Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 624,669 G→T 88.1% T221T (ACG→ACT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273624,6690GT88.1% 663.1 / 83.5 262T221T (ACG→ACT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/13);  new base T (104/126);  total (123/139)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624523‑624817
                                                                                                                                                  |                                                                                                                                                    
  gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                  <  2:3065718/149‑1 (MQ=255)
  gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                  >  2:3180673/1‑149 (MQ=255)
    cTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                                <  1:1365782/149‑1 (MQ=255)
     tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGt                                                                                                                                               >  1:3828436/1‑149 (MQ=255)
     tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGt                                                                                                                                               <  2:4463658/149‑1 (MQ=255)
      ggCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                              <  2:1710612/149‑1 (MQ=255)
      ggCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                              <  2:3996376/149‑1 (MQ=255)
       gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTc                                                                                                                                             >  1:558394/1‑149 (MQ=255)
       gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTc                                                                                                                                             <  2:4491805/149‑1 (MQ=255)
        cTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTcc                                                                                                                                            >  2:4106881/1‑149 (MQ=255)
         tCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCt                                                                                                                                           <  2:2608864/149‑1 (MQ=255)
          ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTc                                                                                                                                          <  1:2250264/149‑1 (MQ=255)
           caccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGACGACCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                         >  2:529040/1‑149 (MQ=255)
             ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGcc                                                                                                                                       <  1:2180948/149‑1 (MQ=255)
             ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGcc                                                                                                                                       <  2:2355198/149‑1 (MQ=255)
              cgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCct                                                                                                                                      <  1:4540756/149‑1 (MQ=255)
                acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctct                                                                                                                                    <  1:2596854/149‑1 (MQ=255)
                acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCctct                                                                                                                                    >  1:1248256/1‑149 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCt                                                                                                                               <  1:3012480/149‑1 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCt                                                                                                                               <  1:3674247/149‑1 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCt                                                                                                                               >  2:31451/1‑149 (MQ=255)
                         tGCAACGCACGCTGTGTGTGAGAGATGACGCCGAAGAACAGAGTATTCGCGGCTTTGGAGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTAGTTCCTCGCCTCTGACATCgcc                                                                                                                           >  2:4364929/1‑149 (MQ=255)
                         tGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgcc                                                                                                                           >  2:1937404/1‑149 (MQ=255)
                            aaCGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagc                                                                                                                        >  1:4680068/1‑149 (MQ=255)
                             acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                       >  1:44775/1‑149 (MQ=255)
                             acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCACCAACACGATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                       <  2:1433417/149‑1 (MQ=255)
                               gcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                     >  1:3399791/1‑149 (MQ=255)
                               gcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                     >  2:4316192/1‑149 (MQ=255)
                                     tgtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAcc                                                                                                               <  2:4179020/149‑1 (MQ=255)
                                         ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTAc                                                                                                           <  2:3725637/149‑1 (MQ=255)
                                              gCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                      <  2:1281909/149‑1 (MQ=255)
                                                gATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGatat                                                                                                    >  1:1136216/1‑149 (MQ=255)
                                                 aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                                   >  2:2266478/1‑149 (MQ=255)
                                                 aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                                   >  2:4784342/1‑149 (MQ=255)
                                                     cGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTgg                                                                                               <  1:4170894/149‑1 (MQ=255)
                                                       ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTc                                                                                             <  1:3488920/149‑1 (MQ=255)
                                                         gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                           >  2:2910642/1‑149 (MQ=255)
                                                         gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                           <  2:1586395/149‑1 (MQ=255)
                                                          aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                          >  2:1962897/1‑149 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        <  1:4087356/149‑1 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        <  2:665254/149‑1 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        >  2:1338843/1‑149 (MQ=255)
                                                                  cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                  <  1:39681/149‑1 (MQ=255)
                                                                  cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                  <  1:4312352/149‑1 (MQ=255)
                                                                   gTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCa                                                                                 >  1:1451831/1‑149 (MQ=255)
                                                                    tATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCaa                                                                                >  1:410408/1‑149 (MQ=255)
                                                                      ttCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                              >  2:1392810/1‑149 (MQ=255)
                                                                           ggCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggg                                                                         <  1:3290977/149‑1 (MQ=255)
                                                                            gCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgggg                                                                        >  1:2161781/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  2:4150535/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  2:277598/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  1:2338274/1‑149 (MQ=255)
                                                                                 ggCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATa                                                                   <  1:3323139/149‑1 (MQ=255)
                                                                                  gCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATaa                                                                  <  1:1558373/149‑1 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                >  1:644824/1‑149 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                <  1:2726731/149‑1 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                >  2:4287147/1‑149 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               <  2:3248070/149‑1 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               <  2:4671793/149‑1 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               >  1:734513/1‑149 (MQ=255)
                                                                                       cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATg                                                             <  2:3828436/149‑1 (MQ=255)
                                                                                       cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATg                                                             <  2:3611749/149‑1 (MQ=255)
                                                                                         gTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAt                                                           <  2:4547389/149‑1 (MQ=255)
                                                                                          tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATc                                                          <  2:515094/149‑1 (MQ=255)
                                                                                              aaCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGa                                                      >  1:3790234/1‑149 (MQ=255)
                                                                                               aCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                     >  1:3219322/1‑149 (MQ=255)
                                                                                               aCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                     <  2:4680068/149‑1 (MQ=255)
                                                                                                cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                    <  2:1660957/149‑1 (MQ=255)
                                                                                                cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                    <  1:4535/149‑1 (MQ=255)
                                                                                                    gCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGcc                                                >  1:3629954/1‑149 (MQ=255)
                                                                                                         ccGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTa                                           >  2:2689080/1‑149 (MQ=255)
                                                                                                           gCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAc                                         >  1:1827136/1‑149 (MQ=255)
                                                                                                              ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                      >  2:1361683/1‑149 (MQ=255)
                                                                                                              ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                      >  1:815865/1‑149 (MQ=255)
                                                                                                                ggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGACACGCCATTTAACGCTcg                                    >  2:1015879/1‑149 (MQ=255)
                                                                                                                 gAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                                   >  2:1387012/1‑149 (MQ=255)
                                                                                                                  aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgac                                  <  2:709015/149‑1 (MQ=255)
                                                                                                                  aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgac                                  <  2:708001/149‑1 (MQ=255)
                                                                                                                     aTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGaa                               >  2:150489/1‑149 (MQ=255)
                                                                                                                       cGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACt                             >  1:2915199/1‑149 (MQ=255)
                                                                                                                        gCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                            <  2:3540897/149‑1 (MQ=255)
                                                                                                                        gCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                            <  1:1703702/149‑1 (MQ=255)
                                                                                                                         cccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGa                           >  1:2408284/1‑149 (MQ=255)
                                                                                                                           cGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                         >  1:1244469/1‑149 (MQ=255)
                                                                                                                           cGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCCGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                         >  1:3108830/1‑149 (MQ=255)
                                                                                                                             tCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGc                       <  2:2397677/149‑1 (MQ=255)
                                                                                                                               cacaAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGcca                     >  1:2825560/1‑149 (MQ=255)
                                                                                                                                acaAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac                    >  2:303036/1‑149 (MQ=255)
                                                                                                                                   agagATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg                 >  2:2892830/1‑149 (MQ=255)
                                                                                                                                   agagATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg                 >  1:88635/1‑149 (MQ=255)
                                                                                                                                    gagaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGc                >  1:1078551/1‑149 (MQ=255)
                                                                                                                                     agaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCTCCTGCCATATTACCCTACAGGATATTGTGGTCGATGGCTGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACTCTCGACGAACTGAACGCCACCAGCg               <  2:3534608/149‑1 (MQ=255)
                                                                                                                                       aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt             <  1:3424845/149‑1 (MQ=255)
                                                                                                                                       aTCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt             <  2:1814609/149‑1 (MQ=255)
                                                                                                                                        tCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATa            >  1:2094420/1‑149 (MQ=255)
                                                                                                                                           ccAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAaca         <  2:2183816/149‑1 (MQ=255)
                                                                                                                                            cAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAacac        <  1:3523184/149‑1 (MQ=255)
                                                                                                                                            cAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAacac        <  2:2140238/149‑1 (MQ=255)
                                                                                                                                             aaCACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca       <  2:867570/149‑1 (MQ=255)
                                                                                                                                              acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATCTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa      >  2:3703719/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624523‑624817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑