Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,672 T→A 100% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,6720TA99.6% 793.5 / ‑5.0 240V223E (GTG→GAG) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (125/113);  total (126/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

GGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCC  >  CP009273/437527‑437817
                                                                                                                                                 |                                                                                                                                                 
ggCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGgc                                                                                                                                                >  1:170477/1‑149 (MQ=255)
 gCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGgcg                                                                                                                                               >  2:727761/1‑149 (MQ=255)
 gCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGgcg                                                                                                                                               >  1:3131814/1‑149 (MQ=255)
    cccAGGAAGATGTGCTGTCGCTCATTTACGGTGGTTTCGACTCCGGTGTTTGCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAt                                                                                                                                            >  2:1624058/1‑149 (MQ=255)
     ccAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCTGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAtt                                                                                                                                           <  2:3051099/149‑1 (MQ=255)
      cAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTa                                                                                                                                          >  2:746584/1‑149 (MQ=255)
       aGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAt                                                                                                                                         >  1:647881/1‑149 (MQ=255)
        ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                        <  1:2626359/149‑1 (MQ=255)
        ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                        >  2:2026163/1‑149 (MQ=255)
        ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                        <  1:1929318/149‑1 (MQ=255)
        ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                        >  2:2944219/1‑149 (MQ=255)
         gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCt                                                                                                                                       <  2:378144/149‑1 (MQ=255)
         gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGAGCATTACTGCTTCTTTAACCTCGGCGGCGCAGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCt                                                                                                                                       >  2:3577165/1‑149 (MQ=255)
           aGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtgt                                                                                                                                     <  2:3484518/149‑1 (MQ=255)
            gATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtgtg                                                                                                                                    <  1:752161/149‑1 (MQ=255)
              tgtgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGa                                                                                                                                  >  2:3361370/1‑149 (MQ=255)
                 gCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTTGCGTGCGTCAGGAGGCGCAATATCTGTGGAAcc                                                                                                                               >  2:2828072/1‑149 (MQ=255)
                     tCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                           <  1:4690435/149‑1 (MQ=255)
                       gCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTg                                                                                                                         <  1:1437364/149‑1 (MQ=255)
                       gCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTg                                                                                                                         >  2:2561931/1‑149 (MQ=255)
                        cTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                        <  2:2991798/149‑1 (MQ=255)
                        cTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATGACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                        <  2:3924302/149‑1 (MQ=255)
                         tCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGc                                                                                                                       <  1:1811471/149‑1 (MQ=255)
                          cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCa                                                                                                                      <  2:4276598/149‑1 (MQ=255)
                          cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCa                                                                                                                      >  2:3391989/1‑149 (MQ=255)
                           aTTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAg                                                                                                                     >  1:1075337/1‑149 (MQ=255)
                           aTTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAg                                                                                                                     >  2:630354/1‑149 (MQ=255)
                               ccGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTcc                                                                                                                 >  1:1835487/1‑149 (MQ=255)
                                  gtggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAc                                                                                                              >  1:1243675/1‑149 (MQ=255)
                                      tttCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACcgcg                                                                                                          <  1:25541/149‑1 (MQ=255)
                                         cGACTCCTGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgc                                                                                                       >  2:553105/1‑149 (MQ=255)
                                         cGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgc                                                                                                       <  1:3433540/149‑1 (MQ=255)
                                            cTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtt                                                                                                    <  1:2000642/149‑1 (MQ=255)
                                             tCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGttt                                                                                                   <  1:4217826/149‑1 (MQ=255)
                                              ccGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtttt                                                                                                  <  2:1288323/149‑1 (MQ=255)
                                                 gtgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTc                                                                                               <  1:2362/149‑1 (MQ=255)
                                                 gtgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTc                                                                                               >  2:158459/1‑149 (MQ=255)
                                                  tgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGTAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                              <  2:2015841/149‑1 (MQ=255)
                                                  tgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                              >  2:2850691/1‑149 (MQ=255)
                                                    tttCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCt                                                                                            >  2:3284995/1‑149 (MQ=255)
                                                       ccAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAtt                                                                                         >  1:179372/1‑149 (MQ=255)
                                                        cAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTa                                                                                        <  1:3496354/149‑1 (MQ=255)
                                                          gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                      <  1:2272969/149‑1 (MQ=255)
                                                           ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAtt                                                                                     <  1:4242784/149‑1 (MQ=255)
                                                           ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAtt                                                                                     >  1:4382730/1‑149 (MQ=255)
                                                           ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAtt                                                                                     >  1:4382788/1‑149 (MQ=255)
                                                            tataTGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                    >  1:2560433/1‑149 (MQ=255)
                                                             atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTc                                                                                   <  2:1666455/149‑1 (MQ=255)
                                                             atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTc                                                                                   >  1:4005513/1‑149 (MQ=255)
                                                                tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                                >  1:2211313/1‑149 (MQ=255)
                                                                tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                                >  1:2211083/1‑149 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAc                                                                               <  1:561188/149‑1 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAc                                                                               <  1:354706/149‑1 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAc                                                                               >  2:261590/1‑149 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAc                                                                               <  2:2409787/149‑1 (MQ=255)
                                                                     aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgt                                                                           >  1:4571208/1‑149 (MQ=255)
                                                                          tCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGtcgtcg                                                                      >  1:1379401/1‑149 (MQ=255)
                                                                          tCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGtcgtcg                                                                      >  1:3608898/1‑149 (MQ=255)
                                                                           cgcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgg                                                                     <  1:2892693/149‑1 (MQ=255)
                                                                             cgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgggg                                                                   <  2:264953/149‑1 (MQ=255)
                                                                             cgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgggg                                                                   <  2:2248281/149‑1 (MQ=255)
                                                                                     gcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACATtctc                                                           >  2:1486905/1‑149 (MQ=255)
                                                                                     gcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtctc                                                           >  2:2345680/1‑149 (MQ=255)
                                                                                        tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgag                                                        >  2:699404/1‑149 (MQ=255)
                                                                                            ttACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaaa                                                    >  1:4519567/1‑149 (MQ=255)
                                                                                            ttACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaaa                                                    >  1:2771305/1‑149 (MQ=255)
                                                                                             tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAt                                                   <  1:3046206/149‑1 (MQ=255)
                                                                                              aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATc                                                  <  1:1596939/149‑1 (MQ=255)
                                                                                                tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAACTTCTCGAGAAAATcga                                                >  2:3727768/1‑149 (MQ=255)
                                                                                                tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcga                                                <  1:2128867/149‑1 (MQ=255)
                                                                                                 gCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgac                                               <  2:1151447/149‑1 (MQ=255)
                                                                                                   ttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                             >  1:2157833/1‑149 (MQ=255)
                                                                                                    tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCgacgac                                            >  1:319681/1‑149 (MQ=255)
                                                                                                        tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTc                                        <  2:418660/149‑1 (MQ=255)
                                                                                                          aCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAg                                      >  2:1088802/1‑149 (MQ=255)
                                                                                                           ccTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGa                                     >  2:3650161/1‑149 (MQ=255)
                                                                                                            cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                    >  1:4152283/1‑149 (MQ=255)
                                                                                                            cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                    >  2:3061436/1‑149 (MQ=255)
                                                                                                            cTCGGCGGCGCGGCGCATGAAATTGGCGAGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                    >  1:369961/1‑149 (MQ=255)
                                                                                                               ggcggcGCGGCGCTTGAATTTGGCGTGCGTCAGGAGGCGCTTTATCTGGGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGGCGTCGGGTAAATTCTCGAGAAAATCGACGACGGTCAGATggg                                 <  1:3216816/149‑1 (MQ=255)
                                                                                                               ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGGGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGGCGCTATTAATTTCGACCCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATggg                                 <  1:3391559/149‑1 (MQ=255)
                                                                                                               ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAGCGGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATggg                                 <  1:1002602/149‑1 (MQ=255)
                                                                                                                gcggcgcggcGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGc                                <  2:4310796/149‑1 (MQ=255)
                                                                                                                  ggcgcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGt                              <  1:3038303/149‑1 (MQ=255)
                                                                                                                   gcgcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                             <  2:522764/149‑1 (MQ=255)
                                                                                                                     gcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAt                           >  1:1997726/1‑149 (MQ=255)
                                                                                                                      cggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATc                          >  2:1234024/1‑149 (MQ=255)
                                                                                                                       ggcgcATGAAATGGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATcc                         <  2:4533861/149‑1 (MQ=255)
                                                                                                                           cATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                     >  1:2166906/1‑149 (MQ=255)
                                                                                                                             tGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAc                   >  2:2089278/1‑149 (MQ=255)
                                                                                                                             tGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAc                   >  2:1755657/1‑149 (MQ=255)
                                                                                                                                aaTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa                <  2:2127550/149‑1 (MQ=255)
                                                                                                                                    ggCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgat            <  1:1919884/149‑1 (MQ=255)
                                                                                                                                       gtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgt         >  2:469715/1‑149 (MQ=255)
                                                                                                                                       gtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgt         >  1:4754942/1‑149 (MQ=255)
                                                                                                                                        tgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTNGCAGCTCCCACCGNGTGCGTTTTGTCGNTATTAATNTCNAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtg        <  1:4670943/149‑1 (MQ=255)
                                                                                                                                           gtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgt     <  1:2911944/149‑1 (MQ=255)
                                                                                                                                           gtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgt     <  1:3820552/149‑1 (MQ=255)
                                                                                                                                             cAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgtgc   >  1:2342562/1‑149 (MQ=255)
                                                                                                                                              aGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGTAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGcc  <  1:4067201/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCC  >  CP009273/437527‑437817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑