Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 102,769 G→T 85.5% V326V (GTG→GTT)  ftsZ → GTP‑binding tubulin‑like cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273102,7690GT85.5% 586.5 / 112.8 260V326V (GTG→GTT) ftsZGTP‑binding tubulin‑like cell division protein
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base T (130/92);  total (149/111)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 260 total aligned reads displayed.

GTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATCC  >  CP009273/102623‑102914
                                                                                                                                                  |                                                                                                                                                 
gtaggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                                                 >  2:652605/1‑149 (MQ=255)
gtaggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                                                 >  1:3980544/1‑149 (MQ=255)
gtaggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                                                 >  1:3289474/1‑149 (MQ=255)
  aggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCa                                                                                                                                               >  2:3237729/1‑149 (MQ=255)
   ggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCaa                                                                                                                                              <  2:1381000/149‑1 (MQ=255)
      aaCACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATaa                                                                                                                                           <  2:1964317/149‑1 (MQ=255)
         acCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCa                                                                                                                                        <  1:4226184/149‑1 (MQ=255)
            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                                                     >  2:3424740/1‑149 (MQ=255)
            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                                                     <  2:2422206/149‑1 (MQ=255)
            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                                                     <  2:2094046/149‑1 (MQ=255)
            aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                                                     >  1:4093154/1‑149 (MQ=255)
              ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTc                                                                                                                                   <  2:1398780/149‑1 (MQ=255)
                 tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTcagc                                                                                                                                >  1:1060551/1‑149 (MQ=255)
                 tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagc                                                                                                                                >  1:980798/1‑149 (MQ=255)
                 tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagc                                                                                                                                >  1:3385478/1‑149 (MQ=255)
                  gCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagca                                                                                                                               >  2:4545164/1‑149 (MQ=255)
                     tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCCATAAGCAGGTTCAGCAGcc                                                                                                                            >  2:913983/1‑149 (MQ=255)
                      ttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCa                                                                                                                           >  2:4762978/1‑149 (MQ=255)
                       tGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAg                                                                                                                          <  2:3771123/149‑1 (MQ=255)
                           tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCCTGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                                                      >  1:824628/1‑149 (MQ=255)
                           tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                                                      >  1:3712875/1‑149 (MQ=255)
                           tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAAGGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                                                      <  1:3007837/149‑1 (MQ=255)
                            ccGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     >  2:4019337/1‑149 (MQ=255)
                            ccGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     <  1:2255616/149‑1 (MQ=255)
                               aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGAt                                                                                                                  >  1:436969/1‑149 (MQ=255)
                                cAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATc                                                                                                                 >  2:2452331/1‑149 (MQ=255)
                                cAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATc                                                                                                                 <  1:3214297/149‑1 (MQ=255)
                                cAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAAGGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATc                                                                                                                 <  1:443007/149‑1 (MQ=255)
                                 aaCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCg                                                                                                                >  1:4638995/1‑149 (MQ=255)
                                    gcgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTa                                                                                                             <  1:2417267/149‑1 (MQ=255)
                                      gACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                                                           <  1:1347427/149‑1 (MQ=255)
                                       aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACca                                                                                                          >  2:4044662/1‑149 (MQ=255)
                                        cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcag                                                                                                         >  2:4237474/1‑149 (MQ=255)
                                          gtgGTTATCGGTAATTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCTTTTTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagca                                                                                                       <  1:2056188/149‑1 (MQ=255)
                                              ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                                                                   >  1:1599483/1‑149 (MQ=255)
                                              ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                                                                   >  1:1199632/1‑149 (MQ=255)
                                              ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                                                                   >  2:3789170/1‑149 (MQ=255)
                                                 tCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTAGAGGCATGGACAAACGTCCTGAAATCACTCTGGATACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                                                >  1:4541484/1‑149 (MQ=255)
                                                    gTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGTTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAATCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATg                                                                                             >  1:477929/1‑149 (MQ=255)
                                                    gTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATg                                                                                             >  2:1588064/1‑149 (MQ=255)
                                                    gTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATg                                                                                             >  1:1003025/1‑149 (MQ=255)
                                                     tACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATgg                                                                                            >  2:782975/1‑149 (MQ=255)
                                                      aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGc                                                                                           <  2:204690/149‑1 (MQ=255)
                                                       cTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCt                                                                                          >  2:1154672/1‑149 (MQ=255)
                                                               gACCCGGAGATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAc                                                                                  <  1:574047/149‑1 (MQ=255)
                                                                 cccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAccc                                                                                <  2:3054874/149‑1 (MQ=255)
                                                                  ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCa                                                                               >  1:206693/1‑149 (MQ=255)
                                                                  ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCa                                                                               >  2:1049226/1‑149 (MQ=255)
                                                                      atatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                                                           >  2:4139146/1‑149 (MQ=255)
                                                                       tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGc                                                                          <  1:4181698/149‑1 (MQ=255)
                                                                            atgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAg                                                                     <  1:3933115/149‑1 (MQ=255)
                                                                            atgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAg                                                                     >  2:803491/1‑149 (MQ=255)
                                                                              gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGcc                                                                   <  1:2613152/149‑1 (MQ=255)
                                                                                    cTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATTGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGc                                                             <  2:1926717/149‑1 (MQ=255)
                                                                                      gcgcGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTa                                                           <  1:779358/149‑1 (MQ=255)
                                                                                      gcgcGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTa                                                           >  1:3452538/1‑149 (MQ=255)
                                                                                          gTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGt                                                       <  1:4551214/149‑1 (MQ=255)
                                                                                            aaCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                                                     >  2:3555256/1‑149 (MQ=255)
                                                                                            aaCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                                                     >  2:1710980/1‑149 (MQ=255)
                                                                                             aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGt                                                    >  1:662280/1‑149 (MQ=255)
                                                                                               cGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGa                                                  >  1:2710651/1‑149 (MQ=255)
                                                                                               cGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGa                                                  >  1:317737/1‑149 (MQ=255)
                                                                                                gttgttGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGaa                                                 <  2:4291669/149‑1 (MQ=255)
                                                                                                 ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAAt                                                >  1:1226176/1‑149 (MQ=255)
                                                                                                 ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAAt                                                >  1:2460188/1‑149 (MQ=255)
                                                                                                 ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAAt                                                >  1:1611289/1‑149 (MQ=255)
                                                                                                  tgttgCGACAGGTTTAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGTGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATg                                               <  2:4446893/149‑1 (MQ=255)
                                                                                                   gttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGa                                              <  1:2509126/149‑1 (MQ=255)
                                                                                                   gttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGa                                              <  1:3190059/149‑1 (MQ=255)
                                                                                                         aCAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgc                                        >  2:3254660/1‑149 (MQ=255)
                                                                                                         aCAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgc                                        <  1:1517448/149‑1 (MQ=255)
                                                                                                           aGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcgc                                      >  2:189315/1‑149 (MQ=255)
                                                                                                              tataGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGATGCTAAAGTCGTGAATGACAATGcgccgc                                   >  1:2921697/1‑149 (MQ=255)
                                                                                                                  ggCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAAc                               <  2:2591621/149‑1 (MQ=255)
                                                                                                                  ggCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAAc                               >  2:4078108/1‑149 (MQ=255)
                                                                                                                   gCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACt                              <  2:520204/149‑1 (MQ=255)
                                                                                                                       ggACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGa                          >  2:2294565/1‑149 (MQ=255)
                                                                                                                        gACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGaa                         <  2:2743017/149‑1 (MQ=255)
                                                                                                                         acacacGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGaaa                        <  2:3335045/149‑1 (MQ=255)
                                                                                                                         aCAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGaaa                        <  1:3822560/149‑1 (MQ=255)
                                                                                                                          cAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAag                       <  2:4387048/149‑1 (MQ=255)
                                                                                                                           aaaCGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAaga                      <  2:2213204/149‑1 (MQ=255)
                                                                                                                            aaCGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAagag                     >  2:2706074/1‑149 (MQ=255)
                                                                                                                            aaCGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAagag                     >  1:1798710/1‑149 (MQ=255)
                                                                                                                             aCGTCCTGAAATCACTCTGGTTACCAATAAGTAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGc                    >  1:3202883/1‑149 (MQ=255)
                                                                                                                               gTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCg                  <  2:3448387/149‑1 (MQ=255)
                                                                                                                                tCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  2:1429591/1‑149 (MQ=255)
                                                                                                                                tCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  1:615374/1‑149 (MQ=255)
                                                                                                                                tCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  2:1240340/1‑149 (MQ=255)
                                                                                                                                 ccTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGa                <  1:1379145/149‑1 (MQ=255)
                                                                                                                                  cTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGAt               <  2:1344392/149‑1 (MQ=255)
                                                                                                                                     aaaTCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTAt            <  2:3610194/149‑1 (MQ=255)
                                                                                                                                      aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           >  2:3035328/1‑149 (MQ=255)
                                                                                                                                      aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           >  2:3035688/1‑149 (MQ=255)
                                                                                                                                      aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           <  1:4019337/149‑1 (MQ=255)
                                                                                                                                      aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           >  1:4719019/1‑149 (MQ=255)
                                                                                                                                         cACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTgg        >  2:3736649/1‑149 (MQ=255)
                                                                                                                                          aCTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGa       >  1:1130975/1‑149 (MQ=255)
                                                                                                                                              tGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATc   >  1:4209434/1‑149 (MQ=255)
                                                                                                                                               ggTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATcc  >  1:1487809/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATCC  >  CP009273/102623‑102914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑