Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 372,581 G→T 86.6% A118A (GCG→GCT)  yaiL → DUF2058 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273372,5810GT86.6% 751.0 / 120.8 306A118A (GCG→GCT) yaiLDUF2058 family protein
Reads supporting (aligned to +/- strand):  ref base G (23/18);  new base T (106/159);  total (129/177)
Fisher's exact test for biased strand distribution p-value = 6.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 306 total aligned reads displayed.

TAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACC  >  CP009273/372434‑372726
                                                                                                                                                   |                                                                                                                                                 
 aaaGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTAt                                                                                                                                                 >  1:574505/1‑149 (MQ=255)
 aaaGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTAt                                                                                                                                                 <  1:691262/149‑1 (MQ=255)
  aaGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTAtt                                                                                                                                                >  1:1234695/1‑149 (MQ=255)
    gCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGc                                                                                                                                              <  1:190698/149‑1 (MQ=255)
        aGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGc                                                                                                                                          >  2:2215663/1‑149 (MQ=255)
         ggTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCt                                                                                                                                         <  1:2882433/149‑1 (MQ=255)
            gAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCttgt                                                                                                                                      <  2:3433826/149‑1 (MQ=255)
             aaGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCttgtt                                                                                                                                     <  2:2091647/149‑1 (MQ=255)
             aaGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCttgtt                                                                                                                                     <  1:3573246/149‑1 (MQ=255)
             aaGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCttgtt                                                                                                                                     >  2:2028571/1‑149 (MQ=255)
                cagcTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGt                                                                                                                                  <  2:831989/149‑1 (MQ=255)
                  gcTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCg                                                                                                                                >  1:4571344/1‑149 (MQ=255)
                   cTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGa                                                                                                                               >  2:1914936/1‑149 (MQ=255)
                   cTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGa                                                                                                                               >  1:2861920/1‑149 (MQ=255)
                     tATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATa                                                                                                                             <  1:478664/149‑1 (MQ=255)
                     tATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATa                                                                                                                             >  2:3336250/1‑149 (MQ=255)
                      aTTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATaa                                                                                                                            <  1:3720299/149‑1 (MQ=255)
                      aTTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATaa                                                                                                                            >  1:1575425/1‑149 (MQ=255)
                        tGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACa                                                                                                                          >  2:4733919/1‑149 (MQ=255)
                         gAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACaa                                                                                                                         <  2:1056739/149‑1 (MQ=255)
                          aaaTGAACCGAATCACCATTGCCACTGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAAt                                                                                                                        <  1:519059/149‑1 (MQ=255)
                          aaaTGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAAt                                                                                                                        >  2:4471351/1‑149 (MQ=255)
                          aaaTGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAAt                                                                                                                        >  2:2172563/1‑149 (MQ=255)
                           aaTGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATa                                                                                                                       <  1:1427988/149‑1 (MQ=255)
                              gAACCGAATCACCAGTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGCTAACAATAGCg                                                                                                                    <  2:4517032/149‑1 (MQ=255)
                                aCCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGaa                                                                                                                  >  2:415863/1‑149 (MQ=255)
                                 ccGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAg                                                                                                                 <  1:4418520/149‑1 (MQ=255)
                                  cGAGTCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTATTGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAgg                                                                                                                <  1:2916661/149‑1 (MQ=255)
                                  cGAATCACCATTGCCAATGGCGTTATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAgg                                                                                                                <  2:1604408/149‑1 (MQ=255)
                                   gAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGt                                                                                                               <  2:305746/149‑1 (MQ=255)
                                       cACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAAt                                                                                                           >  2:235338/1‑149 (MQ=255)
                                        aCCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGTTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATa                                                                                                          <  1:922231/149‑1 (MQ=255)
                                        aCCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATa                                                                                                          <  2:1699403/149‑1 (MQ=255)
                                         ccATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATAc                                                                                                         <  1:4447711/149‑1 (MQ=255)
                                           aTTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGc                                                                                                       <  1:1150775/149‑1 (MQ=255)
                                           aTTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGc                                                                                                       <  2:4551413/149‑1 (MQ=255)
                                            ttGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGcc                                                                                                      >  1:2584408/1‑149 (MQ=255)
                                                cAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCatta                                                                                                  <  2:4578385/149‑1 (MQ=255)
                                                  aTGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCattatt                                                                                                <  1:4670584/149‑1 (MQ=255)
                                                       gATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGc                                                                                           >  2:3528210/1‑149 (MQ=255)
                                                        atatTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGcc                                                                                          >  1:2167605/1‑149 (MQ=255)
                                                         tatTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGGTGATTAATGGTCGTCTGGCTATTGCCCGCGTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCa                                                                                         >  2:2581313/1‑149 (MQ=255)
                                                             ggTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGt                                                                                     <  1:2396467/149‑1 (MQ=255)
                                                                tttAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGc                                                                                  <  2:4294566/149‑1 (MQ=255)
                                                                   aaCTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGa                                                                               <  1:2215663/149‑1 (MQ=255)
                                                                    aCTTCACTGACGGCAATCTGATTAAGAAGATTTATGTCGATAAGCTCACACAGGCTCAGTTGATTAATGGTCGTCTGGATATTGCCCGCTTGATGGTCGATAACAATAGCTAAGGTGAATACGACATTATTACCGCCAGCGTTGCCGAt                                                                              >  2:2368474/1‑149 (MQ=255)
                                                                     cTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATa                                                                             <  1:559715/149‑1 (MQ=255)
                                                                         aCTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAAt                                                                         >  2:21111/1‑149 (MQ=255)
                                                                          cTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTGGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAAtt                                                                        <  1:1564893/149‑1 (MQ=255)
                                                                          cTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAAtt                                                                        >  2:1333706/1‑149 (MQ=255)
                                                                           tGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTg                                                                       >  1:3102456/1‑149 (MQ=255)
                                                                            gACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGATGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGc                                                                      <  1:1676691/149‑1 (MQ=255)
                                                                             aCGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCt                                                                     <  2:136506/149‑1 (MQ=255)
                                                                             aCGGCAATCTGAGTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCt                                                                     <  2:4094621/149‑1 (MQ=255)
                                                                                gCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAg                                                                  >  2:490410/1‑149 (MQ=255)
                                                                                   aTCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAgcgc                                                               >  2:3125427/1‑149 (MQ=255)
                                                                                       gATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATg                                                           <  1:2074665/149‑1 (MQ=255)
                                                                                       gATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATg                                                           >  1:4508639/1‑149 (MQ=255)
                                                                                       gATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATg                                                           >  1:3450783/1‑149 (MQ=255)
                                                                                         ttAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGcc                                                         >  2:323494/1‑149 (MQ=255)
                                                                                           aagaagATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATTGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCcag                                                       <  1:3364319/149‑1 (MQ=255)
                                                                                            agaagaTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCcagc                                                      >  2:1565920/1‑149 (MQ=255)
                                                                                               agaTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGt                                                   >  1:2082227/1‑149 (MQ=255)
                                                                                               agaTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGt                                                   >  1:3053047/1‑149 (MQ=255)
                                                                                               agaTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGt                                                   >  1:486574/1‑149 (MQ=255)
                                                                                                gaTTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGta                                                  >  1:3241070/1‑149 (MQ=255)
                                                                                                   ttttGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAg                                               <  1:592067/149‑1 (MQ=255)
                                                                                                      tGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAgtgt                                            <  1:16522/149‑1 (MQ=255)
                                                                                                      tGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAgtgt                                            <  2:914561/149‑1 (MQ=255)
                                                                                                        tCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTg                                          >  1:1049059/1‑149 (MQ=255)
                                                                                                        tCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTg                                          >  1:2347499/1‑149 (MQ=255)
                                                                                                        tCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTg                                          >  1:761276/1‑149 (MQ=255)
                                                                                                          gATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGca                                        >  2:582209/1‑149 (MQ=255)
                                                                                                           aTAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGcac                                       >  2:2945527/1‑149 (MQ=255)
                                                                                                           aTAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGcac                                       >  1:1756996/1‑149 (MQ=255)
                                                                                                           aTAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGcac                                       <  1:815880/149‑1 (MQ=255)
                                                                                                              aGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAgc                                    >  2:1786752/1‑149 (MQ=255)
                                                                                                                     acaGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCa                             <  2:1085537/149‑1 (MQ=255)
                                                                                                                     acaGGCGCAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCa                             <  2:4672047/149‑1 (MQ=255)
                                                                                                                        ggCGCAGGTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAgcg                          >  1:1814445/1‑149 (MQ=255)
                                                                                                                         gcgcAGTTGATTAATGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAgcgc                         >  2:737269/1‑149 (MQ=255)
                                                                                                                             aGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGaa                     <  2:142336/149‑1 (MQ=255)
                                                                                                                                     aaTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGa             <  1:3906936/149‑1 (MQ=255)
                                                                                                                                     aaTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGa             >  2:2632071/1‑149 (MQ=255)
                                                                                                                                     aaTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGa             <  1:4269234/149‑1 (MQ=255)
                                                                                                                                      aTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGAt            >  2:3977267/1‑149 (MQ=255)
                                                                                                                                      aTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGAt            <  2:513471/149‑1 (MQ=255)
                                                                                                                                      aTGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGAt            <  2:574500/149‑1 (MQ=255)
                                                                                                                                      aTGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGAt            >  1:2719887/1‑149 (MQ=255)
                                                                                                                                       tGGTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATg           >  1:2516957/1‑149 (MQ=255)
                                                                                                                                        ggTCGTCTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGa          <  2:4333559/149‑1 (MQ=255)
                                                                                                                                         gtcgtcTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGaa         <  1:1979840/149‑1 (MQ=255)
                                                                                                                                          tcgtcTGGCTATTGCCCGCTTGTTGGTCGATAACAATCGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAg        <  2:4107409/149‑1 (MQ=255)
                                                                                                                                          tcgtcTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAg        >  2:1144715/1‑149 (MQ=255)
                                                                                                                                           cgtcTGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAga       <  2:1234695/149‑1 (MQ=255)
                                                                                                                                            gtcTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAgac      >  2:4695783/1‑149 (MQ=255)
                                                                                                                                            gtcTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAgac      >  2:3817678/1‑149 (MQ=255)
                                                                                                                                               tGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAgacgac   <  1:98350/149‑1 (MQ=255)
                                                                                                                                               tGGCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAgacgac   <  1:1476716/149‑1 (MQ=255)
                                                                                                                                                ggCTATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGAcc  <  1:2643352/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAAAGCTCAGGTGAAGCAGCTTATTGAAATGAACCGAATCACCATTGCCAATGGCGATATTGGTTTTAACTTCACTGACGGCAATCTGATTAAGAAGATTTTTGTCGATAAGCTCACACAGGCGCAGTTGATTAATGGTCGTCTGGCGATTGCCCGCTTGTTGGTCGATAACAATAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACC  >  CP009273/372434‑372726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑