Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,333 A→T 9.7% intergenic (+88/‑256) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,3330AT 9.7% 682.6 / 11.8 285intergenic (+88/‑256)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base A (92/151);  new base T (19/2);  total (132/153)
Fisher's exact test for biased strand distribution p-value = 2.33e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450187‑450481
                                                                                                                                                   |                                                                                                                                                        
ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAtt                                                                                                                                                        <  1:4230307/149‑1 (MQ=255)
ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAtt                                                                                                                                                        <  2:2685281/149‑1 (MQ=255)
  ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAtttt                                                                                                                                                      <  1:182277/149‑1 (MQ=255)
  ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTGTGTTGTtttt                                                                                                                                                      >  1:3764200/1‑149 (MQ=255)
      aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGgtg‑gtttttgtggtttttttgg                                                                                                                                                  >  1:1224554/1‑145 (MQ=255)
       ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCt                                                                                                                                                 <  1:2194668/149‑1 (MQ=255)
       ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGGATTTTTCGCt                                                                                                                                                 >  2:2160722/1‑149 (MQ=255)
         acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCttt                                                                                                                                               >  2:3379685/1‑149 (MQ=255)
         acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTTCGCttt                                                                                                                                               >  2:4336231/1‑149 (MQ=255)
           acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGGG‑GTTTGTGTGGCTTTTGCGCttttt                                                                                                                                             >  2:4458118/1‑149 (MQ=255)
           acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTGCGCttttt                                                                                                                                             >  2:1905955/1‑149 (MQ=255)
           acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTGAGCttttt                                                                                                                                             >  2:3614457/1‑149 (MQ=255)
           acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTGTCTGGAGTTTGCGCttttt                                                                                                                                             >  2:3614102/1‑149 (MQ=255)
            ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                                            >  2:1098041/1‑149 (MQ=255)
              gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGCCCAGCGGGCCGTGTTG‑TTTTGTCGGGGTTTTGCGCTTTTTGcc                                                                                                                                          >  1:2378452/1‑149 (MQ=255)
               tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGccc                                                                                                                                         <  1:1707307/149‑1 (MQ=255)
               tCTTTGCCTCTCCTCCCTGTCGTGGAGAATTAAGCAGCGCGTAAAAACGCATTTGAAACTGTCGGCTCTTTTCCAGTATGTTTCTAAATATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGccc                                                                                                                                         <  1:4097297/149‑1 (MQ=255)
                 tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGGCTGGAGTTTGCGCTTTTTTccccg                                                                                                                                       >  1:1059186/1‑147 (MQ=255)
                    gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTTCGCTTTTTGCCCAGCAt                                                                                                                                    >  2:1530144/1‑149 (MQ=255)
                    gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTG‑TTTTGTGTTGCTTTTTAGCTTTTTTCCCAGCAt                                                                                                                                    >  1:400322/1‑149 (MQ=255)
                       tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTTGATTTTGCGCTTTTTGCCCCGCATTCa                                                                                                                                 >  2:1051124/1‑149 (MQ=255)
                        ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTGG‑TTTTGTCTGGATTTTGCGCTTTTTTCCCCGCAATCA‑g                                                                                                                               >  1:1118617/1‑149 (MQ=255)
                        ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑g                                                                                                                               <  1:4434916/149‑1 (MQ=255)
                          cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTGTCTGGATTTTGCGCGTTTTTCCCAGCAATCA‑GAc                                                                                                                             >  2:4509878/1‑149 (MQ=255)
                             cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTTTTGGTTTTGGGGTTTTTTGCCACCAGTCaaaaaca                                                                                                                           >  1:4584307/1‑143 (MQ=255)
                              ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTT‑TTTTTTTTGGGTTTTGGGGTTTTTGGCCACCAGTAA‑GAAGaaa                                                                                                                         >  1:1723816/1‑149 (MQ=255)
                                    gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTGTGGATTTTTCGGTTTTTGCCCACCATTAA‑GACGAAAA‑TTGc‑c                                                                                                                 >  1:3901911/1‑149 (MQ=255)
                                    gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGc‑c                                                                                                                 <  1:1067317/149‑1 (MQ=255)
                                      ggAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCg                                                                                                               <  1:466086/149‑1 (MQ=255)
                                       gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGGTTTTTTGGGTTTTTTGCCACCAATCA‑TGCGAAAA‑TTGC‑CCgg                                                                                                              >  1:3353739/1‑149 (MQ=255)
                                         gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGa                                                                                                            <  2:3779978/149‑1 (MQ=255)
                                         gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCgggc                                                                                                            >  2:4195553/1‑148 (MQ=255)
                                            ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGTTTTTTCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAAtt                                                                                                         >  2:2175951/1‑149 (MQ=255)
                                            ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAAtt                                                                                                         <  2:2869336/149‑1 (MQ=255)
                                            ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAAtt                                                                                                         <  1:682576/149‑1 (MQ=255)
                                             gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATtg                                                                                                        <  2:2783620/149‑1 (MQ=255)
                                               aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGTTTTTTGGCTTTTTGGCCAACATTCA‑GACGAAAA‑TTTC‑CCGGGGAttttt                                                                                                      >  2:1843961/1‑146 (MQ=255)
                                               aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGGTTTTTTGGCTTTTTTGACAGCAATAA‑GAACAAAA‑TTTCGCCGGGAAtttg                                                                                                       >  1:3313020/1‑147 (MQ=255)
                                                         aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATAcg                                                                                            <  2:126841/149‑1 (MQ=255)
                                                         aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTTGTGGTTTTTTGGTTTTTTTGCCCCCATAAG‑AAGGAAAA‑ATGC‑CCGGGAGTTGTTaaaaataaa                                                                                             >  1:3593714/1‑145 (MQ=255)
                                                          acaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTGGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATAcgc                                                                                           <  1:3434375/147‑1 (MQ=255)
                                                             aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGAc                                                                                        <  2:1449557/149‑1 (MQ=255)
                                                               gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTGGGGTTTTTGGGCTTTTTGCCCAGCAATAATAACAAAAA‑TTGC‑CGGGGAATT‑TTTAAAAATAAGCGAccg                                                                                      >  1:3146953/1‑147 (MQ=255)
                                                                cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGATTTTTCGCTTTTTTCCCAGCATTCA‑GACGAAAA‑ATGC‑CCGGGACTTGTGAAAAAATACGCGACAgc                                                                                     >  2:1929959/1‑149 (MQ=255)
                                                                cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGTAAAAATTCGCGACAgc                                                                                     >  2:4320119/1‑149 (MQ=255)
                                                                       aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTGTTGTTTTTTGGCTTTTTTGCCAGCCATAA‑GACGAAAA‑TTTC‑CCCGGAATTGTGGAAAAAAAACCGACAgcgccgcaa                                                                              >  1:2655788/1‑144 (MQ=255)
                                                                         cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATaa                                                                           <  2:2443305/149‑1 (MQ=255)
                                                                          tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAAc                                                                          <  1:4287522/149‑1 (MQ=255)
                                                                           gTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAAcc                                                                         <  1:3314373/149‑1 (MQ=255)
                                                                           gTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAAcc                                                                         <  1:718277/149‑1 (MQ=255)
                                                                           gTCGGCGCTTTGCCTGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAAcc                                                                         <  1:4647864/149‑1 (MQ=255)
                                                                              ggCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGtt                                                                      <  2:3908843/149‑1 (MQ=255)
                                                                              ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGtt                                                                      <  2:4494641/149‑1 (MQ=255)
                                                                              ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCA‑TACGCAAA‑TTGC‑CCGGGACTTGTGTAAAAATAAGCGCCCTCGCG‑CAATAACCGtt                                                                      >  2:3724262/1‑149 (MQ=255)
                                                                                ggcTTTTCCTTTATGTTTCTAAAAATTTTTTGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTtct                                                                    <  2:4056026/148‑1 (MQ=255)
                                                                                cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTtct                                                                    <  2:115810/149‑1 (MQ=255)
                                                                                 gcTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTtctc                                                                   <  2:740563/149‑1 (MQ=255)
                                                                                  cTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTGTCTGGATTTTTCGCTTTTTTGCCACCAATAA‑GACCAAAATTTCC‑CCGGGAATT‑TGTAAAAATAAACGACACCGCGCCAAAAACCgaatac                                                                   >  1:654710/1‑144 (MQ=255)
                                                                                   ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTTGGTTTTTCGCTTTTTGCCCAGCATTCA‑TACGAAAA‑TTGC‑CCCGGACATGTTAAAAAAAACGCGACAGCGCG‑CAAAAAACCTTATCGa                                                                 >  1:1196830/1‑149 (MQ=255)
                                                                                   ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGa                                                                 <  2:2282468/149‑1 (MQ=255)
                                                                                      tCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTATGGATTTTTCGCTTTTTTCCCAGCATTAA‑TACGAAAA‑TTTC‑CCCGGAATTTTTAAAAAATACGCGACAGCCCG‑CAATAAACGTTCTCGAATc                                                              >  1:2385854/1‑149 (MQ=255)
                                                                                       ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GAAGAAAA‑TTTC‑CCGGGAATTGTGAAAAAATAAGCGACAGCGCG‑CAATAACCGTTCTAGACTCa                                                             >  2:3951256/1‑149 (MQ=255)
                                                                                         aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTGGATTTTGCGCTTTTTTCCCAGCAATAA‑GAAGAAAA‑ATTC‑CCGGGAAATTTGTAAAAAAAAGCGACAGCGCG‑CAATAAACGTTGTCGACTCATa                                                           >  1:2775866/1‑149 (MQ=255)
                                                                                         aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTATTGATTTTTCGCATTTTTCCCACCCATAA‑GACGAAAA‑ATGC‑CCGGGAAAATTGAAAAAATAAGCGACACCGCG‑CAAAAAACGTTCTAGACACATa                                                           >  2:851987/1‑149 (MQ=255)
                                                                                         aGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATa                                                           <  1:165687/149‑1 (MQ=255)
                                                                                            aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAAAACGCGACAGCGCG‑CAAGAACCGTTCTCGACTCaaaaaa                                                        >  2:621753/1‑149 (MQ=255)
                                                                                            aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑ATGC‑CCGGGAAATGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATaaaa                                                        >  2:621841/1‑149 (MQ=255)
                                                                                             tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAg                                                       <  2:323501/149‑1 (MQ=255)
                                                                                             tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCATTTTGCCCCCCAATAA‑CACGAAAA‑ATGC‑CCCGGCAGTTTTAAAAAATACGCGACAGCCCG‑CAAAAACCCTTCTCGACTCATAAAAg                                                       >  2:1368192/1‑149 (MQ=255)
                                                                                                 gCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATAAGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGAt                                                   >  2:3073531/1‑149 (MQ=255)
                                                                                                   tAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGCCTCATAAAAGTGATGc                                                 >  2:1984439/1‑149 (MQ=255)
                                                                                                     aaaaTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTATGGATTTTTCGCTTTTTGCCCAGCATTAA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGTTTCCg                                               >  2:2592485/4‑149 (MQ=255)
                                                                                                        aTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTGGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                            <  1:1391990/149‑1 (MQ=255)
                                                                                                         ttttATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtat                                           <  2:604170/149‑1 (MQ=255)
                                                                                                         ttttATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtat                                           <  2:527654/149‑1 (MQ=255)
                                                                                                         ttttATGAAAAACGGCCTGCGGGCCGTGGTG‑TTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTAA‑GACGAAAA‑TTGC‑CCGGGCATTGTGAAAAAATAAGCGACAGCGCG‑CAAAAACCGTTCTAGACTCAAAAAAGTGATTCCGCtat                                           >  2:1782835/1‑149 (MQ=255)
                                                                                                            tATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGGTTTTTCGCTTTTTTCCCCGCAATCA‑GACGAAAA‑TTGC‑CCCGGAATTGTGAAAAAATACGCGACAGCGCG‑CAAAAAACCTTCTCGACTCATAAAAGTGTTTGCGCTATAAt                                        >  2:2124535/1‑149 (MQ=255)
                                                                                                                 aaaaCGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAATCA‑GACGAAAA‑ATGC‑CCGGGACTTGTGAAAAAATAAGCGACAGCGCG‑CAAAAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                   >  2:4151179/1‑149 (MQ=255)
                                                                                                                       gCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTa                             <  2:4584307/149‑1 (MQ=255)
                                                                                                                             gggCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTTCCCAGCAATCA‑TACGAAAA‑ATTC‑CCCGGAATTGTGAAAAAATACTCGACAGCGCC‑CAAAAAACCTTATCGACACATAAAAGTGATGCCGCAATAAATCCGCGTACTAATTGaa                       >  2:4307938/1‑149 (MQ=255)
                                                                                                                              ggCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt                      <  1:3282651/149‑1 (MQ=255)
                                                                                                                               gCCTTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATg                     <  1:2175884/149‑1 (MQ=255)
                                                                                                                               gCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATg                     <  1:2484224/149‑1 (MQ=255)
                                                                                                                               gCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATg                     <  2:3490287/149‑1 (MQ=255)
                                                                                                                                ccGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGc                    <  2:702091/149‑1 (MQ=255)
                                                                                                                                 cGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt                   >  2:4707385/1‑149 (MQ=255)
                                                                                                                                  gttttg‑ttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                  <  2:2249397/149‑1 (MQ=255)
                                                                                                                                  gttttg‑ttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                  <  2:4632399/149‑1 (MQ=255)
                                                                                                                                   ttttg‑ttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                 <  2:2222097/149‑1 (MQ=255)
                                                                                                                                    tttg‑ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                <  1:509505/149‑1 (MQ=255)
                                                                                                                                    tttg‑ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                >  1:627335/1‑149 (MQ=255)
                                                                                                                                    tttg‑ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                <  1:2901862/149‑1 (MQ=255)
                                                                                                                                    tttg‑ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                >  1:3680819/1‑149 (MQ=255)
                                                                                                                                    tttg‑ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                <  1:1932141/149‑1 (MQ=255)
                                                                                                                                           ttgtCTGGATTGTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg          <  2:2700105/149‑1 (MQ=255)
                                                                                                                                             gtCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGTAATTTTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat        <  2:3814597/149‑1 (MQ=255)
                                                                                                                                              tCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAGATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       >  1:1881598/1‑149 (MQ=255)
                                                                                                                                              tCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       <  2:1678037/149‑1 (MQ=255)
                                                                                                                                                   aTTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt  >  1:1356574/1‑149 (MQ=255)
                                                                                                                                                   aTTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt  >  1:868421/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                        
GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA‑GACGAAAA‑TTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450187‑450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑