Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 850,161 G→T 32.1% P353P (CCG→CCT)  ybiR → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273850,1610GT32.1% 502.3 / 206.6 290P353P (CCG→CCT) ybiRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (94/101);  new base T (45/50);  total (139/151)
Fisher's exact test for biased strand distribution p-value = 9.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

TGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCG  >  CP009273/850013‑850309
                                                                                                                                                    |                                                                                                                                                    
tGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGACCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCCATACCg                                                                                                                                                      >  1:717817/1‑149 (MQ=255)
 gAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGa                                                                                                                                                     >  1:3825175/1‑149 (MQ=255)
  aaCTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTAt                                                                                                                                                    >  1:3172787/1‑149 (MQ=255)
    cTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGc                                                                                                                                                  >  2:4355525/1‑149 (MQ=255)
     tATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATATCTATTCAATAACGATGCt                                                                                                                                                 >  1:1793694/1‑149 (MQ=255)
       tgtgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCtgt                                                                                                                                               >  1:3459139/1‑149 (MQ=255)
        gtgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgtt                                                                                                                                              <  2:4254276/149‑1 (MQ=255)
          gccgccGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgttgt                                                                                                                                            <  1:3288555/149‑1 (MQ=255)
          gccgccGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCtgttgt                                                                                                                                            >  1:2585333/1‑149 (MQ=255)
          gccgccGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCtgttgt                                                                                                                                            >  2:4050283/1‑149 (MQ=255)
          gccgccGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCtgttgt                                                                                                                                            >  2:4106554/1‑149 (MQ=255)
           ccgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTtgtg                                                                                                                                           <  1:3038838/149‑1 (MQ=255)
           ccgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTtgtg                                                                                                                                           <  2:236260/149‑1 (MQ=255)
            cgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTgg                                                                                                                                          >  2:4378057/1‑149 (MQ=255)
              ccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggc                                                                                                                                        >  1:3332835/1‑149 (MQ=255)
              ccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGggc                                                                                                                                        <  2:4578110/149‑1 (MQ=255)
               cgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATTCTGTTGTGggcg                                                                                                                                       >  1:1826275/1‑149 (MQ=255)
                    ttattaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGtt                                                                                                                                  >  2:3654663/1‑149 (MQ=255)
                      attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTtgg                                                                                                                                >  1:3912952/1‑149 (MQ=255)
                       ttaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTtggt                                                                                                                               >  2:2590538/1‑149 (MQ=255)
                       ttaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTtggt                                                                                                                               >  1:1970430/1‑149 (MQ=255)
                           tGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGa                                                                                                                           <  2:2112878/149‑1 (MQ=255)
                             gTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                         >  1:4714361/1‑149 (MQ=255)
                              tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGatat                                                                                                                        <  2:1673020/149‑1 (MQ=255)
                              tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGatat                                                                                                                        >  1:2808213/1‑149 (MQ=255)
                              tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGatat                                                                                                                        <  2:2458027/149‑1 (MQ=255)
                                  ggCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGttt                                                                                                                    <  2:611180/149‑1 (MQ=255)
                                     ggTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgt                                                                                                                 <  2:3482897/149‑1 (MQ=255)
                                      gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtt                                                                                                                >  2:3517706/1‑149 (MQ=255)
                                       tAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtta                                                                                                               <  1:4355526/149‑1 (MQ=255)
                                        aaaCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttag                                                                                                              >  2:2819860/1‑149 (MQ=255)
                                         aaCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                             <  1:1072290/149‑1 (MQ=255)
                                           cGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagtta                                                                                                           <  2:1943930/149‑1 (MQ=255)
                                            gTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat                                                                                                          >  2:1585004/1‑149 (MQ=255)
                                            gTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGATGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat                                                                                                          >  2:1383604/1‑149 (MQ=255)
                                            gTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGAAGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat                                                                                                          >  1:1625332/1‑149 (MQ=255)
                                             tAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTTCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                         <  2:2609814/149‑1 (MQ=255)
                                             tAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                         <  1:4137342/149‑1 (MQ=255)
                                               ggTGGCTTTGGGTTATTACCCGGATCGCTGGAAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATAACGATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACt                                                                                                       >  1:154441/1‑149 (MQ=255)
                                                 tGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTcc                                                                                                     <  2:3736018/149‑1 (MQ=255)
                                                  ggCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTccc                                                                                                    <  2:1291640/149‑1 (MQ=255)
                                                      ttGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGcc                                                                                                <  1:2180893/149‑1 (MQ=255)
                                                         ggTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAAc                                                                                             <  1:4159589/149‑1 (MQ=255)
                                                           ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTa                                                                                           <  1:2908030/149‑1 (MQ=255)
                                                           ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTa                                                                                           >  2:843557/1‑149 (MQ=255)
                                                               taCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGt                                                                                       <  1:131197/149‑1 (MQ=255)
                                                                aCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAATCCCGGCCAACTAggggc                                                                                      >  1:4455974/1‑147 (MQ=255)
                                                                    ggATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGc                                                                                  >  1:3640539/1‑149 (MQ=255)
                                                                        cGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATAGGTTTTGTTAGTTATACTCCCGGCCAACGAGGGTCTGGCAAAt                                                                              >  1:981519/1‑149 (MQ=255)
                                                                         gCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATa                                                                             <  1:4167458/149‑1 (MQ=255)
                                                                         gCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATa                                                                             <  1:4526488/149‑1 (MQ=255)
                                                                           tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaa                                                                           <  1:1927977/149‑1 (MQ=255)
                                                                           tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACCCCCGGCCAACTAGGGTCTGGCAAATaaa                                                                           <  2:3358892/149‑1 (MQ=255)
                                                                              cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                        <  2:3888541/149‑1 (MQ=255)
                                                                              cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                        <  1:4043427/149‑1 (MQ=255)
                                                                              cAAATGTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                        <  2:2900625/149‑1 (MQ=255)
                                                                                aaTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                      >  1:325776/1‑149 (MQ=255)
                                                                                aaTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                      <  2:3428844/149‑1 (MQ=255)
                                                                                 aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGc                                                                     >  2:1844476/1‑149 (MQ=255)
                                                                                    tGATTGCGCTACTTATGGCGATCGATCTCCGCATCTGGTGTCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGTGCGGCGTTGGTGGGTTATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGa                                                                  <  2:4633436/149‑1 (MQ=255)
                                                                                       ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                               >  2:4496771/1‑149 (MQ=255)
                                                                                       ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                               >  2:177965/1‑149 (MQ=255)
                                                                                       ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                               >  2:2061229/1‑149 (MQ=255)
                                                                                            cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                          >  2:1130484/1‑149 (MQ=255)
                                                                                            cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                          >  2:3024619/1‑149 (MQ=255)
                                                                                            cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTAATAAATACCTATGCTGTTTTGGGCGGCGTTGGTGTGATATGTTTTGTTAGTTATACTACCGGCCAAATACTGTATGGCAAAAAAAAAAGGCGTATTATGAGt                                                          >  1:1075495/1‑149 (MQ=255)
                                                                                             tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCGGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTc                                                         >  1:1350107/1‑149 (MQ=255)
                                                                                             caCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTc                                                         >  2:2014043/2‑149 (MQ=255)
                                                                                              aCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTcc                                                        <  2:2994726/149‑1 (MQ=255)
                                                                                                gTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGGTGGTGGGCTATGTTTTGTTAGTTTTACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                      >  1:4594377/1‑149 (MQ=255)
                                                                                                 tATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                     <  1:1454979/149‑1 (MQ=255)
                                                                                                 tATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                     <  1:1022269/149‑1 (MQ=255)
                                                                                                 tATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCGGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTATTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                     >  1:1367687/1‑149 (MQ=255)
                                                                                                  aTGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCt                                                    <  2:650113/149‑1 (MQ=255)
                                                                                                   tGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                   >  2:3426452/1‑149 (MQ=255)
                                                                                                     gCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtttt                                                 <  1:550529/149‑1 (MQ=255)
                                                                                                       gAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                               <  1:1478783/149‑1 (MQ=255)
                                                                                                        aaCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                              <  1:4331851/149‑1 (MQ=255)
                                                                                                        aaCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTTTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                              >  1:616319/1‑149 (MQ=255)
                                                                                                            aTCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGTTATTTTTTGTTAGTTATAATCCCGGCCAACTAGGGTCTGGCAAAAAAAAAAGGCGGATTATGATTCCGCCTTTTGCTTTaa                                          >  1:845054/1‑149 (MQ=255)
                                                                                                            aTCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTaa                                          <  1:3391284/149‑1 (MQ=255)
                                                                                                            aTCGCCGCATCTGGTGGCGTGTCCAGCTCTATTAAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGGCTGGCAAATAAAAAAGGCGGATTAGGAGGCCGCCGTTTGCTTTaa                                          >  1:1791082/1‑149 (MQ=255)
                                                                                                             tCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAg                                         <  2:868562/149‑1 (MQ=255)
                                                                                                                    aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                                  <  2:3825175/149‑1 (MQ=255)
                                                                                                                              gTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                        <  1:2755813/149‑1 (MQ=255)
                                                                                                                               tttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                       >  2:2686649/1‑149 (MQ=255)
                                                                                                                                 tCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                     <  2:1364910/149‑1 (MQ=255)
                                                                                                                                 tCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                     <  2:3290905/149‑1 (MQ=255)
                                                                                                                                     tctcTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc                 >  1:3759072/1‑149 (MQ=255)
                                                                                                                                     tctcTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc                 <  2:3172787/149‑1 (MQ=255)
                                                                                                                                      ctctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                <  2:4440872/149‑1 (MQ=255)
                                                                                                                                          aTTCAAGACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa            <  2:3223139/149‑1 (MQ=255)
                                                                                                                                             cAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc         <  1:2032049/149‑1 (MQ=255)
                                                                                                                                             cAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc         <  1:3444129/149‑1 (MQ=255)
                                                                                                                                              aagaCCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc        <  2:4530454/146‑1 (MQ=255)
                                                                                                                                              aaTACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc        <  1:1599956/149‑1 (MQ=255)
                                                                                                                                              aaTACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc        >  1:2973104/1‑149 (MQ=255)
                                                                                                                                              aaTACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc        <  1:2532370/149‑1 (MQ=255)
                                                                                                                                                 aCCGTTGCTGTTGTGGGCGGCGTTGGTGGGATTTGTTTTGTTAGTTATACTCCCGGCCATCTAGGGTCTGGCAAATAAAATAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGa     <  2:122557/149‑1 (MQ=255)
                                                                                                                                                    gATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg  >  2:2303365/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCG  >  CP009273/850013‑850309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑