| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 97,521 | C→A | 100% | A90A (GCC→GCA) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 97,521 | 0 | C | A | 99.3% | 911.3 / ‑2.0 | 269 | A90A (GCC→GCA) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (124/143); total (126/143) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 269 total aligned reads displayed.
AAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCC > CP009273/97376‑97665 | aaGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGAt < 1:305494/149‑1 (MQ=255) aGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATa < 1:2834614/149‑1 (MQ=255) ggTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATaa < 2:3788572/149‑1 (MQ=255) ggTTATCAGATCAGTGGTTCCGATTGAGCGCCAAATCCGGTCACGCAGCAGTTACTGACTCTGGGTGCGACGATTTATTTCCACCATCGCCCGGAAACCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATaa < 2:1969202/149‑1 (MQ=255) gTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTACAACGCCATTTCTGCAGATAAc > 1:1631228/1‑149 (MQ=255) agatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaa > 1:2469235/1‑149 (MQ=255) agatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaa > 2:3310615/1‑149 (MQ=255) gatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAACACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaaa > 2:3329124/1‑149 (MQ=255) gatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaaa > 2:1785902/1‑149 (MQ=255) cagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTg < 1:3030780/149‑1 (MQ=255) gTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGCTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTc < 1:2750874/149‑1 (MQ=255) tGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcg > 2:2345805/1‑149 (MQ=255) ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc < 1:2860885/149‑1 (MQ=255) ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc < 2:4723/149‑1 (MQ=255) ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc < 1:3858830/149‑1 (MQ=255) ttCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccg > 2:1390607/1‑149 (MQ=255) tCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccgc < 2:3540785/149‑1 (MQ=255) gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCa > 2:4464926/1‑149 (MQ=255) aTTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGACATTGTCGCCGCTCAt > 2:1639687/1‑149 (MQ=255) aTTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt < 2:3617005/149‑1 (MQ=255) aTTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt < 2:1280344/149‑1 (MQ=255) tttAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATg > 1:1163298/1‑149 (MQ=255) tttAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATg < 2:2740368/149‑1 (MQ=255) aaaTCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCGCATGAAGcgcgga > 1:4409447/1‑147 (MQ=255) aaaTCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTa > 1:1642827/1‑149 (MQ=255) tCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTc > 2:4142410/1‑149 (MQ=255) tCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAGTGTCGCCGCTCATGAAGCGCGTATTc > 1:4586337/1‑149 (MQ=255) cGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCg < 1:491353/149‑1 (MQ=255) ggTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCgg < 1:474807/149‑1 (MQ=255) gTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGt < 1:3732922/149‑1 (MQ=255) cACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGa > 2:1840111/1‑149 (MQ=255) aCGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGAt > 2:2698685/1‑149 (MQ=255) aCGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGAt < 1:2431513/149‑1 (MQ=255) cGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATc < 1:4295531/149‑1 (MQ=255) gcagcaGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATcc > 2:1367562/1‑149 (MQ=255) agcagTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgt > 1:1918648/1‑149 (MQ=255) agTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtcgt < 2:1451858/149‑1 (MQ=255) agTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtcgt < 2:1452004/149‑1 (MQ=255) agTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtcgt > 2:3541996/1‑149 (MQ=255) gTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTg > 1:3740435/1‑149 (MQ=255) gTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCAATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTg > 2:2740159/1‑149 (MQ=255) gAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGaaa < 2:3950864/149‑1 (MQ=255) aTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATg > 1:919378/1‑149 (MQ=255) tCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGCAATgc > 2:500497/1‑149 (MQ=255) cTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgct < 2:2152902/149‑1 (MQ=255) gggTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctgg < 1:2228363/149‑1 (MQ=255) ggTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggc < 2:4766091/149‑1 (MQ=255) ggTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggc < 1:1347492/149‑1 (MQ=255) gTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGGTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggct < 2:1141177/149‑1 (MQ=255) gCGACGATTTATTTCAACCATCGCCCGGAAATCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTTATCCGTCGTGCCGAAATGCTGGCTGa > 2:1598457/1‑149 (MQ=255) gCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGa < 2:69818/149‑1 (MQ=255) cgacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAg < 2:3081930/149‑1 (MQ=255) cgacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAg < 1:784071/149‑1 (MQ=255) cgacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAg < 1:2345805/149‑1 (MQ=255) gacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGt > 2:830325/1‑149 (MQ=255) acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt > 2:1871324/1‑149 (MQ=255) acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt > 2:420314/1‑149 (MQ=255) acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt > 1:2639940/1‑149 (MQ=255) gaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTaa < 1:2650422/149‑1 (MQ=255) atttatttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAAt < 2:254322/149‑1 (MQ=255) atttatttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAAt < 1:4148480/149‑1 (MQ=255) atttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGCTTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGt > 2:2220745/1‑149 (MQ=255) ttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGttt < 1:1446385/149‑1 (MQ=255) tCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtttt > 1:4617505/1‑149 (MQ=255) cAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTc < 2:812027/149‑1 (MQ=255) cATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTATGCAGATAAACCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAAATCTGGCTGAGTTAATTCGTTTTCGTCa > 1:2015485/1‑149 (MQ=255) cATAGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCa > 2:1781040/1‑149 (MQ=255) aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAt > 2:143064/1‑149 (MQ=255) aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAt < 2:872095/149‑1 (MQ=255) cGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAACCGGAAATTGTCGCCGCGCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATTCGTTTTAGTCATgg > 1:1609497/1‑149 (MQ=255) cGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAGGAAGCGCGTATTCCCGGGATCCGGCGTGCCGCAATGCTGGCTGAGTTAATGCGTTTTCGTCAAgg > 1:3996435/1‑149 (MQ=255) cGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTATGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATc > 1:1496089/1‑149 (MQ=255) aaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCa < 1:1148777/149‑1 (MQ=255) aaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAt > 1:3586683/1‑149 (MQ=255) gtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAACTTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGc > 1:1053065/1‑149 (MQ=255) gtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTACGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATTCGTTTTCGTCATTGCATCGCCAtttc > 1:3442323/1‑147 (MQ=255) gtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGa < 1:2298276/149‑1 (MQ=255) gtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGa > 2:4169333/1‑149 (MQ=255) gATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAAc > 2:3915241/1‑149 (MQ=255) aTGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATTAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACg > 1:2136290/1‑149 (MQ=255) tGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGc < 1:32113/149‑1 (MQ=255) gTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaa > 2:299360/1‑149 (MQ=255) ggTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaaaa < 1:3397661/149‑1 (MQ=255) gTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAAc < 2:73476/149‑1 (MQ=255) gTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAAc > 2:1528748/1‑149 (MQ=255) gTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAAc > 2:1430070/1‑149 (MQ=255) gTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAAc < 1:182709/149‑1 (MQ=255) gTCGTTGTTTCCAGCGCGATTTCAGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTACGGTTATACGTCGTGCCGAAATGCTGGCTGAGTTAATTCGTTTTCGTCATGGCATCGCCATTGCCGGAAAGCACGGCAAAAc > 2:2042956/1‑149 (MQ=255) ttgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACa > 1:2566677/1‑149 (MQ=255) tgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCTAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACaa < 2:4046266/149‑1 (MQ=255) tttCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAACACGACAacc > 2:2868428/1‑149 (MQ=255) ttCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacca < 1:969948/149‑1 (MQ=255) tCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac < 2:3441289/149‑1 (MQ=255) ccAGCGCGATTTCTGCAGATAACCCGGACATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc < 2:1556421/149‑1 (MQ=255) ccAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 1:3450172/1‑149 (MQ=255) aGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 2:3369736/1‑149 (MQ=255) gcgATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGAt < 2:1694146/149‑1 (MQ=255) gATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATgg < 2:574805/149‑1 (MQ=255) ttCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGttt < 1:3307485/149‑1 (MQ=255) tCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTc < 2:3117837/149‑1 (MQ=255) | AAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCC > CP009273/97376‑97665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |