Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,511,163 C→A 6.3% M225I (ATG→ATT)  yjhF ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,511,1630CA 6.3% 1371.3 / 36.8 356M225I (ATG→ATT) yjhFputative transporter
Reads supporting (aligned to +/- strand):  ref base C (169/164);  new base A (11/12);  total (180/176)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GATCGTTGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATA  >  CP009273/4511016‑4511311
                                                                                                                                                   |                                                                                                                                                    
gATCGTTGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCa                                                                                                                                                     >  2:361448/1‑149 (MQ=255)
  tCGTTGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGAAtt                                                                                                                                                   >  2:4049413/1‑149 (MQ=255)
    gTTGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttc                                                                                                                                                 <  1:2326170/149‑1 (MQ=255)
      tGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttcgc                                                                                                                                               <  2:2948367/149‑1 (MQ=255)
      tGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttcgc                                                                                                                                               <  1:987208/149‑1 (MQ=255)
       gAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttcgct                                                                                                                                              >  2:898111/1‑149 (MQ=255)
        aaTTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttcgctt                                                                                                                                             >  1:1822424/1‑149 (MQ=255)
          ttACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCAtttcgctttc                                                                                                                                           <  2:3535983/149‑1 (MQ=255)
              cTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTg                                                                                                                                       >  2:1510042/1‑149 (MQ=255)
               tAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGa                                                                                                                                      >  1:2647070/1‑149 (MQ=255)
               tAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGa                                                                                                                                      >  1:2814095/1‑149 (MQ=255)
                aaGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGaa                                                                                                                                     >  1:3816677/1‑149 (MQ=255)
                  gAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATc                                                                                                                                   <  2:1570114/149‑1 (MQ=255)
                  gAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATc                                                                                                                                   <  1:2726042/149‑1 (MQ=255)
                    aCAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATcgg                                                                                                                                 >  2:4406496/1‑149 (MQ=255)
                     cAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATcggc                                                                                                                                <  2:1817934/149‑1 (MQ=255)
                     cAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATcggc                                                                                                                                <  2:1887227/149‑1 (MQ=255)
                       gTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATcggcgg                                                                                                                              >  1:2950388/1‑149 (MQ=255)
                       gTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATcggcgg                                                                                                                              >  1:2872820/1‑149 (MQ=255)
                        tATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCggg                                                                                                                             >  2:2178076/1‑149 (MQ=255)
                        tATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCggg                                                                                                                             <  1:1062803/149‑1 (MQ=255)
                          tCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTc                                                                                                                           <  2:1326690/149‑1 (MQ=255)
                           cACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTcg                                                                                                                          >  1:2419653/1‑149 (MQ=255)
                           cACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTcg                                                                                                                          >  1:2906864/1‑149 (MQ=255)
                               ttGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGcc                                                                                                                      >  2:2412006/1‑149 (MQ=255)
                                tGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCg                                                                                                                     >  1:1568142/1‑149 (MQ=255)
                                tGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCg                                                                                                                     >  1:3957855/1‑149 (MQ=255)
                                  tAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCgag                                                                                                                   >  1:143618/1‑149 (MQ=255)
                                     aaTAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCgagaga                                                                                                                >  2:3790519/1‑149 (MQ=255)
                                      aTAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGagagag                                                                                                               <  1:4574662/149‑1 (MQ=255)
                                       tAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAgg                                                                                                              >  1:502696/1‑149 (MQ=255)
                                        aaGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGc                                                                                                             >  1:759613/1‑149 (MQ=255)
                                         aGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCt                                                                                                            >  2:262637/1‑149 (MQ=255)
                                         aGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCt                                                                                                            >  2:2655336/1‑149 (MQ=255)
                                         aGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCt                                                                                                            >  1:4773796/1‑149 (MQ=255)
                                          gCCTGAATCCGCGACACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCta                                                                                                           >  2:4680158/1‑149 (MQ=255)
                                            cTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCtata                                                                                                         <  2:3144611/149‑1 (MQ=255)
                                             tGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTatat                                                                                                        <  2:2574849/149‑1 (MQ=255)
                                             tGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTatat                                                                                                        <  1:3565238/149‑1 (MQ=255)
                                              gAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATAtt                                                                                                       <  1:406034/149‑1 (MQ=255)
                                                aTCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTcc                                                                                                     >  1:1864853/1‑149 (MQ=255)
                                                 tCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTccc                                                                                                    >  1:2115598/1‑149 (MQ=255)
                                                  ccGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTcccc                                                                                                   <  2:274622/149‑1 (MQ=255)
                                                    gcgGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCctc                                                                                                 <  1:2982677/149‑1 (MQ=255)
                                                     cgGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCctct                                                                                                <  2:4283548/149‑1 (MQ=255)
                                                     cgGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCctct                                                                                                <  2:1460386/149‑1 (MQ=255)
                                                     cgGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCctct                                                                                                <  2:1004359/149‑1 (MQ=255)
                                                       gCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttt                                                                                              >  2:3415468/1‑149 (MQ=255)
                                                         aCTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttttt                                                                                            <  1:1831791/149‑1 (MQ=255)
                                                         aCTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttttt                                                                                            >  2:2531145/1‑149 (MQ=255)
                                                          cTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCtttttt                                                                                           >  1:1665667/1‑149 (MQ=255)
                                                          cTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCtttttt                                                                                           <  2:4473103/149‑1 (MQ=255)
                                                                gCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGc                                                                                     >  2:3344797/1‑149 (MQ=255)
                                                                  aTGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGt                                                                                   <  2:2990854/149‑1 (MQ=255)
                                                                  aTGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGt                                                                                   >  1:3463146/1‑149 (MQ=255)
                                                                        cATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGaa                                                                             <  1:3349038/149‑1 (MQ=255)
                                                                          tCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGaata                                                                           >  2:4243324/1‑149 (MQ=255)
                                                                           cTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGaataa                                                                          <  2:1756326/149‑1 (MQ=255)
                                                                             ggATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATg                                                                        >  2:2821299/1‑149 (MQ=255)
                                                                             ggATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATg                                                                        <  1:3202570/149‑1 (MQ=255)
                                                                                  atgGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTg                                                                   <  2:4354527/149‑1 (MQ=255)
                                                                                  atgGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTg                                                                   <  2:4765427/149‑1 (MQ=255)
                                                                                       gACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGa                                                              >  2:3235475/1‑149 (MQ=255)
                                                                                        aCAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGaa                                                             >  2:2284033/1‑149 (MQ=255)
                                                                                               ccATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTc                                                      >  2:3946966/1‑149 (MQ=255)
                                                                                               ccATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTc                                                      >  1:4665034/1‑149 (MQ=255)
                                                                                                 aTCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCaa                                                    >  1:1089399/1‑149 (MQ=255)
                                                                                                  tCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAAt                                                   >  2:1844326/1‑149 (MQ=255)
                                                                                                  tCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAAt                                                   <  2:1477436/149‑1 (MQ=255)
                                                                                                    aGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATAc                                                 <  2:136483/149‑1 (MQ=255)
                                                                                                     ggATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGACTAATGCGCTGGCAGAATTTATTCAATAcc                                                >  2:2425706/1‑149 (MQ=255)
                                                                                                      gATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCg                                               >  1:3629014/1‑149 (MQ=255)
                                                                                                          aCCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGcc                                           <  2:3680980/149‑1 (MQ=255)
                                                                                                          aCCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGcc                                           <  2:1290704/149‑1 (MQ=255)
                                                                                                             ggCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCa                                        >  1:625457/1‑149 (MQ=255)
                                                                                                               cagcaTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCaaa                                      <  2:862256/149‑1 (MQ=255)
                                                                                                               cagcaTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCaaa                                      <  1:457566/149‑1 (MQ=255)
                                                                                                               cagcaTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCaaa                                      <  1:1301395/149‑1 (MQ=255)
                                                                                                                  caTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGaa                                   <  1:1215120/149‑1 (MQ=255)
                                                                                                                     tGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATaa                                <  1:4266431/149‑1 (MQ=255)
                                                                                                                      gCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATaaa                               >  1:690393/1‑149 (MQ=255)
                                                                                                                      gCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATaaa                               <  1:1112183/149‑1 (MQ=255)
                                                                                                                      gCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATaaa                               >  1:3090455/1‑149 (MQ=255)
                                                                                                                         gTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGt                            >  1:3061551/1‑149 (MQ=255)
                                                                                                                            agaAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTgg                         <  1:263151/149‑1 (MQ=255)
                                                                                                                             gaAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGa                        >  2:2025249/1‑149 (MQ=255)
                                                                                                                                      tCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACa               <  2:2109659/149‑1 (MQ=255)
                                                                                                                                        ccAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAAt             >  2:1128445/1‑149 (MQ=255)
                                                                                                                                        ccAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAAt             <  1:2411885/149‑1 (MQ=255)
                                                                                                                                        ccAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAAt             <  2:779170/149‑1 (MQ=255)
                                                                                                                                             gCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAc        >  1:540221/1‑149 (MQ=255)
                                                                                                                                             gCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAc        <  1:4477304/149‑1 (MQ=255)
                                                                                                                                             gCCTGGAATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAc        <  1:2079608/149‑1 (MQ=255)
                                                                                                                                              ccTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAcc       <  1:1023902/149‑1 (MQ=255)
                                                                                                                                              ccTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAcc       >  2:3452824/1‑149 (MQ=255)
                                                                                                                                              ccTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAcc       <  2:2667218/149‑1 (MQ=255)
                                                                                                                                               cTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCg      >  2:4202677/1‑149 (MQ=255)
                                                                                                                                                tGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGt     >  2:1667067/1‑149 (MQ=255)
                                                                                                                                                 ggCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGta    >  1:1944894/1‑149 (MQ=255)
                                                                                                                                                 ggCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGta    >  1:2490605/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATCGTTGAATTACCTAAGAACAGTATCACGTTGTAAAATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATA  >  CP009273/4511016‑4511311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑