1‑149 (MQ=255)
                                                                                                                     catATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCa                             <  1:1405018/149‑1 (MQ=255)
                                                                                                                      atatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAc                            <  1:2068330/149‑1 (MQ=255)
                                                                                                                       tatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAcc                           <  1:3764014/149‑1 (MQ=255)
                                                                                                                       tatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAcc                           >  2:4695580/1‑149 (MQ=255)
                                                                                                                         ttGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                         >  1:1041811/1‑149 (MQ=255)
                                                                                                                             cGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGt                     <  1:2547287/149‑1 (MQ=255)
                                                                                                                             cGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGt                     <  1:926658/149‑1 (MQ=255)
                                                                                                                                  cGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc                >  1:1489811/1‑149 (MQ=255)
                                                                                                                                  cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc                >  1:4051575/1‑149 (MQ=255)
                                                                                                                                  cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc                <  2:3230156/149‑1 (MQ=255)
                                                                                                                                      tttCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAttt            <  1:1962133/149‑1 (MQ=255)
                                                                                                                                       ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAtttt           <  1:3257700/149‑1 (MQ=255)
                                                                                                                                            gcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt      >  2:2628400/1‑149 (MQ=255)
                                                                                                                                            gcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt      >  2:154703/1‑149 (MQ=255)
                                                                                                                                             cagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCtt     <  2:3953931/149‑1 (MQ=255)
                                                                                                                                              agTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCttt    <  1:3493195/149‑1 (MQ=255)
                                                                                                                                               gTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtg   >  2:1490616/1‑149 (MQ=255)
                                                                                                                                                ttCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtga  >  2:4004857/1‑149 (MQ=255)
                                                                                                                                                ttCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCAAGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtga  >  2:3868670/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGA  >  CP009273/4471783‑4472075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑