Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,496 T→G 83.0% pseudogene (401/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,4960TG83.0% 791.9 / 104.4 274pseudogene (401/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base T (17/30);  new base G (109/118);  total (126/148)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 278 total aligned reads displayed.

TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA  >  CP009273/524350‑524641
                                                                                                                                                  |                                                                                                                                                  
tCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGgggt                                                                                                                                                  <  2:1486779/149‑1 (MQ=255)
    tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAc                                                                                                                                              >  2:3510816/1‑149 (MQ=255)
     aaCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                             <  2:262179/149‑1 (MQ=255)
     aaCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                             <  2:3135569/149‑1 (MQ=255)
        aaaTGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTACGATACCAGGGCAAGGGGATGGGGTAAACC‑ttt                                                                                                                                         >  2:4673572/1‑149 (MQ=255)
        aaaTGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑ttt                                                                                                                                         <  1:3504520/149‑1 (MQ=255)
         aaTGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑tttt                                                                                                                                        <  1:2030918/149‑1 (MQ=255)
          atgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑ttttt                                                                                                                                       <  1:2777287/149‑1 (MQ=255)
          atgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑ttttt                                                                                                                                       <  2:1941733/149‑1 (MQ=255)
           tgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑tttttt                                                                                                                                      <  1:120598/149‑1 (MQ=255)
             atgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑tttttttt                                                                                                                                    <  1:16100/149‑1 (MQ=255)
                 tgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTttgttg                                                                                                                                <  1:2268965/149‑1 (MQ=255)
                   ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGt                                                                                                                              <  2:3339295/149‑1 (MQ=255)
                   ggggGAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCATATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGt                                                                                                                              <  2:2995367/149‑1 (MQ=255)
                    gggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTg                                                                                                                             >  1:1598331/1‑149 (MQ=255)
                    gggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTg                                                                                                                             >  1:1246784/1‑149 (MQ=255)
                    gggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTg                                                                                                                             >  1:1246805/1‑149 (MQ=255)
                     ggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGa                                                                                                                            <  1:3571017/149‑1 (MQ=255)
                         ggTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAAtt                                                                                                                        <  2:1794903/149‑1 (MQ=255)
                           tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTaa                                                                                                                      <  1:2177585/149‑1 (MQ=255)
                            ttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTaa                                                                                                                      <  1:3345016/149‑1 (MQ=255)
                              aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCTAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGt                                                                                                                   <  1:212372/149‑1 (MQ=255)
                              aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGt                                                                                                                   <  1:399183/149‑1 (MQ=255)
                               aGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                  <  2:3255679/149‑1 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTa                                                                                                                 >  2:926042/1‑149 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTa                                                                                                                 <  2:3880331/149‑1 (MQ=255)
                                 aaaaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTAc                                                                                                                <  2:4165495/149‑1 (MQ=255)
                                 aaaaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTAc                                                                                                                <  2:1257534/149‑1 (MQ=255)
                                    aCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCa                                                                                                             <  2:2350495/149‑1 (MQ=255)
                                    aCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCa                                                                                                             <  1:759180/149‑1 (MQ=255)
                                     cATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCat                                                                                                            <  2:1079205/149‑1 (MQ=255)
                                         gACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCattatt                                                                                                        <  1:2304781/149‑1 (MQ=255)
                                         gACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCattatt                                                                                                        <  1:3189467/149‑1 (MQ=255)
                                         gACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCattatt                                                                                                        <  1:1936867/149‑1 (MQ=255)
                                            aGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTta                                                                                                     >  2:4366950/1‑149 (MQ=255)
                                             gTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTtat                                                                                                    >  1:2245328/1‑149 (MQ=255)
                                               gAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATaa                                                                                                  <  1:682244/149‑1 (MQ=255)
                                                 gggCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACa                                                                                                >  1:1075340/1‑149 (MQ=255)
                                                  ggCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACaa                                                                                               <  2:1158592/149‑1 (MQ=255)
                                                      aGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                           >  2:1585488/1‑149 (MQ=255)
                                                      aGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                           <  2:1393801/149‑1 (MQ=255)
                                                       gggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                          <  2:663866/149‑1 (MQ=255)
                                                       gggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                          >  2:2007923/1‑149 (MQ=255)
                                                           aCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAg                                                                                      <  1:865123/149‑1 (MQ=255)
                                                            cAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGc                                                                                     <  1:1019980/149‑1 (MQ=255)
                                                             aaGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCt                                                                                    <  1:4303546/149‑1 (MQ=255)
                                                             aaGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCt                                                                                    <  1:1150674/149‑1 (MQ=255)
                                                              aGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTg                                                                                   >  2:1900701/1‑149 (MQ=255)
                                                              aGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑‑TTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTgg                                                                                  <  2:3210442/149‑1 (MQ=255)
                                                               gTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑‑TTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                                 >  2:3046278/1‑149 (MQ=255)
                                                                tGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                                 <  2:4767832/149‑1 (MQ=255)
                                                                 ggCGAAAATCGTATCAAAGAATGATCCGGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑‑TTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGTAtt                                                                               >  1:4233084/1‑149 (MQ=255)
                                                                 ggCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                                <  2:375070/149‑1 (MQ=255)
                                                                  gCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAtt                                                                               >  2:1861375/1‑149 (MQ=255)
                                                                    gAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                             <  1:3663658/149‑1 (MQ=255)
                                                                      aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCt                                                                           <  2:4430691/149‑1 (MQ=255)
                                                                       aaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                          >  1:3783813/1‑149 (MQ=255)
                                                                       aaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                          >  2:126344/1‑149 (MQ=255)
                                                                            tATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCt                                                                     >  1:809759/1‑149 (MQ=255)
                                                                             aTCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCtt                                                                    <  2:3404072/149‑1 (MQ=255)
                                                                             aTCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCtt                                                                    >  2:358137/1‑149 (MQ=255)
                                                                                  aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                               <  2:2219184/149‑1 (MQ=255)
                                                                                  aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                               >  1:448952/1‑149 (MQ=255)
                                                                                        aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTaa                                                         <  1:4142315/149‑1 (MQ=255)
                                                                                        aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTaa                                                         <  1:1731871/149‑1 (MQ=255)
                                                                                         tCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑‑TTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAtt                                                       >  2:4526474/1‑149 (MQ=255)
                                                                                         tCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAGACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                        <  1:2879126/149‑1 (MQ=255)
                                                                                            aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGc                                                     <  1:3393741/149‑1 (MQ=255)
                                                                                             gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATTTTACGCATTATTTTATAAAAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                    >  1:495529/1‑149 (MQ=255)
                                                                                             gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                    >  1:3330665/1‑149 (MQ=255)
                                                                                             gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                    >  1:3279667/1‑149 (MQ=255)
                                                                                               tACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTg                                                  <  2:2866211/149‑1 (MQ=255)
                                                                                                      aaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                           <  1:255097/149‑1 (MQ=255)
                                                                                                        aGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAagag                                         <  1:2412910/149‑1 (MQ=255)
                                                                                                          tgtgGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTc                                       <  1:2396848/149‑1 (MQ=255)
                                                                                                           gtggttTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAAC‑TTTTTTTTGTTTGTTAAATTAATTTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCt                                      <  1:4270977/149‑1 (MQ=255)
                                                                                                           gtggtgTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCt                                      <  2:4644558/149‑1 (MQ=255)
                                                                                                               tgtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                  >  2:441820/1‑149 (MQ=255)
                                                                                                               tgtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGAAGAGTAATTGATTGAATTAAGAGTCTATca                                  >  2:2531095/1‑148 (MQ=255)
                                                                                                                   aaTATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                              >  2:2709421/1‑149 (MQ=255)
                                                                                                                     tatGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATc                            >  2:3081293/1‑149 (MQ=255)
                                                                                                                     tatGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATc                            >  1:4034358/1‑149 (MQ=255)
                                                                                                                       tGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGa                          >  2:4742000/1‑149 (MQ=255)
                                                                                                                       tGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGa                          >  1:857990/1‑149 (MQ=255)
                                                                                                                          tAAGATACGAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                       <  2:408882/149‑1 (MQ=255)
                                                                                                                          tAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                       >  1:650645/1‑149 (MQ=255)
                                                                                                                            aGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcg                     >  1:2817560/1‑149 (MQ=255)
                                                                                                                             gATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgt                    >  1:916516/1‑149 (MQ=255)
                                                                                                                             gATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgt                    >  1:1332178/1‑149 (MQ=255)
                                                                                                                             gATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgt                    <  1:4714498/149‑1 (MQ=255)
                                                                                                                                aCCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCaa                 <  1:3578646/149‑1 (MQ=255)
                                                                                                                                    gggCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc             >  1:465286/1‑149 (MQ=255)
                                                                                                                                    gggCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc             <  1:4044370/149‑1 (MQ=255)
                                                                                                                                    gggCAAGGGGATGGGGTAAACC‑TGTTTTTTGTTGGTGAAATTAATGGTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCGGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc             <  2:532751/149‑1 (MQ=255)
                                                                                                                                        aaGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg         <  1:4367863/149‑1 (MQ=255)
                                                                                                                                         aGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGt        >  1:164476/1‑149 (MQ=255)
                                                                                                                                         aGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGt        >  2:628499/1‑149 (MQ=255)
                                                                                                                                             gATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAgg    >  2:3998320/1‑149 (MQ=255)
                                                                                                                                             gATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAgg    >  2:3692805/1‑149 (MQ=255)
                                                                                                                                               tGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGaa  <  1:2614069/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA  >  CP009273/524350‑524641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑