Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 607,931 G→A 100% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273607,9310GA99.6% 850.1 / ‑4.5 243S7F (TCC→TTC) fepAiron‑enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (114/128);  total (115/128)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607786‑608077
                                                                                                                                                 |                                                                                                                                                  
tGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaat                                                                                                                                                 >  2:1141426/1‑149 (MQ=255)
tGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaat                                                                                                                                                 <  2:4573740/149‑1 (MQ=255)
    gTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaatgaat                                                                                                                                             <  1:2373444/149‑1 (MQ=255)
     tGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtc                                                                                                                                            <  2:1642802/149‑1 (MQ=255)
      gATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtct                                                                                                                                           <  1:1014077/149‑1 (MQ=255)
        tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttc                                                                                                                                         >  2:1461900/1‑149 (MQ=255)
        tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttc                                                                                                                                         <  2:2535361/149‑1 (MQ=255)
        tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttc                                                                                                                                         >  2:3008022/1‑149 (MQ=255)
         ggTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttct                                                                                                                                        <  1:3123297/149‑1 (MQ=255)
           tCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTg                                                                                                                                      <  2:4008501/149‑1 (MQ=255)
            cGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGt                                                                                                                                     >  1:3050456/1‑149 (MQ=255)
            cGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAACCTTCTTGt                                                                                                                                     <  2:4657337/149‑1 (MQ=255)
              aaaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGACATGAATCTTCTTGTTc                                                                                                                                   >  1:3260425/1‑149 (MQ=255)
               aaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCa                                                                                                                                  >  1:361442/1‑149 (MQ=255)
                aCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAt                                                                                                                                 >  1:4327379/1‑149 (MQ=255)
                 cGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAtt                                                                                                                                <  1:1086674/149‑1 (MQ=255)
                 cGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAtt                                                                                                                                <  2:616387/149‑1 (MQ=255)
                   ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGt                                                                                                                              <  1:3894971/149‑1 (MQ=255)
                    cAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtt                                                                                                                             <  2:3393050/149‑1 (MQ=255)
                    cAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtt                                                                                                                             <  1:1726330/149‑1 (MQ=255)
                     aGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGttt                                                                                                                            >  1:408906/1‑149 (MQ=255)
                     aGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGttt                                                                                                                            <  2:2269313/149‑1 (MQ=255)
                      ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  1:4537154/1‑149 (MQ=255)
                            tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCt                                                                                                                     <  1:2429241/149‑1 (MQ=255)
                            tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCt                                                                                                                     <  1:3223084/149‑1 (MQ=255)
                                aGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAt                                                                                                                 <  2:3778092/149‑1 (MQ=255)
                                aGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAt                                                                                                                 <  2:2483108/149‑1 (MQ=255)
                                 gTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAtt                                                                                                                <  1:224960/149‑1 (MQ=255)
                                     tGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTg                                                                                                            >  2:468962/1‑149 (MQ=255)
                                       cTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGcc                                                                                                          <  2:322955/149‑1 (MQ=255)
                                        tCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                         >  1:3007675/1‑149 (MQ=255)
                                           gcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGa                                                                                                      <  1:515943/149‑1 (MQ=255)
                                             ggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAt                                                                                                    <  2:1064204/149‑1 (MQ=255)
                                              gcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAtt                                                                                                   <  2:2051139/149‑1 (MQ=255)
                                              gcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAtt                                                                                                   <  1:1618151/149‑1 (MQ=255)
                                                  tAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                                                               >  1:2749196/1‑149 (MQ=255)
                                                  tAACGACAATAGTATCGTCATGAGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                                                               <  1:2820878/149‑1 (MQ=255)
                                                      gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGt                                                                                           >  2:2317529/1‑149 (MQ=255)
                                                      gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGt                                                                                           <  2:3081154/149‑1 (MQ=255)
                                                       aCAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTc                                                                                          >  1:1116660/1‑149 (MQ=255)
                                                        cAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                         >  1:1839643/1‑149 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCgg                                                                                        >  1:3423931/1‑149 (MQ=255)
                                                          aTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCggg                                                                                       >  1:1475950/1‑149 (MQ=255)
                                                          aTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCggg                                                                                       >  1:3864964/1‑149 (MQ=255)
                                                          aTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCggg                                                                                       >  2:1551487/1‑149 (MQ=255)
                                                          aTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCggg                                                                                       >  1:910002/1‑149 (MQ=255)
                                                           tAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGc                                                                                      >  2:1810506/1‑149 (MQ=255)
                                                                   tCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgt                                                                              >  2:2623669/1‑149 (MQ=255)
                                                                   tCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgt                                                                              >  2:1609943/1‑149 (MQ=255)
                                                                    cATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgtc                                                                             <  2:116632/149‑1 (MQ=255)
                                                                      tgtgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtcat                                                                           <  1:2111893/149‑1 (MQ=255)
                                                                       gtgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtcatc                                                                          >  2:3745523/1‑149 (MQ=255)
                                                                         gAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCaa                                                                        >  2:891884/1‑149 (MQ=255)
                                                                            aCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATcaccac                                                                     >  2:3481927/1‑149 (MQ=255)
                                                                              aGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATACATCCCCAGATTGACCAACCAGGCCAGGAAATGAACCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacga                                                                   <  1:3519414/149‑1 (MQ=255)
                                                                              aGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacga                                                                   <  2:14290/149‑1 (MQ=255)
                                                                               ggAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgac                                                                  <  2:2440512/149‑1 (MQ=255)
                                                                                      cggtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATccc                                                           <  1:4445463/149‑1 (MQ=255)
                                                                                         tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCt                                                        >  2:3276590/1‑149 (MQ=255)
                                                                                         tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCt                                                        <  2:3307213/149‑1 (MQ=255)
                                                                                             ctctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACcg                                                    >  1:2609512/1‑149 (MQ=255)
                                                                                             ctctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACcg                                                    >  1:4662542/1‑149 (MQ=255)
                                                                                                    gCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAc                                             >  1:4679363/1‑149 (MQ=255)
                                                                                                     ccTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAcc                                            <  2:8530/149‑1 (MQ=255)
                                                                                                     ccTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAcc                                            >  2:3527110/1‑149 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCtt                                          <  1:545762/149‑1 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCtt                                          <  1:4492271/149‑1 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCtt                                          <  2:1263000/149‑1 (MQ=255)
                                                                                                            tACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATc                                     <  2:4424307/149‑1 (MQ=255)
                                                                                                             aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATcc                                    >  2:2163778/1‑149 (MQ=255)
                                                                                                             aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATcc                                    >  2:1841527/1‑149 (MQ=255)
                                                                                                               cccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGAAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAACTTTGATCCTg                                  >  2:4049145/1‑149 (MQ=255)
                                                                                                                ccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGa                                 >  2:2258593/1‑149 (MQ=255)
                                                                                                                ccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGa                                 <  2:2531643/149‑1 (MQ=255)
                                                                                                                  aTAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGaaa                               <  2:1501571/149‑1 (MQ=255)
                                                                                                                  aTAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGaaa                               >  1:1330347/1‑149 (MQ=255)
                                                                                                                   tAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAg                              >  1:3941388/1‑149 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  2:2791803/149‑1 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  2:4476673/149‑1 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  2:2077458/149‑1 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  1:626231/149‑1 (MQ=255)
                                                                                                                     aaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGac                            >  1:704097/1‑149 (MQ=255)
                                                                                                                     aaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCCACCGCGAAAACCTTTGATCCTGAAAGac                            <  1:370530/149‑1 (MQ=255)
                                                                                                                         cccAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgc                        >  2:3350251/1‑149 (MQ=255)
                                                                                                                           cAGATTGACCAACACGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcag                      <  2:203651/149‑1 (MQ=255)
                                                                                                                                  aCCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGtt               <  1:820268/149‑1 (MQ=255)
                                                                                                                                   ccAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTg              >  1:295569/1‑149 (MQ=255)
                                                                                                                                    caacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTgg             <  1:3733411/149‑1 (MQ=255)
                                                                                                                                    caacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTgg             <  2:2463249/149‑1 (MQ=255)
                                                                                                                                     aacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGt            >  1:3599345/1‑149 (MQ=255)
                                                                                                                                     aacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGt            >  1:901777/1‑149 (MQ=255)
                                                                                                                                      acaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtt           >  2:1190916/1‑149 (MQ=255)
                                                                                                                                      acaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtt           <  1:2893267/149‑1 (MQ=255)
                                                                                                                                      acaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtt           <  1:944362/149‑1 (MQ=255)
                                                                                                                                       caaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACTACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGgta          >  2:787213/1‑149 (MQ=255)
                                                                                                                                       caaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtta          >  2:787503/1‑149 (MQ=255)
                                                                                                                                       caaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtta          >  2:3178199/1‑149 (MQ=255)
                                                                                                                                        aaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaa         <  1:683021/149‑1 (MQ=255)
                                                                                                                                        aaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaa         <  2:1452115/149‑1 (MQ=255)
                                                                                                                                             cAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTtaattaat    >  1:1422649/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607786‑608077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑