Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 824,171 A→C 26.3% F89L (TTT→TTG)  ybhF ← putative transporter subunit of ABC superfamily: ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273824,1710AC26.3% 399.1 / 251.0 285F89L (TTT→TTG) ybhFputative transporter subunit of ABC superfamily: ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base A (114/96);  new base C (32/43);  total (146/139)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 290 total aligned reads displayed.

CTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGC  >  CP009273/824024‑824318
                                                                                                                                                   |                                                                                                                                                   
cTTGCCCGCCAGGGGTCCGGTAAACGGCCCAAGTGTCGTAAACTCCAGCAGGCGAGCAATAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCaa                                                                                                                                                    <  1:917247/149‑1 (MQ=255)
 ttGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCcaa                                                                                                                                                   <  2:2456511/149‑1 (MQ=255)
 ttGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCaaa                                                                                                                                                   >  1:33153/1‑149 (MQ=255)
   gCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAAtt                                                                                                                                                 <  2:4563860/149‑1 (MQ=255)
     ccGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTc                                                                                                                                               >  1:82924/1‑149 (MQ=255)
     ccGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTc                                                                                                                                               <  1:579381/149‑1 (MQ=255)
         cAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCg                                                                                                                                           >  2:3878779/1‑149 (MQ=255)
           ggCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGc                                                                                                                                         <  1:1867034/149‑1 (MQ=255)
             cGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCat                                                                                                                                       <  2:2931279/149‑1 (MQ=255)
              gTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCata                                                                                                                                      <  1:1102915/149‑1 (MQ=255)
              gTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCata                                                                                                                                      <  2:231045/149‑1 (MQ=255)
              gTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCata                                                                                                                                      <  1:782254/149‑1 (MQ=255)
               tCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCatat                                                                                                                                     >  1:3194022/1‑149 (MQ=255)
               tCCGGTAAACGGCCCAAGAGACGTAAACTCAAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGAGGCatat                                                                                                                                     >  2:1119053/1‑149 (MQ=255)
                 cGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATaa                                                                                                                                   <  1:4593005/149‑1 (MQ=255)
                 cGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATaa                                                                                                                                   >  1:2287720/1‑149 (MQ=255)
                  ggTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAACTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAAc                                                                                                                                  <  2:1604291/149‑1 (MQ=255)
                  ggTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAAc                                                                                                                                  <  1:1636113/149‑1 (MQ=255)
                      aaCGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAg                                                                                                                              >  1:4371423/1‑149 (MQ=255)
                      aaCGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAg                                                                                                                              >  1:2315864/1‑149 (MQ=255)
                         ggCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCAc                                                                                                                           <  1:3239755/149‑1 (MQ=255)
                              aaGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGt                                                                                                                      <  1:2400942/149‑1 (MQ=255)
                                gagaCGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGc                                                                                                                    <  2:1358678/149‑1 (MQ=255)
                                gagaCGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGc                                                                                                                    <  2:1499883/149‑1 (MQ=255)
                                     gTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAgcgc                                                                                                               >  1:4225887/1‑149 (MQ=255)
                                      tAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAgcgcg                                                                                                              >  1:1801195/1‑149 (MQ=255)
                                       aaaCTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAgcgcgc                                                                                                             <  2:464092/149‑1 (MQ=255)
                                       aaaCTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAgcgcgc                                                                                                             >  2:1222980/1‑149 (MQ=255)
                                       aaaCTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAgcgcgc                                                                                                             <  2:2133862/149‑1 (MQ=255)
                                        aaCTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGcc                                                                                                            <  1:3957599/149‑1 (MQ=255)
                                        aaCTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGcc                                                                                                            >  2:2685345/1‑149 (MQ=255)
                                               gcagGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGttt                                                                                                     >  1:3568015/1‑149 (MQ=255)
                                                  ggCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg                                                                                                  >  1:1385651/1‑149 (MQ=255)
                                                  ggCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTg                                                                                                  >  1:3053481/1‑149 (MQ=255)
                                                   gCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGa                                                                                                 <  1:4053890/149‑1 (MQ=255)
                                                     gAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATc                                                                                               >  2:3117196/1‑149 (MQ=255)
                                                     gAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATc                                                                                               >  1:3529830/1‑149 (MQ=255)
                                                      aGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg                                                                                              >  2:4120762/1‑149 (MQ=255)
                                                       gCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAACTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg                                                                                             <  2:3521659/149‑1 (MQ=255)
                                                       gCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCgg                                                                                             <  1:2695446/149‑1 (MQ=255)
                                                        cAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGa                                                                                            >  2:2602439/1‑149 (MQ=255)
                                                         aaaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGAt                                                                                           >  2:686125/1‑149 (MQ=255)
                                                            aGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAAGTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaa                                                                                        >  1:3121515/1‑149 (MQ=255)
                                                             gTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCaaa                                                                                       >  1:1882639/1‑149 (MQ=255)
                                                                tGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGcc                                                                                    <  2:3845634/149‑1 (MQ=255)
                                                                  cTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCaa                                                                                  <  2:3232340/149‑1 (MQ=255)
                                                                   ttACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTGTTTGATCGGATCAAAGCCAAt                                                                                 >  1:51472/1‑149 (MQ=255)
                                                                     aCGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCa                                                                               <  2:2645712/149‑1 (MQ=255)
                                                                     aCGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAATCCAATCa                                                                               >  1:2593437/1‑149 (MQ=255)
                                                                     aCGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCa                                                                               <  2:249759/149‑1 (MQ=255)
                                                                     aCGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCa                                                                               >  1:2854442/1‑149 (MQ=255)
                                                                       gTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCAcc                                                                             >  1:642334/1‑149 (MQ=255)
                                                                          ccTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTg                                                                          <  1:1281467/149‑1 (MQ=255)
                                                                          ccTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTg                                                                          <  1:4759170/149‑1 (MQ=255)
                                                                                 ggTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGc                                                                   <  1:1967973/149‑1 (MQ=255)
                                                                                  gTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGcc                                                                  <  1:1027899/149‑1 (MQ=255)
                                                                                    gACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGc                                                                <  1:190626/149‑1 (MQ=255)
                                                                                    gACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGc                                                                <  1:2781672/149‑1 (MQ=255)
                                                                                          gcgcAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGCTCAAAGCCAATCACCGGGGCACAGCCGCGGTCgg                                                          >  2:4395876/1‑149 (MQ=255)
                                                                                             cAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGtt                                                       <  2:3988909/149‑1 (MQ=255)
                                                                                              aaaTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGttt                                                      <  2:638018/149‑1 (MQ=255)
                                                                                               aaTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTGTTTTATAGGATCAAAGCCCAACACCGAGGCCCTGCCGCCGTCGGGgttg                                                     >  1:3644018/1‑148 (MQ=255)
                                                                                               aaTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTc                                                     <  2:1024508/149‑1 (MQ=255)
                                                                                                aTCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCa                                                    >  1:1442275/1‑149 (MQ=255)
                                                                                                 tCCGCGGACAGTTTGTGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAg                                                   <  2:3154774/149‑1 (MQ=255)
                                                                                                   cgcgTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTa                                                 <  1:4115664/149‑1 (MQ=255)
                                                                                                   cgcgTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTa                                                 <  1:2905188/149‑1 (MQ=255)
                                                                                                     cgTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAAt                                               <  2:3327965/149‑1 (MQ=255)
                                                                                                      gTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCCAAGCCAATCCCCGTGGCACTGCCGCTGTCGGGTTTCAGTAATc                                              >  2:2995854/1‑149 (MQ=255)
                                                                                                       tACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCACTCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATcc                                             <  2:480095/149‑1 (MQ=255)
                                                                                                        aCAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATccc                                            <  1:2048036/149‑1 (MQ=255)
                                                                                                          aGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc                                          <  1:4380692/149‑1 (MQ=255)
                                                                                                          aGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGc                                          <  1:1599119/149‑1 (MQ=255)
                                                                                                          aGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCCTGGCACTGCCGCTGTCGGGTTTAAGTAATACCGc                                          >  2:4081877/1‑149 (MQ=255)
                                                                                                           gATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGTTAGGTTCAAATCCAAACACCCTGGGCCTGCCGCTGTGGGGGTTGAGTAATACCGcc                                         >  1:1649770/1‑149 (MQ=255)
                                                                                                           gATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGcc                                         <  1:3780420/149‑1 (MQ=255)
                                                                                                           gATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGcc                                         <  1:365193/149‑1 (MQ=255)
                                                                                                             ttGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTTTTAGGATCAAATCCAAACACCCTGGCACAGCCGCTGTCGGGTTTAAGTAATACCCCCaa                                       >  1:148041/1‑149 (MQ=255)
                                                                                                               gAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACa                                     <  1:3844154/149‑1 (MQ=255)
                                                                                                               gAGGTTCTCCATCACCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACa                                     <  1:2332666/149‑1 (MQ=255)
                                                                                                               gAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACa                                     >  2:4778332/1‑149 (MQ=255)
                                                                                                                       ccATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc                             <  2:224230/149‑1 (MQ=255)
                                                                                                                       ccATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATc                             <  2:659097/149‑1 (MQ=255)
                                                                                                                          tCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGc                          >  2:2669214/1‑149 (MQ=255)
                                                                                                                            aCCGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACAACCGCATCAGCGt                        >  2:1831327/1‑149 (MQ=255)
                                                                                                                             ccGTGAGATCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTTATCGGATCAAAGCCAATAACCGTGGCACAGCCGCTGTCGGGTTTGAGTAAAACCGCCAACAACCGCCACAGCGTg                       >  1:1308284/1‑149 (MQ=255)
                                                                                                                             ccGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTg                       <  1:2501047/149‑1 (MQ=255)
                                                                                                                             ccGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATACCGCCAACATACGCATCAGCGTg                       >  1:4308259/1‑149 (MQ=255)
                                                                                                                               gTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAATCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATACCGCCAACATCCGCATCAGCGTGGt                     >  1:371282/1‑149 (MQ=255)
                                                                                                                                tGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtt                    <  2:1386105/149‑1 (MQ=255)
                                                                                                                                tGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtt                    <  2:4620792/149‑1 (MQ=255)
                                                                                                                                tGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtt                    <  2:2222998/149‑1 (MQ=255)
                                                                                                                                  agaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGtttt                  <  2:3629406/149‑1 (MQ=255)
                                                                                                                                    aTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATAGGATCAAAGCCAATAACCGTGGCACTGCCGCTGTCGGGTTTCAGTAAACCCGCCCACATCCGCAACAGCGTGGTTTTAc                >  2:832725/1‑149 (MQ=255)
                                                                                                                                     tCTTCATACAGACCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcc               <  2:2385248/149‑1 (MQ=255)
                                                                                                                                     tCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACAGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcc               >  2:4528243/1‑149 (MQ=255)
                                                                                                                                             cAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCGGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGt       >  2:2838862/1‑149 (MQ=255)
                                                                                                                                              aGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGTTCGGATCAAAGCCAAACACCGTGGCACAGCCGCTGTCGGGGTTCAGTAAACCCGCCCACATCCGCAACAGCGTGGTTTTACCTGCACCGTc      >  1:234678/1‑149 (MQ=255)
                                                                                                                                                aCCCAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTGTTTTTTAGGATCAAAGCCAAACACCCTGGCACAGCCGCAGTGGGGGTTCAGTAAACACCCCAACAAACCCCACAGCGGGGGTTTTCCAGCACCCTCCg    >  1:1675472/1‑149 (MQ=255)
                                                                                                                                                 ccAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCCACATACGCATAAGCGTGGTTTTAACTGCACCGTCCgg   >  2:1754360/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGC  >  CP009273/824024‑824318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑