Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,255 A→C 83.0% L95L (CTT→CTG)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,2550AC83.0% 911.6 / 119.3 311L95L (CTT→CTG) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base A (20/33);  new base C (127/131);  total (147/164)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 313 total aligned reads displayed.

TTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATA  >  CP009273/4584108‑4584403
                                                                                                                                                   |                                                                                                                                                    
ttCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGaa                                                                                                                                                     <  2:3768308/149‑1 (MQ=255)
 tCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAg                                                                                                                                                    <  1:4338304/149‑1 (MQ=255)
     cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                                <  1:1588920/149‑1 (MQ=255)
     cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                                >  2:590273/1‑149 (MQ=255)
     cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                                >  2:4722272/1‑149 (MQ=255)
     cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTgc                                                                                                                                                <  1:4120674/149‑1 (MQ=255)
     cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTgc                                                                                                                                                <  1:3575751/149‑1 (MQ=255)
     cAATCCCCAGACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                                <  1:2738882/149‑1 (MQ=255)
                ccGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTa                                                                                                                                     <  2:3723519/149‑1 (MQ=255)
                  gATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAg                                                                                                                                   <  1:1034158/149‑1 (MQ=255)
                    tACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                                 <  2:4084394/149‑1 (MQ=255)
                     aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGa                                                                                                                                <  2:2366568/149‑1 (MQ=255)
                        cACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGACCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTACGCGAGTg                                                                                                                             >  2:3813895/1‑149 (MQ=255)
                         aCCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                            >  1:3772914/1‑149 (MQ=255)
                         aCCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                            >  1:3141726/1‑149 (MQ=255)
                         aCCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                            <  2:4250266/149‑1 (MQ=255)
                           cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaa                                                                                                                          <  2:3482017/149‑1 (MQ=255)
                           cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACAGTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaa                                                                                                                          <  2:4466152/149‑1 (MQ=255)
                            aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                         <  2:1284725/149‑1 (MQ=255)
                            aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGACTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGTCGTGGGAATTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                         >  2:241843/1‑149 (MQ=255)
                                cGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACg                                                                                                                     <  1:2264535/149‑1 (MQ=255)
                                   aCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGgag                                                                                                                  >  2:3215586/1‑149 (MQ=255)
                                      gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGc                                                                                                               >  1:4230986/1‑149 (MQ=255)
                                       cGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCa                                                                                                              <  1:932960/149‑1 (MQ=255)
                                        gTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                             >  2:1437986/1‑149 (MQ=255)
                                         tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaaa                                                                                                            <  1:338954/149‑1 (MQ=255)
                                           aaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgc                                                                                                          >  1:2536562/1‑149 (MQ=255)
                                           aaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgc                                                                                                          <  2:4158966/149‑1 (MQ=255)
                                           aaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgc                                                                                                          <  2:4313857/149‑1 (MQ=255)
                                           aaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgc                                                                                                          >  1:2812481/1‑149 (MQ=255)
                                             gTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcgc                                                                                                        <  2:756149/149‑1 (MQ=255)
                                              ttGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGcc                                                                                                       <  1:3663453/149‑1 (MQ=255)
                                              ttGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGcc                                                                                                       <  1:1216784/149‑1 (MQ=255)
                                                 tgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCCGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAt                                                                                                    <  2:2808292/149‑1 (MQ=255)
                                                 tgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAt                                                                                                    <  2:3194700/149‑1 (MQ=255)
                                                      cATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCgg                                                                                               <  2:1521415/149‑1 (MQ=255)
                                                      cATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCgg                                                                                               >  2:1980309/1‑149 (MQ=255)
                                                       aTGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGt                                                                                              <  1:1944053/149‑1 (MQ=255)
                                                        tGCCAGACAGTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTg                                                                                             <  2:2186100/149‑1 (MQ=255)
                                                            aGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                         >  2:2726377/1‑149 (MQ=255)
                                                            aGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                         >  2:2596243/1‑149 (MQ=255)
                                                             gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACt                                                                                        >  2:3027436/1‑149 (MQ=255)
                                                             gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACt                                                                                        >  2:4109964/1‑149 (MQ=255)
                                                                 ttGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAAt                                                                                    >  2:3505223/1‑149 (MQ=255)
                                                                 ttGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAAt                                                                                    <  1:1523979/149‑1 (MQ=255)
                                                                  tGCCTGGAACCGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATCGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATc                                                                                   <  1:4446851/149‑1 (MQ=255)
                                                                   gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                                  >  2:3797495/1‑149 (MQ=255)
                                                                   gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                                  <  2:2943166/149‑1 (MQ=255)
                                                                     cTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                                >  1:2961315/1‑149 (MQ=255)
                                                                     cTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                                >  1:878897/1‑149 (MQ=255)
                                                                     cTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                                >  1:3305702/1‑149 (MQ=255)
                                                                     cTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                                >  1:116933/1‑149 (MQ=255)
                                                                       ggAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAt                                                                              >  1:3416464/1‑149 (MQ=255)
                                                                       ggAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAt                                                                              <  2:1705515/149‑1 (MQ=255)
                                                                           cAGTGACCAGAAGAACATCCCCAGTCCCAGCATCTGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttctt                                                                          <  1:4037705/149‑1 (MQ=255)
                                                                              tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCAc                                                                       >  2:1858607/1‑149 (MQ=255)
                                                                                      agaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGtt                                                               >  2:3633004/1‑149 (MQ=255)
                                                                                      agaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGtt                                                               >  2:4751512/1‑149 (MQ=255)
                                                                                       gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                              >  2:1358891/1‑149 (MQ=255)
                                                                                        aaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaa                                                             >  2:2567790/1‑149 (MQ=255)
                                                                                        aaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaa                                                             <  2:2674509/149‑1 (MQ=255)
                                                                                           aTCCCCAGTCCCAGCCTCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAtt                                                          <  2:3998618/149‑1 (MQ=255)
                                                                                             ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCTCGAATCGTTAAATTTg                                                        >  1:3659998/1‑149 (MQ=255)
                                                                                               ccACTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                      <  2:4662413/149‑1 (MQ=255)
                                                                                                ccgTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCACTTCTTCACGAATCGTTAAATTTGCTa                                                     <  2:1597100/147‑1 (MQ=255)
                                                                                                cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTa                                                     <  2:3269479/149‑1 (MQ=255)
                                                                                                cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTa                                                     >  1:3322228/1‑149 (MQ=255)
                                                                                                 aGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAc                                                    <  1:1838076/149‑1 (MQ=255)
                                                                                                    cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                                 >  2:1318231/1‑149 (MQ=255)
                                                                                                    cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                                 <  1:786562/149‑1 (MQ=255)
                                                                                                    cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                                 <  1:1492958/149‑1 (MQ=255)
                                                                                                     ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                                >  1:999783/1‑149 (MQ=255)
                                                                                                     ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                                >  1:2353579/1‑149 (MQ=255)
                                                                                                        gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                             <  2:4390121/149‑1 (MQ=255)
                                                                                                        gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                             <  1:563753/149‑1 (MQ=255)
                                                                                                          aTCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGa                                           >  2:3697546/1‑149 (MQ=255)
                                                                                                            cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAGCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAAc                                         <  1:2047582/149‑1 (MQ=255)
                                                                                                             aGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACt                                        >  1:2995866/1‑149 (MQ=255)
                                                                                                                 gTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCgg                                    <  1:1763701/149‑1 (MQ=255)
                                                                                                                  tGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                                   >  1:1066879/1‑149 (MQ=255)
                                                                                                                  tGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                                   >  1:3963169/1‑149 (MQ=255)
                                                                                                                   gggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTc                                  <  1:4135471/149‑1 (MQ=255)
                                                                                                                       cTTTTCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCAGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                              <  2:1244366/149‑1 (MQ=255)
                                                                                                                        tttACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGAt                             <  2:490285/149‑1 (MQ=255)
                                                                                                                         ttACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATa                            <  2:2323560/149‑1 (MQ=255)
                                                                                                                          tACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATaa                           <  1:1453090/149‑1 (MQ=255)
                                                                                                                           aCGATCCTATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                          <  1:2929606/149‑1 (MQ=255)
                                                                                                                           aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                          <  2:2552368/149‑1 (MQ=255)
                                                                                                                           aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                          <  1:1869472/149‑1 (MQ=255)
                                                                                                                               tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                      >  2:911209/1‑149 (MQ=255)
                                                                                                                               tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                      <  1:2083349/149‑1 (MQ=255)
                                                                                                                                ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                     >  2:1778573/1‑149 (MQ=255)
                                                                                                                                ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                     <  2:2782536/149‑1 (MQ=255)
                                                                                                                                 cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta                    >  2:1827124/1‑149 (MQ=255)
                                                                                                                                   aTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc                  <  1:4674628/149‑1 (MQ=255)
                                                                                                                                   aTAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc                  >  2:1889220/1‑149 (MQ=255)
                                                                                                                                      gTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgcc               >  1:669223/1‑149 (MQ=255)
                                                                                                                                               aaGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaat      <  1:4735488/149‑1 (MQ=255)
                                                                                                                                                  gAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataat   <  2:1188391/149‑1 (MQ=255)
                                                                                                                                                   aaGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAataata  <  1:356466/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATA  >  CP009273/4584108‑4584403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑