Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,862 A→T 85.2% T232T (ACT→ACA)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,8620AT85.2% 606.1 / 97.2 256T232T (ACT→ACA) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (21/17);  new base T (114/102);  total (137/119)
Fisher's exact test for biased strand distribution p-value = 8.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-02

Only 100 of 258 total aligned reads displayed.

GACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGC  >  CP009273/435716‑436010
                                                                                                                                                  |                                                                                                                                                    
gACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGt                                                                                                                                                    <  2:1227186/149‑1 (MQ=255)
gACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGt                                                                                                                                                    <  2:3615741/149‑1 (MQ=255)
    gTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAtct                                                                                                                                                <  2:1582147/149‑1 (MQ=255)
     ttCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAtctc                                                                                                                                               <  1:2187871/149‑1 (MQ=255)
       cAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGAACCCGGGCTTTCTTCCGGGCTTGCTCAATACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAAAGTTGAAGTATCTacc                                                                                                                                             >  1:196829/1‑149 (MQ=255)
           cAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCccacca                                                                                                                                         <  2:2733180/149‑1 (MQ=255)
           cAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCccacca                                                                                                                                         <  2:3684637/149‑1 (MQ=255)
            aGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCcaccac                                                                                                                                        <  1:423893/149‑1 (MQ=255)
             gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACcaca                                                                                                                                       >  1:2671235/1‑149 (MQ=255)
             gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACcaca                                                                                                                                       <  2:735747/149‑1 (MQ=255)
             gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTCCCACcaca                                                                                                                                       >  1:795148/1‑149 (MQ=255)
             gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAACGTTGCTGTATCTCCCACcaca                                                                                                                                       >  1:2975308/1‑149 (MQ=255)
                gttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATcc                                                                                                                                    <  1:1575636/149‑1 (MQ=255)
                gttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATcc                                                                                                                                    <  1:3831205/149‑1 (MQ=255)
                 tttCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCa                                                                                                                                   <  2:3341107/149‑1 (MQ=255)
                     aGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGAt                                                                                                                               >  1:4629066/1‑149 (MQ=255)
                     aGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAAAGGTTCTGTATCTCCCACCCCAACCAGGAt                                                                                                                               >  1:1380209/1‑149 (MQ=255)
                           tGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATc                                                                                                                         >  2:1872900/1‑149 (MQ=255)
                           tGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATc                                                                                                                         >  2:4674093/1‑149 (MQ=255)
                            gACGGGCATCGTCGATCCGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCGGTATCTACCACCACAACCAGGATGTGAtac                                                                                                                        >  1:3311398/1‑147 (MQ=255)
                            gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                        >  1:4319038/1‑149 (MQ=255)
                            gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                        >  1:1103487/1‑149 (MQ=255)
                            gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATATCCCACCACATCCCGGATGTCATcc                                                                                                                        >  1:1929138/1‑149 (MQ=255)
                            gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTCCCACCACAACCAGGAAGTCATcc                                                                                                                        >  1:2128466/1‑149 (MQ=255)
                                 gCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAc                                                                                                                   >  2:2118825/1‑149 (MQ=255)
                                   aTCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCt                                                                                                                 >  2:2901861/1‑149 (MQ=255)
                                    tcgtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTg                                                                                                                <  2:1596219/149‑1 (MQ=255)
                                      gtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGa                                                                                                              <  1:1672554/149‑1 (MQ=255)
                                       tcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                                                                             <  2:3557321/149‑1 (MQ=255)
                                          aTCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggc                                                                                                          <  2:2514571/149‑1 (MQ=255)
                                          aTCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggc                                                                                                          >  2:4647134/1‑149 (MQ=255)
                                            cAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCTACCACCACATACAGGATGTCATACAGAACCTGGaaggcaa                                                                                                        >  1:2241721/1‑149 (MQ=255)
                                            cAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaa                                                                                                        <  1:1030981/149‑1 (MQ=255)
                                            cAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATATCCCACCACAACCACGATGTAATCCAGAAACTTGaaggcaa                                                                                                        >  1:149739/1‑149 (MQ=255)
                                               aTCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaaggc                                                                                                     <  2:1694135/149‑1 (MQ=255)
                                                tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGcc                                                                                                    >  2:3405814/1‑149 (MQ=255)
                                                tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGcc                                                                                                    >  2:1016512/1‑149 (MQ=255)
                                                  ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGa                                                                                                  <  2:4741912/149‑1 (MQ=255)
                                                  ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGa                                                                                                  <  1:4377888/149‑1 (MQ=255)
                                                    gggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATg                                                                                                <  1:667429/149‑1 (MQ=255)
                                                     ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGc                                                                                               >  2:2349488/1‑149 (MQ=255)
                                                        tttCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctct                                                                                            >  2:3041265/1‑149 (MQ=255)
                                                           cttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTg                                                                                         >  1:3459848/1‑149 (MQ=255)
                                                            ttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGGTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTTTCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTGTCTGc                                                                                        <  2:2058673/149‑1 (MQ=255)
                                                              ccGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCAt                                                                                      <  2:947902/149‑1 (MQ=255)
                                                                 ggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACt                                                                                   <  2:4629023/149‑1 (MQ=255)
                                                                 ggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACt                                                                                   <  2:1506264/149‑1 (MQ=255)
                                                                    ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGCAGGCAAGGCCGATGCTCTCTGCATACgtgt                                                                                >  2:1196110/1‑149 (MQ=255)
                                                                        tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAg                                                                            <  1:3252718/149‑1 (MQ=255)
                                                                        tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAg                                                                            <  2:3505362/149‑1 (MQ=255)
                                                                         cAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGt                                                                           <  1:2516819/149‑1 (MQ=255)
                                                                          aaGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACATGTCGAGTa                                                                          >  2:4022068/1‑149 (MQ=255)
                                                                          aaGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCCAGGCCGATGCTCTCTGCATACTTGTCGAGTa                                                                          >  1:2609258/1‑149 (MQ=255)
                                                                             aCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                       <  1:887971/149‑1 (MQ=255)
                                                                             aCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                       >  2:180166/1‑149 (MQ=255)
                                                                              cccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCgg                                                                      >  1:3185886/1‑149 (MQ=255)
                                                                                  gAAGTGCAGGGTAGGTACTTTTACCAAGGTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCaga                                                                  <  2:2036337/149‑1 (MQ=255)
                                                                                           ggtaggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGt                                                         <  1:4647134/149‑1 (MQ=255)
                                                                                              aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTTCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGCTCTATGCATACTTGTCGAGTACCGGCAGATCACGACGTCCt                                                      >  2:277610/1‑149 (MQ=255)
                                                                                              aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTTCTGTATCTCCCACCACATACAGGATGTCATCCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGCAAACTTTTCGAGTAACGGCAGAGCACGACGTCCt                                                      >  1:1990181/1‑149 (MQ=255)
                                                                                              aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                      <  1:4060256/149‑1 (MQ=255)
                                                                                              aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCAACCAGAACCAGGAAGGCAAGGCCGAAGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                      >  1:1990350/1‑149 (MQ=255)
                                                                                               ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATATCCCACCACATCCAGGATGTGATCCTGAACCTGGAAGGCCAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                     >  2:1638531/1‑149 (MQ=255)
                                                                                                gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAAACTTTCTGTATCTCCCACCACAACCAGGATGTCAACCAGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACCACGTCCttt                                                    >  1:3682229/1‑149 (MQ=255)
                                                                                                gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                    <  1:923098/149‑1 (MQ=255)
                                                                                                 taCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                   <  1:2476040/149‑1 (MQ=255)
                                                                                                   cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGCCGGCCTGTAtc                                                 >  2:4133072/1‑149 (MQ=255)
                                                                                                   cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtc                                                 >  2:4172690/1‑149 (MQ=255)
                                                                                                    ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCAGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                                >  1:4745343/1‑149 (MQ=255)
                                                                                                    ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTTCTTTATCACCCACCACAAACACGATGTCATCCTGAACCAGGAATGCAAGGCCGATTCTATCTGCATACTTTTAGAGTACCCGCAGAGCACGACGTCCTTTAtat                                                >  1:2067952/1‑147 (MQ=255)
                                                                                                      ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                              <  1:1077277/149‑1 (MQ=255)
                                                                                                        aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGTAGGCAAGGCCGATGCTCTCTGGATACTTGTCGAGTACCGGCAGAGCACGACGTCCGTTATCTCCgg                                            >  1:4166430/1‑149 (MQ=255)
                                                                                                        aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                            <  1:4420642/149‑1 (MQ=255)
                                                                                                         ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACCTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                           <  2:1380209/149‑1 (MQ=255)
                                                                                                         ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                           <  1:3384030/149‑1 (MQ=255)
                                                                                                          cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcg                                          >  2:1496500/1‑149 (MQ=255)
                                                                                                          cAAGTTGCTGGTCGGCACCCTGGCGTGTTCCCAACGTTTCTGTATCTCCCACCACAACCACGATGTCAACCTGAACCAGGAAGGCAAGGCCGATGCTCTATGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcg                                          >  1:1295658/1‑149 (MQ=255)
                                                                                                           aaGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcgc                                         <  2:4605883/149‑1 (MQ=255)
                                                                                                           aaGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcgc                                         <  2:3010042/149‑1 (MQ=255)
                                                                                                             gTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCCTACTTGTCGGGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                       >  2:2881225/1‑149 (MQ=255)
                                                                                                              ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCTGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                      <  1:1923768/149‑1 (MQ=255)
                                                                                                                gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                    <  2:4351879/149‑1 (MQ=255)
                                                                                                                   ggTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                 >  2:779235/1‑149 (MQ=255)
                                                                                                                    gTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCAc                                <  2:2367584/149‑1 (MQ=255)
                                                                                                                        gCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAg                            <  1:4674093/149‑1 (MQ=255)
                                                                                                                          aCCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                          <  2:221938/149‑1 (MQ=255)
                                                                                                                             cTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                       >  1:279920/1‑149 (MQ=255)
                                                                                                                             cTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                       >  1:1755338/1‑149 (MQ=255)
                                                                                                                               ggCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                     >  2:2290042/1‑149 (MQ=255)
                                                                                                                                 cGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggc                   <  1:2872850/149‑1 (MQ=255)
                                                                                                                                  gTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg                  >  2:3812061/1‑149 (MQ=255)
                                                                                                                                   ttttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCCGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCCAGGCGAAcggcgg                 >  2:1559081/1‑149 (MQ=255)
                                                                                                                                       cccAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGcgcg             >  1:4298667/1‑149 (MQ=255)
                                                                                                                                       cccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCTAACGGCGGcgcg             >  1:4287176/1‑149 (MQ=255)
                                                                                                                                        ccAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGa            >  1:4486536/1‑149 (MQ=255)
                                                                                                                                         cAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGaa           <  1:1222305/149‑1 (MQ=255)
                                                                                                                                             gTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa       >  1:2927754/1‑149 (MQ=255)
                                                                                                                                              ttGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt      <  2:4279164/149‑1 (MQ=255)
                                                                                                                                               tGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg     <  1:2118787/149‑1 (MQ=255)
                                                                                                                                                  aGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGACCGGCGGCGCGAATCAATgcgc  >  1:1758111/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGC  >  CP009273/435716‑436010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑