Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 480,820 C→T 100% D86N (GAC→AAC)  acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273480,8200CT100.0% 1037.4 / NA 295D86N (GAC→AAC) acrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (140/155);  total (140/155)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 295 total aligned reads displayed.

ACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGC  >  CP009273/480674‑480965
                                                                                                                                                  |                                                                                                                                                 
aCCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGc                                                                                                                                                 >  1:2090062/1‑149 (MQ=255)
aCCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGc                                                                                                                                                 <  1:1766609/149‑1 (MQ=255)
  cGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTa                                                                                                                                               <  1:1497502/149‑1 (MQ=255)
   gAGCAGTTTCTGATAACGATTAACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCTCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTAc                                                                                                                                              >  1:3179340/1‑149 (MQ=255)
    aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTAcc                                                                                                                                             >  2:2769183/1‑149 (MQ=255)
     gCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACct                                                                                                                                            <  1:1919601/149‑1 (MQ=255)
     gCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACct                                                                                                                                            <  2:3007683/149‑1 (MQ=255)
     gCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACct                                                                                                                                            <  2:1068710/149‑1 (MQ=255)
       aGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttc                                                                                                                                          <  1:2610451/149‑1 (MQ=255)
        gTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttct                                                                                                                                         >  1:1060529/1‑149 (MQ=255)
         tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttctt                                                                                                                                        >  2:432159/1‑149 (MQ=255)
         tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttctt                                                                                                                                        <  1:4500553/149‑1 (MQ=255)
           tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTg                                                                                                                                      <  2:2560233/149‑1 (MQ=255)
              gATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGaaa                                                                                                                                   >  1:1626154/1‑149 (MQ=255)
              gATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGaaa                                                                                                                                   <  2:3345126/149‑1 (MQ=255)
                tAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAAtt                                                                                                                                 >  1:386378/1‑149 (MQ=255)
                tAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAAtt                                                                                                                                 <  1:2800662/149‑1 (MQ=255)
                 aaCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTa                                                                                                                                >  2:2868393/1‑149 (MQ=255)
                  aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                               <  2:1228638/149‑1 (MQ=255)
                  aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                               <  1:3383846/149‑1 (MQ=255)
                  aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                               <  1:4330374/149‑1 (MQ=255)
                  aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                               >  1:3704836/1‑149 (MQ=255)
                   cGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACg                                                                                                                              <  1:2432968/149‑1 (MQ=255)
                     aTTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCt                                                                                                                            >  2:613404/1‑149 (MQ=255)
                       tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTc                                                                                                                          <  1:2253564/149‑1 (MQ=255)
                       tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTc                                                                                                                          >  1:4328137/1‑149 (MQ=255)
                        cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCa                                                                                                                         >  1:4124712/1‑149 (MQ=255)
                         aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAg                                                                                                                        >  1:799109/1‑149 (MQ=255)
                           cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGa                                                                                                                      <  2:1517977/149‑1 (MQ=255)
                           cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGa                                                                                                                      <  1:3320923/149‑1 (MQ=255)
                           cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGa                                                                                                                      <  2:1996529/149‑1 (MQ=255)
                               aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                                                                  <  2:1291741/149‑1 (MQ=255)
                                aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATc                                                                                                                 >  2:3873342/1‑149 (MQ=255)
                                 ttGCGCGATATTGGCTGCAGCCTGGGCTGTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc                                                                                                                <  2:2416349/149‑1 (MQ=255)
                                   gcgcGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCg                                                                                                              >  2:3927217/1‑149 (MQ=255)
                                    cgcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                                                             <  2:3235620/149‑1 (MQ=255)
                                    cgcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                                                             <  1:2186779/149‑1 (MQ=255)
                                    cgcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                                                             <  1:729154/149‑1 (MQ=255)
                                    cgcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                                                             <  2:845928/149‑1 (MQ=255)
                                        atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc                                                                                                         >  2:1763148/1‑149 (MQ=255)
                                          atTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                                                                       <  2:4631915/149‑1 (MQ=255)
                                           ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                                                                      >  1:910317/1‑149 (MQ=255)
                                             ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                                                                    <  1:2949117/149‑1 (MQ=255)
                                             ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                                                                    >  1:196913/1‑149 (MQ=255)
                                               cTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCTCCTGATAGGTCGCAGGATCTATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTAACTTATTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                                                                  <  1:2907128/149‑1 (MQ=255)
                                                tGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGAc                                                                                                 <  1:3637007/149‑1 (MQ=255)
                                                tGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGAc                                                                                                 <  1:3090314/149‑1 (MQ=255)
                                                       tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                                                                          <  1:48206/149‑1 (MQ=255)
                                                         ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                                                        <  2:152591/149‑1 (MQ=255)
                                                          gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGc                                                                                       >  1:4120725/1‑149 (MQ=255)
                                                                gCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                                                 >  1:4417849/1‑149 (MQ=255)
                                                                 ccAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                                <  1:1725196/149‑1 (MQ=255)
                                                                  cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGGTAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                               >  2:1084424/1‑149 (MQ=255)
                                                                  cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                               >  1:2641650/1‑149 (MQ=255)
                                                                   aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                                              <  1:3119923/149‑1 (MQ=255)
                                                                    gATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAg                                                                             >  1:1035923/1‑149 (MQ=255)
                                                                       cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                                          >  1:4210851/1‑149 (MQ=255)
                                                                        aCCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCAc                                                                         >  2:651234/1‑149 (MQ=255)
                                                                         ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                                        >  2:1677337/1‑149 (MQ=255)
                                                                           tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                                                                      >  1:4363304/1‑149 (MQ=255)
                                                                                    gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                                                             <  1:1002547/149‑1 (MQ=255)
                                                                                    gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                                                             >  2:4560831/1‑149 (MQ=255)
                                                                                     tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                            >  2:2169371/1‑149 (MQ=255)
                                                                                         aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                                        <  2:3536391/149‑1 (MQ=255)
                                                                                           gTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                                                      <  2:4155004/149‑1 (MQ=255)
                                                                                              gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGATACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGt                                                   >  1:2350272/1‑149 (MQ=255)
                                                                                              gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGt                                                   <  2:913540/149‑1 (MQ=255)
                                                                                              gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGt                                                   <  1:2692304/149‑1 (MQ=255)
                                                                                                cTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg                                                 >  1:3509938/1‑149 (MQ=255)
                                                                                                 tGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgt                                                >  2:3717009/1‑149 (MQ=255)
                                                                                                       gTCGCATGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                          <  1:1239313/149‑1 (MQ=255)
                                                                                                       gTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                          <  1:3756914/149‑1 (MQ=255)
                                                                                                       gTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                          >  1:3942951/1‑149 (MQ=255)
                                                                                                        tCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGc                                         <  1:4561940/149‑1 (MQ=255)
                                                                                                        tCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGc                                         <  1:1875140/149‑1 (MQ=255)
                                                                                                         cGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCa                                        >  1:402563/1‑149 (MQ=255)
                                                                                                           cAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCaga                                      <  1:2769183/149‑1 (MQ=255)
                                                                                                              gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGt                                   >  2:3450018/1‑149 (MQ=255)
                                                                                                               aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGtt                                  <  1:1664787/149‑1 (MQ=255)
                                                                                                                  aaTCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAg                               <  2:4699404/149‑1 (MQ=255)
                                                                                                                    tCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtt                             <  1:2953962/149‑1 (MQ=255)
                                                                                                                    tCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtt                             >  1:565085/1‑149 (MQ=255)
                                                                                                                         tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc                        >  1:685797/1‑149 (MQ=255)
                                                                                                                         tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc                        >  1:4574865/1‑149 (MQ=255)
                                                                                                                          agagagagACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACt                       >  2:4424258/1‑149 (MQ=255)
                                                                                                                           gagagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTg                      >  2:4042600/1‑149 (MQ=255)
                                                                                                                           gagagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTg                      <  1:2151508/149‑1 (MQ=255)
                                                                                                                           gagagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTGGTGATCTGCAGAGGTTCAGTTTTGACTg                      >  2:4002258/1‑149 (MQ=255)
                                                                                                                             gagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGtt                    >  2:1361692/1‑149 (MQ=255)
                                                                                                                               gagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac                  <  2:4190102/149‑1 (MQ=255)
                                                                                                                               gagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac                  >  1:1410403/1‑149 (MQ=255)
                                                                                                                                 gaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtacta                >  2:3184011/1‑149 (MQ=255)
                                                                                                                                  acacCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtactac               <  1:130742/149‑1 (MQ=255)
                                                                                                                                    acCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGc             >  2:2776195/1‑149 (MQ=255)
                                                                                                                                       tGCTTCGATGGTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCaa          <  2:2985023/149‑1 (MQ=255)
                                                                                                                                         cTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcg        >  1:98599/1‑149 (MQ=255)
                                                                                                                                          ttCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcgg       >  2:4599682/1‑149 (MQ=255)
                                                                                                                                          ttCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcgg       <  2:4368816/149‑1 (MQ=255)
                                                                                                                                              aTGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg   >  2:499097/1‑149 (MQ=255)
                                                                                                                                               tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGc  <  2:1292301/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGC  >  CP009273/480674‑480965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑