BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	693,739	G→T	100%	H299N (CAC→AAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	693,739	0	G	T	100.0%	813.0 / NA	233	H299N (CAC→AAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (143/90); total (143/90)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCG  >  CP009273/693593‑693884

ggACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAgtgaagttaa                                                                                                                                                 <  2:143461/149‑1 (MQ=255)

 gACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAt                                                                                                                                                >  1:3159928/1‑149 (MQ=255)

   cAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAttt                                                                                                                                              <  2:526286/149‑1 (MQ=255)

    aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTc                                                                                                                                             <  2:196373/149‑1 (MQ=255)

     gCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                            >  1:4503956/1‑149 (MQ=255)

        ccATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGa                                                                                                                                         <  2:848770/149‑1 (MQ=255)

        ccATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGa                                                                                                                                         <  1:355668/149‑1 (MQ=255)

          aTTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATg                                                                                                                                       >  1:4243063/1‑149 (MQ=255)

            tttAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCa                                                                                                                                     <  1:4116701/149‑1 (MQ=255)

            tttAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCa                                                                                                                                     >  2:4122853/1‑149 (MQ=255)

              tAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAcc                                                                                                                                   <  2:867260/149‑1 (MQ=255)

               aaTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCg                                                                                                                                  <  2:1767241/149‑1 (MQ=255)

                aTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGt                                                                                                                                 >  1:1522044/1‑149 (MQ=255)

                 tGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTg                                                                                                                                >  2:129315/1‑149 (MQ=255)

                    ccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                             >  1:384947/1‑149 (MQ=255)

                    ccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                             <  1:4642950/149‑1 (MQ=255)

                        cGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAt                                                                                                                         >  2:4438743/1‑149 (MQ=255)

                        cGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAt                                                                                                                         <  1:1680435/149‑1 (MQ=255)

                          ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                       >  2:3627253/1‑149 (MQ=255)

                          ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                       >  2:3372052/1‑149 (MQ=255)

                          ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                       >  2:323169/1‑149 (MQ=255)

                          ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATgg                                                                                                                       >  1:6249/1‑149 (MQ=255)

                           cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                      <  2:4207858/149‑1 (MQ=255)

                           cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                      <  1:3961460/149‑1 (MQ=255)

                           cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                      <  2:4660709/149‑1 (MQ=255)

                            ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGtt                                                                                                                     >  2:4578603/1‑149 (MQ=255)

                               aTCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGc                                                                                                                  <  1:4731673/149‑1 (MQ=255)

                               aTCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGc                                                                                                                  >  1:1182309/1‑149 (MQ=255)

                                   tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACt                                                                                                              >  2:1672951/1‑149 (MQ=255)

                                   tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACt                                                                                                              <  2:2819681/149‑1 (MQ=255)

                                   tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACt                                                                                                              <  1:4273152/149‑1 (MQ=255)

                                   tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACt                                                                                                              <  2:2819841/149‑1 (MQ=255)

                                    aCGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                             >  1:3378366/1‑149 (MQ=255)

                                    aCGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                             <  1:1060450/149‑1 (MQ=255)

                                    aCGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                             <  1:4218132/149‑1 (MQ=255)

                                    aCGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAGGGTTTGCCACtt                                                                                                             >  1:988874/1‑149 (MQ=255)

                                      gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTcc                                                                                                           >  2:552274/1‑149 (MQ=255)

                                      gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTcc                                                                                                           >  1:1023411/1‑149 (MQ=255)

                                        gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTg                                                                                                         >  1:726922/1‑149 (MQ=255)

                                        gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAACGTGACCAGATGGTTTGCCACTTCCTg                                                                                                         >  1:3677828/1‑149 (MQ=255)

                                          cATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTggg                                                                                                       >  1:2966856/1‑149 (MQ=255)

                                          cATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTggg                                                                                                       >  2:3768977/1‑149 (MQ=255)

                                           aTTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgc                                                                                                      <  1:4279025/149‑1 (MQ=255)

                                           aTTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgc                                                                                                      >  2:3681276/1‑149 (MQ=255)

                                            ttAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgct                                                                                                     <  1:813210/149‑1 (MQ=255)

                                               aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                  <  2:232208/149‑1 (MQ=255)

                                               aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                  >  1:161958/1‑149 (MQ=255)

                                               aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                  >  1:4621598/1‑149 (MQ=255)

                                                aTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCtt                                                                                                 <  1:413015/149‑1 (MQ=255)

                                                 tACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCttt                                                                                                >  1:2200932/1‑149 (MQ=255)

                                                 tACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCttt                                                                                                >  1:2200399/1‑149 (MQ=255)

                                                     tCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGa                                                                                            >  1:3928127/1‑149 (MQ=255)

                                                      cGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGAt                                                                                           <  2:1328234/149‑1 (MQ=255)

                                                      cGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGAt                                                                                           >  2:3113082/1‑149 (MQ=255)

                                                              aaGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGaa                                                                                   <  2:4315503/149‑1 (MQ=255)

                                                               aGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAc                                                                                  <  2:1620579/149‑1 (MQ=255)

                                                                  gcgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCg                                                                               <  2:1048664/149‑1 (MQ=255)

                                                                    gAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGc                                                                             <  1:3760101/149‑1 (MQ=255)

                                                                    gAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGc                                                                             <  2:3323036/149‑1 (MQ=255)

                                                                     aaTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCa                                                                            <  2:1394582/149‑1 (MQ=255)

                                                                     aaTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCa                                                                            >  2:2635396/1‑149 (MQ=255)

                                                                      aTAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAg                                                                           >  1:1857172/1‑149 (MQ=255)

                                                                         gTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                        >  1:4589481/1‑149 (MQ=255)

                                                                         gTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                        >  2:2185880/1‑149 (MQ=255)

                                                                         gTGGTCAAATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                        >  1:2757852/1‑149 (MQ=255)

                                                                            gTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAc                                                                     >  1:1009887/1‑149 (MQ=255)

                                                                             tCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACa                                                                    <  1:2830679/149‑1 (MQ=255)

                                                                               acaTCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGc                                                                  >  2:2904601/1‑149 (MQ=255)

                                                                                  tcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCaaa                                                               >  2:4528724/1‑149 (MQ=255)

                                                                                     tAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGa                                                            >  2:2572519/1‑149 (MQ=255)

                                                                                         tttCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAgtgt                                                        <  1:3815076/149‑1 (MQ=255)

                                                                                         tttCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAgtgt                                                        <  1:1985562/149‑1 (MQ=255)

                                                                                          ttCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTa                                                       <  1:129612/149‑1 (MQ=255)

                                                                                          ttCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTa                                                       >  2:3503294/1‑149 (MQ=255)

                                                                                            cGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAAc                                                     <  2:4140864/149‑1 (MQ=255)

                                                                                             gATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACt                                                    >  2:2277332/1‑149 (MQ=255)

                                                                                             gATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACt                                                    >  1:1472146/1‑149 (MQ=255)

                                                                                              aTGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTg                                                   >  1:3772552/1‑149 (MQ=255)

                                                                                                   gTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGcc                                              >  1:557044/1‑149 (MQ=255)

                                                                                                             tCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTc                                    >  1:3890599/1‑149 (MQ=255)

                                                                                                             tCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTc                                    <  1:884059/149‑1 (MQ=255)

                                                                                                               gcgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCCCCCACCAGGCTTCag                                  >  1:2657406/1‑149 (MQ=255)

                                                                                                                  gATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGc                               >  1:3292045/1‑149 (MQ=255)

                                                                                                                   aTGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCg                              >  1:506837/1‑149 (MQ=255)

                                                                                                                   aTGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCg                              >  2:3519210/1‑149 (MQ=255)

                                                                                                                     ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGtt                            >  1:2042593/1‑149 (MQ=255)

                                                                                                                          ccAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGa                       >  2:270576/1‑149 (MQ=255)

                                                                                                                           cAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGAt                      <  2:2441789/149‑1 (MQ=255)

                                                                                                                              aCCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCtt                   <  2:2341323/149‑1 (MQ=255)

                                                                                                                                cTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTcc                 >  2:1646053/1‑149 (MQ=255)

                                                                                                                                cTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTcc                 >  1:2715041/1‑149 (MQ=255)

                                                                                                                                 ttCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCa                <  1:3800596/149‑1 (MQ=255)

                                                                                                                                      gTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGa           >  2:680487/1‑149 (MQ=255)

                                                                                                                                        aCAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACg         <  1:1271493/149‑1 (MQ=255)

                                                                                                                                           gtgaagtgaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGc      >  2:3533298/1‑149 (MQ=255)

                                                                                                                                            tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt     <  1:1333769/149‑1 (MQ=255)

                                                                                                                                            tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt     >  1:350410/1‑149 (MQ=255)

                                                                                                                                            tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCAGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt     <  1:4343266/149‑1 (MQ=255)

                                                                                                                                             gaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTg    >  2:1308118/1‑149 (MQ=255)

                                                                                                                                               agttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCg  <  2:2111877/149‑1 (MQ=255)

GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCG  >  CP009273/693593‑693884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`