Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 710,187 A→T 90.1% S392C (AGT→TGT)  pgm → phosphoglucomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273710,1870AT90.1% 802.5 / 62.6 258S392C (AGT→TGT) pgmphosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base A (9/17);  new base T (112/120);  total (121/137)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

CGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTG  >  CP009273/710045‑710334
                                                                                                                                              |                                                                                                                                                   
 gTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAgg                                                                                                                                              >  1:4338532/1‑149 (MQ=255)
 gTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAgg                                                                                                                                              <  2:1318257/149‑1 (MQ=255)
     gTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGct                                                                                                                                          <  2:1935517/149‑1 (MQ=255)
     gTAAAACGCGGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGct                                                                                                                                          <  2:2367037/149‑1 (MQ=255)
      tAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGctt                                                                                                                                         >  2:1451207/1‑149 (MQ=255)
      tAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGctt                                                                                                                                         >  2:1596914/1‑149 (MQ=255)
           cGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGcttccttc                                                                                                                                    >  2:4025382/1‑149 (MQ=255)
               ggTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGc                                                                                                                                <  1:2865290/149‑1 (MQ=255)
                gTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCg                                                                                                                               >  2:2327974/1‑149 (MQ=255)
                  ttCATCTGCGATGATCGACCGTGTGGTAAACGACTTGGGCCGTAAACTGGTAGAAGTCCCTGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGtt                                                                                                                             <  1:2464087/149‑1 (MQ=255)
                   tCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGttt                                                                                                                            <  2:1805984/149‑1 (MQ=255)
                    cATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTc                                                                                                                           <  2:2042820/149‑1 (MQ=255)
                       cTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGAc                                                                                                                        <  2:614643/149‑1 (MQ=255)
                       cTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGAc                                                                                                                        <  2:4132511/149‑1 (MQ=255)
                        tGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACg                                                                                                                       >  1:2289046/1‑149 (MQ=255)
                        tGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACg                                                                                                                       <  2:4030649/149‑1 (MQ=255)
                         gCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACgg                                                                                                                      >  1:3064339/1‑149 (MQ=255)
                           gatgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCa                                                                                                                    <  2:2177747/149‑1 (MQ=255)
                           gatgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCa                                                                                                                    >  2:3392657/1‑149 (MQ=255)
                            atgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCAc                                                                                                                   >  2:3173109/1‑149 (MQ=255)
                            atgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCAc                                                                                                                   >  1:3914429/1‑149 (MQ=255)
                            atgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCAc                                                                                                                   >  2:4594154/1‑149 (MQ=255)
                             tgatCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACg                                                                                                                  >  1:1521328/1‑149 (MQ=255)
                               atCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGcc                                                                                                                >  2:1636487/1‑149 (MQ=255)
                                 cGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGt                                                                                                              <  1:3977216/149‑1 (MQ=255)
                                    ccGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGt                                                                                                           >  1:1193305/1‑149 (MQ=255)
                                     cGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTc                                                                                                          >  2:2526172/1‑149 (MQ=255)
                                      gtgtGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTcc                                                                                                         >  1:1090844/1‑149 (MQ=255)
                                      gtgtGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTcc                                                                                                         >  1:3873105/1‑149 (MQ=255)
                                      gtgtGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTcc                                                                                                         <  1:1913634/149‑1 (MQ=255)
                                          ggTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCg                                                                                                     >  2:563129/1‑149 (MQ=255)
                                          ggTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCg                                                                                                     >  1:4512716/1‑149 (MQ=255)
                                          ggTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCg                                                                                                     >  1:2114462/1‑149 (MQ=255)
                                           gTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGa                                                                                                    <  1:1004485/149‑1 (MQ=255)
                                           gTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGa                                                                                                    <  1:4782608/149‑1 (MQ=255)
                                               acgacgTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACaaa                                                                                                <  1:976654/149‑1 (MQ=255)
                                               aCGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACaaa                                                                                                >  1:1482733/1‑149 (MQ=255)
                                                   cTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACg                                                                                            >  2:638168/1‑149 (MQ=255)
                                                   cTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACg                                                                                            <  2:1124180/149‑1 (MQ=255)
                                                     tGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAGAGACGGc                                                                                          <  2:854773/149‑1 (MQ=255)
                                                         ccGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGcatca                                                                                      >  1:4138795/1‑149 (MQ=255)
                                                         ccGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGcatca                                                                                      <  1:858294/149‑1 (MQ=255)
                                                             aaaCTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGcatcatcat                                                                                  >  2:2526552/1‑149 (MQ=255)
                                                             aaaCTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGcatcatcat                                                                                  >  1:4328949/1‑149 (MQ=255)
                                                              aaCTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCAtg                                                                                 >  2:3962913/1‑149 (MQ=255)
                                                                 tGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATgtgt                                                                              >  1:1581173/1‑149 (MQ=255)
                                                                  ggTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTc                                                                             <  1:2713164/149‑1 (MQ=255)
                                                                   gTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTct                                                                            <  2:4359642/149‑1 (MQ=255)
                                                                     aGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTctgc                                                                          <  1:4493123/149‑1 (MQ=255)
                                                                     aGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTctgc                                                                          <  1:3696363/149‑1 (MQ=255)
                                                                      gcaGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTctgct                                                                         <  2:4081011/147‑1 (MQ=255)
                                                                       aaGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTctgctg                                                                        >  1:3617431/1‑149 (MQ=255)
                                                                       aaGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTctgctg                                                                        <  2:4404288/149‑1 (MQ=255)
                                                                           cccGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTggcgg                                                                    >  2:170884/1‑149 (MQ=255)
                                                                           cccGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTggcgg                                                                    >  2:4162056/1‑149 (MQ=255)
                                                                           cccGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTggcgg                                                                    >  1:1974776/1‑149 (MQ=255)
                                                                            ccGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTggcggc                                                                   >  2:1202769/1‑149 (MQ=255)
                                                                              ggTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGcggcgg                                                                 >  1:2620813/1‑149 (MQ=255)
                                                                               gTAGGTTTCAAATGGTTTGTCGATGGACTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGa                                                                <  1:881211/149‑1 (MQ=255)
                                                                                tAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTTGCGGCGAAGAGTGTGCAGGTGCTTCCTTCCTGCGTTTCTACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGATGGCTGCGGaa                                                               >  1:3475527/1‑149 (MQ=255)
                                                                                 aGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGaaa                                                              <  2:3444771/149‑1 (MQ=255)
                                                                                 aGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGaaa                                                              <  1:1631690/149‑1 (MQ=255)
                                                                                  ggTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAAt                                                             >  2:286163/1‑149 (MQ=255)
                                                                                     ttCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCAc                                                          <  1:3349814/149‑1 (MQ=255)
                                                                                      tCAACTGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCAcc                                                         <  2:4250249/149‑1 (MQ=255)
                                                                                         aaTGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCt                                                      >  2:3131660/1‑149 (MQ=255)
                                                                                         aaTGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCt                                                      <  2:2613237/149‑1 (MQ=255)
                                                                                         aaTGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCt                                                      <  2:4751249/149‑1 (MQ=255)
                                                                                               ttGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCAcc                                                >  1:2132759/1‑149 (MQ=255)
                                                                                                tGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCg                                               >  2:2943484/1‑149 (MQ=255)
                                                                                                   cGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTa                                            >  1:946733/1‑149 (MQ=255)
                                                                                                   cGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTa                                            >  1:1901377/1‑149 (MQ=255)
                                                                                                    gATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTaa                                           >  1:661740/1‑149 (MQ=255)
                                                                                                      tGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGa                                         >  1:1719455/1‑149 (MQ=255)
                                                                                                       ggTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGaa                                        <  2:3465451/149‑1 (MQ=255)
                                                                                                       ggTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGaa                                        >  1:2330216/1‑149 (MQ=255)
                                                                                                        gTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAAc                                       <  1:1318933/149‑1 (MQ=255)
                                                                                                         tCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAAcc                                      <  2:328235/149‑1 (MQ=255)
                                                                                                          cTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAAccc                                     >  2:3097133/1‑149 (MQ=255)
                                                                                                          cTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAAccc                                     <  1:2971446/149‑1 (MQ=255)
                                                                                                            gTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGc                                   >  1:2690367/1‑149 (MQ=255)
                                                                                                               cGACGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAgg                                >  2:969181/1‑149 (MQ=255)
                                                                                                                  cGGCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAac                             <  1:2172493/149‑1 (MQ=255)
                                                                                                                   ggCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAaca                            >  1:4020768/1‑149 (MQ=255)
                                                                                                                    gCAGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAacac                           >  2:754931/1‑149 (MQ=255)
                                                                                                                    gCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAacac                           <  2:137661/149‑1 (MQ=255)
                                                                                                                      aGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTa                         <  1:3132890/149‑1 (MQ=255)
                                                                                                                      aGCTTCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTa                         >  2:3313042/1‑149 (MQ=255)
                                                                                                                          tCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAAc                     <  1:2001996/149‑1 (MQ=255)
                                                                                                                          tCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAAc                     <  2:516849/149‑1 (MQ=255)
                                                                                                                          tCGGCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTAACCGGTAAGAACCCGCAGGAACACTACAAc                     >  2:3217672/1‑149 (MQ=255)
                                                                                                                            ggCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGa                   <  2:126601/149‑1 (MQ=255)
                                                                                                                            ggCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGa                   <  2:2946372/149‑1 (MQ=255)
                                                                                                                             gCTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGaa                  <  1:1391864/149‑1 (MQ=255)
                                                                                                                              cTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAAc                 <  1:1739599/149‑1 (MQ=255)
                                                                                                                              cTTTGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAAc                 <  2:1643506/149‑1 (MQ=255)
                                                                                                                               tttGGCGGCGAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACt                <  1:494139/149‑1 (MQ=255)
                                                                                                                                      gcgAAGAGTGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCaaaa         <  1:1699815/149‑1 (MQ=255)
                                                                                                                                           gagtgTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCtt    <  1:4751658/149‑1 (MQ=255)
                                                                                                                                             gagTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTg  >  2:3704815/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTG  >  CP009273/710045‑710334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑