Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 400,594 G→T 86.8% V169V (GTC→GTA)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,5940GT86.8% 715.8 / 121.2 305V169V (GTC→GTA) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (24/16);  new base T (110/155);  total (134/171)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 306 total aligned reads displayed.

GTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCG  >  CP009273/400448‑400742
                                                                                                                                                  |                                                                                                                                                    
gTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                    <  2:2070144/149‑1 (MQ=255)
gTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                    <  2:4164587/149‑1 (MQ=255)
             tttttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatc                                                                                                                                       >  1:3637601/1‑149 (MQ=255)
               tttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatcat                                                                                                                                     <  1:4727598/149‑1 (MQ=255)
                ttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatcatc                                                                                                                                    <  2:2597848/149‑1 (MQ=255)
                ttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatcatc                                                                                                                                    <  1:2306477/149‑1 (MQ=255)
                     gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTc                                                                                                                               >  1:4176259/1‑149 (MQ=255)
                     gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTc                                                                                                                               >  2:823648/1‑149 (MQ=255)
                      aaCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCa                                                                                                                              >  2:3870224/1‑149 (MQ=255)
                      aaCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCa                                                                                                                              >  2:4723385/1‑149 (MQ=255)
                      aaCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGAAAAACAAAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCa                                                                                                                              >  1:4086951/1‑149 (MQ=255)
                       aCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAg                                                                                                                             >  2:3730591/1‑149 (MQ=255)
                        cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGc                                                                                                                            <  1:657371/149‑1 (MQ=255)
                        cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGc                                                                                                                            >  1:2032682/1‑149 (MQ=255)
                         ccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCa                                                                                                                           >  2:3069525/1‑149 (MQ=255)
                          cATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCaa                                                                                                                          <  1:3230310/149‑1 (MQ=255)
                           aTTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAt                                                                                                                         >  2:1585214/1‑149 (MQ=255)
                            ttACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                        >  2:2935355/1‑149 (MQ=255)
                             tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                       <  2:191389/149‑1 (MQ=255)
                              aCCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                      >  2:1716952/1‑149 (MQ=255)
                                cGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACtt                                                                                                                    <  1:1916053/149‑1 (MQ=255)
                                  ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                                  <  1:3976088/149‑1 (MQ=255)
                                  ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                                  >  2:3676499/1‑149 (MQ=255)
                                    tGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGc                                                                                                                <  1:2176724/149‑1 (MQ=255)
                                    tGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGc                                                                                                                >  1:3655048/1‑149 (MQ=255)
                                    tGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGc                                                                                                                >  1:3275123/1‑149 (MQ=255)
                                     gAGCGGCAAATTTAGACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCt                                                                                                               <  2:1252643/149‑1 (MQ=255)
                                       gCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTc                                                                                                             <  2:4517609/149‑1 (MQ=255)
                                            aaaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaa                                                                                                        >  2:4786313/1‑149 (MQ=255)
                                             aaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                       >  1:268478/1‑149 (MQ=255)
                                              aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAag                                                                                                      <  2:1165037/149‑1 (MQ=255)
                                              aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAag                                                                                                      <  2:3788477/149‑1 (MQ=255)
                                                 tataCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcag                                                                                                   >  1:3932556/1‑149 (MQ=255)
                                                   taCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                                 >  2:2666643/1‑149 (MQ=255)
                                                        cTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                            <  1:1586905/149‑1 (MQ=255)
                                                         tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                           <  1:2785512/149‑1 (MQ=255)
                                                         tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                           >  2:4290129/1‑149 (MQ=255)
                                                         tGGGCGCGTGGCAGCCCGCCCCGCCCCGCGGCGACGGCCATCGCTACGAGAAACATAAATACGGAGGCTGGCGAAGAACCGCTCACACCTACCTCCGGGTGGATCATCGGCTCAGCATTTACTTCCGCTGCGCCAAAGCAGCGGaaaaa                                                                                           <  2:4241789/149‑2 (MQ=255)
                                                          gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAata                                                                                          >  1:2485198/1‑149 (MQ=255)
                                                           ggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAatat                                                                                         <  1:2636087/149‑1 (MQ=255)
                                                            gcgcGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATAtt                                                                                        <  1:4363888/149‑1 (MQ=255)
                                                             cgcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                       <  2:2401303/149‑1 (MQ=255)
                                                             cgcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                       <  1:1607748/149‑1 (MQ=255)
                                                             cgcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                       <  2:666533/149‑1 (MQ=255)
                                                                  ggCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGcac                                                                                  <  2:3549304/149‑1 (MQ=255)
                                                                  ggCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGcac                                                                                  <  1:4087467/149‑1 (MQ=255)
                                                                     aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGACAATATTCAGCACATc                                                                               >  2:605026/1‑149 (MQ=255)
                                                                     aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATc                                                                               >  1:1030419/1‑149 (MQ=255)
                                                                       cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAg                                                                             <  2:4625830/149‑1 (MQ=255)
                                                                        ccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAATCAGCGGAAAATATTCAGCACATCAGc                                                                            >  1:1452337/1‑149 (MQ=255)
                                                                        ccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGc                                                                            <  2:278555/149‑1 (MQ=255)
                                                                          gCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCgg                                                                          <  2:2394379/149‑1 (MQ=255)
                                                                          gCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCgg                                                                          <  1:2419257/149‑1 (MQ=255)
                                                                             cAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAt                                                                       <  2:703754/149‑1 (MQ=255)
                                                                              aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtc                                                                      >  1:3744504/1‑149 (MQ=255)
                                                                              aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtc                                                                      <  2:3844766/149‑1 (MQ=255)
                                                                                    gcggcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTgg                                                                >  2:3391789/1‑149 (MQ=255)
                                                                                       gcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGt                                                             <  1:594557/149‑1 (MQ=255)
                                                                                        cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGtt                                                            <  1:1203980/149‑1 (MQ=255)
                                                                                        cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGtt                                                            <  1:1973748/149‑1 (MQ=255)
                                                                                        cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGtt                                                            <  1:751942/149‑1 (MQ=255)
                                                                                        cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGtt                                                            <  1:4038471/149‑1 (MQ=255)
                                                                                         gTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTa                                                           <  1:709184/149‑1 (MQ=255)
                                                                                              ccATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgc                                                      >  1:4565439/1‑149 (MQ=255)
                                                                                                  cGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGt                                                  <  1:976344/149‑1 (MQ=255)
                                                                                                   gCTTCGATAAACAGAAATACGTAGGCTGGCGAAGAACCGCTCACACCTCCCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                                 <  1:3472468/149‑1 (MQ=255)
                                                                                                    cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGttt                                                <  1:1739728/149‑1 (MQ=255)
                                                                                                     ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                               <  1:4407926/149‑1 (MQ=255)
                                                                                                     ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                               >  2:2863785/1‑149 (MQ=255)
                                                                                                     ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                               <  1:2557253/149‑1 (MQ=255)
                                                                                                     ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                               >  1:3828927/1‑149 (MQ=255)
                                                                                                        gATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCg                                            <  2:1477928/149‑1 (MQ=255)
                                                                                                           aaaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                         >  1:3685785/1‑149 (MQ=255)
                                                                                                           aaaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                         >  1:500584/1‑149 (MQ=255)
                                                                                                           aaaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                         <  1:3326067/149‑1 (MQ=255)
                                                                                                            aaCATAACTACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTAc                                        <  2:1990326/149‑1 (MQ=255)
                                                                                                               aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGa                                     >  1:4266031/1‑149 (MQ=255)
                                                                                                               aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGa                                     >  1:3167859/1‑149 (MQ=255)
                                                                                                                tAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                    <  2:1658358/149‑1 (MQ=255)
                                                                                                                tAAAGACGGAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                    <  2:930991/149‑1 (MQ=255)
                                                                                                                 aaaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAgg                                   >  2:1683531/1‑149 (MQ=255)
                                                                                                                  aaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGt                                  >  2:2424435/1‑149 (MQ=255)
                                                                                                                   aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCGCATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTc                                 <  2:2218699/149‑1 (MQ=255)
                                                                                                                      cGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATc                              <  1:2389857/149‑1 (MQ=255)
                                                                                                                        tAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATccc                            <  2:3479951/149‑1 (MQ=255)
                                                                                                                         aGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCg                           >  2:72383/1‑149 (MQ=255)
                                                                                                                          ggCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCgg                          <  2:4453141/149‑1 (MQ=255)
                                                                                                                          ggCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCgg                          >  1:2110594/1‑149 (MQ=255)
                                                                                                                          ggCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCgg                          >  2:3174159/1‑149 (MQ=255)
                                                                                                                              ggCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAtt                      <  1:932661/149‑1 (MQ=255)
                                                                                                                                cGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTaa                    >  1:2286825/1‑149 (MQ=255)
                                                                                                                                  aagaaCCGCTCACACCTACCACCGGGGGGCGCCTCGGCTCAGCAATTCCTTCCGCTTCGCCCAAGCAGCGGACAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGGTTGGCGTTCCGGAGGTCATCCCGGCAGTCAcc                  >  2:142810/1‑149 (MQ=255)
                                                                                                                                   agaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCa                 >  1:4706419/1‑149 (MQ=255)
                                                                                                                                         gCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCgg           >  1:3527835/1‑149 (MQ=255)
                                                                                                                                         gCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCgg           <  1:2205148/149‑1 (MQ=255)
                                                                                                                                            cacaCCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg        <  1:1264768/149‑1 (MQ=255)
                                                                                                                                             acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgt       <  2:2525314/149‑1 (MQ=255)
                                                                                                                                              cacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgtg      >  1:3051278/1‑149 (MQ=255)
                                                                                                                                              cacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgtg      <  1:1867301/149‑1 (MQ=255)
                                                                                                                                              cacCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCTGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgtg      >  1:277590/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCG  >  CP009273/400448‑400742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑