BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	964,594	G→T	7.9%	C258F (TGT→TTT)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	964,594	0	G	T	7.9%	886.4 / 33.7	240	C258F (TGT→TTT)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand): ref base G (86/135); new base T (11/8); total (97/143)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 262 total aligned reads displayed.

CATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCA  >  CP009273/964446‑964742

cATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtg                                                                                                                                                      >  2:1147500/1‑149 (MQ=255)

 aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCATCTTGAACAAGTAGTGGCGATGGCGTGGATTGGGCATCCGCCGAGCTTTTTTGCCACGCAGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                                                                                     >  1:17333/1‑149 (MQ=255)

 aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                                                                                     >  2:1571103/1‑149 (MQ=255)

 aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                                                                                     <  2:484727/149‑1 (MQ=255)

 aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                                                                                     >  2:2873505/1‑149 (MQ=255)

 aTCTGATGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                                                                                     >  2:4684515/1‑149 (MQ=255)

   ctgctgCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGAGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGAGGCGTACAACCGGAAAAATatgt                                                                                                                                                   >  2:3261247/1‑149 (MQ=255)

    tgctgcCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTa                                                                                                                                                  <  2:223819/149‑1 (MQ=255)

    tgctgcCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTa                                                                                                                                                  >  1:3198728/1‑149 (MQ=255)

    tgctgcAGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTa                                                                                                                                                  <  2:3804011/149‑1 (MQ=255)

     gctgcCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTAc                                                                                                                                                 <  2:388180/149‑1 (MQ=255)

           gggTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTgg                                                                                                                                           >  2:3257810/1‑149 (MQ=255)

                   cGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATTTGTACCGCTGGCCGatcat                                                                                                                                   >  2:158929/1‑149 (MQ=255)

                    ggTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATTTGTACCGCTGGCCGatcatc                                                                                                                                  >  2:2543153/1‑149 (MQ=255)

                      tGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAg                                                                                                                                >  1:1185232/1‑149 (MQ=255)

                       gAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGt                                                                                                                               <  1:2032053/149‑1 (MQ=255)

                        aaTTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTc                                                                                                                              <  2:941628/149‑1 (MQ=255)

                               gTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAAc                                                                                                                       <  2:2657035/149‑1 (MQ=255)

                                 aCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCa                                                                                                                     >  1:381440/1‑149 (MQ=255)

                                    ggTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGc                                                                                                                  >  2:268710/1‑149 (MQ=255)

                                       aCGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGa                                                                                                               <  2:722145/149‑1 (MQ=255)

                                         gcgTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATg                                                                                                             <  2:4535539/149‑1 (MQ=255)

                                           gTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTc                                                                                                           <  1:4305657/149‑1 (MQ=255)

                                            ttGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCa                                                                                                          >  2:3007363/1‑149 (MQ=255)

                                              gtgACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGt                                                                                                        >  2:2203829/1‑149 (MQ=255)

                                              gtgACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGt                                                                                                        <  2:4668887/149‑1 (MQ=255)

                                               tgACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTg                                                                                                       <  2:2059710/149‑1 (MQ=255)

                                                gACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGc                                                                                                      <  1:471999/149‑1 (MQ=255)

                                                   gTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGtt                                                                                                   >  2:1808806/1‑149 (MQ=255)

                                                   gTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGtt                                                                                                   <  1:4589129/149‑1 (MQ=255)

                                                   gTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGtt                                                                                                   <  1:600535/149‑1 (MQ=255)

                                                    ttGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTg                                                                                                  >  2:3092322/1‑149 (MQ=255)

                                                    ttGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTg                                                                                                  <  1:4059065/149‑1 (MQ=255)

                                                     tGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTgg                                                                                                 >  1:280920/1‑149 (MQ=255)

                                                        tCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTaa                                                                                              <  1:3092937/149‑1 (MQ=255)

                                                        tCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTaa                                                                                              >  1:4250762/1‑149 (MQ=255)

                                                        tCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTaa                                                                                              <  1:346907/149‑1 (MQ=255)

                                                         cAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAg                                                                                             <  1:721254/149‑1 (MQ=255)

                                                           gCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAgcg                                                                                           >  1:139723/1‑149 (MQ=255)

                                                           gCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAgcg                                                                                           >  1:2532846/1‑149 (MQ=255)

                                                                aaCATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCggg                                                                                      <  2:4304587/149‑1 (MQ=255)

                                                                  cATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCa                                                                                    >  2:3529963/1‑149 (MQ=255)

                                                                     gTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTTGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAAc                                                                                 <  2:617259/149‑1 (MQ=255)

                                                                      tAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACg                                                                                >  2:1205622/1‑149 (MQ=255)

                                                                      tAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACg                                                                                >  2:1205508/1‑149 (MQ=255)

                                                                          ggCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg                                                                            <  1:3451887/149‑1 (MQ=255)

                                                                           gCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGt                                                                           >  1:3913346/1‑149 (MQ=255)

                                                                              aTGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAAt                                                                        <  2:3132102/149‑1 (MQ=255)

                                                                                  cGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACt                                                                    <  2:1073667/149‑1 (MQ=255)

                                                                                   ggggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTg                                                                   >  1:3760684/1‑149 (MQ=255)

                                                                                   ggggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTg                                                                   <  2:2738187/149‑1 (MQ=255)

                                                                                    gggATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGa                                                                  >  1:1708727/1‑149 (MQ=255)

                                                                                       aTTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGaaaa                                                               >  2:2865752/1‑149 (MQ=255)

                                                                                          gggCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGa                                                            <  2:1852835/149‑1 (MQ=255)

                                                                                           ggCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAt                                                           <  1:3934124/149‑1 (MQ=255)

                                                                                           ggCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAt                                                           >  2:2028001/1‑149 (MQ=255)

                                                                                           ggCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAt                                                           >  2:1303391/1‑149 (MQ=255)

                                                                                            gCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                                                          <  1:377484/149‑1 (MQ=255)

                                                                                            gCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATg                                                          <  1:3787366/149‑1 (MQ=255)

                                                                                              aTCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCg                                                        <  2:1066009/149‑1 (MQ=255)

                                                                                               tCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCgg                                                       <  1:1173673/149‑1 (MQ=255)

                                                                                                  gccgcGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGa                                                    <  2:2340611/149‑1 (MQ=255)

                                                                                                    cgcgCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaaa                                                  <  1:1095040/149‑1 (MQ=255)

                                                                                                    cgcgCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGaaa                                                  <  1:930222/149‑1 (MQ=255)

                                                                                                     gcgcTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATTTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAAt                                                 <  2:3443975/149‑1 (MQ=255)

                                                                                                     gcgcTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGGTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAGGCGGTGaact                                                 >  2:1418000/1‑147 (MQ=255)

                                                                                                      cgcTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATg                                                <  2:2662807/149‑1 (MQ=255)

                                                                                                         ttttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCg                                             >  1:4132149/1‑149 (MQ=255)

                                                                                                         ttttttGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCg                                             <  2:3488181/149‑1 (MQ=255)

                                                                                                               gCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCtt                                       <  1:2031792/149‑1 (MQ=255)

                                                                                                               gCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCtt                                       <  1:23560/149‑1 (MQ=255)

                                                                                                                 cACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTg                                     <  2:3947344/149‑1 (MQ=255)

                                                                                                                       gAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGAGGCGGTGAAATGCCGGGCCTTTGCagaag                               >  2:1533265/1‑149 (MQ=255)

                                                                                                                           aTGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGaaaa                           <  1:1068213/149‑1 (MQ=255)

                                                                                                                              tgtgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATtg                        <  1:4456451/149‑1 (MQ=255)

                                                                                                                              tgtgGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATtg                        <  1:3605621/149‑1 (MQ=255)

                                                                                                                                 ggCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATtggtg                     <  2:2165820/149‑1 (MQ=255)

                                                                                                                                  gCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATtggtgg                    <  2:1338852/149‑1 (MQ=255)

                                                                                                                                     tACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAt                 >  1:48192/1‑149 (MQ=255)

                                                                                                                                      aCAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAtt                >  2:1971381/1‑149 (MQ=255)

                                                                                                                                      aCAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAtt                <  1:4188625/149‑1 (MQ=255)

                                                                                                                                       cAACCGGAAAAATTTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAttt               <  2:3824093/149‑1 (MQ=255)

                                                                                                                                       cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAttt               >  1:695442/1‑149 (MQ=255)

                                                                                                                                       cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAttt               <  2:4167388/149‑1 (MQ=255)

                                                                                                                                       cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAttt               <  1:1837694/149‑1 (MQ=255)

                                                                                                                                       cAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTAttt               <  1:4228434/149‑1 (MQ=255)

                                                                                                                                        aaCCGGAAAAATTTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTg              <  1:3870552/149‑1 (MQ=255)

                                                                                                                                        aaCCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTg              >  1:2783240/1‑149 (MQ=255)

                                                                                                                                         aCCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGc             <  1:268710/149‑1 (MQ=255)

                                                                                                                                          ccGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGcc            >  2:3771540/1‑149 (MQ=255)

                                                                                                                                           cGGAAAAATTTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCt           >  1:1523334/1‑149 (MQ=255)

                                                                                                                                             gAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGt         >  1:3812223/1‑149 (MQ=255)

                                                                                                                                              aaaaaTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTa        <  1:2482835/149‑1 (MQ=255)

                                                                                                                                               aaaaTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAg       >  2:4431672/1‑149 (MQ=255)

                                                                                                                                                 aaTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGAc     >  2:2889118/1‑149 (MQ=255)

                                                                                                                                                 aaTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGAc     <  1:766252/149‑1 (MQ=255)

                                                                                                                                                  aTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGACAATTGGTGGTATTTGCCTGTAGACg    >  2:4214798/1‑149 (MQ=255)

                                                                                                                                                    gtgtACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGca  <  1:1571103/149‑1 (MQ=255)

                                                                                                                                                    gtgtACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGca  >  1:528740/1‑149 (MQ=255)

                                                                                                                                                    gtgtACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGca  <  2:856161/149‑1 (MQ=255)

CATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCA  >  CP009273/964446‑964742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`