Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 481,667 G→A 100% A151T (GCG→ACG)  acrR → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273481,6670GA99.7% 1130.5 / ‑4.7 327A151T (GCG→ACG) acrRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (149/177);  total (149/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 327 total aligned reads displayed.

CAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481521‑481812
                                                                                                                                                  |                                                                                                                                                 
 agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGa                                                                                                                                                <  2:2568565/149‑1 (MQ=255)
 agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGa                                                                                                                                                >  1:4324980/1‑149 (MQ=255)
     gaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAt                                                                                                                                            <  2:4344701/149‑1 (MQ=255)
     gaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAt                                                                                                                                            <  1:2795604/149‑1 (MQ=255)
       aCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATATAACAAACGTTAAAACATTGTATTGAAACGAAAATGt                                                                                                                                          >  1:1854695/1‑149 (MQ=255)
         ggCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                        >  2:979269/1‑149 (MQ=255)
         ggCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                        >  2:3725772/1‑149 (MQ=255)
         ggCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                        >  2:3368223/1‑149 (MQ=255)
           cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGcc                                                                                                                                      <  1:2715116/149‑1 (MQ=255)
            gTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACAATGTATTGAAACGAAAATGTTGCCt                                                                                                                                     >  1:4081742/1‑149 (MQ=255)
             tCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                    >  1:4455667/1‑149 (MQ=255)
             tCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                    >  1:2647171/1‑149 (MQ=255)
              cGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGc                                                                                                                                   <  1:1159897/149‑1 (MQ=255)
              cGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGc                                                                                                                                   >  2:1272103/1‑149 (MQ=255)
                attattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCgg                                                                                                                                 <  1:3676011/149‑1 (MQ=255)
                 ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGa                                                                                                                                >  2:1126074/1‑149 (MQ=255)
                 ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGa                                                                                                                                <  1:1571003/149‑1 (MQ=255)
                    ttGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAttt                                                                                                                             <  2:4451368/149‑1 (MQ=255)
                    ttGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAttt                                                                                                                             <  1:1976216/149‑1 (MQ=255)
                     tGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCTTATAGAACAAACGTTAAAACATTTTATTGAAACGAAAATGTTGCCTGCGGATTTa                                                                                                                            >  1:4736633/1‑149 (MQ=255)
                      gATGGAGATTATATGCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTaa                                                                                                                           <  1:1716601/149‑1 (MQ=255)
                        tGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATg                                                                                                                         >  2:2305494/1‑149 (MQ=255)
                         ggAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGa                                                                                                                        <  2:1510094/149‑1 (MQ=255)
                          gagaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAc                                                                                                                       <  1:2037181/149‑1 (MQ=255)
                               tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTc                                                                                                                  <  1:3534977/149‑1 (MQ=255)
                                atatTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcg                                                                                                                 >  2:1577724/1‑149 (MQ=255)
                                            tGCGAAGTTGTCGGAGAAAGGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     <  2:4100116/149‑1 (MQ=255)
                                               gAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACTCGTCGCGCAGCAATtattat                                                                                                  >  1:2376843/1‑149 (MQ=255)
                                                aaTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATg                                                                                                 >  2:2954648/1‑149 (MQ=255)
                                                 aTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgc                                                                                                >  1:3964292/1‑149 (MQ=255)
                                                   ttGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgcgc                                                                                              >  1:3059609/1‑149 (MQ=255)
                                                     gTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                            >  1:1253654/1‑149 (MQ=255)
                                                       cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                          <  1:2641860/149‑1 (MQ=255)
                                                         gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        <  2:689827/149‑1 (MQ=255)
                                                         gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  1:1768262/1‑149 (MQ=255)
                                                         gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  1:2482750/1‑149 (MQ=255)
                                                          agaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                       >  2:2748090/1‑149 (MQ=255)
                                                          agaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                       <  1:3719034/149‑1 (MQ=255)
                                                           gaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTATTGACGCGTCGCGCAGCAATTATTATGCGCGGCTatat                                                                                      >  2:460412/1‑149 (MQ=255)
                                                           gaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTatat                                                                                      <  2:3423594/149‑1 (MQ=255)
                                                           gaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTatat                                                                                      <  1:4562779/149‑1 (MQ=255)
                                                               tGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                  >  2:3009516/1‑149 (MQ=255)
                                                               tGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                  >  1:1410868/1‑149 (MQ=255)
                                                               tGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                  <  2:3564794/149‑1 (MQ=255)
                                                                   tgttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCACTTATTATGCGCGGCTATATTTCCGGcc                                                                              >  2:1056036/1‑149 (MQ=255)
                                                                    gttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCt                                                                             <  1:3119629/149‑1 (MQ=255)
                                                                     ttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTg                                                                            >  2:720401/1‑149 (MQ=255)
                                                                          cAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGa                                                                       <  1:4537764/149‑1 (MQ=255)
                                                                            aCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaa                                                                     >  1:170246/1‑149 (MQ=255)
                                                                              aGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAAc                                                                   <  1:2268311/149‑1 (MQ=255)
                                                                               ggCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACt                                                                  <  1:1289851/149‑1 (MQ=255)
                                                                                 cacaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTgg                                                                <  2:3112979/149‑1 (MQ=255)
                                                                                 cacaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTgg                                                                <  2:1197073/149‑1 (MQ=255)
                                                                                 cacaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTgg                                                                <  2:3585347/149‑1 (MQ=255)
                                                                                  acaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGc                                                               >  1:3199095/1‑149 (MQ=255)
                                                                                    aaCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctc                                                             >  1:294109/1‑149 (MQ=255)
                                                                                     aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctct                                                            <  1:3873397/149‑1 (MQ=255)
                                                                                      cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                           >  2:3055892/1‑149 (MQ=255)
                                                                                       gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                          <  2:2400330/149‑1 (MQ=255)
                                                                                       gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                          >  1:4202451/1‑149 (MQ=255)
                                                                                        tAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                         >  1:2831795/1‑149 (MQ=255)
                                                                                           tctcTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGccc                                                      <  1:745760/149‑1 (MQ=255)
                                                                                              ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                   <  1:2599058/149‑1 (MQ=255)
                                                                                              ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                   <  1:3242930/149‑1 (MQ=255)
                                                                                              ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                   >  2:1906289/1‑149 (MQ=255)
                                                                                                gtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                 >  1:2018987/1‑149 (MQ=255)
                                                                                                 tctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACCTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAAt                                                >  2:4417764/1‑149 (MQ=255)
                                                                                                   tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  1:1312545/149‑1 (MQ=255)
                                                                                                    ggAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                             <  2:2032276/149‑1 (MQ=255)
                                                                                                    ggAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                             <  2:618552/149‑1 (MQ=255)
                                                                                                     gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                            >  2:3252515/1‑149 (MQ=255)
                                                                                                     gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                            <  2:3994533/149‑1 (MQ=255)
                                                                                                     gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                            <  1:1006261/149‑1 (MQ=255)
                                                                                                      aaaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtttt                                           >  1:1499979/1‑149 (MQ=255)
                                                                                                      aaaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtttt                                           <  1:4015873/149‑1 (MQ=255)
                                                                                                        aGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGa                                         >  2:1723037/1‑149 (MQ=255)
                                                                                                         gTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGAt                                        <  2:2218537/149‑1 (MQ=255)
                                                                                                          ttATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATc                                       >  2:529901/1‑149 (MQ=255)
                                                                                                            aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     <  1:2123009/149‑1 (MQ=255)
                                                                                                            aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     <  1:2123343/149‑1 (MQ=255)
                                                                                                                ccGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaa                                 >  1:2510226/1‑149 (MQ=255)
                                                                                                                   tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaag                              >  2:1469072/1‑149 (MQ=255)
                                                                                                                       gAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCACTTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                          >  2:2262702/1‑149 (MQ=255)
                                                                                                                       gAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                          >  2:209921/1‑149 (MQ=255)
                                                                                                                       gAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                          >  1:4041086/1‑149 (MQ=255)
                                                                                                                           aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      >  2:811942/1‑149 (MQ=255)
                                                                                                                           aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      <  2:1776164/149‑1 (MQ=255)
                                                                                                                              cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAt                   <  1:2921083/149‑1 (MQ=255)
                                                                                                                              cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAt                   <  2:4247972/149‑1 (MQ=255)
                                                                                                                              cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAt                   <  2:4496942/149‑1 (MQ=255)
                                                                                                                               gTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  >  2:4218111/1‑149 (MQ=255)
                                                                                                                               gTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGATTTATTTCCGGCCTGATGGAAAACTGGCTATTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  <  2:3927050/149‑1 (MQ=255)
                                                                                                                                 tAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                >  1:597001/1‑149 (MQ=255)
                                                                                                                                 tAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                <  1:3512014/149‑1 (MQ=255)
                                                                                                                                 tAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                <  2:3860/149‑1 (MQ=255)
                                                                                                                                     aCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTg            <  2:2647171/149‑1 (MQ=255)
                                                                                                                                          gTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATc       <  2:4081528/149‑1 (MQ=255)
                                                                                                                                            aTTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCtt     >  1:643817/1‑149 (MQ=255)
                                                                                                                                               gAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACt  >  2:4756457/1‑149 (MQ=255)
                                                                                                                                               gAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACt  <  1:1936334/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481521‑481812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑