Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 546,491 G→T 100% V199V (GTG→GTT)  ybcF → putative carbonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273546,4910GT99.2% 881.4 / ‑2.0 263V199V (GTG→GTT) ybcFputative carbonate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (132/129);  total (133/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

TTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAG  >  CP009273/546345‑546636
                                                                                                                                                  |                                                                                                                                                 
ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttat                                                                                                                                                 <  2:4498161/149‑1 (MQ=255)
 tGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                <  1:3609221/149‑1 (MQ=255)
 tGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                <  1:251926/149‑1 (MQ=255)
 tGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                <  2:1908989/149‑1 (MQ=255)
  gcgcCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTAGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttattg                                                                                                                                               >  2:1080419/1‑149 (MQ=255)
     ccGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATa                                                                                                                                            <  1:2476797/149‑1 (MQ=255)
          tggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAt                                                                                                                                       <  1:2898080/149‑1 (MQ=255)
          tggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCAGGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGag                                                                                                                                       >  1:1001463/1‑148 (MQ=255)
           ggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtc                                                                                                                                      <  1:2561175/149‑1 (MQ=255)
               gCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                  <  2:2471206/149‑1 (MQ=255)
               gCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                  <  2:223177/149‑1 (MQ=255)
               gCGTCTCCGCAACCGCGTAAAAGTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                  <  2:4021694/149‑1 (MQ=255)
                 gTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccg                                                                                                                                >  1:2220338/1‑149 (MQ=255)
                  tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccgc                                                                                                                               >  1:4187497/1‑149 (MQ=255)
                  tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccgc                                                                                                                               >  1:2288943/1‑149 (MQ=255)
                  tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccgc                                                                                                                               <  1:2786199/149‑1 (MQ=255)
                         aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTg                                                                                                                        >  1:3546778/1‑149 (MQ=255)
                          aCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                       >  2:2259575/1‑149 (MQ=255)
                              cgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGc                                                                                                                   <  2:1485788/149‑1 (MQ=255)
                                    aTTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCa                                                                                                             >  2:2625411/1‑149 (MQ=255)
                                      tctcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGa                                                                                                           <  1:3817869/149‑1 (MQ=255)
                                       ctcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGAt                                                                                                          <  2:204087/149‑1 (MQ=255)
                                       ctcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGAt                                                                                                          <  1:1717944/149‑1 (MQ=255)
                                         cGACAGCGAAGCCATCGAGATGTTGCAAAAAGAGTGGCATGTTGTGATTTGCAGTGGCGGAGGCGGTGTGCCTGTGACGTATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGAGTTGCTCGCCGAGCAGATTa                                                                                                        >  1:2749122/1‑149 (MQ=255)
                                           aCAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAt                                                                                                      >  1:1101496/1‑149 (MQ=255)
                                            cAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATg                                                                                                     >  1:843907/1‑149 (MQ=255)
                                             aGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGc                                                                                                    <  2:2642979/149‑1 (MQ=255)
                                             aGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGc                                                                                                    <  2:2309997/149‑1 (MQ=255)
                                              gCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCa                                                                                                   >  1:4085509/1‑149 (MQ=255)
                                              gCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCa                                                                                                   >  1:4471104/1‑149 (MQ=255)
                                                 aaGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAt                                                                                                >  2:4259604/1‑149 (MQ=255)
                                                    ccATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGa                                                                                             >  2:1666580/1‑149 (MQ=255)
                                                     cATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                            <  2:3094374/149‑1 (MQ=255)
                                                     cATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                            >  1:1792446/1‑149 (MQ=255)
                                                      aTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACt                                                                                           <  2:1223634/149‑1 (MQ=255)
                                                      aTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACt                                                                                           >  2:1713129/1‑149 (MQ=255)
                                                      aTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACt                                                                                           <  2:1223966/149‑1 (MQ=255)
                                                       tCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTg                                                                                          >  1:138974/1‑149 (MQ=255)
                                                         gAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                        >  2:3926235/1‑149 (MQ=255)
                                                              gttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATcc                                                                                   >  1:1725246/1‑149 (MQ=255)
                                                                  cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAc                                                                               <  1:901488/149‑1 (MQ=255)
                                                                  cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAc                                                                               >  1:1464065/1‑149 (MQ=255)
                                                                  cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAc                                                                               >  1:1521174/1‑149 (MQ=255)
                                                                  cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAc                                                                               <  1:557014/149‑1 (MQ=255)
                                                                    cAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCg                                                                             >  1:3567242/1‑149 (MQ=255)
                                                                     aaaGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGa                                                                            >  2:4397051/1‑149 (MQ=255)
                                                                          ggggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg                                                                       <  1:1431981/149‑1 (MQ=255)
                                                                          ggggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg                                                                       >  1:3001919/1‑149 (MQ=255)
                                                                           gggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGa                                                                      <  1:1704299/149‑1 (MQ=255)
                                                                            ggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGAt                                                                     >  1:1348892/1‑149 (MQ=255)
                                                                              cATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGc                                                                   <  1:4390741/149‑1 (MQ=255)
                                                                              cATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGc                                                                   <  1:535645/149‑1 (MQ=255)
                                                                               aTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTTATCCTCACCGATGCTGATGCg                                                                  >  2:4125917/1‑149 (MQ=255)
                                                                               aTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCg                                                                  >  2:4637452/1‑149 (MQ=255)
                                                                                tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCgg                                                                 >  2:1822123/1‑149 (MQ=255)
                                                                                tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAATTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCgg                                                                 >  1:3363375/1‑149 (MQ=255)
                                                                                 gtggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGGTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGt                                                                <  2:2959788/149‑1 (MQ=255)
                                                                                  tggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGta                                                               >  2:1194260/1‑149 (MQ=255)
                                                                                   ggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                              >  1:1567197/1‑149 (MQ=255)
                                                                                   ggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                              <  1:4641831/149‑1 (MQ=255)
                                                                                    gtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtata                                                             >  2:872435/1‑149 (MQ=255)
                                                                                    gtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtata                                                             <  1:675862/149‑1 (MQ=255)
                                                                                      gATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATg                                                           <  1:740393/149‑1 (MQ=255)
                                                                                       aTTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGACCCTCACCGATGCTGATGCGGTATATGa                                                          <  1:3952371/149‑1 (MQ=255)
                                                                                        tttGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaa                                                         <  1:468293/149‑1 (MQ=255)
                                                                                        tttGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaa                                                         >  2:678501/1‑149 (MQ=255)
                                                                                        tttGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaa                                                         >  2:1024876/1‑149 (MQ=255)
                                                                                        tttGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaa                                                         >  2:584500/1‑149 (MQ=255)
                                                                                           gCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                      >  1:1006603/1‑149 (MQ=255)
                                                                                           gCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                      >  2:3598864/1‑149 (MQ=255)
                                                                                           gCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                      <  1:3432588/149‑1 (MQ=255)
                                                                                             aGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTg                                                    <  1:1593377/149‑1 (MQ=255)
                                                                                              gTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg                                                   <  2:522591/149‑1 (MQ=255)
                                                                                                ggcggcggcGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                 <  1:370532/149‑1 (MQ=255)
                                                                                                ggcggcggcGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                 <  2:3775160/149‑1 (MQ=255)
                                                                                                  cggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGaa                                               <  1:2704480/149‑1 (MQ=255)
                                                                                                      ggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgcc                                           >  2:1986415/1‑149 (MQ=255)
                                                                                                      ggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgcc                                           <  1:1764759/149‑1 (MQ=255)
                                                                                                       gcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg                                          >  1:238703/1‑149 (MQ=255)
                                                                                                           tgtgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcagc                                      >  2:3027322/1‑149 (MQ=255)
                                                                                                                cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGt                                 <  2:2424724/149‑1 (MQ=255)
                                                                                                                   tgacggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGcc                              >  1:4132105/1‑149 (MQ=255)
                                                                                                                   tgacggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGcc                              <  2:3875916/149‑1 (MQ=255)
                                                                                                                      cggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCAtt                           <  1:249925/149‑1 (MQ=255)
                                                                                                                       ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTc                          >  1:3059338/1‑149 (MQ=255)
                                                                                                                       ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTc                          <  2:1751160/149‑1 (MQ=255)
                                                                                                                           gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCa                      <  1:2358123/149‑1 (MQ=255)
                                                                                                                              ggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTACTGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGc                   <  1:3252499/149‑1 (MQ=255)
                                                                                                                              ggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGc                   >  2:4297692/1‑149 (MQ=255)
                                                                                                                              ggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGc                   <  2:433229/149‑1 (MQ=255)
                                                                                                                               gaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGcc                  >  2:618502/1‑149 (MQ=255)
                                                                                                                                    gggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACAcc             >  2:3149109/1‑149 (MQ=255)
                                                                                                                                    gggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCGCGCCGAGCAGATAAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCCCAcc             >  1:754625/1‑149 (MQ=255)
                                                                                                                                      gAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCgg           <  2:3631207/149‑1 (MQ=255)
                                                                                                                                       aGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGa          <  2:3403349/149‑1 (MQ=255)
                                                                                                                                        gTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt         >  1:4160331/1‑149 (MQ=255)
                                                                                                                                        gTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt         >  1:3929254/1‑149 (MQ=255)
                                                                                                                                          gAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGa       >  1:1491429/1‑149 (MQ=255)
                                                                                                                                           aaGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAg      <  2:2408026/149‑1 (MQ=255)
                                                                                                                                              cAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTa   <  1:2475877/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAG  >  CP009273/546345‑546636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑