Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 81,562 C→A 100% I236I (ATC→ATA)  leuO → global transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927381,5620CA99.7% 1219.1 / ‑4.3 353I236I (ATC→ATA) leuOglobal transcription factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (174/178);  total (175/178)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 360 total aligned reads displayed.

CTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCA  >  CP009273/81416‑81707
                                                                                                                                                  |                                                                                                                                                 
 tGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATagcg                                                                                                                                                >  2:3281062/1‑149 (MQ=255)
  ggTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGt                                                                                                                                               <  2:386091/149‑1 (MQ=255)
    tAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTAt                                                                                                                                             <  2:4053102/149‑1 (MQ=255)
    tAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTAt                                                                                                                                             <  2:304601/149‑1 (MQ=255)
    tAGCCAGAAAAAATCATCCAACACTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTAt                                                                                                                                             <  2:2449890/149‑1 (MQ=255)
       ccAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAg                                                                                                                                          <  2:1171709/149‑1 (MQ=255)
       ccAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAg                                                                                                                                          <  2:910820/149‑1 (MQ=255)
         aGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAggg                                                                                                                                        <  1:1888881/149‑1 (MQ=255)
         aGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAggg                                                                                                                                        >  2:1953886/1‑149 (MQ=255)
          gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGc                                                                                                                                       >  1:769730/1‑149 (MQ=255)
          gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGc                                                                                                                                       <  2:4149554/149‑1 (MQ=255)
          gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGc                                                                                                                                       >  1:3376909/1‑149 (MQ=255)
          gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGc                                                                                                                                       >  2:3923187/1‑149 (MQ=255)
            aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCAt                                                                                                                                     <  2:248625/149‑1 (MQ=255)
            aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCAt                                                                                                                                     <  2:2810447/149‑1 (MQ=255)
               aaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGc                                                                                                                                  >  1:1732069/1‑149 (MQ=255)
                atcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCa                                                                                                                                 >  2:4366563/1‑149 (MQ=255)
                atcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCa                                                                                                                                 >  1:352887/1‑149 (MQ=255)
                 tcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCaa                                                                                                                                >  2:1358268/1‑149 (MQ=255)
                   atcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatg                                                                                                                              >  1:4364331/1‑149 (MQ=255)
                       aacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAAtgatga                                                                                                                          <  1:421418/149‑1 (MQ=255)
                        acaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATGAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAg                                                                                                                         <  2:3796177/149‑1 (MQ=255)
                           aTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGt                                                                                                                      <  2:1576063/149‑1 (MQ=255)
                           aTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGt                                                                                                                      <  1:2600940/149‑1 (MQ=255)
                             tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTAc                                                                                                                    >  2:4331616/1‑149 (MQ=255)
                              aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                   <  2:1055158/149‑1 (MQ=255)
                              aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                   <  2:2348225/149‑1 (MQ=255)
                              aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                   <  1:2007907/149‑1 (MQ=255)
                              aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                   <  1:3851586/149‑1 (MQ=255)
                              aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                   <  1:2875386/149‑1 (MQ=255)
                                    ccGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgt                                                                                                             >  1:2477589/1‑149 (MQ=255)
                                    ccGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGGTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgt                                                                                                             >  1:411438/1‑149 (MQ=255)
                                         aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGgtgt                                                                                                        <  1:3298210/149‑1 (MQ=255)
                                          cTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATTGTTTCGCGTCATTTAGTCAACCTTGCTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACATAGCGTGGTGTc                                                                                                       >  1:1569186/1‑149 (MQ=255)
                                            gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                     >  1:1014675/1‑149 (MQ=255)
                                            gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                     <  1:4777760/149‑1 (MQ=255)
                                             aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCa                                                                                                    <  1:152793/149‑1 (MQ=255)
                                              aaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCCTGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaa                                                                                                   >  2:1996984/1‑149 (MQ=255)
                                               aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaaa                                                                                                  <  2:2983814/149‑1 (MQ=255)
                                                cATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAAc                                                                                                 >  2:4618800/1‑149 (MQ=255)
                                                    atgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAt                                                                                             <  2:1114397/149‑1 (MQ=255)
                                                     tgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAtt                                                                                            <  2:3513524/149‑1 (MQ=255)
                                                            aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTCTAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                                                     >  2:3651348/1‑149 (MQ=255)
                                                            aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                                                     >  1:3252456/1‑149 (MQ=255)
                                                            aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                                                     >  2:3652437/1‑149 (MQ=255)
                                                             aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCt                                                                                    >  2:4565757/1‑149 (MQ=255)
                                                              cGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTa                                                                                   >  2:2034190/1‑149 (MQ=255)
                                                              cGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTa                                                                                   <  1:115485/149‑1 (MQ=255)
                                                               gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAt                                                                                  <  2:812013/149‑1 (MQ=255)
                                                               gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAt                                                                                  >  1:2896742/1‑149 (MQ=255)
                                                               gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAt                                                                                  <  2:2275472/149‑1 (MQ=255)
                                                                aacaacATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAtt                                                                                 >  1:3720398/1‑149 (MQ=255)
                                                                 acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCCAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                                                >  2:3297318/1‑149 (MQ=255)
                                                                            cggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTAAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtgg                                                                     >  1:31220/1‑149 (MQ=255)
                                                                                 tCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctggc                                                                <  2:1443824/149‑1 (MQ=255)
                                                                                 tCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctggc                                                                <  2:3388061/149‑1 (MQ=255)
                                                                                   gCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGgctggctg                                                              >  2:2935760/1‑149 (MQ=255)
                                                                                   gCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGgctggctg                                                              >  1:3621513/1‑149 (MQ=255)
                                                                                     tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                            >  2:1561933/1‑149 (MQ=255)
                                                                                     tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                            >  1:798244/1‑149 (MQ=255)
                                                                                     tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                            <  2:2672993/149‑1 (MQ=255)
                                                                                       gATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAaga                                                          >  2:3750341/1‑149 (MQ=255)
                                                                                       gATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAaga                                                          <  1:4459562/149‑1 (MQ=255)
                                                                                          cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAATTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGtt                                                       <  2:2064828/149‑1 (MQ=255)
                                                                                            tttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCg                                                     >  2:792096/1‑149 (MQ=255)
                                                                                             ttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGc                                                    <  1:3804333/149‑1 (MQ=255)
                                                                                                   tCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                                              <  2:4448363/149‑1 (MQ=255)
                                                                                                   tCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                                              >  2:1718677/1‑149 (MQ=255)
                                                                                                    cATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATcc                                             <  1:2802826/149‑1 (MQ=255)
                                                                                                    cATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATcc                                             <  2:3532477/149‑1 (MQ=255)
                                                                                                     aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                            <  1:71940/149‑1 (MQ=255)
                                                                                                     aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                            <  2:1264351/149‑1 (MQ=255)
                                                                                                     aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                            <  2:1432076/149‑1 (MQ=255)
                                                                                                      tttAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCtt                                           <  1:972071/149‑1 (MQ=255)
                                                                                                       ttAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTa                                          <  1:630833/149‑1 (MQ=255)
                                                                                                        tAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAg                                         >  2:3162968/1‑149 (MQ=255)
                                                                                                          gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                       >  2:5235/1‑149 (MQ=255)
                                                                                                          gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                       >  1:2696439/1‑149 (MQ=255)
                                                                                                            cAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAtt                                     <  2:424967/149‑1 (MQ=255)
                                                                                                            cAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAtt                                     <  2:3956379/149‑1 (MQ=255)
                                                                                                             aaCCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTa                                    >  2:1669464/1‑149 (MQ=255)
                                                                                                              aCCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTAc                                   >  2:2564192/1‑149 (MQ=255)
                                                                                                               ccTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa                                  >  1:1430972/1‑149 (MQ=255)
                                                                                                               ccTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa                                  <  1:474350/149‑1 (MQ=255)
                                                                                                                    gTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTAt                             >  1:1341258/1‑149 (MQ=255)
                                                                                                                       tGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAc                          >  2:4789122/1‑149 (MQ=255)
                                                                                                                        gACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAcc                         >  2:1825176/1‑149 (MQ=255)
                                                                                                                          cacGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGc                       <  1:2159088/149‑1 (MQ=255)
                                                                                                                                aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCTATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                 <  1:2739203/149‑1 (MQ=255)
                                                                                                                                aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                 >  2:4691537/1‑149 (MQ=255)
                                                                                                                                 gATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                <  1:2942386/149‑1 (MQ=255)
                                                                                                                                   tAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaa              <  2:71730/149‑1 (MQ=255)
                                                                                                                                   tAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaa              <  2:2137000/149‑1 (MQ=255)
                                                                                                                                        aagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaa         <  1:4618800/149‑1 (MQ=255)
                                                                                                                                         agcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaa        <  1:4032477/149‑1 (MQ=255)
                                                                                                                                          gcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaa       >  1:3592639/1‑149 (MQ=255)
                                                                                                                                          gcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTGAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaa       >  2:3106980/1‑149 (MQ=255)
                                                                                                                                           ccAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaac      >  1:3277609/1‑149 (MQ=255)
                                                                                                                                             aGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcag    <  2:597638/149‑1 (MQ=255)
                                                                                                                                               tataGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagca  >  1:3043286/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCA  >  CP009273/81416‑81707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑