Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 425,143 G→T 100% T61T (ACG→ACT)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273425,1430GT98.8% 883.3 / 0.6 260T61T (ACG→ACT) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (118/139);  total (121/139)
Fisher's exact test for biased strand distribution p-value = 9.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 265 total aligned reads displayed.

GGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGT  >  CP009273/424997‑425288
                                                                                                                                                  |                                                                                                                                                 
ggCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTActct                                                                                                                                                 >  2:953272/1‑149 (MQ=255)
ggCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTActct                                                                                                                                                 >  2:655899/1‑149 (MQ=255)
      aaaGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGaaaa                                                                                                                                           >  2:511422/1‑149 (MQ=255)
        aGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAAc                                                                                                                                         >  1:988973/1‑149 (MQ=255)
        aGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAAc                                                                                                                                         <  1:1967963/149‑1 (MQ=255)
         gTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAAcc                                                                                                                                        >  1:3000859/1‑149 (MQ=255)
         gTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAAcc                                                                                                                                        <  1:2512942/149‑1 (MQ=255)
           cTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCgg                                                                                                                                      <  1:3672205/149‑1 (MQ=255)
             aTGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCt                                                                                                                                    <  2:4484863/149‑1 (MQ=255)
                aCTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGaa                                                                                                                                 >  2:3452694/1‑149 (MQ=255)
                  tttATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAAt                                                                                                                               >  1:4228971/1‑149 (MQ=255)
                      tGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGAc                                                                                                                           >  1:452817/1‑149 (MQ=255)
                         gcTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTa                                                                                                                        <  2:1260943/149‑1 (MQ=255)
                         gcTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTa                                                                                                                        <  2:2576496/149‑1 (MQ=255)
                         gcTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTa                                                                                                                        <  2:3650669/149‑1 (MQ=255)
                          cTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTaa                                                                                                                       <  2:3828908/149‑1 (MQ=255)
                          cTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTaa                                                                                                                       <  2:301028/149‑1 (MQ=255)
                              gACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCg                                                                                                                   <  2:1171941/149‑1 (MQ=255)
                               actactGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGt                                                                                                                  <  2:4577258/149‑1 (MQ=255)
                               actactGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGGTATTGAAATTACTCTCGACAAACCGGCTGAAATTGACGTAATGCGt                                                                                                                  <  2:2486401/149‑1 (MQ=255)
                               acgactGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGt                                                                                                                  <  2:2251684/146‑1 (MQ=255)
                                  actGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGAt                                                                                                               >  1:2116516/1‑149 (MQ=255)
                                     gggCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCa                                                                                                            >  2:44288/1‑149 (MQ=255)
                                     gggCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCa                                                                                                            <  1:4148801/149‑1 (MQ=255)
                                      ggCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCAt                                                                                                           <  2:672629/149‑1 (MQ=255)
                                       gCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCAtt                                                                                                          <  1:2013549/149‑1 (MQ=255)
                                        cTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTg                                                                                                         >  1:2858685/1‑149 (MQ=255)
                                        cTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTg                                                                                                         <  2:2869772/149‑1 (MQ=255)
                                          ttCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCa                                                                                                       >  2:2737466/1‑149 (MQ=255)
                                          ttCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCa                                                                                                       <  1:780265/149‑1 (MQ=255)
                                             ggCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCaaaa                                                                                                    >  1:3176718/1‑149 (MQ=255)
                                              gCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCaaaaa                                                                                                   <  2:3228237/149‑1 (MQ=255)
                                              gCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCaaaaa                                                                                                   <  1:1484283/149‑1 (MQ=255)
                                              gCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCaaaaa                                                                                                   >  1:2742720/1‑149 (MQ=255)
                                                 tctcTGGTCTGCTGTTACTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCGGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGcc                                                                                                <  1:3643339/149‑1 (MQ=255)
                                                 tctcTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGcc                                                                                                <  1:2916284/149‑1 (MQ=255)
                                                 tctcTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGcc                                                                                                <  1:1859986/149‑1 (MQ=255)
                                                 tctcTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGcc                                                                                                <  1:641388/149‑1 (MQ=255)
                                                  ctctGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCg                                                                                               <  2:792975/149‑1 (MQ=255)
                                                   tctGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCgg                                                                                              <  2:1838210/149‑1 (MQ=255)
                                                    ctGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGt                                                                                             <  2:3647489/149‑1 (MQ=255)
                                                     tGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGtt                                                                                            >  2:3742102/1‑149 (MQ=255)
                                                      ggTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGttt                                                                                           >  1:4440976/1‑149 (MQ=255)
                                                      ggTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGttt                                                                                           >  2:822347/1‑149 (MQ=255)
                                                      ggTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGttt                                                                                           >  1:309186/1‑149 (MQ=255)
                                                       gTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGtttt                                                                                          <  2:2116870/149‑1 (MQ=255)
                                                          tgctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGaa                                                                                       <  2:4410152/149‑1 (MQ=255)
                                                           gctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAag                                                                                      <  2:3122712/149‑1 (MQ=255)
                                                           gctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAag                                                                                      >  1:1305741/1‑149 (MQ=255)
                                                             tgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAagag                                                                                    >  1:2346563/1‑149 (MQ=255)
                                                               ttAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGcc                                                                                  >  2:1621680/1‑149 (MQ=255)
                                                               ttAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGcc                                                                                  <  1:1039439/149‑1 (MQ=255)
                                                                 aaTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGa                                                                                <  2:1398307/149‑1 (MQ=255)
                                                                 aaTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGa                                                                                >  2:918242/1‑149 (MQ=255)
                                                                    cGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGAtgc                                                                             <  1:3143168/149‑1 (MQ=255)
                                                                           aTCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaaaa                                                                      >  1:2294981/1‑149 (MQ=255)
                                                                            tCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAAc                                                                     <  1:244735/149‑1 (MQ=255)
                                                                            tCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAAc                                                                     <  2:1247711/149‑1 (MQ=255)
                                                                               ttattaTGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACttt                                                                  <  2:607978/149‑1 (MQ=255)
                                                                               ttattaTGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACttt                                                                  >  1:3959460/1‑149 (MQ=255)
                                                                                 attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTgg                                                                >  1:3402239/1‑149 (MQ=255)
                                                                                 attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTgg                                                                <  2:2110744/149‑1 (MQ=255)
                                                                                  ttatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTCCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGt                                                               <  1:1290871/149‑1 (MQ=255)
                                                                                   tatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTa                                                              <  1:1732561/149‑1 (MQ=255)
                                                                                     tGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTagc                                                            <  1:2365796/149‑1 (MQ=255)
                                                                                         cGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGcc                                                        <  2:876104/149‑1 (MQ=255)
                                                                                           tGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCAt                                                      >  1:2527608/1‑149 (MQ=255)
                                                                                             cgcgGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGa                                                    <  1:2836371/149‑1 (MQ=255)
                                                                                              gcgGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAc                                                   <  2:294688/149‑1 (MQ=255)
                                                                                                ggCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcat                                                 >  2:516866/1‑149 (MQ=255)
                                                                                                 gCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc                                                <  1:1031675/149‑1 (MQ=255)
                                                                                                 gCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc                                                >  2:868387/1‑149 (MQ=255)
                                                                                                 gCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc                                                >  1:262484/1‑149 (MQ=255)
                                                                                                  cTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatca                                               >  1:504321/1‑149 (MQ=255)
                                                                                                  cTTTAAATGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatca                                               >  1:850992/1‑149 (MQ=255)
                                                                                                    ttAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATg                                             >  1:753959/1‑149 (MQ=255)
                                                                                                      aaCTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGt                                           >  1:3359590/1‑149 (MQ=255)
                                                                                                       aCTTGGGGCTGGATTTCACCGGTTGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATTCTTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATTCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTc                                          <  2:2064479/149‑1 (MQ=255)
                                                                                                       aCTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTc                                          <  2:3091229/149‑1 (MQ=255)
                                                                                                        cTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTcc                                         >  2:3874178/1‑149 (MQ=255)
                                                                                                         tGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg                                        <  1:4535923/149‑1 (MQ=255)
                                                                                                          gggggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGt                                       >  1:4671828/1‑149 (MQ=255)
                                                                                                              gCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgc                                   >  2:4203406/1‑149 (MQ=255)
                                                                                                              gCTGGATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgc                                   >  2:1813043/1‑149 (MQ=255)
                                                                                                                 ggATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgccgc                                >  1:3605287/1‑149 (MQ=255)
                                                                                                                 ggATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgccgc                                <  2:2332822/149‑1 (MQ=255)
                                                                                                                  gATTTCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgccgcc                               >  1:1827403/1‑149 (MQ=255)
                                                                                                                    tttCACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCctg                             >  2:1008803/1‑149 (MQ=255)
                                                                                                                       cACCGGTGGTACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCctgctg                          >  1:3556226/1‑149 (MQ=255)
                                                                                                                           ggtggtACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAgg                      <  2:432883/149‑1 (MQ=255)
                                                                                                                            gtggtACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGc                     >  1:1512651/1‑149 (MQ=255)
                                                                                                                            gtggtACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGc                     <  1:3895234/149‑1 (MQ=255)
                                                                                                                                tACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGaaa                 >  2:2401367/1‑149 (MQ=255)
                                                                                                                                tACGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGaaa                 <  2:985597/149‑1 (MQ=255)
                                                                                                                                 aCGGTTATTGAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAAc                <  2:1785509/149‑1 (MQ=255)
                                                                                                                                   ggTTATTTATATTACTCTCGATAAACCGGCTGAAATTGACGTAATGCGTGATGAATTGCTAAAAGCCGGTTTTGTAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACcg              <  1:4385642/149‑1 (MQ=255)
                                                                                                                                          gAAATTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCa       <  2:2674057/149‑1 (MQ=255)
                                                                                                                                            aaTTACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAgg     <  1:13610/149‑1 (MQ=255)
                                                                                                                                              ttACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGgtg   >  2:1694898/1‑149 (MQ=255)
                                                                                                                                               tACTCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGgtgt  <  2:3402882/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGT  >  CP009273/424997‑425288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑