Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 759,000 C→A 100% F177L (TTC→TTA)  sucC → succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273759,0000CA98.3% 1064.7 / 6.0 322F177L (TTC→TTA) sucCsuccinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (5/2);  new base A (166/149);  total (171/151)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 322 total aligned reads displayed.

GGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGCC  >  CP009273/758854‑759145
                                                                                                                                                  |                                                                                                                                                 
ggcggcGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAc                                                                                                                                                 >  1:1005579/1‑149 (MQ=255)
ggcggcGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAc                                                                                                                                                 <  1:1967828/149‑1 (MQ=255)
     cgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCaaaa                                                                                                                                            >  2:4010613/1‑149 (MQ=255)
     cgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCaaaa                                                                                                                                            <  1:180421/149‑1 (MQ=255)
      gTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCATATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAAt                                                                                                                                           >  2:2791859/1‑149 (MQ=255)
        ggAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                         <  2:226956/149‑1 (MQ=255)
        ggAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                         >  1:2436370/1‑149 (MQ=255)
        ggAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                         >  1:2068417/1‑149 (MQ=255)
         gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCtt                                                                                                                                        >  1:2977794/1‑149 (MQ=255)
         gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCtt                                                                                                                                        >  2:1093329/1‑149 (MQ=255)
         gAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCtt                                                                                                                                        >  1:2045140/1‑149 (MQ=255)
           aaTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCa                                                                                                                                      >  2:4651245/1‑149 (MQ=255)
            aTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCAt                                                                                                                                     >  2:3744929/1‑149 (MQ=255)
             tCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATg                                                                                                                                    >  1:3343356/1‑149 (MQ=255)
              cGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATgg                                                                                                                                   <  2:1617616/149‑1 (MQ=255)
               gAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATggg                                                                                                                                  <  1:1465857/149‑1 (MQ=255)
                 aaaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCTATGCCGTATCAGGTACGCGAGCTGGCTTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGcc                                                                                                                                <  2:4528065/149‑1 (MQ=255)
                  aaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCt                                                                                                                               <  1:932504/149‑1 (MQ=255)
                           gaagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAt                                                                                                                      <  2:2907489/149‑1 (MQ=255)
                           gaagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAt                                                                                                                      <  2:2186116/149‑1 (MQ=255)
                            aagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtt                                                                                                                     >  2:4686000/1‑149 (MQ=255)
                            aagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtt                                                                                                                     <  1:4279089/149‑1 (MQ=255)
                            aagaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtt                                                                                                                     <  1:4776524/149‑1 (MQ=255)
                             agaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAttt                                                                                                                    >  1:2304588/1‑149 (MQ=255)
                               aaaCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTc                                                                                                                  >  2:4137763/1‑149 (MQ=255)
                                aaCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTcc                                                                                                                 >  2:2501689/1‑149 (MQ=255)
                                 aCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCt                                                                                                                >  1:2547945/1‑149 (MQ=255)
                                  cTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTg                                                                                                               <  2:4302779/149‑1 (MQ=255)
                                       cACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAgcg                                                                                                          >  1:843568/1‑149 (MQ=255)
                                       cACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAgcg                                                                                                          >  1:3233605/1‑149 (MQ=255)
                                        aCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAgcgc                                                                                                         >  2:4734800/1‑149 (MQ=255)
                                         ccTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGcgcg                                                                                                        >  1:651060/1‑149 (MQ=255)
                                          cTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGa                                                                                                       >  2:3994612/1‑149 (MQ=255)
                                          cTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGa                                                                                                       <  1:572255/149‑1 (MQ=255)
                                          cTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGa                                                                                                       <  2:3703012/149‑1 (MQ=255)
                                           tGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAc                                                                                                      >  2:1164921/1‑149 (MQ=255)
                                           tGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAc                                                                                                      <  1:2384382/149‑1 (MQ=255)
                                            gATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                     <  2:1882280/149‑1 (MQ=255)
                                            gATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                     <  2:1512165/149‑1 (MQ=255)
                                                cATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGc                                                                                                 <  1:4604714/149‑1 (MQ=255)
                                                  tAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGt                                                                                               >  2:4254417/1‑149 (MQ=255)
                                                   aaaGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGtt                                                                                              <  2:527992/149‑1 (MQ=255)
                                                    aaGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTg                                                                                             >  1:267935/1‑149 (MQ=255)
                                                      gTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGAt                                                                                           <  2:1182330/149‑1 (MQ=255)
                                                               gATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCaa                                                                                  >  2:360682/1‑149 (MQ=255)
                                                                 tCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAcc                                                                                >  2:664840/1‑149 (MQ=255)
                                                                 gccGCTGCCTCGCCCGCTCCCGACGCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTGCAGCAGTTCACCACCCTCTGCCGGGGCCGGGCGACCATTGTCCTGGAGCGCGACCAGGCGTGGATCGAAAGCAAcc                                                                                <  2:4199322/148‑1 (MQ=255)
                                                                  ccGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAccc                                                                               <  2:533012/149‑1 (MQ=255)
                                                                  ccGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAccc                                                                               >  2:1732960/1‑149 (MQ=255)
                                                                     cTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCt                                                                            <  2:2687454/149‑1 (MQ=255)
                                                                       gACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTgg                                                                          >  1:4501263/1‑149 (MQ=255)
                                                                       gACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTgg                                                                          >  1:2808717/1‑149 (MQ=255)
                                                                        aCTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGt                                                                         <  1:2123622/149‑1 (MQ=255)
                                                                           ggCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGtcat                                                                      >  1:3635196/1‑149 (MQ=255)
                                                                                gATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCa                                                                 >  1:3855863/1‑149 (MQ=255)
                                                                                   gCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAAc                                                              >  1:2661868/1‑149 (MQ=255)
                                                                                    ccGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACa                                                             <  2:3108238/149‑1 (MQ=255)
                                                                                      gTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAgg                                                           >  2:501409/1‑149 (MQ=255)
                                                                                        aTCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGc                                                         >  1:754923/1‑149 (MQ=255)
                                                                                         tCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCg                                                        <  1:3755640/149‑1 (MQ=255)
                                                                                           aGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                      <  1:162557/149‑1 (MQ=255)
                                                                                           aGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                      >  1:894989/1‑149 (MQ=255)
                                                                                            gggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATc                                                     <  2:2745919/149‑1 (MQ=255)
                                                                                             ggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCt                                                    <  1:1317531/149‑1 (MQ=255)
                                                                                             ggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCt                                                    <  1:4113644/149‑1 (MQ=255)
                                                                                             ggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCt                                                    <  1:2626506/149‑1 (MQ=255)
                                                                                             ggACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCt                                                    <  1:2521324/149‑1 (MQ=255)
                                                                                                   gAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTg                                              >  1:3944337/1‑149 (MQ=255)
                                                                                                   gAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTg                                              >  1:151340/1‑149 (MQ=255)
                                                                                                     gCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGcc                                            <  2:1998318/149‑1 (MQ=255)
                                                                                                      cTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCAGATTTGCCt                                           <  1:4409392/149‑1 (MQ=255)
                                                                                                        ggCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAGCCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCg                                         >  2:3930897/1‑149 (MQ=255)
                                                                                                         gCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGa                                        >  2:4383594/1‑149 (MQ=255)
                                                                                                             tCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGc                                    >  2:631428/1‑149 (MQ=255)
                                                                                                               aaaCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCaa                                  >  2:812122/1‑149 (MQ=255)
                                                                                                                aaCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCaaa                                 <  2:773377/149‑1 (MQ=255)
                                                                                                                 aCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAAc                                <  1:1241050/149‑1 (MQ=255)
                                                                                                                    gggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTgg                             <  2:2031076/149‑1 (MQ=255)
                                                                                                                      gTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGgc                           >  2:1334228/1‑149 (MQ=255)
                                                                                                                           gAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGa                      <  2:3471771/149‑1 (MQ=255)
                                                                                                                           gAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGa                      <  2:2317175/149‑1 (MQ=255)
                                                                                                                               gTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGc                  <  1:4765345/149‑1 (MQ=255)
                                                                                                                               gTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGc                  <  1:2268320/149‑1 (MQ=255)
                                                                                                                                tAAACTGGTTCAGCAGTTAACCAAAATCTTCATGTGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATATGCCTCGACGGCAATCTGGGCGCTGACGGCa                 >  2:874619/1‑149 (MQ=255)
                                                                                                                                 aaaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTGTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCaa                <  1:4047024/149‑1 (MQ=255)
                                                                                                                                     tGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCa            >  1:873303/1‑149 (MQ=255)
                                                                                                                                     tGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCa            >  1:1220974/1‑149 (MQ=255)
                                                                                                                                      ggTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCAc           <  1:3059467/149‑1 (MQ=255)
                                                                                                                                         tCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGt        <  1:1272273/149‑1 (MQ=255)
                                                                                                                                           agcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTc      >  2:3905394/1‑149 (MQ=255)
                                                                                                                                           agcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTc      >  1:1100427/1‑149 (MQ=255)
                                                                                                                                            gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc     <  2:3343356/149‑1 (MQ=255)
                                                                                                                                            gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc     >  2:2108763/1‑149 (MQ=255)
                                                                                                                                            gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc     <  1:333180/149‑1 (MQ=255)
                                                                                                                                            gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc     <  1:1416482/149‑1 (MQ=255)
                                                                                                                                            gcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc     <  2:781414/149‑1 (MQ=255)
                                                                                                                                              agTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGc   >  2:1818270/1‑149 (MQ=255)
                                                                                                                                              agTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGc   >  1:4351625/1‑149 (MQ=255)
                                                                                                                                               gTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGcc  <  2:2377881/149‑1 (MQ=255)
                                                                                                                                               gTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGcc  >  1:1101931/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGCC  >  CP009273/758854‑759145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑