Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 509,737 G→A 100% E97K (GAG→AAG)  cueR → copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273509,7370GA98.7% 819.9 / ‑0.1 240E97K (GAG→AAG) cueRcopper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (110/126);  total (112/128)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTA  >  CP009273/509591‑509882
                                                                                                                                                  |                                                                                                                                                 
 cTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                                >  1:3771314/1‑149 (MQ=255)
    aCTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGc                                                                                                                                             >  2:4610487/1‑149 (MQ=255)
          ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCa                                                                                                                                       <  1:226368/149‑1 (MQ=255)
           cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAt                                                                                                                                      >  1:1031298/1‑149 (MQ=255)
           cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAt                                                                                                                                      <  1:3555755/149‑1 (MQ=255)
             ggCACGGCAGGTGGGATTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                    <  1:2529749/149‑1 (MQ=255)
                  ggCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAc                                                                                                                               >  2:3823088/1‑149 (MQ=255)
                   gCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAcc                                                                                                                              <  2:2178009/149‑1 (MQ=255)
                      ggTGGGCTTTAACCTGGAAGAGCGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                           <  1:4038186/149‑1 (MQ=255)
                      ggTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                           >  2:3968443/1‑149 (MQ=255)
                      ggTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                           <  2:2473799/149‑1 (MQ=255)
                      ggTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                           >  2:3679573/1‑149 (MQ=255)
                      ggTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                           <  1:3495790/149‑1 (MQ=255)
                       gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgct                                                                                                                          >  1:2093841/1‑149 (MQ=255)
                       gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgct                                                                                                                          <  1:547379/149‑1 (MQ=255)
                        tGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctg                                                                                                                         >  1:70313/1‑149 (MQ=255)
                         gggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                        >  1:875415/1‑149 (MQ=255)
                         gggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                        >  2:4231479/1‑149 (MQ=255)
                            cTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTgg                                                                                                                     >  2:1470134/1‑149 (MQ=255)
                               tAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCAc                                                                                                                  >  1:1665888/1‑149 (MQ=255)
                                aaCCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATGGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCAca                                                                                                                 >  1:4214017/1‑148 (MQ=255)
                                   cTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGc                                                                                                              >  1:2068853/1‑149 (MQ=255)
                                    tGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCg                                                                                                             <  1:4454323/149‑1 (MQ=255)
                                       aaGAGAGCGGCGAGCTGGTGAATCTGTTTCACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAt                                                                                                          <  2:2625760/149‑1 (MQ=255)
                                        agagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtg                                                                                                         >  2:1219776/1‑149 (MQ=255)
                                          agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcc                                                                                                       <  2:1161174/149‑1 (MQ=255)
                                             gcggcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgc                                                                                                    >  2:3234217/1‑149 (MQ=255)
                                                gcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCt                                                                                                 <  1:2623884/149‑1 (MQ=255)
                                                    gCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCg                                                                                             >  2:10943/1‑149 (MQ=255)
                                                    gCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCg                                                                                             >  2:2823521/1‑149 (MQ=255)
                                                    gCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCg                                                                                             >  2:2404508/1‑149 (MQ=255)
                                                       ggTGAATCTGTTTACCGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATg                                                                                          <  2:842064/149‑1 (MQ=255)
                                                       ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATg                                                                                          <  1:3450475/149‑1 (MQ=255)
                                                         tGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAc                                                                                        <  2:4467044/149‑1 (MQ=255)
                                                         tGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAc                                                                                        <  2:3716118/149‑1 (MQ=255)
                                                         tGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAc                                                                                        >  1:3329933/1‑149 (MQ=255)
                                                          gAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                       <  2:4378920/149‑1 (MQ=255)
                                                                gTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCg                                                                                 >  1:1438596/1‑149 (MQ=255)
                                                                gTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCg                                                                                 >  1:1438916/1‑149 (MQ=255)
                                                                gTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCg                                                                                 <  2:2997925/149‑1 (MQ=255)
                                                                 tttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGTAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa                                                                                >  2:3474131/1‑149 (MQ=255)
                                                                         cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGa                                                                        <  1:4135616/149‑1 (MQ=255)
                                                                         cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGa                                                                        >  1:369521/1‑149 (MQ=255)
                                                                         cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGa                                                                        >  2:2876966/1‑149 (MQ=255)
                                                                          ccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt                                                                       <  2:2332143/149‑1 (MQ=255)
                                                                          ccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt                                                                       <  2:3517677/149‑1 (MQ=255)
                                                                            gCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                     >  1:4414021/1‑149 (MQ=255)
                                                                            gCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                     <  1:1577890/149‑1 (MQ=255)
                                                                            gCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                     >  1:3952596/1‑149 (MQ=255)
                                                                             cAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTAt                                                                    <  1:2398028/149‑1 (MQ=255)
                                                                              aGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGCATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGCTtctc                                                                   >  1:3357173/1‑149 (MQ=255)
                                                                              aGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATc                                                                   >  1:3356376/1‑149 (MQ=255)
                                                                              aGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATc                                                                   >  1:675454/1‑149 (MQ=255)
                                                                                  gaaaatcgcCTACGTCAAACTGCGCACGATGGAGAAGGTGGAGGAGATATAAAGACACATTGAGAAGCTGCAATCCAAGCGCGACCAGCTGCTGGCACTGGCGAATGCCAGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaaa                                                               >  2:3991885/7‑149 (MQ=255)
                                                                                  gCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaaa                                                               <  2:709087/149‑1 (MQ=255)
                                                                                     caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtc                                                            <  1:2952302/149‑1 (MQ=255)
                                                                                      aGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtct                                                           <  2:2193072/149‑1 (MQ=255)
                                                                                       gcgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctc                                                          <  2:790348/149‑1 (MQ=255)
                                                                                         gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctctc                                                        <  2:4182637/149‑1 (MQ=255)
                                                                                         gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctctc                                                        <  2:4516433/149‑1 (MQ=255)
                                                                                         gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctctc                                                        <  2:612752/149‑1 (MQ=255)
                                                                                          ccGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTcc                                                       <  2:3355784/149‑1 (MQ=255)
                                                                                          ccGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTcc                                                       <  2:3195340/149‑1 (MQ=255)
                                                                                          ccGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTcc                                                       <  2:1002468/149‑1 (MQ=255)
                                                                                             aCGTCAAACGGCTCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgc                                                    <  1:1340278/149‑1 (MQ=255)
                                                                                                tCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgc                                                 <  1:615459/149‑1 (MQ=255)
                                                                                                  aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGctgctg                                               <  1:3553624/149‑1 (MQ=255)
                                                                                                      ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtcat                                           >  1:4247656/1‑149 (MQ=255)
                                                                                                      ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtcat                                           <  2:875415/149‑1 (MQ=255)
                                                                                                       gcgcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGTGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtcatc                                          <  2:2227272/149‑1 (MQ=255)
                                                                                                         gcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTcatcat                                        <  2:137753/149‑1 (MQ=255)
                                                                                                         gcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTcatcat                                        >  2:4051579/1‑149 (MQ=255)
                                                                                                          cACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCatcatc                                       <  1:4673155/149‑1 (MQ=255)
                                                                                                           aCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCg                                      <  1:1917383/149‑1 (MQ=255)
                                                                                                           aCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCg                                      >  1:51051/1‑149 (MQ=255)
                                                                                                                ggAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAg                                 >  1:4762369/1‑149 (MQ=255)
                                                                                                                  agaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAggg                               <  1:366847/149‑1 (MQ=255)
                                                                                                                   gaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGt                              <  2:3967213/149‑1 (MQ=255)
                                                                                                                    aaGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGTGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                             >  2:1416197/1‑149 (MQ=255)
                                                                                                                    aaGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                             >  2:1871661/1‑149 (MQ=255)
                                                                                                                      ggTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAt                           <  1:1248971/149‑1 (MQ=255)
                                                                                                                       gTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAtt                          <  2:2082673/149‑1 (MQ=255)
                                                                                                                         ggCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTaa                        <  1:1470134/149‑1 (MQ=255)
                                                                                                                          gCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAag                       >  2:4324150/1‑149 (MQ=255)
                                                                                                                           cGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAaga                      <  2:1188030/149‑1 (MQ=255)
                                                                                                                           cGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAaga                      <  2:2110997/149‑1 (MQ=255)
                                                                                                                             gagaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                    <  1:2205003/149‑1 (MQ=255)
                                                                                                                             gagaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                    >  1:3783187/1‑149 (MQ=255)
                                                                                                                             gagaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                    >  2:1436846/1‑149 (MQ=255)
                                                                                                                               gaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag                  >  2:4327067/1‑149 (MQ=255)
                                                                                                                                aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga                 >  1:2862969/1‑149 (MQ=255)
                                                                                                                                    aaCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc             >  2:1725735/1‑149 (MQ=255)
                                                                                                                                    aaCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc             <  2:2797529/149‑1 (MQ=255)
                                                                                                                                     aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt            >  2:3047583/1‑149 (MQ=255)
                                                                                                                                     aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt            >  1:4727138/1‑149 (MQ=255)
                                                                                                                                     aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt            >  2:3906333/1‑149 (MQ=255)
                                                                                                                                      cGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTc           <  1:3139528/149‑1 (MQ=255)
                                                                                                                                       gACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg          >  2:2496520/1‑149 (MQ=255)
                                                                                                                                               gagaagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTa  >  2:2552331/1‑149 (MQ=255)
                                                                                                                                               gagaagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTa  <  1:1763937/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTA  >  CP009273/509591‑509882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑