Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 446,369 T→G 100% Q233P (CAG→CCG)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273446,3690TG99.9% 1269.5 / ‑6.6 309Q233P (CAG→CCG) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (142/166);  total (142/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 311 total aligned reads displayed.

TAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCAT  >  CP009273/446223‑446516
                                                                                                                                                  |                                                                                                                                                   
tAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACggg                                                                                                                                                   >  2:243231/1‑149 (MQ=255)
 aaaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGt                                                                                                                                                  >  2:568488/1‑149 (MQ=255)
 aaaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGt                                                                                                                                                  >  2:2640166/1‑149 (MQ=255)
  aaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTc                                                                                                                                                 <  2:249674/149‑1 (MQ=255)
  aaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTc                                                                                                                                                 >  2:4279723/1‑149 (MQ=255)
   aCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCg                                                                                                                                                >  2:2590156/1‑149 (MQ=255)
    cTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGa                                                                                                                                               >  1:1562941/1‑149 (MQ=255)
     ttGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                              <  1:2745549/149‑1 (MQ=255)
     ttGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                              >  1:350931/1‑149 (MQ=255)
     ttGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                              <  1:4315537/149‑1 (MQ=255)
      tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAAt                                                                                                                                             <  1:4305056/149‑1 (MQ=255)
       gTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATg                                                                                                                                            <  1:2112783/149‑1 (MQ=255)
         tAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcg                                                                                                                                          <  1:743942/149‑1 (MQ=255)
         tAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTGTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcg                                                                                                                                          >  2:2929355/1‑149 (MQ=255)
          aaTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcgg                                                                                                                                         >  1:4476570/1‑149 (MQ=255)
          aaTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcgg                                                                                                                                         >  2:1050956/1‑149 (MQ=255)
                 tCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                                  <  2:3048349/149‑1 (MQ=255)
                  cGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATcc                                                                                                                                 <  2:4202135/149‑1 (MQ=255)
                   ggCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCg                                                                                                                                >  1:2811674/1‑149 (MQ=255)
                    gCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCgg                                                                                                                               >  2:228960/1‑149 (MQ=255)
                        aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGtt                                                                                                                           <  1:3928263/149‑1 (MQ=255)
                        aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGtt                                                                                                                           <  1:1811313/149‑1 (MQ=255)
                          aaGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGc                                                                                                                         <  2:1638726/149‑1 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                       >  1:2063100/1‑149 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                       >  1:630344/1‑149 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                       >  2:4613095/1‑149 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                       >  2:1170846/1‑149 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATAGTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                       <  2:4371045/149‑1 (MQ=255)
                             tCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAAt                                                                                                                      <  1:330500/149‑1 (MQ=255)
                              cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATa                                                                                                                     <  1:3372416/149‑1 (MQ=255)
                              cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAGTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATa                                                                                                                     <  2:3110710/149‑1 (MQ=255)
                               tGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAg                                                                                                                    <  1:1761295/149‑1 (MQ=255)
                                    tCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTg                                                                                                               <  2:3903234/149‑1 (MQ=255)
                                        gatGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACt                                                                                                           >  2:4765375/3‑149 (MQ=255)
                                        gTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGTGCGATCCGGTGTTGCAATAGCTTTGAACt                                                                                                           >  2:2049007/1‑149 (MQ=255)
                                        gTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACt                                                                                                           >  2:501604/1‑149 (MQ=255)
                                         ttGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                          >  2:4491800/1‑149 (MQ=255)
                                          tGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTc                                                                                                         <  2:3735412/149‑1 (MQ=255)
                                            gagaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCAt                                                                                                       <  1:2944471/149‑1 (MQ=255)
                                            gagaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCAt                                                                                                       <  2:3725790/149‑1 (MQ=255)
                                                  tatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                                 >  2:1880128/1‑149 (MQ=255)
                                                   atTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgag                                                                                                >  1:2395091/1‑149 (MQ=255)
                                                   atTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgag                                                                                                >  1:2464304/1‑149 (MQ=255)
                                                     tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGaa                                                                                              <  1:2507420/149‑1 (MQ=255)
                                                         ccTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGccc                                                                                          >  1:4238341/1‑149 (MQ=255)
                                                          cTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCg                                                                                         <  2:4180856/149‑1 (MQ=255)
                                                           tGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCgg                                                                                        >  1:1184964/1‑149 (MQ=255)
                                                            ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                       >  2:4512474/1‑149 (MQ=255)
                                                             gTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                      <  1:2855942/149‑1 (MQ=255)
                                                              ttGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGACGCCCGGGcc                                                                                     >  2:771248/1‑149 (MQ=255)
                                                              ttGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGcc                                                                                     >  2:4146341/1‑149 (MQ=255)
                                                                 tATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCt                                                                                  <  1:2566160/149‑1 (MQ=255)
                                                                 tATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCt                                                                                  >  2:1978937/1‑149 (MQ=255)
                                                                   ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGt                                                                                >  2:803420/1‑149 (MQ=255)
                                                                   ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGt                                                                                <  1:3093067/149‑1 (MQ=255)
                                                                     cGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAg                                                                              >  2:1076535/1‑149 (MQ=255)
                                                                       cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCt                                                                            <  1:1051197/149‑1 (MQ=255)
                                                                         aGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTgg                                                                          <  2:3282971/149‑1 (MQ=255)
                                                                             gcgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGga                                                                      <  2:2141705/149‑1 (MQ=255)
                                                                               ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGgaga                                                                    <  1:3781999/149‑1 (MQ=255)
                                                                                gCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                   >  1:2276007/1‑149 (MQ=255)
                                                                                 ccAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATa                                                                  <  2:2069196/149‑1 (MQ=255)
                                                                                    gTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCg                                                               >  2:4090730/1‑149 (MQ=255)
                                                                                        ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCAt                                                           <  2:1269259/149‑1 (MQ=255)
                                                                                        ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCAt                                                           >  2:4654779/1‑149 (MQ=255)
                                                                                         tCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATa                                                          <  1:1651018/149‑1 (MQ=255)
                                                                                           gAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAg                                                        <  2:4316163/149‑1 (MQ=255)
                                                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                       <  2:3189129/149‑1 (MQ=255)
                                                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                       >  2:3839510/1‑149 (MQ=255)
                                                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                       >  2:3418486/1‑149 (MQ=255)
                                                                                              cGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGc                                                     <  1:3787097/149‑1 (MQ=255)
                                                                                                cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                   <  2:4060523/149‑1 (MQ=255)
                                                                                                cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                   <  1:2133287/149‑1 (MQ=255)
                                                                                                  gCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCggg                                                 >  1:2301547/1‑149 (MQ=255)
                                                                                                   ccATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAACTGCCGGGt                                                >  1:3198927/1‑149 (MQ=255)
                                                                                                      tGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                             >  1:808841/1‑149 (MQ=255)
                                                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                         <  2:1784779/149‑1 (MQ=255)
                                                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                         <  1:383247/149‑1 (MQ=255)
                                                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                         <  2:3198472/149‑1 (MQ=255)
                                                                                                            aCATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCg                                       >  1:4042618/1‑149 (MQ=255)
                                                                                                             cATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGa                                      >  1:4679572/1‑149 (MQ=255)
                                                                                                               tGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATc                                    >  1:1958971/1‑149 (MQ=255)
                                                                                                                 gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAg                                  <  1:3029571/149‑1 (MQ=255)
                                                                                                                  tgtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCCTAAGTGCCGGGTTCGTTGGCGATCAGa                                 >  2:854318/1‑149 (MQ=255)
                                                                                                                     tCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGc                              <  1:58827/149‑1 (MQ=255)
                                                                                                                       ggCGACTGCTTCGCTTTAGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGGACGGTGGCGAGCAGATGCAg                            >  2:1200839/1‑149 (MQ=255)
                                                                                                                           aCTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGa                        >  1:943751/1‑149 (MQ=255)
                                                                                                                            cTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                       <  2:1612768/149‑1 (MQ=255)
                                                                                                                             tGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGt                      <  2:3633931/149‑1 (MQ=255)
                                                                                                                              gCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTc                     <  1:1403208/149‑1 (MQ=255)
                                                                                                                                   gCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt                >  2:3510132/1‑149 (MQ=255)
                                                                                                                                   gCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt                <  1:2923676/149‑1 (MQ=255)
                                                                                                                                    cTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATa               <  2:1560539/149‑1 (MQ=255)
                                                                                                                                    attttGCGACCCACGGGTCGAATGAGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGTCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATa               >  2:2876412/2‑149 (MQ=255)
                                                                                                                                     ttttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAc              >  2:3393520/1‑149 (MQ=255)
                                                                                                                                     ttttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAc              >  2:4716964/1‑149 (MQ=255)
                                                                                                                                      tttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAcc             <  1:1618413/149‑1 (MQ=255)
                                                                                                                                        tGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCgg           <  1:3732605/149‑1 (MQ=255)
                                                                                                                                        tGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCgg           <  1:1344014/149‑1 (MQ=255)
                                                                                                                                           gACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa        >  2:1677033/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCAT  >  CP009273/446223‑446516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑