Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 627,428 C→A 85.9% I693I (ATC→ATA)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273627,4280CA85.9% 644.0 / 119.0 285I693I (ATC→ATA) cstAcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (18/22);  new base A (108/137);  total (126/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 285 total aligned reads displayed.

GATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAG  >  CP009273/627282‑627574
                                                                                                                                                  |                                                                                                                                                  
gATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGt                                                                                                                                                  <  1:1581612/149‑1 (MQ=255)
 aTGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGACGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTg                                                                                                                                                 <  1:2019588/149‑1 (MQ=255)
  tGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCAGTTTAGTATTTTAAGACGGAACTGGCGGCATTTAATGATCCGAAGCAAACGGAGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTgg                                                                                                                                                <  1:2441971/149‑1 (MQ=255)
  tGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTgg                                                                                                                                                >  2:4348288/1‑149 (MQ=255)
           aaCCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaa                                                                                                                                       >  2:1828373/1‑149 (MQ=255)
            aCCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                      <  1:3007377/149‑1 (MQ=255)
            aCCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                      >  2:1637134/1‑149 (MQ=255)
             ccATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCaaaa                                                                                                                                     >  1:1021015/1‑149 (MQ=255)
               aTCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAgg                                                                                                                                   >  2:4247498/1‑149 (MQ=255)
               aTCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAgg                                                                                                                                   >  2:2492766/1‑149 (MQ=255)
                   tcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGca                                                                                                                               <  1:1914099/149‑1 (MQ=255)
                   tcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGca                                                                                                                               <  2:3015483/149‑1 (MQ=255)
                    cttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGcac                                                                                                                              >  2:4002284/1‑149 (MQ=255)
                     tttATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGcaca                                                                                                                             >  1:2050602/1‑149 (MQ=255)
                        aTGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTa                                                                                                                          <  1:1787616/149‑1 (MQ=255)
                          ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAACCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaaa                                                                                                                        <  1:419522/149‑1 (MQ=255)
                           gtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAg                                                                                                                       <  2:1332293/149‑1 (MQ=255)
                               tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCag                                                                                                                   >  1:2524835/1‑149 (MQ=255)
                                cGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCaga                                                                                                                  <  2:2124482/149‑1 (MQ=255)
                                 gtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                                 <  1:371672/149‑1 (MQ=255)
                                 gtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                                 <  1:3835897/149‑1 (MQ=255)
                                     tggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCtgagtgag                                                                                                             <  2:4744937/149‑1 (MQ=255)
                                     tggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                             >  2:3339323/1‑149 (MQ=255)
                                     tggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                             >  1:690810/1‑149 (MQ=255)
                                        ttCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggg                                                                                                          <  2:286906/149‑1 (MQ=255)
                                         tCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGG‑AAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggggg                                                                                                        <  1:2763005/149‑1 (MQ=255)
                                             gCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAt                                                                                                     <  1:259778/149‑1 (MQ=255)
                                             gCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAt                                                                                                     <  1:2085562/149‑1 (MQ=255)
                                              cACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATg                                                                                                    <  1:3080563/149‑1 (MQ=255)
                                                cTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGtt                                                                                                  >  2:891892/1‑149 (MQ=255)
                                                 tGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTg                                                                                                 <  1:1492059/149‑1 (MQ=255)
                                                  gTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTgg                                                                                                <  2:3295379/149‑1 (MQ=255)
                                                    tctcGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCg                                                                                              >  1:2889385/1‑149 (MQ=255)
                                                       cGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAAt                                                                                           <  1:621131/149‑1 (MQ=255)
                                                       cGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAAt                                                                                           >  1:3361944/1‑149 (MQ=255)
                                                         aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGt                                                                                         <  1:4761903/149‑1 (MQ=255)
                                                          ttAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                        >  1:4415392/1‑149 (MQ=255)
                                                             aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                     >  2:1132260/1‑149 (MQ=255)
                                                               aCGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCtt                                                                                   <  2:837845/149‑1 (MQ=255)
                                                                 ggCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                                 <  1:1008285/149‑1 (MQ=255)
                                                                  gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGt                                                                                >  1:1100430/1‑149 (MQ=255)
                                                                    aCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGc                                                                              >  1:75862/1‑149 (MQ=255)
                                                                     cTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGcc                                                                             <  2:106646/149‑1 (MQ=255)
                                                                       tGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                                                                           <  2:445562/149‑1 (MQ=255)
                                                                        gCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAg                                                                          <  2:1423710/149‑1 (MQ=255)
                                                                        gCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAg                                                                          <  2:2241285/149‑1 (MQ=255)
                                                                        gCGGCAATGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAg                                                                          <  2:3972216/149‑1 (MQ=255)
                                                                           gCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                                       >  2:3215076/1‑149 (MQ=255)
                                                                           gCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                                       >  1:4584108/1‑149 (MQ=255)
                                                                            cATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGtt                                                                      <  1:1711238/149‑1 (MQ=255)
                                                                             aTTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTc                                                                     >  2:737580/1‑149 (MQ=255)
                                                                              ttGAAAGATCCGACGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                    <  1:898207/149‑1 (MQ=255)
                                                                                 aaaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc                                                                 <  2:3668574/149‑1 (MQ=255)
                                                                                  aaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                                >  1:1319307/1‑149 (MQ=255)
                                                                                   aGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCtctc                                                               >  2:2658620/1‑149 (MQ=255)
                                                                                     aTCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                             >  2:2736359/1‑149 (MQ=255)
                                                                                      tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                            >  1:2348650/1‑149 (MQ=255)
                                                                                        cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCt                                                          >  1:4559860/1‑149 (MQ=255)
                                                                                         gAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTa                                                         >  1:844943/1‑149 (MQ=255)
                                                                                         gAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTa                                                         >  1:4117247/1‑149 (MQ=255)
                                                                                            gCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                      >  2:367234/1‑149 (MQ=255)
                                                                                             ccAACGGCGAAAGACACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAccc                                                     <  1:4010525/149‑1 (MQ=255)
                                                                                               aaCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctc                                                   <  2:3322691/149‑1 (MQ=255)
                                                                                               aaCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctc                                                   >  1:3948203/1‑149 (MQ=255)
                                                                                                aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                                  <  2:598830/149‑1 (MQ=255)
                                                                                                aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                                  <  2:1523098/149‑1 (MQ=255)
                                                                                                   gCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAGGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTccc                                               <  1:1563289/149‑1 (MQ=255)
                                                                                                    cGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTcccc                                              <  1:3243401/149‑1 (MQ=255)
                                                                                                       aagaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGt                                           <  1:4086250/149‑1 (MQ=255)
                                                                                                        agaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTg                                          >  1:3487235/1‑149 (MQ=255)
                                                                                                         gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                                         >  1:4282949/1‑149 (MQ=255)
                                                                                                          aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                                        >  2:2308526/1‑149 (MQ=255)
                                                                                                          aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                                        >  2:12739/1‑149 (MQ=255)
                                                                                                           aaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                                       >  2:761161/1‑149 (MQ=255)
                                                                                                                 gTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAGGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCCGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                                 >  1:4623737/1‑149 (MQ=255)
                                                                                                                 gTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                                 <  2:4197481/149‑1 (MQ=255)
                                                                                                                 gTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                                 <  2:168062/149‑1 (MQ=255)
                                                                                                                    tGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGAc                              <  2:2746956/149‑1 (MQ=255)
                                                                                                                        ccAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc                          <  2:4583966/149‑1 (MQ=255)
                                                                                                                        ccAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc                          <  1:1941264/149‑1 (MQ=255)
                                                                                                                          aaTGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCg                        >  1:2705649/1‑149 (MQ=255)
                                                                                                                          aaTGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCg                        >  1:3247055/1‑149 (MQ=255)
                                                                                                                           aTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGa                       >  1:3288418/1‑149 (MQ=255)
                                                                                                                            tGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTGCCAAATCACCATAACCAAAACCCCGGTGGGGCGAGGGGAATGACCgtg                      >  2:2728115/1‑147 (MQ=255)
                                                                                                                             gCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgc                     >  2:1967477/1‑149 (MQ=255)
                                                                                                                             gCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGGTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgc                     <  1:2182369/149‑1 (MQ=255)
                                                                                                                               cGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcgc                   >  1:576880/1‑149 (MQ=255)
                                                                                                                                ggAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                  >  2:669869/1‑149 (MQ=255)
                                                                                                                                 gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt                 >  2:2439634/1‑149 (MQ=255)
                                                                                                                                  aaaaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt                <  2:3513741/149‑1 (MQ=255)
                                                                                                                                    aaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa              <  1:546572/149‑1 (MQ=255)
                                                                                                                                      tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATa            >  1:1968648/1‑149 (MQ=255)
                                                                                                                                      tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATa            <  2:3753292/149‑1 (MQ=255)
                                                                                                                                       gTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAg           >  1:3587284/1‑149 (MQ=255)
                                                                                                                                          gaggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAt        >  2:3131912/1‑149 (MQ=255)
                                                                                                                                           aggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAtt       <  2:573349/149‑1 (MQ=255)
                                                                                                                                           aggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAtt       <  1:1616558/149‑1 (MQ=255)
                                                                                                                                            ggagATAGTGGCGCAGGCAACAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt      <  2:4517099/149‑1 (MQ=255)
                                                                                                                                            ggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGGTGATAGCAtgt      >  2:4249105/1‑147 (MQ=255)
                                                                                                                                                aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAg  <  1:4348288/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAG  >  CP009273/627282‑627574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑