Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 788,902 G→T 100% R115S (CGT→AGT)  modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273788,9020GT99.7% 976.8 / ‑4.5 283R115S (CGT→AGT) modFfused molybdate transporter subunits of ABC superfamily: ATP‑binding components
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (147/135);  total (147/136)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

GAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAGTGATG  >  CP009273/788756‑789047
                                                                                                                                                  |                                                                                                                                                 
gAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAActcg                                                                                                                                                 >  2:1039060/1‑149 (MQ=255)
 aaCGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCgg                                                                                                                                                >  1:3065024/1‑149 (MQ=255)
 aaCGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCgg                                                                                                                                                >  2:2748324/1‑149 (MQ=255)
  aCGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGt                                                                                                                                               <  1:2185920/149‑1 (MQ=255)
   cGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTg                                                                                                                                              <  2:322462/149‑1 (MQ=255)
   cGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTg                                                                                                                                              >  1:1904322/1‑149 (MQ=255)
   cGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTg                                                                                                                                              <  1:4388681/149‑1 (MQ=255)
       tcatcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATc                                                                                                                                          >  1:2048670/1‑149 (MQ=255)
          tcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCtt                                                                                                                                       >  1:1307754/1‑149 (MQ=255)
          tcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCtt                                                                                                                                       <  1:1359526/149‑1 (MQ=255)
          tcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCtt                                                                                                                                       <  1:332507/149‑1 (MQ=255)
          tcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCtt                                                                                                                                       >  1:4106647/1‑149 (MQ=255)
           caAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCttt                                                                                                                                      <  1:110918/149‑1 (MQ=255)
           caAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCttt                                                                                                                                      >  1:3152145/1‑149 (MQ=255)
           caAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCttt                                                                                                                                      >  1:760631/1‑149 (MQ=255)
             aGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTAc                                                                                                                                    <  1:550130/149‑1 (MQ=255)
               aaTCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACtt                                                                                                                                  <  1:1933751/149‑1 (MQ=255)
                aTCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTc                                                                                                                                 >  1:3748924/1‑149 (MQ=255)
                 tCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCa                                                                                                                                >  2:2986310/1‑149 (MQ=255)
                   aacaaGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATc                                                                                                                              >  1:2496831/1‑149 (MQ=255)
                   aacaaGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATc                                                                                                                              <  1:1024812/149‑1 (MQ=255)
                    acaaGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATcc                                                                                                                             <  1:2601814/149‑1 (MQ=255)
                     caaGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTGTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCt                                                                                                                            >  2:934490/1‑149 (MQ=255)
                       aGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGa                                                                                                                          >  2:2444700/1‑149 (MQ=255)
                        gTCAGCCTCCGACATCTGCGACTGAGACAGCAGTGATTTTCTCGTCTCGCCTTTGTATATTTATATAAAGGCTCCGTCGACGAGGTCTTTAATAAGGTAATTCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGaa                                                                                                                         <  2:3491435/149‑1 (MQ=255)
                          cAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATg                                                                                                                       <  1:2656341/149‑1 (MQ=255)
                             gCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGAtc                                                                                                                    <  2:2184086/149‑1 (MQ=255)
                               tCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGAtctc                                                                                                                  >  1:2971666/1‑149 (MQ=255)
                                  gACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGc                                                                                                               >  2:2730623/1‑149 (MQ=255)
                                  gACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGc                                                                                                               >  2:720956/1‑149 (MQ=255)
                                  gACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGc                                                                                                               >  2:1490556/1‑149 (MQ=255)
                                   aCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGcc                                                                                                              >  2:2070438/1‑149 (MQ=255)
                                       cAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGtcg                                                                                                          >  2:4208690/1‑147 (MQ=255)
                                       cAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAg                                                                                                          >  2:157854/1‑149 (MQ=255)
                                          cgcCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGc                                                                                                       <  1:1763213/149‑1 (MQ=255)
                                                     gcagGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCa                                                                                            >  2:4737259/1‑149 (MQ=255)
                                                     gcagGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCa                                                                                            >  2:825654/1‑149 (MQ=255)
                                                        gggTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCt                                                                                         >  2:1555986/1‑149 (MQ=255)
                                                        gggTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCt                                                                                         >  2:379771/1‑149 (MQ=255)
                                                        gggTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCt                                                                                         >  1:653743/1‑149 (MQ=255)
                                                           tttttCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCg                                                                                      >  2:4330503/1‑149 (MQ=255)
                                                             tttCGCGTCTCGCCAGTGGACAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGcc                                                                                    <  2:1403018/149‑1 (MQ=255)
                                                               tCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAg                                                                                  <  2:1646039/149‑1 (MQ=255)
                                                                  cgTCTCGCCTGTGGTAAGGTATTGACAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGGTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCTGCCGTTGTGCGTCCGGTGTCATCTTCGCCAGGGc                                                                               <  2:4357848/149‑1 (MQ=255)
                                                                   gTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGcc                                                                              >  1:2249914/1‑149 (MQ=255)
                                                                    tctcGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCg                                                                             >  1:423076/1‑149 (MQ=255)
                                                                     ctcGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGa                                                                            <  1:1624745/149‑1 (MQ=255)
                                                                     ctcGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGa                                                                            >  1:1818300/1‑149 (MQ=255)
                                                                      tcGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAg                                                                           <  1:496288/149‑1 (MQ=255)
                                                                       cGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGc                                                                          >  1:1401263/1‑149 (MQ=255)
                                                                        gCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCa                                                                         >  2:2552934/1‑149 (MQ=255)
                                                                        gCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCa                                                                         <  2:2355863/149‑1 (MQ=255)
                                                                              ggAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCgg                                                                   >  1:4658795/1‑149 (MQ=255)
                                                                                aaaGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGgtg                                                                 <  1:3028383/149‑1 (MQ=255)
                                                                                    gTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTAt                                                             >  2:2991847/1‑149 (MQ=255)
                                                                                    gTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTAt                                                             >  2:1736586/1‑149 (MQ=255)
                                                                                    gTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTAt                                                             >  1:4732786/1‑149 (MQ=255)
                                                                                      aTTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTc                                                           >  2:3348831/1‑149 (MQ=255)
                                                                                           aaGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTg                                                      >  1:216991/1‑149 (MQ=255)
                                                                                             gCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCAGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGcc                                                    <  2:4769205/149‑1 (MQ=255)
                                                                                               gtcggtcgAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCAt                                                  >  2:3043749/1‑149 (MQ=255)
                                                                                                 cggtcgAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATtc                                                >  2:2112608/1‑149 (MQ=255)
                                                                                                 cggtcgAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATtc                                                >  1:3455563/1‑149 (MQ=255)
                                                                                                   gtcgAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATtcgt                                              <  2:867809/149‑1 (MQ=255)
                                                                                                     cgAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATtcgtcg                                            <  2:4516702/149‑1 (MQ=255)
                                                                                                      gaggagGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGc                                           >  1:2592823/1‑149 (MQ=255)
                                                                                                      gaggagGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGc                                           <  1:3766375/149‑1 (MQ=255)
                                                                                                       aggaggGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCt                                          >  2:190480/1‑149 (MQ=255)
                                                                                                       aggaggGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCt                                          >  2:2006965/1‑149 (MQ=255)
                                                                                                       aggaggGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCt                                          >  1:3942794/1‑149 (MQ=255)
                                                                                                        ggaggGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTg                                         >  2:2853393/1‑149 (MQ=255)
                                                                                                         gaggGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGa                                        <  2:342232/149‑1 (MQ=255)
                                                                                                               ggTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGtt                                  >  1:2556932/1‑149 (MQ=255)
                                                                                                               ggTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGtt                                  <  1:3523581/149‑1 (MQ=255)
                                                                                                                gTAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGttt                                 >  2:516933/1‑149 (MQ=255)
                                                                                                                 tAATACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGtttt                                <  1:1205759/149‑1 (MQ=255)
                                                                                                                    tACCGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGc                             >  2:3905572/1‑149 (MQ=255)
                                                                                                                      ccGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATCTCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTGTTGCaa                           >  2:1234267/1‑149 (MQ=255)
                                                                                                                      ccGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCaa                           <  2:2483107/149‑1 (MQ=255)
                                                                                                                      ccGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCaa                           <  2:649277/149‑1 (MQ=255)
                                                                                                                       cGAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAAt                          <  1:452013/149‑1 (MQ=255)
                                                                                                                        gAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAAtt                         >  1:2584629/1‑149 (MQ=255)
                                                                                                                        gAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAAtt                         <  1:4010083/149‑1 (MQ=255)
                                                                                                                        gAACTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAAtt                         <  1:1895871/149‑1 (MQ=255)
                                                                                                                          aCTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGc                       <  2:4122562/149‑1 (MQ=255)
                                                                                                                          aCTGCTGCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGc                       >  1:845705/1‑149 (MQ=255)
                                                                                                                           ctgctgCGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCt                      >  1:3401378/1‑149 (MQ=255)
                                                                                                                            tgctgcGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTc                     <  2:3131437/149‑1 (MQ=255)
                                                                                                                             gctgcGCCAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCg                    >  2:1374871/1‑149 (MQ=255)
                                                                                                                                   ccAGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGgag              <  1:1569085/149‑1 (MQ=255)
                                                                                                                                     aGTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGgagag            <  2:2639053/149‑1 (MQ=255)
                                                                                                                                      gTTGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGgagaga           >  1:380764/1‑149 (MQ=255)
                                                                                                                                       ttGCATGCAACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGAc          <  1:988818/149‑1 (MQ=255)
                                                                                                                                        tgcatgcaACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACg         <  2:73895/149‑1 (MQ=255)
                                                                                                                                        tgcatgcaACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACg         >  1:2781975/1‑149 (MQ=255)
                                                                                                                                          catgcaACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAg       <  1:2305670/149‑1 (MQ=255)
                                                                                                                                          catgcaACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAg       <  1:3430687/149‑1 (MQ=255)
                                                                                                                                             gcaACTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAGTGa    <  2:184108/149‑1 (MQ=255)
                                                                                                                                               aaCTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAGTGAtg  <  2:3598636/149‑1 (MQ=255)
                                                                                                                                               aaCTCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAAGTGCTCGAAGGAGAGACGAGTGAtg  <  2:653743/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTTTTTGCAATTGCTCGAAGGAGAGACGAGTGATG  >  CP009273/788756‑789047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑