Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 968,415 G→T 100% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273968,4150GT99.3% 958.1 / ‑1.9 283S148* (TCG→TAG) ycbCDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (133/148);  total (134/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 283 total aligned reads displayed.

AAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAAT  >  CP009273/968269‑968559
                                                                                                                                                  |                                                                                                                                                
aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAt                                                                                                                                                <  1:3361935/149‑1 (MQ=255)
aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAt                                                                                                                                                >  1:2606261/1‑149 (MQ=255)
   aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgc                                                                                                                                             <  1:2062987/149‑1 (MQ=255)
        atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAc                                                                                                                                        <  1:2104552/149‑1 (MQ=255)
             ccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTgg                                                                                                                                   >  1:434714/1‑149 (MQ=255)
             ccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCCGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTCTTGCGCAACTCTgg                                                                                                                                   >  1:2827934/1‑149 (MQ=255)
               gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCa                                                                                                                                 >  2:42694/1‑149 (MQ=255)
                  gCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCt                                                                                                                              >  1:1699825/1‑149 (MQ=255)
                    aGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTAc                                                                                                                            <  2:1585377/149‑1 (MQ=255)
                     gATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACt                                                                                                                           >  2:2247189/1‑149 (MQ=255)
                      aTGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACtt                                                                                                                          >  1:1966609/1‑149 (MQ=255)
                       tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTc                                                                                                                         >  1:765906/1‑149 (MQ=255)
                       tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTc                                                                                                                         >  1:3349606/1‑149 (MQ=255)
                       tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTc                                                                                                                         >  1:356771/1‑149 (MQ=255)
                       tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTc                                                                                                                         <  2:175424/149‑1 (MQ=255)
                        gtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTcc                                                                                                                        <  1:4211507/149‑1 (MQ=255)
                        gtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTcc                                                                                                                        <  1:1605488/149‑1 (MQ=255)
                         tgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCg                                                                                                                       >  1:3082885/1‑149 (MQ=255)
                           aTGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCt                                                                                                                     <  1:2688199/149‑1 (MQ=255)
                               ggAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTAc                                                                                                                 <  1:3326331/149‑1 (MQ=255)
                                gAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACt                                                                                                                >  2:473236/1‑149 (MQ=255)
                                     cACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCg                                                                                                           >  1:998273/1‑149 (MQ=255)
                                      aCCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGt                                                                                                          >  1:497367/1‑149 (MQ=255)
                                           cagGAAAGGGGCGTCACCACTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTgg                                                                                                     <  2:3065153/149‑1 (MQ=255)
                                            agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTCTTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGt                                                                                                    >  2:1669887/1‑149 (MQ=255)
                                              gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttt                                                                                                  >  1:3638604/1‑149 (MQ=255)
                                              gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttt                                                                                                  >  1:3017565/1‑149 (MQ=255)
                                               aaaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                 <  1:4229473/149‑1 (MQ=255)
                                               aaaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                 >  1:2749942/1‑149 (MQ=255)
                                               aaaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                 <  1:3683311/149‑1 (MQ=255)
                                                 aGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTg                                                                                               >  2:4465731/1‑149 (MQ=255)
                                                    ggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTa                                                                                            <  2:344501/149‑1 (MQ=255)
                                                       gTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTCTTGGTTTTTGCTACGc                                                                                         >  1:70557/1‑149 (MQ=255)
                                                         cACCAATCGCCTGTGTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCt                                                                                       <  2:4021167/149‑1 (MQ=255)
                                                                   cTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGa                                                                             >  1:3950181/1‑149 (MQ=255)
                                                                    tGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGAt                                                                            >  1:1254139/1‑149 (MQ=255)
                                                                     gtgtCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTCCGTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATc                                                                           <  2:4591200/146‑1 (MQ=255)
                                                                         cACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtt                                                                       <  2:4428948/149‑1 (MQ=255)
                                                                         cACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtt                                                                       <  1:974956/149‑1 (MQ=255)
                                                                          aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGttt                                                                      <  2:2129536/149‑1 (MQ=255)
                                                                          aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGttt                                                                      <  2:4555180/149‑1 (MQ=255)
                                                                          aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGttt                                                                      <  2:3525195/149‑1 (MQ=255)
                                                                           ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                     >  1:3081266/1‑149 (MQ=255)
                                                                           ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                     >  1:4535472/1‑149 (MQ=255)
                                                                           ctgctgCAGCTTATTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                     <  2:3236011/149‑1 (MQ=255)
                                                                            tgctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTACTACGCCTCCCGTGAAGATCAGTTTTg                                                                    >  1:2545242/1‑149 (MQ=255)
                                                                                gcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATcc                                                                >  2:3084499/1‑149 (MQ=255)
                                                                                  aGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCg                                                              >  1:2406296/1‑149 (MQ=255)
                                                                                   gCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                             >  2:1934310/1‑149 (MQ=255)
                                                                                   gCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                             >  2:651839/1‑149 (MQ=255)
                                                                                   gCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                             >  2:1637338/1‑149 (MQ=255)
                                                                                    cttcttcttCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGa                                                            >  2:3299334/1‑149 (MQ=255)
                                                                                    cttcttcttCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGa                                                            <  1:4045573/149‑1 (MQ=255)
                                                                                      tcttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtt                                                          <  2:380282/149‑1 (MQ=255)
                                                                                      tcttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtt                                                          >  1:630927/1‑149 (MQ=255)
                                                                                       cttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAttt                                                         <  2:68871/149‑1 (MQ=255)
                                                                                       cttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAttt                                                         >  1:3509630/1‑149 (MQ=255)
                                                                                       cttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAttt                                                         <  2:2969368/149‑1 (MQ=255)
                                                                                        ttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtttt                                                        >  2:2017351/1‑149 (MQ=255)
                                                                                        ttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATAGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtttt                                                        <  2:2328878/149‑1 (MQ=255)
                                                                                         tcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTc                                                       <  2:2749942/149‑1 (MQ=255)
                                                                                         tcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTc                                                       <  1:933673/149‑1 (MQ=255)
                                                                                           ttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgc                                                     <  2:1127067/149‑1 (MQ=255)
                                                                                           ttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgc                                                     <  1:2274086/149‑1 (MQ=255)
                                                                                             cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCgcgc                                                   <  2:3446523/149‑1 (MQ=255)
                                                                                             cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCgcgc                                                   <  2:847910/149‑1 (MQ=255)
                                                                                             cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCgcgc                                                   <  2:3322253/149‑1 (MQ=255)
                                                                                              ggTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGcc                                                  <  1:4064882/149‑1 (MQ=255)
                                                                                               gTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCa                                                 >  2:1372952/1‑149 (MQ=255)
                                                                                               gTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCa                                                 >  1:659729/1‑149 (MQ=255)
                                                                                                tATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCAt                                                >  1:1196632/1‑149 (MQ=255)
                                                                                                tATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTGTTGATCCCGGATTTTCGCGCCAt                                                >  1:1647207/1‑149 (MQ=255)
                                                                                                 aTCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATa                                               >  2:4049999/1‑149 (MQ=255)
                                                                                                  tCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATaa                                              <  2:4038882/149‑1 (MQ=255)
                                                                                                    ttttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGc                                            <  1:3488911/149‑1 (MQ=255)
                                                                                                        ggCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                        >  2:3195350/1‑149 (MQ=255)
                                                                                                        ggCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                        <  1:3777410/149‑1 (MQ=255)
                                                                                                            aaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                    >  2:4072883/1‑149 (MQ=255)
                                                                                                             aTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtt                                   >  1:2851301/1‑149 (MQ=255)
                                                                                                                 aGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcatt                               <  1:1247438/149‑1 (MQ=255)
                                                                                                                 aGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcatt                               <  1:4008329/149‑1 (MQ=255)
                                                                                                                  gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattc                              <  1:4465731/149‑1 (MQ=255)
                                                                                                                  gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattc                              >  1:4770651/1‑149 (MQ=255)
                                                                                                                  gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattc                              >  1:3205979/1‑149 (MQ=255)
                                                                                                                   ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                             >  1:180530/1‑149 (MQ=255)
                                                                                                                   ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                             <  1:1460238/149‑1 (MQ=255)
                                                                                                                   ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                             <  2:3158833/149‑1 (MQ=255)
                                                                                                                     tGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAgg                           <  2:1477937/149‑1 (MQ=255)
                                                                                                                       aTAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCg                         <  1:3154085/149‑1 (MQ=255)
                                                                                                                         aaTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTg                       >  2:3589841/1‑149 (MQ=255)
                                                                                                                             tGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                   >  1:243155/1‑149 (MQ=255)
                                                                                                                             tGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                   <  2:2901103/149‑1 (MQ=255)
                                                                                                                               cTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGAc                 <  1:1077717/149‑1 (MQ=255)
                                                                                                                                  gcgcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGt              <  2:1318063/149‑1 (MQ=255)
                                                                                                                                    gcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGATa            >  2:3963567/1‑149 (MQ=255)
                                                                                                                                     cgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTAt           <  2:2402207/149‑1 (MQ=255)
                                                                                                                                            cccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaa    >  1:1550800/1‑149 (MQ=255)
                                                                                                                                            cccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaa    >  2:3666076/1‑149 (MQ=255)
                                                                                                                                             ccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaaa   <  2:603424/149‑1 (MQ=255)
                                                                                                                                             ccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaaa   >  2:1070852/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAAT  >  CP009273/968269‑968559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑