Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 639,231 G→A 100% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273639,2310GA99.4% 1241.3 / ‑2.7 358L65F (CTT→TTT) rnkregulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (165/191);  total (166/192)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 358 total aligned reads displayed.

GCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCG  >  CP009273/639085‑639376
                                                                                                                                                  |                                                                                                                                                 
gccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                 >  2:1166572/1‑149 (MQ=255)
gccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                 <  1:3479349/149‑1 (MQ=255)
 cccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAtt                                                                                                                                                <  1:3567647/149‑1 (MQ=255)
  ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATTGCTAAAATTg                                                                                                                                               >  2:1829141/1‑149 (MQ=255)
  ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                               <  1:3671237/149‑1 (MQ=255)
  ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                               >  1:3502842/1‑149 (MQ=255)
  ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                               >  2:2605592/1‑149 (MQ=255)
    ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCg                                                                                                                                             >  2:568143/1‑149 (MQ=255)
    ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCg                                                                                                                                             <  1:2111570/149‑1 (MQ=255)
     gAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCgg                                                                                                                                            >  1:321309/1‑149 (MQ=255)
     gAAGGTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCgg                                                                                                                                            <  2:1305997/149‑1 (MQ=255)
      aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGa                                                                                                                                           >  1:2936532/1‑149 (MQ=255)
       aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                          >  1:4615095/1‑149 (MQ=255)
        gTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAt                                                                                                                                         >  2:1603563/1‑149 (MQ=255)
         ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAtt                                                                                                                                        <  1:824875/149‑1 (MQ=255)
          tCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                       >  1:2034882/1‑149 (MQ=255)
           cccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTa                                                                                                                                      <  2:1431739/149‑1 (MQ=255)
            ccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTaa                                                                                                                                     <  2:3674994/149‑1 (MQ=255)
              aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGTCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAcc                                                                                                                                   <  1:4774346/149‑1 (MQ=255)
              aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAcc                                                                                                                                   <  1:2362388/149‑1 (MQ=255)
                tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCg                                                                                                                                 <  1:1704370/149‑1 (MQ=255)
                   aTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCTGCGTGCTCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCt                                                                                                                              <  2:2459488/149‑1 (MQ=255)
                   aTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAGTTAACCCGGCt                                                                                                                              >  2:2869805/1‑149 (MQ=255)
                    tGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTg                                                                                                                             <  1:1170399/149‑1 (MQ=255)
                    tGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTg                                                                                                                             <  2:3708364/149‑1 (MQ=255)
                      gAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGtt                                                                                                                           >  2:1556484/1‑149 (MQ=255)
                         tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                        <  2:2152474/149‑1 (MQ=255)
                         tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                        >  1:3925823/1‑149 (MQ=255)
                         tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                        <  1:4062781/149‑1 (MQ=255)
                               aCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcac                                                                                                                  >  2:374064/1‑149 (MQ=255)
                                 gcgcAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcacca                                                                                                                <  2:2250228/149‑1 (MQ=255)
                                      gCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                           <  1:794130/149‑1 (MQ=255)
                                      gCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                           >  1:4023633/1‑149 (MQ=255)
                                        cccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                         <  2:839045/149‑1 (MQ=255)
                                            gcagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGc                                                                                                     >  2:1729090/1‑149 (MQ=255)
                                             cagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCa                                                                                                    <  2:2696954/149‑1 (MQ=255)
                                             cagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCa                                                                                                    >  1:4522480/1‑149 (MQ=255)
                                              agTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAt                                                                                                   >  1:2859290/1‑149 (MQ=255)
                                                tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                 <  1:3050781/149‑1 (MQ=255)
                                                    gCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttc                                                                                             <  2:3397883/149‑1 (MQ=255)
                                                     cACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttct                                                                                            <  1:4460245/149‑1 (MQ=255)
                                                     cACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttct                                                                                            <  1:4352802/149‑1 (MQ=255)
                                                       ccTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTgg                                                                                          <  1:2072851/149‑1 (MQ=255)
                                                          aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGa                                                                                       <  2:2331376/149‑1 (MQ=255)
                                                          aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGa                                                                                       >  2:302419/1‑149 (MQ=255)
                                                           ccGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGGGTGGCAGCTCTTCTGGCGaa                                                                                      >  2:2920462/1‑149 (MQ=255)
                                                               aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  >  2:905680/1‑149 (MQ=255)
                                                               aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  <  1:1932114/149‑1 (MQ=255)
                                                               aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  >  1:1586216/1‑149 (MQ=255)
                                                               aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  <  1:985035/149‑1 (MQ=255)
                                                               aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAcaca                                                                                  <  1:3072497/149‑1 (MQ=255)
                                                                 ccATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                                <  2:4237500/149‑1 (MQ=255)
                                                                  cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAttt                                                                               <  1:3842420/149‑1 (MQ=255)
                                                                   aTAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGACGGATACACCAGCGTGAGCACACGCTCTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGTACACATTTg                                                                              <  2:2298419/149‑1 (MQ=255)
                                                                      aCGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgc                                                                           >  2:2190320/1‑149 (MQ=255)
                                                                           aaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGAt                                                                      >  1:598442/1‑149 (MQ=255)
                                                                             gCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATcc                                                                    <  2:4009590/149‑1 (MQ=255)
                                                                                gAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGACACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAAc                                                                 >  2:4470248/1‑149 (MQ=255)
                                                                                  gTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                               <  1:558299/149‑1 (MQ=255)
                                                                                    aTTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTg                                                             <  1:2312783/149‑1 (MQ=255)
                                                                                     ttGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGc                                                            >  1:4150910/1‑149 (MQ=255)
                                                                                     ttGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGc                                                            >  2:3944039/1‑149 (MQ=255)
                                                                                        cTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGtt                                                         <  1:3280438/149‑1 (MQ=255)
                                                                                        cTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGtt                                                         >  2:4130997/1‑149 (MQ=255)
                                                                                          aTCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTa                                                       >  1:990838/1‑149 (MQ=255)
                                                                                          aTCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTa                                                       >  2:2773201/1‑149 (MQ=255)
                                                                                           tCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTaa                                                      <  2:2093034/149‑1 (MQ=255)
                                                                                           tCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTaa                                                      >  2:4287192/1‑149 (MQ=255)
                                                                                             ggTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCACCCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcg                                                    >  2:1888076/1‑149 (MQ=255)
                                                                                                 aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGTCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                                >  2:3432833/1‑149 (MQ=255)
                                                                                                   ttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCgg                                              <  2:387481/149‑1 (MQ=255)
                                                                                                   ttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCgg                                              <  2:1262133/149‑1 (MQ=255)
                                                                                                    tttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGc                                             >  2:208817/1‑149 (MQ=255)
                                                                                                    tgtGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGc                                             <  2:4458609/147‑1 (MQ=255)
                                                                                                     ttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCg                                            >  1:3763947/1‑149 (MQ=255)
                                                                                                      tGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGa                                           >  1:2847191/1‑149 (MQ=255)
                                                                                                       gCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                          >  1:433600/1‑149 (MQ=255)
                                                                                                       gCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                          <  2:703052/149‑1 (MQ=255)
                                                                                                        ccGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAtt                                         <  1:2318405/149‑1 (MQ=255)
                                                                                                        ccGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAtt                                         >  2:3323458/1‑149 (MQ=255)
                                                                                                          ggATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTgg                                       <  1:2155828/149‑1 (MQ=255)
                                                                                                           gATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGc                                      >  1:4586922/1‑149 (MQ=255)
                                                                                                            aTACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTAGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                     >  2:4288961/1‑149 (MQ=255)
                                                                                                              acacCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaaa                                   >  1:3908209/1‑149 (MQ=255)
                                                                                                                acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAAcc                                 <  2:2503165/149‑1 (MQ=255)
                                                                                                                 ccAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                                >  1:1287762/1‑149 (MQ=255)
                                                                                                                   aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                              >  1:1004276/1‑149 (MQ=255)
                                                                                                                      gTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATa                           <  2:4319360/149‑1 (MQ=255)
                                                                                                                        gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAgg                         >  1:2690045/1‑149 (MQ=255)
                                                                                                                        gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAgg                         >  2:4604559/1‑149 (MQ=255)
                                                                                                                          gcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCg                       <  1:1418050/149‑1 (MQ=255)
                                                                                                                           cacaCGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCgg                      <  2:794939/149‑1 (MQ=255)
                                                                                                                                   cTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTcc              <  2:450646/149‑1 (MQ=255)
                                                                                                                                        ccATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCGTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagcag         <  1:713751/149‑1 (MQ=255)
                                                                                                                                         cATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa        >  2:1420832/1‑149 (MQ=255)
                                                                                                                                          aTCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGaa       <  2:3182044/149‑1 (MQ=255)
                                                                                                                                             gCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAatat    >  1:2568604/1‑149 (MQ=255)
                                                                                                                                              cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATc   <  1:3682244/149‑1 (MQ=255)
                                                                                                                                              cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATc   <  2:4522480/149‑1 (MQ=255)
                                                                                                                                              cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATc   <  2:3061000/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCG  >  CP009273/639085‑639376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑