Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,108 A→T 100% V522V (GTT→GTA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,1080AT99.1% 1085.9 / ‑0.6 314V522V (GTT→GTA) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (167/144);  total (168/146)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 314 total aligned reads displayed.

TGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT  >  CP009273/661962‑662253
                                                                                                                                                  |                                                                                                                                                 
tgGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTac                                                                                                                                                 <  1:4459951/149‑1 (MQ=255)
tgGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTac                                                                                                                                                 >  2:1419899/1‑149 (MQ=255)
 ggTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTaca                                                                                                                                                <  1:1615124/149‑1 (MQ=255)
 ggTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTaca                                                                                                                                                <  2:2491689/149‑1 (MQ=255)
  gTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTacac                                                                                                                                               >  2:3483749/1‑149 (MQ=255)
    cACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCg                                                                                                                                             >  2:1074526/1‑149 (MQ=255)
      cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTa                                                                                                                                           <  1:3943994/149‑1 (MQ=255)
      cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTa                                                                                                                                           <  1:1497877/149‑1 (MQ=255)
      cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTa                                                                                                                                           <  1:3327801/149‑1 (MQ=255)
      cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTa                                                                                                                                           <  1:3139011/149‑1 (MQ=255)
       gTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                          <  2:739596/149‑1 (MQ=255)
       gTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                          >  1:2618060/1‑149 (MQ=255)
        tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                         >  1:371889/1‑149 (MQ=255)
        tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                         >  1:1776562/1‑149 (MQ=255)
        tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                         <  2:3044281/149‑1 (MQ=255)
         aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                        >  1:4012730/1‑149 (MQ=255)
         aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                        <  1:3196536/149‑1 (MQ=255)
         aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                        <  1:624622/149‑1 (MQ=255)
          aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                       >  2:1909655/1‑149 (MQ=255)
            cGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAcc                                                                                                                                     >  1:2327545/1‑149 (MQ=255)
                cGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCt                                                                                                                                 >  2:1742617/1‑149 (MQ=255)
                cGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCt                                                                                                                                 <  2:4748191/149‑1 (MQ=255)
                   cAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTc                                                                                                                              <  2:2951381/149‑1 (MQ=255)
                     aTTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                            >  2:2733227/1‑149 (MQ=255)
                      ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                           >  2:2555238/1‑149 (MQ=255)
                          gtgCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCt                                                                                                                       >  2:465160/1‑149 (MQ=255)
                          gtgCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCt                                                                                                                       >  2:603513/1‑149 (MQ=255)
                           tgCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTc                                                                                                                      >  1:4647188/1‑149 (MQ=255)
                                aTTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGt                                                                                                                 >  1:4747115/1‑149 (MQ=255)
                                  tataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTaa                                                                                                               >  1:998722/1‑149 (MQ=255)
                                    taGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAcc                                                                                                             >  1:638646/1‑149 (MQ=255)
                                    taGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAcc                                                                                                             <  2:2614756/149‑1 (MQ=255)
                                     aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            >  2:838998/1‑149 (MQ=255)
                                        tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                         >  2:2708945/1‑149 (MQ=255)
                                        tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                         >  2:317280/1‑149 (MQ=255)
                                         ttcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaat                                                                                                        <  1:4000349/149‑1 (MQ=255)
                                           cgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatga                                                                                                      >  2:3245889/1‑149 (MQ=255)
                                            gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                     <  1:1696903/149‑1 (MQ=255)
                                            gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                     >  1:2455746/1‑149 (MQ=255)
                                            gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                     <  1:508401/149‑1 (MQ=255)
                                              tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                   <  1:1402312/149‑1 (MQ=255)
                                               cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  >  2:467669/1‑149 (MQ=255)
                                               cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  >  1:1118970/1‑149 (MQ=255)
                                               cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  <  1:2211486/149‑1 (MQ=255)
                                                     cAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                            <  2:281159/149‑1 (MQ=255)
                                                       gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                          >  2:1641297/1‑149 (MQ=255)
                                                       gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                          <  2:2387279/149‑1 (MQ=255)
                                                        aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACgg                                                                                         >  2:1246282/1‑149 (MQ=255)
                                                           gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCg                                                                                      >  2:2977426/1‑149 (MQ=255)
                                                           gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCg                                                                                      >  2:2091943/1‑149 (MQ=255)
                                                            aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                     <  1:415916/149‑1 (MQ=255)
                                                                ccTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCa                                                                                 <  1:1840749/149‑1 (MQ=255)
                                                                ccTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACAGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCa                                                                                 <  1:4423977/149‑1 (MQ=255)
                                                                  tGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATg                                                                               <  1:3978285/149‑1 (MQ=255)
                                                                      cGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGc                                                                           >  1:2115127/1‑149 (MQ=255)
                                                                          aCCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTa                                                                       >  2:2346763/1‑149 (MQ=255)
                                                                           ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                      >  1:3190096/1‑149 (MQ=255)
                                                                                  tCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGc                                                               >  1:3764002/1‑149 (MQ=255)
                                                                                    gccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAc                                                             >  1:484386/1‑149 (MQ=255)
                                                                                      cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                           <  1:2557046/149‑1 (MQ=255)
                                                                                       gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                          <  2:4389290/149‑1 (MQ=255)
                                                                                       gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                          >  2:4596226/1‑149 (MQ=255)
                                                                                       gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                          <  2:3751841/149‑1 (MQ=255)
                                                                                        cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGt                                                         <  1:3003298/149‑1 (MQ=255)
                                                                                          aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                       <  2:180086/149‑1 (MQ=255)
                                                                                          aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                       >  1:4136009/1‑149 (MQ=255)
                                                                                             ttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGcc                                                    <  2:1668029/149‑1 (MQ=255)
                                                                                                tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTc                                                 <  2:3813037/149‑1 (MQ=255)
                                                                                                tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTc                                                 >  2:539055/1‑149 (MQ=255)
                                                                                                  cGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCtt                                               <  2:3493790/149‑1 (MQ=255)
                                                                                                   ggTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTc                                              >  1:4056521/1‑149 (MQ=255)
                                                                                                   ggTGCGCTAGCAAAGTATGTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCCGTACCCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTc                                              <  2:1061937/149‑1 (MQ=255)
                                                                                                    gTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                             >  1:2022022/1‑149 (MQ=255)
                                                                                                         cTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGt                                        >  2:758570/1‑149 (MQ=255)
                                                                                                          tAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                       >  2:4572073/1‑149 (MQ=255)
                                                                                                           aGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGc                                      >  2:766830/1‑149 (MQ=255)
                                                                                                                 gTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatca                                <  1:1003477/149‑1 (MQ=255)
                                                                                                                    tttATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCa                             >  1:1963190/1‑149 (MQ=255)
                                                                                                                    tttATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCa                             >  2:1325979/1‑149 (MQ=255)
                                                                                                                    tttATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCa                             >  1:3149219/1‑149 (MQ=255)
                                                                                                                         gcgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCACATGa                        >  2:615334/1‑149 (MQ=255)
                                                                                                                         gcgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGa                        <  2:4285436/149‑1 (MQ=255)
                                                                                                                          cgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAg                       <  2:3954680/149‑1 (MQ=255)
                                                                                                                           gcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                      <  1:2744165/149‑1 (MQ=255)
                                                                                                                            ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGa                     >  2:4777552/1‑149 (MQ=255)
                                                                                                                            ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGa                     >  2:1714405/1‑149 (MQ=255)
                                                                                                                            ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGa                     >  2:4103218/1‑149 (MQ=255)
                                                                                                                             cGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                    <  1:3405283/149‑1 (MQ=255)
                                                                                                                                 ccGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt                <  1:4365660/149‑1 (MQ=255)
                                                                                                                                    ttAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAc             <  1:4448562/149‑1 (MQ=255)
                                                                                                                                    ttAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAc             <  2:3158367/149‑1 (MQ=255)
                                                                                                                                     tAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACg            >  1:2806406/1‑149 (MQ=255)
                                                                                                                                       gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt          <  2:2079506/149‑1 (MQ=255)
                                                                                                                                         gCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATAc        <  1:65470/149‑1 (MQ=255)
                                                                                                                                         gCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATAc        <  1:294339/149‑1 (MQ=255)
                                                                                                                                         gCGGTTAGCTACACCGTACATACCGTCGTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATAc        <  2:1475717/149‑1 (MQ=255)
                                                                                                                                             ttAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTc    <  1:3470100/149‑1 (MQ=255)
                                                                                                                                             ttAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTc    <  1:603513/149‑1 (MQ=255)
                                                                                                                                             ttAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTc    <  1:2970686/149‑1 (MQ=255)
                                                                                                                                               aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAt  >  1:1841360/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT  >  CP009273/661962‑662253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑