Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 411,027 G→T 100% L61L (CTC→CTA)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273411,0270GT99.2% 909.9 / ‑1.7 275L61L (CTC→CTA) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (137/135);  total (140/135)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

GCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATG  >  CP009273/410882‑411172
                                                                                                                                                 |                                                                                                                                                 
gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTtagataga                                                                                                                                                <  2:4000675/149‑1 (MQ=255)
gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTtagataga                                                                                                                                                <  2:1613539/149‑1 (MQ=255)
gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTtagataga                                                                                                                                                <  2:1272720/149‑1 (MQ=255)
 cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAc                                                                                                                                               <  2:3730379/149‑1 (MQ=255)
       aCCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAg                                                                                                                                         <  1:1117855/149‑1 (MQ=255)
        ccGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGt                                                                                                                                        <  1:4190326/149‑1 (MQ=255)
          gTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGttt                                                                                                                                      <  1:899832/149‑1 (MQ=255)
           tCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTc                                                                                                                                     >  1:3305648/1‑149 (MQ=255)
              ggttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                  >  2:3833803/1‑149 (MQ=255)
              ggttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                  <  2:2579799/149‑1 (MQ=255)
                ttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCCCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGAt                                                                                                                                <  2:4183126/149‑1 (MQ=255)
                        gcgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGcc                                                                                                                        >  1:2370737/1‑149 (MQ=255)
                         cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                       <  2:1451513/149‑1 (MQ=255)
                         cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                       <  2:3840780/149‑1 (MQ=255)
                          gcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                      >  1:3112558/1‑149 (MQ=255)
                            ccGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGAc                                                                                                                    >  1:1555874/1‑149 (MQ=255)
                            ccGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGAc                                                                                                                    >  1:3945874/1‑149 (MQ=255)
                             cGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACa                                                                                                                   >  2:1113079/1‑149 (MQ=255)
                              gATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                  >  1:2850508/1‑149 (MQ=255)
                               aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaaa                                                                                                                 >  2:745257/1‑149 (MQ=255)
                               aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaaa                                                                                                                 >  1:123648/1‑149 (MQ=255)
                               aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaaa                                                                                                                 <  1:1535984/149‑1 (MQ=255)
                                ttCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                                >  2:3288456/1‑149 (MQ=255)
                                   tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTCGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                             >  2:2628579/1‑149 (MQ=255)
                                   tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                             >  2:2499603/1‑149 (MQ=255)
                                   tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                             <  1:1061904/149‑1 (MQ=255)
                                   tGGCTCCAGAATGCACGGTACGCTTCACCCTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                             <  1:2254434/149‑1 (MQ=255)
                                      cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc                                                                                                          <  1:4278472/149‑1 (MQ=255)
                                             aTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAgg                                                                                                   >  1:4446840/1‑149 (MQ=255)
                                               gCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggt                                                                                                 <  1:2170877/149‑1 (MQ=255)
                                                   ggTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtt                                                                                             >  2:1781064/1‑149 (MQ=255)
                                                    gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                            <  2:3004845/149‑1 (MQ=255)
                                                    gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                            <  1:294607/149‑1 (MQ=255)
                                                           tCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGc                                                                                     >  2:3483543/1‑149 (MQ=255)
                                                              ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCAcc                                                                                  <  2:2381908/149‑1 (MQ=255)
                                                              ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCAcc                                                                                  <  1:302747/149‑1 (MQ=255)
                                                               cTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                 <  2:3644986/149‑1 (MQ=255)
                                                                   cACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTg                                                                             >  1:2003630/1‑149 (MQ=255)
                                                                     cTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTggg                                                                           <  2:612452/149‑1 (MQ=255)
                                                                         aaaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCgg                                                                       <  1:3674504/149‑1 (MQ=255)
                                                                          aaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                      >  1:3290567/1‑149 (MQ=255)
                                                                          aaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                      >  1:702508/1‑149 (MQ=255)
                                                                            cTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTaa                                                                    <  1:407067/149‑1 (MQ=255)
                                                                              ccACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATa                                                                  >  2:4360568/1‑149 (MQ=255)
                                                                                aCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                >  1:4119288/1‑149 (MQ=255)
                                                                                aCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                >  1:3806662/1‑149 (MQ=255)
                                                                                  cTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaa                                                              >  1:1369648/1‑149 (MQ=255)
                                                                                    ccGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAc                                                            <  1:3466233/149‑1 (MQ=255)
                                                                                     cGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACa                                                           <  1:3427864/149‑1 (MQ=255)
                                                                                      gCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAg                                                          <  2:1903878/149‑1 (MQ=255)
                                                                                         aGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                       <  2:3408455/149‑1 (MQ=255)
                                                                                           aCATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGt                                                     >  2:3915165/1‑149 (MQ=255)
                                                                                               tCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                 <  2:4248978/149‑1 (MQ=255)
                                                                                                cggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTg                                                <  2:2393838/149‑1 (MQ=255)
                                                                                                  gcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                              >  2:4383630/1‑149 (MQ=255)
                                                                                                  gcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                              >  1:439578/1‑149 (MQ=255)
                                                                                                  gcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                              >  2:1994078/1‑149 (MQ=255)
                                                                                                     gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                           <  2:1998059/149‑1 (MQ=255)
                                                                                                     gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                           <  2:609082/149‑1 (MQ=255)
                                                                                                     gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                           >  2:1690120/1‑149 (MQ=255)
                                                                                                       aTCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACg                                         >  2:3074650/1‑149 (MQ=255)
                                                                                                        tCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGAAACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACgg                                        >  1:2563365/1‑149 (MQ=255)
                                                                                                          gcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCt                                      <  2:4208899/149‑1 (MQ=255)
                                                                                                          gcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCt                                      <  1:884664/149‑1 (MQ=255)
                                                                                                           cgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTc                                     <  1:4292061/149‑1 (MQ=255)
                                                                                                            gggTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcg                                    >  1:1862648/1‑149 (MQ=255)
                                                                                                                   gagaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                             >  2:57104/1‑149 (MQ=255)
                                                                                                                   gagaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                             >  2:1731388/1‑149 (MQ=255)
                                                                                                                   gagaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                             >  2:480056/1‑149 (MQ=255)
                                                                                                                     gaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaaa                           <  2:4738551/149‑1 (MQ=255)
                                                                                                                     gaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaaa                           >  2:877015/1‑149 (MQ=255)
                                                                                                                      aTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaat                          >  1:689348/1‑149 (MQ=255)
                                                                                                                       tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatc                         >  2:773665/1‑149 (MQ=255)
                                                                                                                       tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatc                         >  2:2706189/1‑149 (MQ=255)
                                                                                                                       tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatc                         >  2:4402427/1‑149 (MQ=255)
                                                                                                                       tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatc                         <  1:2656325/149‑1 (MQ=255)
                                                                                                                         aTTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACCGCGCCCGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaa                       <  2:1301849/149‑1 (MQ=255)
                                                                                                                          ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaat                      <  1:3346168/149‑1 (MQ=255)
                                                                                                                          ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaat                      <  1:3664349/149‑1 (MQ=255)
                                                                                                                           tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                     >  1:2613789/1‑149 (MQ=255)
                                                                                                                           tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                     >  1:2237294/1‑149 (MQ=255)
                                                                                                                              gCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACCGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  <  2:963362/149‑1 (MQ=255)
                                                                                                                              gCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  <  2:1405045/149‑1 (MQ=255)
                                                                                                                              gCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  <  1:4546012/149‑1 (MQ=255)
                                                                                                                               cGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTcc                 <  2:2162861/149‑1 (MQ=255)
                                                                                                                                gATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCa                <  2:4079886/149‑1 (MQ=255)
                                                                                                                                gATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCa                <  1:4497211/149‑1 (MQ=255)
                                                                                                                                gATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAAGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGATCGCGGGTGAAATCAATCTTCCa                >  1:3654474/1‑149 (MQ=255)
                                                                                                                                 aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt               <  1:1461726/149‑1 (MQ=255)
                                                                                                                                 aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt               >  2:4524581/1‑149 (MQ=255)
                                                                                                                                   tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc             <  2:2734392/149‑1 (MQ=255)
                                                                                                                                      gAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGcc          >  2:3138071/1‑149 (MQ=255)
                                                                                                                                         aCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGACATCAATCTTCCATTCGCCttt       >  2:3713711/1‑149 (MQ=255)
                                                                                                                                          cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt      <  1:4613428/149‑1 (MQ=255)
                                                                                                                                          cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt      >  2:518343/1‑149 (MQ=255)
                                                                                                                                          cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt      <  2:1824879/149‑1 (MQ=255)
                                                                                                                                          cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt      <  1:437434/149‑1 (MQ=255)
                                                                                                                                          cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt      >  1:116922/1‑149 (MQ=255)
                                                                                                                                            ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa    >  2:2950831/1‑149 (MQ=255)
                                                                                                                                             tagatagaCGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt   <  2:25515/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATG  >  CP009273/410882‑411172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑