BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	987,103	C→T	10.2%	I342I (ATC→ATT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	987,103	0	C	T	10.2%	969.4 / 69.9	294	I342I (ATC→ATT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base C (144/120); new base T (18/12); total (162/132)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 299 total aligned reads displayed.

GCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCC  >  CP009273/986955‑987251

gcgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATc                                                                                                                                                      >  1:3478148/1‑149 (MQ=255)

gcgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATc                                                                                                                                                      <  2:1413078/149‑1 (MQ=255)

gcgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATc                                                                                                                                                      >  1:3079199/1‑149 (MQ=255)

 cgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTACAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCa                                                                                                                                                     >  1:1575585/1‑149 (MQ=255)

  gTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaa                                                                                                                                                    <  2:1788948/149‑1 (MQ=255)

  gTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaa                                                                                                                                                    >  1:38606/1‑149 (MQ=255)

  gTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaa                                                                                                                                                    <  2:4008559/149‑1 (MQ=255)

      tCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaataat                                                                                                                                                <  2:2558971/149‑1 (MQ=255)

          ccATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTAc                                                                                                                                            >  1:669557/1‑149 (MQ=255)

           cATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCCATAATGTACg                                                                                                                                           >  1:2934054/1‑149 (MQ=255)

            aTGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGc                                                                                                                                          >  2:2886614/1‑149 (MQ=255)

             tGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCa                                                                                                                                         >  1:1537478/1‑149 (MQ=255)

              gAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCAc                                                                                                                                        >  1:3394213/1‑149 (MQ=255)

                atatTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCa                                                                                                                                      <  2:1875653/149‑1 (MQ=255)

                 tatTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCAt                                                                                                                                     >  1:528076/1‑149 (MQ=255)

                 tatTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCAt                                                                                                                                     >  2:2192315/1‑149 (MQ=255)

                  atTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATg                                                                                                                                    >  1:1849304/1‑149 (MQ=255)

                   tttCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgc                                                                                                                                   >  1:3936513/1‑149 (MQ=255)

                   tttCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgc                                                                                                                                   >  1:2338097/1‑149 (MQ=255)

                   tttCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgc                                                                                                                                   >  1:514554/1‑149 (MQ=255)

                         tAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGAt                                                                                                                             <  1:2008158/149‑1 (MQ=255)

                         tAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGAt                                                                                                                             <  2:3984163/149‑1 (MQ=255)

                         tAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTGGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGAt                                                                                                                             >  2:3410475/1‑149 (MQ=255)

                              ggACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAg                                                                                                                        <  2:205530/149‑1 (MQ=255)

                                aCCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGtt                                                                                                                      <  2:4601232/149‑1 (MQ=255)

                                aCCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGtt                                                                                                                      <  2:685671/149‑1 (MQ=255)

                                 ccGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGttt                                                                                                                     <  1:2378871/149‑1 (MQ=255)

                                 ccGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGttt                                                                                                                     <  2:4285224/149‑1 (MQ=255)

                                  cGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTg                                                                                                                    >  1:2692252/1‑149 (MQ=255)

                                   ggTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGc                                                                                                                   <  2:3367456/149‑1 (MQ=255)

                                    gTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCa                                                                                                                  >  1:466058/1‑149 (MQ=255)

                                         cGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCagaaga                                                                                                             >  1:2320777/1‑149 (MQ=255)

                                          gAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGAc                                                                                                            <  2:3326313/149‑1 (MQ=255)

                                           aGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGCTTGCAGTTTGCAGAAGACg                                                                                                           >  2:3354720/1‑149 (MQ=255)

                                            gTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGc                                                                                                          >  2:3023402/1‑149 (MQ=255)

                                              gACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCa                                                                                                        >  2:730401/1‑149 (MQ=255)

                                              gACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCa                                                                                                        <  1:4446135/149‑1 (MQ=255)

                                               aCCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAg                                                                                                       >  2:1340751/1‑149 (MQ=255)

                                                ccTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGc                                                                                                      >  2:1980049/1‑149 (MQ=255)

                                                 cTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGcc                                                                                                     >  2:3283302/1‑149 (MQ=255)

                                                   gTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCg                                                                                                   >  2:1562471/1‑149 (MQ=255)

                                                      gcgACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATg                                                                                                >  1:1077683/1‑149 (MQ=255)

                                                         aCTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGgcg                                                                                             >  2:2325113/1‑149 (MQ=255)

                                                            ggTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCAc                                                                                          >  1:1400192/1‑149 (MQ=255)

                                                               tCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCg                                                                                       <  2:3040444/149‑1 (MQ=255)

                                                                  aGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATc                                                                                    >  1:2482483/1‑149 (MQ=255)

                                                                  aGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATc                                                                                    <  2:1814503/149‑1 (MQ=255)

                                                                   gCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATcc                                                                                   >  2:4786744/1‑149 (MQ=255)

                                                                     tCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGc                                                                                 >  1:3985174/1‑149 (MQ=255)

                                                                            gAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGata                                                                          >  1:797508/1‑149 (MQ=255)

                                                                               agaagGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATgg                                                                       <  2:2692252/149‑1 (MQ=255)

                                                                               agaagGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATgg                                                                       <  2:514554/149‑1 (MQ=255)

                                                                                  agGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCa                                                                    >  1:4233569/1‑149 (MQ=255)

                                                                                   ggTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCAt                                                                   <  2:4443722/149‑1 (MQ=255)

                                                                                    gTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCAtt                                                                  >  1:1217746/1‑149 (MQ=255)

                                                                                    gTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCAtt                                                                  >  1:3606460/1‑149 (MQ=255)

                                                                                        aaCCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTgaga                                                              <  1:3238737/149‑1 (MQ=255)

                                                                                         aCCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGAt                                                             >  2:4373122/1‑149 (MQ=255)

                                                                                         aCCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGAt                                                             >  1:4520805/1‑149 (MQ=255)

                                                                                          ccGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATg                                                            <  1:2134748/149‑1 (MQ=255)

                                                                                            gTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGaa                                                          >  1:3261543/1‑149 (MQ=255)

                                                                                            gTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGaa                                                          >  1:3433988/1‑149 (MQ=255)

                                                                                              cTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGaaca                                                        <  2:643794/149‑1 (MQ=255)

                                                                                                tCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGaacaac                                                      >  1:1316959/1‑149 (MQ=255)

                                                                                                  cgcgATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACtt                                                    <  1:3347664/149‑1 (MQ=255)

                                                                                                   gcgATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTc                                                   >  1:2202819/1‑149 (MQ=255)

                                                                                                     gATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTa                                                 >  2:1225809/1‑149 (MQ=255)

                                                                                                     gATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTa                                                 >  2:1357675/1‑149 (MQ=255)

                                                                                                       tCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTaca                                               <  2:1008220/149‑1 (MQ=255)

                                                                                                        cAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTacac                                              <  1:2108437/149‑1 (MQ=255)

                                                                                                         aGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACAcc                                             >  1:1690846/1‑149 (MQ=255)

                                                                                                         aGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCACACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACAcc                                             <  2:2842003/149‑1 (MQ=255)

                                                                                                          ggAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACAccc                                            >  1:4752013/1‑149 (MQ=255)

                                                                                                           gAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCt                                           >  2:2176902/1‑149 (MQ=255)

                                                                                                           gAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCt                                           >  2:3493040/1‑149 (MQ=255)

                                                                                                           gAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCt                                           <  1:749394/149‑1 (MQ=255)

                                                                                                             gTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGa                                         <  2:3106492/149‑1 (MQ=255)

                                                                                                              ttCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGAc                                        >  1:1001867/1‑149 (MQ=255)

                                                                                                              ttCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGAc                                        >  2:1614588/1‑149 (MQ=255)

                                                                                                                       gACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACga                               <  1:494523/149‑1 (MQ=255)

                                                                                                                           ttGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAg                           <  1:4362014/149‑1 (MQ=255)

                                                                                                                           ttGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAg                           <  1:4107835/149‑1 (MQ=255)

                                                                                                                             ggTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAggg                         >  1:4590009/1‑149 (MQ=255)

                                                                                                                               ttCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTACAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGgcg                       >  2:1714749/1‑149 (MQ=255)

                                                                                                                               ttCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTACAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGgcg                       >  2:4104389/1‑149 (MQ=255)

                                                                                                                                      gcAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGt                <  2:1186352/149‑1 (MQ=255)

                                                                                                                                         gTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGAt             >  2:197055/1‑149 (MQ=255)

                                                                                                                                         gTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGAt             <  1:1933856/149‑1 (MQ=255)

                                                                                                                                         gTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGAt             <  1:4323703/149‑1 (MQ=255)

                                                                                                                                           tAACCGCATTAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTc           >  1:2590571/1‑149 (MQ=255)

                                                                                                                                           tAACCGCATCACTAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTTCGAGAAGGGCGCGGAAGTGATTc           <  1:857826/149‑1 (MQ=255)

                                                                                                                                            aaCCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCg          >  2:3944498/1‑149 (MQ=255)

                                                                                                                                             aCCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGc         >  1:2823455/1‑149 (MQ=255)

                                                                                                                                             aCCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGc         >  1:831918/1‑149 (MQ=255)

                                                                                                                                               cGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCAt       <  2:41356/149‑1 (MQ=255)

                                                                                                                                               cGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCAt       <  1:2972407/149‑1 (MQ=255)

                                                                                                                                                 cATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGa     <  1:581474/149‑1 (MQ=255)

                                                                                                                                                  aTCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGAt    >  2:4554702/1‑149 (MQ=255)

                                                                                                                                                   tCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATc   <  2:3924796/149‑1 (MQ=255)

                                                                                                                                                    cAATAATGGACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATcc  <  2:3734805/149‑1 (MQ=255)

GCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCC  >  CP009273/986955‑987251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`