Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 104,561 G→T 100% A124S (GCG→TCG)  secM → regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273104,5610GT99.6% 894.0 / ‑4.1 259A124S (GCG→TCG) secMregulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (124/133);  total (124/135)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 264 total aligned reads displayed.

ACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAAC  >  CP009273/104415‑104705
                                                                                                                                                  |                                                                                                                                                
 cTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAgctcgc                                                                                                                                               <  1:2635977/149‑1 (MQ=255)
 cTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAgctcgc                                                                                                                                               <  1:243973/149‑1 (MQ=255)
   aTTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctg                                                                                                                                             >  1:4191053/1‑149 (MQ=255)
   aTTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctg                                                                                                                                             >  1:2015336/1‑149 (MQ=255)
     tCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctgct                                                                                                                                           <  1:283469/149‑1 (MQ=255)
       cGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                         >  2:948201/1‑149 (MQ=255)
        gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAc                                                                                                                                        >  2:1125997/1‑149 (MQ=255)
         ttGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAcc                                                                                                                                       >  1:257969/1‑149 (MQ=255)
         ttGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAcc                                                                                                                                       >  2:2217062/1‑149 (MQ=255)
            aTTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAg                                                                                                                                    <  1:2156476/149‑1 (MQ=255)
             ttACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAgg                                                                                                                                   >  1:1547002/1‑149 (MQ=255)
                 tGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAgg                                                                                                                               <  1:1005323/149‑1 (MQ=255)
                   gCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCa                                                                                                                             <  1:632663/149‑1 (MQ=255)
                   gCATCAACATGCCAGTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCa                                                                                                                             <  2:3409957/149‑1 (MQ=255)
                         aCATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGt                                                                                                                       >  1:4570758/1‑149 (MQ=255)
                          cATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAAGGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTc                                                                                                                      <  2:2824509/149‑1 (MQ=255)
                            tGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTg                                                                                                                    <  2:1375002/149‑1 (MQ=255)
                             gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGa                                                                                                                   >  1:3045791/1‑149 (MQ=255)
                             gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGa                                                                                                                   >  2:640162/1‑149 (MQ=255)
                             gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGa                                                                                                                   >  2:640701/1‑149 (MQ=255)
                               cATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                                 >  2:2990991/1‑149 (MQ=255)
                                aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaaa                                                                                                                <  2:3674632/149‑1 (MQ=255)
                                aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaaa                                                                                                                <  1:3117681/149‑1 (MQ=255)
                                 ttCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAg                                                                                                               <  2:1534646/149‑1 (MQ=255)
                                  tCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAgg                                                                                                              <  1:644454/149‑1 (MQ=255)
                                  tCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAgg                                                                                                              <  2:4191053/149‑1 (MQ=255)
                                  tCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAgg                                                                                                              <  2:438122/149‑1 (MQ=255)
                                   cGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAggg                                                                                                             <  1:4160767/149‑1 (MQ=255)
                                    gCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGGTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGAGACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGt                                                                                                            <  2:2570358/149‑1 (MQ=255)
                                      aCGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTa                                                                                                          <  1:671281/149‑1 (MQ=255)
                                       cGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                         <  1:4557172/149‑1 (MQ=255)
                                       cGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                         <  1:1160837/149‑1 (MQ=255)
                                        ggTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATc                                                                                                        >  1:288005/1‑149 (MQ=255)
                                         gTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                       >  2:4459336/1‑149 (MQ=255)
                                         gTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                       >  2:1575258/1‑149 (MQ=255)
                                         gTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                       >  2:4096336/1‑149 (MQ=255)
                                         gTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                       >  2:1480769/1‑149 (MQ=255)
                                            aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                    <  2:1084286/149‑1 (MQ=255)
                                            aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                    <  2:2853644/149‑1 (MQ=255)
                                             tCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                                   >  1:1799323/1‑149 (MQ=255)
                                                 tCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAtt                                                                                               >  1:1723583/1‑149 (MQ=255)
                                                  cATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTa                                                                                              <  1:4593845/149‑1 (MQ=255)
                                                   aTCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                             <  1:3868854/149‑1 (MQ=255)
                                                   aTCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                             >  2:2475790/1‑149 (MQ=255)
                                                    tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                            >  1:3246847/1‑149 (MQ=255)
                                                    tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                            <  2:926947/149‑1 (MQ=255)
                                                    tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATg                                                                                            >  1:3195543/1‑149 (MQ=255)
                                                      tttctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATgcg                                                                                          >  2:2711076/1‑149 (MQ=255)
                                                      tttctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAGTGATTATgcg                                                                                          >  1:4336470/1‑149 (MQ=255)
                                                        tctttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCa                                                                                        <  2:1772707/149‑1 (MQ=255)
                                                              gCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAc                                                                                  >  2:3357863/1‑149 (MQ=255)
                                                               cAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAcc                                                                                 <  1:3520229/149‑1 (MQ=255)
                                                                aaTGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAccc                                                                                >  2:704726/1‑149 (MQ=255)
                                                                 aTGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAcccc                                                                               <  1:3799938/149‑1 (MQ=255)
                                                                 aTGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAcccc                                                                               >  1:4439036/1‑149 (MQ=255)
                                                                   ggCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTCTGCGCATTTTACCCcac                                                                             >  1:2427439/1‑149 (MQ=255)
                                                                     cACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACaa                                                                           <  2:3456987/149‑1 (MQ=255)
                                                                      aCCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAGTATGCGCATTTTACCCCACAAg                                                                          >  1:4310008/1‑149 (MQ=255)
                                                                           aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCCCGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaaa                                                                     >  1:2666904/1‑149 (MQ=255)
                                                                           aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaaa                                                                     >  2:4497682/1‑149 (MQ=255)
                                                                              cacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTc                                                                  >  2:417380/1‑149 (MQ=255)
                                                                              cacTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTc                                                                  >  1:1745926/1‑149 (MQ=255)
                                                                                cTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAg                                                                >  1:4661675/1‑149 (MQ=255)
                                                                                 tGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGc                                                               >  2:2066193/1‑149 (MQ=255)
                                                                                 tGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGc                                                               <  1:4255329/149‑1 (MQ=255)
                                                                                  gCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCa                                                              >  2:359823/1‑149 (MQ=255)
                                                                                  gCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACACCACAAGCAAAATTCAGCa                                                              >  2:302536/1‑149 (MQ=255)
                                                                                     cGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGc                                                           <  2:4134742/149‑1 (MQ=255)
                                                                                      gTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGcc                                                          <  2:3974361/149‑1 (MQ=255)
                                                                                      gTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGcc                                                          >  2:3569234/1‑149 (MQ=255)
                                                                                          cTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTc                                                      <  1:1529466/149‑1 (MQ=255)
                                                                                             aagaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTgg                                                   >  1:1092428/1‑149 (MQ=255)
                                                                                              agaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGa                                                  <  2:258090/149‑1 (MQ=255)
                                                                                               gaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAGGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                                 >  1:3897710/1‑149 (MQ=255)
                                                                                                aaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATa                                                >  1:986467/1‑149 (MQ=255)
                                                                                                 aTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATaa                                               <  2:1179019/149‑1 (MQ=255)
                                                                                                 aTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGATATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGACTGGATaa                                               >  1:4028242/1‑149 (MQ=255)
                                                                                                  tCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGCTAAg                                              >  2:2540423/1‑149 (MQ=255)
                                                                                                  tCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAg                                              >  1:1198635/1‑149 (MQ=255)
                                                                                                  tCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAg                                              >  1:540270/1‑149 (MQ=255)
                                                                                                     ttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                           <  2:2061872/149‑1 (MQ=255)
                                                                                                     ttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                           <  1:1666662/149‑1 (MQ=255)
                                                                                                     ttGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                           <  1:1965515/149‑1 (MQ=255)
                                                                                                       gCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAgg                                         >  2:3082803/1‑149 (MQ=255)
                                                                                                                   gcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                             >  2:837208/1‑149 (MQ=255)
                                                                                                                   gcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                             <  2:3904519/149‑1 (MQ=255)
                                                                                                                       cATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGc                         <  2:4623182/149‑1 (MQ=255)
                                                                                                                         tCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTg                       >  1:2722909/1‑149 (MQ=255)
                                                                                                                              cATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCt                  <  2:2068743/149‑1 (MQ=255)
                                                                                                                               aTTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTc                 >  2:1873174/1‑149 (MQ=255)
                                                                                                                                 tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCaa               >  1:2246906/1‑149 (MQ=255)
                                                                                                                                  aCTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAAc              >  1:1398023/1‑149 (MQ=255)
                                                                                                                                     ggATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGcc           <  1:3652394/149‑1 (MQ=255)
                                                                                                                                       aTACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTc         >  1:376429/1‑149 (MQ=255)
                                                                                                                                       aTACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTc         <  2:3466011/149‑1 (MQ=255)
                                                                                                                                         aCGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAc       <  2:3246847/149‑1 (MQ=255)
                                                                                                                                           gCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt     <  1:2859054/149‑1 (MQ=255)
                                                                                                                                           gCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCt     <  1:948201/149‑1 (MQ=255)
                                                                                                                                              cAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaac  <  1:3301883/149‑1 (MQ=255)
                                                                                                                                              cAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTaac  <  2:4131922/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACCTAAC  >  CP009273/104415‑104705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑