Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,495,859 T→G 82.0% D252E (GAT→GAG)  yjhC → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,495,8590TG82.0% 690.3 / 99.1 239D252E (GAT→GAG) yjhCputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base G (82/114);  total (103/136)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 242 total aligned reads displayed.

GTCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAA  >  CP009273/4495711‑4496004
                                                                                                                                                    |                                                                                                                                                 
gTCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGat                                                                                                                                                   <  1:1664822/149‑1 (MQ=255)
     ggATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCa                                                                                                                                              >  1:3176005/1‑149 (MQ=255)
     ggATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCa                                                                                                                                              >  2:3059596/1‑149 (MQ=255)
      gATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCaa                                                                                                                                             <  2:1837242/149‑1 (MQ=255)
       aTTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAg                                                                                                                                            >  2:267177/1‑149 (MQ=255)
        ttttGCAATTAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCATGAAAACTAGCAACCTTAGAGTTGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGa                                                                                                                                           <  2:3367827/149‑1 (MQ=255)
         ttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGaa                                                                                                                                          <  2:2022031/149‑1 (MQ=255)
           ggCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAc                                                                                                                                        <  1:636899/149‑1 (MQ=255)
            gCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAca                                                                                                                                       <  2:1101170/149‑1 (MQ=255)
              aaTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagc                                                                                                                                     <  2:1147368/149‑1 (MQ=255)
               aTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagca                                                                                                                                    >  2:131211/1‑149 (MQ=255)
               aTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagca                                                                                                                                    <  1:3407581/149‑1 (MQ=255)
                tGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagcag                                                                                                                                   <  1:1417378/149‑1 (MQ=255)
                 gAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAgg                                                                                                                                  >  1:2126342/1‑149 (MQ=255)
                 gAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAgg                                                                                                                                  <  2:893062/149‑1 (MQ=255)
                  aaGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAggg                                                                                                                                 >  2:1136606/1‑149 (MQ=255)
                    gatgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTc                                                                                                                               >  1:403602/1‑149 (MQ=255)
                      tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCAc                                                                                                                             >  1:1856125/1‑149 (MQ=255)
                      tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCAc                                                                                                                             >  1:1760731/1‑149 (MQ=255)
                        atatGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACtt                                                                                                                           >  1:3601238/1‑149 (MQ=255)
                         tatGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTa                                                                                                                          <  2:4323756/149‑1 (MQ=255)
                          atGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAg                                                                                                                         >  1:2750335/1‑149 (MQ=255)
                               atttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTg                                                                                                                    <  1:1349742/149‑1 (MQ=255)
                                  tatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCg                                                                                                                 <  1:4493355/149‑1 (MQ=255)
                                   atGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCgg                                                                                                                <  1:798540/149‑1 (MQ=255)
                                   atGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTGAGGATTGGCgg                                                                                                                >  2:4434855/1‑149 (MQ=255)
                                    tGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGt                                                                                                               >  2:405777/1‑149 (MQ=255)
                                     gACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTc                                                                                                              >  1:4746926/1‑149 (MQ=255)
                                      aCCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCa                                                                                                             <  1:3869123/149‑1 (MQ=255)
                                         ttGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGAc                                                                                                          >  1:1538686/1‑149 (MQ=255)
                                         ttGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGAc                                                                                                          >  2:4220098/1‑149 (MQ=255)
                                           ggAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACaa                                                                                                        >  2:3359769/1‑149 (MQ=255)
                                               ttCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCa                                                                                                    <  1:2901269/149‑1 (MQ=255)
                                               ttCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCa                                                                                                    >  1:4095185/1‑149 (MQ=255)
                                               ttCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCa                                                                                                    <  2:2908009/149‑1 (MQ=255)
                                                tCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAt                                                                                                   <  2:526288/149‑1 (MQ=255)
                                                    gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                               <  1:2264416/149‑1 (MQ=255)
                                                      cAGGAAAACTAGCAACCTTAGTGTGGGGGAGTGCATTTAACGGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTg                                                                                             <  1:2570708/149‑1 (MQ=255)
                                                       aGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTgg                                                                                            <  2:1909020/149‑1 (MQ=255)
                                                        ggAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGt                                                                                           <  1:2816210/149‑1 (MQ=255)
                                                        ggAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGt                                                                                           <  2:856986/149‑1 (MQ=255)
                                                          aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTcc                                                                                         >  2:1257616/1‑149 (MQ=255)
                                                          aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTcc                                                                                         >  1:1020945/1‑149 (MQ=255)
                                                            aaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                       >  2:2392134/1‑149 (MQ=255)
                                                            aaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                       <  2:1913750/149‑1 (MQ=255)
                                                                aGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaaa                                                                                   <  1:4606356/149‑1 (MQ=255)
                                                                 gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGTGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAac                                                                                  >  2:3809912/1‑149 (MQ=255)
                                                                   aaCCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAacac                                                                                >  1:985197/1‑149 (MQ=255)
                                                                     ccTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaa                                                                              <  2:3225746/149‑1 (MQ=255)
                                                                       ttAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                            >  1:4467775/1‑149 (MQ=255)
                                                                          gagTGGGGGAGTGCATTTAACTGGCCGTAACATTATGTCATCATCAATGTAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaaga                                                                         <  2:3389076/149‑1 (MQ=255)
                                                                           agTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaagaa                                                                        <  2:4333415/149‑1 (MQ=255)
                                                                            gTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaagaag                                                                       <  1:1166379/149‑1 (MQ=255)
                                                                                 ggAGTGAATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgat                                                                  <  1:2511669/149‑1 (MQ=255)
                                                                                   aGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatga                                                                >  1:3452951/1‑149 (MQ=255)
                                                                                   aGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatga                                                                <  2:2209646/149‑1 (MQ=255)
                                                                                    gTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatgat                                                               <  2:115847/149‑1 (MQ=255)
                                                                                    gTGAATTTAACTGGCCGGAACATTATGTCATCATCAATTGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatgat                                                               <  2:2509000/149‑1 (MQ=255)
                                                                                       cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcgt                                                            <  2:1737693/149‑1 (MQ=255)
                                                                                       cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcgt                                                            <  1:1318382/149‑1 (MQ=255)
                                                                                       cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcgt                                                            <  1:4297098/149‑1 (MQ=255)
                                                                                       cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcgt                                                            <  1:3336418/149‑1 (MQ=255)
                                                                                         tttAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATTATGAtcgtcg                                                          >  2:3960967/1‑149 (MQ=255)
                                                                                          ttAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCgg                                                         <  2:3176018/149‑1 (MQ=255)
                                                                                            aaCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaa                                                       <  1:3059596/149‑1 (MQ=255)
                                                                                             aCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaaa                                                      >  1:737736/1‑149 (MQ=255)
                                                                                              cTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAg                                                     >  2:2737342/1‑149 (MQ=255)
                                                                                                ggCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGc                                                   >  2:4581234/1‑149 (MQ=255)
                                                                                                  ccGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCaa                                                 <  1:2322602/149‑1 (MQ=255)
                                                                                                    ggAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAata                                               >  1:4280214/1‑149 (MQ=255)
                                                                                                     gAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAatat                                              <  2:1800178/149‑1 (MQ=255)
                                                                                                     gAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAatat                                              >  1:568736/1‑149 (MQ=255)
                                                                                                       aCATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGa                                            <  1:3795590/149‑1 (MQ=255)
                                                                                                         aTTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGAcc                                          <  1:2361835/149‑1 (MQ=255)
                                                                                                           tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTc                                        >  2:1319781/1‑149 (MQ=255)
                                                                                                             tGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAg                                      >  1:535592/1‑149 (MQ=255)
                                                                                                             tGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAg                                      <  1:2113892/149‑1 (MQ=255)
                                                                                                               tcatcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaa                                    >  2:3633798/1‑149 (MQ=255)
                                                                                                                catcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaaa                                   <  1:298908/149‑1 (MQ=255)
                                                                                                                  tcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatg                                 <  2:3693250/149‑1 (MQ=255)
                                                                                                                  tcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatg                                 <  2:2008417/149‑1 (MQ=255)
                                                                                                                  tcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatg                                 <  2:2248928/149‑1 (MQ=255)
                                                                                                                  taatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatg                                 <  2:2138907/147‑1 (MQ=255)
                                                                                                                   catcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatgg                                >  1:3948278/1‑149 (MQ=255)
                                                                                                                     tcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              <  1:3757868/149‑1 (MQ=255)
                                                                                                                     tcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              <  1:189705/149‑1 (MQ=255)
                                                                                                                     tcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              <  1:1136606/149‑1 (MQ=255)
                                                                                                                     tcaATGGAACTAAACGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              <  2:1597711/149‑1 (MQ=255)
                                                                                                                      caATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatg                             >  2:7438/1‑149 (MQ=255)
                                                                                                                           gAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCt                        >  1:1005311/1‑149 (MQ=255)
                                                                                                                            aaCTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCta                       <  2:803309/149‑1 (MQ=255)
                                                                                                                               tAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                    >  1:1916098/1‑149 (MQ=255)
                                                                                                                                aaaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGc                   >  2:2701547/1‑149 (MQ=255)
                                                                                                                                aaaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGc                   >  2:4528999/1‑149 (MQ=255)
                                                                                                                                    gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCatat               <  1:131443/149‑1 (MQ=255)
                                                                                                                                    gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCatat               <  1:3213577/149‑1 (MQ=255)
                                                                                                                                       ctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGt            <  2:2966778/149‑1 (MQ=255)
                                                                                                                                            aaaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATcc       <  2:3297770/149‑1 (MQ=255)
                                                                                                                                                ttGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGa   >  1:1070922/1‑149 (MQ=255)
                                                                                                                                                 tGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGaa  <  2:1856125/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTCCAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAA  >  CP009273/4495711‑4496004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑